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The map label for this gene is hutH

Identifier: 27365708

GI number: 27365708

Start: 2426446

End: 2427987

Strand: Reverse

Name: hutH

Synonym: VV1_2389

Alternate gene names: 27365708

Gene position: 2427987-2426446 (Counterclockwise)

Preceding gene: 27365709

Following gene: 326423998

Centisome position: 73.98

GC content: 51.23

Gene sequence:

>1542_bases
ATGGATATGTTAAATCTCACGCTAAAGCCCGGTTGTCTTAGCTTAAATCAACTGCGTCAAGTCAGTCGTTCCCCGATCAA
TCTTTCTCTTGATGCGTCCGCGATTCCAGCCATTGAAGAGAGCACTCAAGTGGTTGAACGAGTCATCGCCGAGGATCGCA
CCGTTTACGGCATTAATACTGGGTTTGGCTTATTGGCCAATACACGCATCGCCCCAGAAGATCTTGAAACACTGCAACGC
AGTATCGTCCTTTCTCACGCAGCGGGTATCGGCGAGTTCATGGCAGATGAAACGGTACGTCTGATGATGGTGCTCAAAAT
CAATAGCCTATCCCGTGGTTATTCCGGTATTCGTCTCAACGTCATTCAAATGCTGATCGATTTGGTCAACGCGCAAGTTT
ATCCTTGTGTACCACAAAAAGGCTCGGTTGGGGCATCGGGCGACTTGGCCCCACTGGCTCATATGAGCACAGTGTTACTG
GGTGAAGGGCAAGCGCGTCACAACGGCAAAATTATCTCAGGCCTAGAAGCACTCAAAATTGCTGGCCTTGAACCGATCAC
GCTTGCACCAAAAGAAGGTCTGGCACTACTGAATGGCACACAGGCCTCGACGGCTTTCGCGTTGGAAGGTCTGTTTATCG
CTGAAGACCTCTTCGCATCCGCCACCGTCTGCGGCGCAATGTCAGTCGAAGCTGCGTTAGGGAGTCGCCGTCCTTTTGAC
CCGCGTATTCACCGCGTGCGCGGACACCGTAGCCAAATGGATTCGGCGATGGCCTATCGTCATCTACTGGACACCAGTAG
TGAAATTGGCCAGTCACACAGCAACTGTGAAAAGGTGCAAGACCCTTATTCGTTGCGTTGCCAGCCGCAAGTGATGGGCG
CATGTCTGCAGCAGATCCGTAACTCAGCTGAGATCTTGTTGGTTGAGTCGAACTCGGTTTCCGACAATCCCCTCGTATTT
GCTGAAGATGACGACATCATTTCTGGTGGTAACTTCCACGCGGAGCCTGTTGCTATGGCAGCAGACAACCTTGCTCTCGC
GATTGCAGAAATCGGTAGCCTCTCAGAGCGCCGCATGGCATTGCTGATTGATAGCGCACTCTCTAAGCTGCCACCATTTT
TGGTCGATAACGGCGGAGTCAACTCTGGTTTTATGATTGCGCAAGTGACCTCAGCGGCGTTGGCAAGTGAAAACAAAACT
CTTGCACACCCGGCATCCGTGGATAGCTTACCAACGTCAGCGAACCAAGAAGATCATGTGTCCATGGCCACCTTTGCCGC
CCGTCGTTTACGTGAAATGGGTGAAAACACACGTGGCATCTTAGCGGTAGAGTATCTCTCAGCGGCGCAAGGACTGGATT
TCCGTGCACCACATAAATCCTCACCTCGCATAGAACAAGCAAAGCAGATGCTACGAGAGAAAGTCAGTTTCTATGATAAA
GACCGTTACTTTGCACCGGATATTGAAAAGGCCAACTCACTGCTAAAGCTGGCCATGCACAACGTGCTGATGCCAGAAGC
GCTGTTACCGAGTGTTCTCTAA

Upstream 100 bases:

>100_bases
ACGCCGATGCGGGTTACGACATCGCCAAACAGTGCGCAGCGCAGCAAGGTCTTGACCTGCCGATGCTGAACGAAGAATTG
AGCAAGTTGAAGTAAGGAAT

Downstream 100 bases:

>100_bases
TCACGGATAAGCAAACCATAACCACAAGAGGGCATAACTTGCCCTCTTGCTTCTTGTTTCCAGATGTAAACCGTTCACTT
TGCACCCAAATCTGTGAGCA

Product: histidine ammonia-lyase

Products: NA

Alternate protein names: Histidase

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MDMLNLTLKPGCLSLNQLRQVSRSPINLSLDASAIPAIEESTQVVERVIAEDRTVYGINTGFGLLANTRIAPEDLETLQR
SIVLSHAAGIGEFMADETVRLMMVLKINSLSRGYSGIRLNVIQMLIDLVNAQVYPCVPQKGSVGASGDLAPLAHMSTVLL
GEGQARHNGKIISGLEALKIAGLEPITLAPKEGLALLNGTQASTAFALEGLFIAEDLFASATVCGAMSVEAALGSRRPFD
PRIHRVRGHRSQMDSAMAYRHLLDTSSEIGQSHSNCEKVQDPYSLRCQPQVMGACLQQIRNSAEILLVESNSVSDNPLVF
AEDDDIISGGNFHAEPVAMAADNLALAIAEIGSLSERRMALLIDSALSKLPPFLVDNGGVNSGFMIAQVTSAALASENKT
LAHPASVDSLPTSANQEDHVSMATFAARRLREMGENTRGILAVEYLSAAQGLDFRAPHKSSPRIEQAKQMLREKVSFYDK
DRYFAPDIEKANSLLKLAMHNVLMPEALLPSVL

Sequences:

>Translated_513_residues
MDMLNLTLKPGCLSLNQLRQVSRSPINLSLDASAIPAIEESTQVVERVIAEDRTVYGINTGFGLLANTRIAPEDLETLQR
SIVLSHAAGIGEFMADETVRLMMVLKINSLSRGYSGIRLNVIQMLIDLVNAQVYPCVPQKGSVGASGDLAPLAHMSTVLL
GEGQARHNGKIISGLEALKIAGLEPITLAPKEGLALLNGTQASTAFALEGLFIAEDLFASATVCGAMSVEAALGSRRPFD
PRIHRVRGHRSQMDSAMAYRHLLDTSSEIGQSHSNCEKVQDPYSLRCQPQVMGACLQQIRNSAEILLVESNSVSDNPLVF
AEDDDIISGGNFHAEPVAMAADNLALAIAEIGSLSERRMALLIDSALSKLPPFLVDNGGVNSGFMIAQVTSAALASENKT
LAHPASVDSLPTSANQEDHVSMATFAARRLREMGENTRGILAVEYLSAAQGLDFRAPHKSSPRIEQAKQMLREKVSFYDK
DRYFAPDIEKANSLLKLAMHNVLMPEALLPSVL
>Mature_513_residues
MDMLNLTLKPGCLSLNQLRQVSRSPINLSLDASAIPAIEESTQVVERVIAEDRTVYGINTGFGLLANTRIAPEDLETLQR
SIVLSHAAGIGEFMADETVRLMMVLKINSLSRGYSGIRLNVIQMLIDLVNAQVYPCVPQKGSVGASGDLAPLAHMSTVLL
GEGQARHNGKIISGLEALKIAGLEPITLAPKEGLALLNGTQASTAFALEGLFIAEDLFASATVCGAMSVEAALGSRRPFD
PRIHRVRGHRSQMDSAMAYRHLLDTSSEIGQSHSNCEKVQDPYSLRCQPQVMGACLQQIRNSAEILLVESNSVSDNPLVF
AEDDDIISGGNFHAEPVAMAADNLALAIAEIGSLSERRMALLIDSALSKLPPFLVDNGGVNSGFMIAQVTSAALASENKT
LAHPASVDSLPTSANQEDHVSMATFAARRLREMGENTRGILAVEYLSAAQGLDFRAPHKSSPRIEQAKQMLREKVSFYDK
DRYFAPDIEKANSLLKLAMHNVLMPEALLPSVL

Specific function: Unknown

COG id: COG2986

COG function: function code E; Histidine ammonia-lyase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAL/histidase family

Homologues:

Organism=Homo sapiens, GI4504333, Length=487, Percent_Identity=43.9425051334702, Blast_Score=410, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17567831, Length=492, Percent_Identity=44.3089430894309, Blast_Score=385, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HUTH_VIBVU (Q8DA21)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_761236.1
- ProteinModelPortal:   Q8DA21
- SMR:   Q8DA21
- GeneID:   1179264
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_2389
- HOGENOM:   HBG510887
- OMA:   QKGQIDS
- ProtClustDB:   PRK09367
- BioCyc:   VVUL216895:VV1_2389-MONOMER
- BRENDA:   4.3.1.3
- GO:   GO:0005737
- HAMAP:   MF_00229
- InterPro:   IPR005921
- InterPro:   IPR008948
- InterPro:   IPR001106
- InterPro:   IPR022313
- TIGRFAMs:   TIGR01225

Pfam domain/function: PF00221 PAL; SSF48557 L-Aspartase-like

EC number: =4.3.1.3

Molecular weight: Translated: 55205; Mature: 55205

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00488 PAL_HISTIDASE; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMLNLTLKPGCLSLNQLRQVSRSPINLSLDASAIPAIEESTQVVERVIAEDRTVYGINT
CCCEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECC
GFGLLANTRIAPEDLETLQRSIVLSHAAGIGEFMADETVRLMMVLKINSLSRGYSGIRLN
CCCHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHEEEEEEEEEHHHCCCCCCHHHH
VIQMLIDLVNAQVYPCVPQKGSVGASGDLAPLAHMSTVLLGEGQARHNGKIISGLEALKI
HHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
AGLEPITLAPKEGLALLNGTQASTAFALEGLFIAEDLFASATVCGAMSVEAALGSRRPFD
CCCCCEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PRIHRVRGHRSQMDSAMAYRHLLDTSSEIGQSHSNCEKVQDPYSLRCQPQVMGACLQQIR
HHHHHHHCHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHC
NSAEILLVESNSVSDNPLVFAEDDDIISGGNFHAEPVAMAADNLALAIAEIGSLSERRMA
CCCEEEEEECCCCCCCCEEEECCCCEECCCCCCCCCHHHHHCCHHHHHHHHCCCHHHHHH
LLIDSALSKLPPFLVDNGGVNSGFMIAQVTSAALASENKTLAHPASVDSLPTSANQEDHV
HHHHHHHHHCCCEEEECCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
SMATFAARRLREMGENTRGILAVEYLSAAQGLDFRAPHKSSPRIEQAKQMLREKVSFYDK
HHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
DRYFAPDIEKANSLLKLAMHNVLMPEALLPSVL
CCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCC
>Mature Secondary Structure
MDMLNLTLKPGCLSLNQLRQVSRSPINLSLDASAIPAIEESTQVVERVIAEDRTVYGINT
CCCEEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECC
GFGLLANTRIAPEDLETLQRSIVLSHAAGIGEFMADETVRLMMVLKINSLSRGYSGIRLN
CCCHHCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHEEEEEEEEEHHHCCCCCCHHHH
VIQMLIDLVNAQVYPCVPQKGSVGASGDLAPLAHMSTVLLGEGQARHNGKIISGLEALKI
HHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
AGLEPITLAPKEGLALLNGTQASTAFALEGLFIAEDLFASATVCGAMSVEAALGSRRPFD
CCCCCEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PRIHRVRGHRSQMDSAMAYRHLLDTSSEIGQSHSNCEKVQDPYSLRCQPQVMGACLQQIR
HHHHHHHCHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHC
NSAEILLVESNSVSDNPLVFAEDDDIISGGNFHAEPVAMAADNLALAIAEIGSLSERRMA
CCCEEEEEECCCCCCCCEEEECCCCEECCCCCCCCCHHHHHCCHHHHHHHHCCCHHHHHH
LLIDSALSKLPPFLVDNGGVNSGFMIAQVTSAALASENKTLAHPASVDSLPTSANQEDHV
HHHHHHHHHCCCEEEECCCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
SMATFAARRLREMGENTRGILAVEYLSAAQGLDFRAPHKSSPRIEQAKQMLREKVSFYDK
HHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
DRYFAPDIEKANSLLKLAMHNVLMPEALLPSVL
CCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA