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The map label for this gene is azoR

Identifier: 27365661

GI number: 27365661

Start: 2367930

End: 2368517

Strand: Reverse

Name: azoR

Synonym: VV1_2341

Alternate gene names: 27365661

Gene position: 2368517-2367930 (Counterclockwise)

Preceding gene: 27365662

Following gene: 27365648

Centisome position: 72.17

GC content: 47.96

Gene sequence:

>588_bases
ATGTCTCGTTTACTAGTATTAAAATCAAGCATTCTTGGCGATTACTCACAATCAAACAAACTGGTTGACGAGTTCATCAA
CCATTTCGACCACCAGCAGCTCGTTGTACGCGATCTGGCGCAGCAACCATTGCCGGTTTTAGATTTTCAGGTGGCAACCG
CTCTGCGCGCTGCGGGTGAGTTGACTGCCGAGCAACAAGCAATTGTGACATTGTCAGACACCTTGATTGATGAAATCAAA
GAGGCAGACACCCTAGTGATTGCGGCACCTATGTACAACTTCACCATCCCGACTCAATTGAAAAACTGGATCGATTTGAT
TGCGCGTGCAGGGGTGACATTCACTTACACCGAGCAAGGCCCGAAAGGTCTGATTGAAGGCAAGAAAGCCGTGATCGTGA
CAACACGCGGCGGGATTCATAAAGACGCAGCTTCTGACATCATTACACCGTACTTGAAAACCGTACTGGGTTTTGTTGGC
ATCACAGAGGTTGAATTTGTCTACGCTGAAGCGCTGAACATGGGTGACGATTTTGCCAATAAAGGTTTGGCTTCAGCGAG
TGAGCACCTTGCAGCGCTAACAGCTTAA

Upstream 100 bases:

>100_bases
CAGTTTTTTCGAATGAGATGAGCGAATTTCAACGCTTCATCCCTAAGATGAACTCGATAGAATGACTTCATCGTTTGAAC
ACCCATCTAAGGAATTCACT

Downstream 100 bases:

>100_bases
GTACGCTAACAACTTAAGTCACAGCACCCGTATAGATTCCACTGCCTGGCACACCAACGGATTGATAGCACCTTGCCCAC
TTCGTGCCAGTTTGTTTACG

Product: azoreductase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSRLLVLKSSILGDYSQSNKLVDEFINHFDHQQLVVRDLAQQPLPVLDFQVATALRAAGELTAEQQAIVTLSDTLIDEIK
EADTLVIAAPMYNFTIPTQLKNWIDLIARAGVTFTYTEQGPKGLIEGKKAVIVTTRGGIHKDAASDIITPYLKTVLGFVG
ITEVEFVYAEALNMGDDFANKGLASASEHLAALTA

Sequences:

>Translated_195_residues
MSRLLVLKSSILGDYSQSNKLVDEFINHFDHQQLVVRDLAQQPLPVLDFQVATALRAAGELTAEQQAIVTLSDTLIDEIK
EADTLVIAAPMYNFTIPTQLKNWIDLIARAGVTFTYTEQGPKGLIEGKKAVIVTTRGGIHKDAASDIITPYLKTVLGFVG
ITEVEFVYAEALNMGDDFANKGLASASEHLAALTA
>Mature_194_residues
SRLLVLKSSILGDYSQSNKLVDEFINHFDHQQLVVRDLAQQPLPVLDFQVATALRAAGELTAEQQAIVTLSDTLIDEIKE
ADTLVIAAPMYNFTIPTQLKNWIDLIARAGVTFTYTEQGPKGLIEGKKAVIVTTRGGIHKDAASDIITPYLKTVLGFVGI
TEVEFVYAEALNMGDDFANKGLASASEHLAALTA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family

Homologues:

Organism=Escherichia coli, GI1787680, Length=191, Percent_Identity=56.020942408377, Blast_Score=219, Evalue=7e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AZOR_VIBVU (Q8DA68)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_761189.1
- ProteinModelPortal:   Q8DA68
- SMR:   Q8DA68
- GeneID:   1179217
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_2341
- HOGENOM:   HBG565834
- OMA:   YFDFIAR
- ProtClustDB:   PRK00170
- BioCyc:   VVUL216895:VV1_2341-MONOMER
- HAMAP:   MF_01216
- InterPro:   IPR003680
- InterPro:   IPR023048

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 3.1.4.14

Molecular weight: Translated: 21175; Mature: 21044

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLLVLKSSILGDYSQSNKLVDEFINHFDHQQLVVRDLAQQPLPVLDFQVATALRAAGE
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LTAEQQAIVTLSDTLIDEIKEADTLVIAAPMYNFTIPTQLKNWIDLIARAGVTFTYTEQG
HCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCC
PKGLIEGKKAVIVTTRGGIHKDAASDIITPYLKTVLGFVGITEVEFVYAEALNMGDDFAN
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHH
KGLASASEHLAALTA
CCHHHHHHHHHHHCC
>Mature Secondary Structure 
SRLLVLKSSILGDYSQSNKLVDEFINHFDHQQLVVRDLAQQPLPVLDFQVATALRAAGE
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LTAEQQAIVTLSDTLIDEIKEADTLVIAAPMYNFTIPTQLKNWIDLIARAGVTFTYTEQG
HCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCC
PKGLIEGKKAVIVTTRGGIHKDAASDIITPYLKTVLGFVGITEVEFVYAEALNMGDDFAN
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHH
KGLASASEHLAALTA
CCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA