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The map label for this gene is mnmC

Identifier: 27365330

GI number: 27365330

Start: 1977000

End: 1979018

Strand: Reverse

Name: mnmC

Synonym: VV1_1985

Alternate gene names: 27365330

Gene position: 1979018-1977000 (Counterclockwise)

Preceding gene: 27365341

Following gene: 27365327

Centisome position: 60.3

GC content: 49.53

Gene sequence:

>2019_bases
ATGACATCCATTACCCATGCAGAACTCGGCTGGAATGAAGTCGGCACACCGGTTTCTGACCAATTTGATGACGTTTACTT
CTCCAATGTGAACGGCCTAGAAGAAACACGCTATGTGTTTCTTAAACAAAATCTTATTCCTGAAAGATGGCAAGAATTTG
ACCGCCGTCGCTTTGTGATTGGCGAAACGGGTTTTGGCACTGGATTGAATTTCCTCGCGGTTTGGCAGGCGTTCAATGAC
TTTCGCCGTGCAAACCCCGACGCAACACTCAAAGAGTTACATTTTGTCAGCTTTGAAAAGTTTCCCCTTAGCAAACAAGA
TTTGATCAAAGCGCATCAAGCTTGGCCTGAGTTGGCGGAACTCGCGGAAAAACTTCATCGTCATTATCCACCTGCTGTTC
CAGAATGTCATCGTATTGTGCTGGACAATGGCGCTGTCACACTTGATCTGTGGCTGGGCGATATTAAAGATTGCCTACCC
TCGGTGCCTTACGGTGAAGAAGGCATTATCGACACTTGGTTCCTCGATGGGTTTGCTCCAAGTAAAAATCCCGAGATGTG
GAATCAGGATCTTTTCAATGGCATGGCGAAACTGGCGAGATCTGAGTGTCGAGTGGCAACGTTTACCTCTGCGGGTTTTG
TAAGACGAGGCTTGATTGAAGCGGGCTTTGCCATGAAAAAAGTGAAAGGCTTTGGCACCAAGCGAGAAATGATCGCAGGC
TGCATGGAAACAAGACAACAACAAAGTCGCCACGCGCCTTACTTCAATCGCACCTCTGCATCTCATCTCGATTCTATTGC
CATCATTGGCGGAGGAATTGCCAGCGCAGCCCTCGCAAAAGCCTTGGTTCAACGTGGGCAAAAAGTAACCCTCTATTGTA
AACACGCCCAAGCTGCAGAAGGCGCATCGGGTAACCGACAAGGCGCAGTTTACCCTTTACTCAATGGCAATCATGATGGC
GTTTCACGCGTATTTGCCCCCGCGTTTTTGTTTGCTCGCCAGTTTGTAGAGCAAGCCGCACAAGCGCTCACCTTCGATCA
TGATTGGTGTGGCGTGACCCAGTTGATGTGGGATGAAAAATCAACCAACAAGTTGGACAAGATGCTCTCTGGGAACTTTG
CACCCGAGTTGATTCAAAAGCTCTCCGTAGGAGAAACCGCAGCAAAAATTGGTTTGCCGATCGACATGGCATCGGTTCAT
TATCCACTTGGCGGCTGGCTATGCCCGGCAGAGCTAACCCAAGCCCTCTTTACGCAATTGGGCACCCTCGACAACTTCAC
GGCCAAATTTGAGCAGAGTGTTGAACAACTCATCTGGGACGAAAGGTCACAGCAATGGCAAGTCCATACTCAGGGCCAAC
ACGATACTTATAGCGCTGTGGTTATTGCCAATGGTCATGAGTTCCAAACCTTCAGCCAAACCGCTGACATTCCGCTTGGC
CAAGTCAAAGGTCAAGTCAGCCATGTCCCTGCTACTGAGACGCTTTCTAAGCTCAAAAGTGTGCTGTGTTACGACGGCTA
CATGACTCCCGTCAACCCAAACAATCAGCATCTTTGCATTGGCGCAAGTTACGATCGCCGCCATTTGGATACTGAGTTTG
ATGTCAATGCGCAACAAGAGAATGCCGAGAGACTGACGCAATGTGTCCCAAACCAAGCGTGGGCAAAAGAAGTGGATACA
TCTGGCAATTTATCTCGCCAAGGGATCCGCTGTGTGAGCCGAGATCATCTGCCGTTTGTTGGCAATGTCGGCGACTTCTC
AGCCATTAAGCGTCAATACGCCGATTTGCCGCACACTCAAGCAGAAGAGATTGAGGTAATTTCTCAGTTTCCGAACTTAT
TCTGCCTCCTTGGTCTTGGCTCTCGTGGCTTGAGCTCTGCCCCACTGATGGCTGAGTTGCTCGCCTCGCAAATCTGTAAC
GACCCGCTGCCTTTGCCTGTTGACGTGCTTGAAGAGTTACACCCAAGCCGCATGTGGGTAAGAAAACTGCGCAAAGGCAA
AGCCATTACCGAGCTGTAA

Upstream 100 bases:

>100_bases
AGATAATAACTAAGAAGCATAACAAAAGTGGTCAGCTTTCCCAGAAATTCGTACAATCGCCCTATTCTAACCGCGCTACA
TCACAGATTTACATCCTACT

Downstream 100 bases:

>100_bases
GTTGAAGATACGCACAAACAAAAACGCCTCGTTGTATTGACGAGGCGTTTTTATTGAAACAGAGGTTAAGCTTGCGCTTG
GAGCTCTTGCCACAAGTCGC

Product: bifunctional tRNA (mnm(5)s(2)U34)-methyltransferase/FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 672; Mature: 671

Protein sequence:

>672_residues
MTSITHAELGWNEVGTPVSDQFDDVYFSNVNGLEETRYVFLKQNLIPERWQEFDRRRFVIGETGFGTGLNFLAVWQAFND
FRRANPDATLKELHFVSFEKFPLSKQDLIKAHQAWPELAELAEKLHRHYPPAVPECHRIVLDNGAVTLDLWLGDIKDCLP
SVPYGEEGIIDTWFLDGFAPSKNPEMWNQDLFNGMAKLARSECRVATFTSAGFVRRGLIEAGFAMKKVKGFGTKREMIAG
CMETRQQQSRHAPYFNRTSASHLDSIAIIGGGIASAALAKALVQRGQKVTLYCKHAQAAEGASGNRQGAVYPLLNGNHDG
VSRVFAPAFLFARQFVEQAAQALTFDHDWCGVTQLMWDEKSTNKLDKMLSGNFAPELIQKLSVGETAAKIGLPIDMASVH
YPLGGWLCPAELTQALFTQLGTLDNFTAKFEQSVEQLIWDERSQQWQVHTQGQHDTYSAVVIANGHEFQTFSQTADIPLG
QVKGQVSHVPATETLSKLKSVLCYDGYMTPVNPNNQHLCIGASYDRRHLDTEFDVNAQQENAERLTQCVPNQAWAKEVDT
SGNLSRQGIRCVSRDHLPFVGNVGDFSAIKRQYADLPHTQAEEIEVISQFPNLFCLLGLGSRGLSSAPLMAELLASQICN
DPLPLPVDVLEELHPSRMWVRKLRKGKAITEL

Sequences:

>Translated_672_residues
MTSITHAELGWNEVGTPVSDQFDDVYFSNVNGLEETRYVFLKQNLIPERWQEFDRRRFVIGETGFGTGLNFLAVWQAFND
FRRANPDATLKELHFVSFEKFPLSKQDLIKAHQAWPELAELAEKLHRHYPPAVPECHRIVLDNGAVTLDLWLGDIKDCLP
SVPYGEEGIIDTWFLDGFAPSKNPEMWNQDLFNGMAKLARSECRVATFTSAGFVRRGLIEAGFAMKKVKGFGTKREMIAG
CMETRQQQSRHAPYFNRTSASHLDSIAIIGGGIASAALAKALVQRGQKVTLYCKHAQAAEGASGNRQGAVYPLLNGNHDG
VSRVFAPAFLFARQFVEQAAQALTFDHDWCGVTQLMWDEKSTNKLDKMLSGNFAPELIQKLSVGETAAKIGLPIDMASVH
YPLGGWLCPAELTQALFTQLGTLDNFTAKFEQSVEQLIWDERSQQWQVHTQGQHDTYSAVVIANGHEFQTFSQTADIPLG
QVKGQVSHVPATETLSKLKSVLCYDGYMTPVNPNNQHLCIGASYDRRHLDTEFDVNAQQENAERLTQCVPNQAWAKEVDT
SGNLSRQGIRCVSRDHLPFVGNVGDFSAIKRQYADLPHTQAEEIEVISQFPNLFCLLGLGSRGLSSAPLMAELLASQICN
DPLPLPVDVLEELHPSRMWVRKLRKGKAITEL
>Mature_671_residues
TSITHAELGWNEVGTPVSDQFDDVYFSNVNGLEETRYVFLKQNLIPERWQEFDRRRFVIGETGFGTGLNFLAVWQAFNDF
RRANPDATLKELHFVSFEKFPLSKQDLIKAHQAWPELAELAEKLHRHYPPAVPECHRIVLDNGAVTLDLWLGDIKDCLPS
VPYGEEGIIDTWFLDGFAPSKNPEMWNQDLFNGMAKLARSECRVATFTSAGFVRRGLIEAGFAMKKVKGFGTKREMIAGC
METRQQQSRHAPYFNRTSASHLDSIAIIGGGIASAALAKALVQRGQKVTLYCKHAQAAEGASGNRQGAVYPLLNGNHDGV
SRVFAPAFLFARQFVEQAAQALTFDHDWCGVTQLMWDEKSTNKLDKMLSGNFAPELIQKLSVGETAAKIGLPIDMASVHY
PLGGWLCPAELTQALFTQLGTLDNFTAKFEQSVEQLIWDERSQQWQVHTQGQHDTYSAVVIANGHEFQTFSQTADIPLGQ
VKGQVSHVPATETLSKLKSVLCYDGYMTPVNPNNQHLCIGASYDRRHLDTEFDVNAQQENAERLTQCVPNQAWAKEVDTS
GNLSRQGIRCVSRDHLPFVGNVGDFSAIKRQYADLPHTQAEEIEVISQFPNLFCLLGLGSRGLSSAPLMAELLASQICND
PLPLPVDVLEELHPSRMWVRKLRKGKAITEL

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=668, Percent_Identity=52.2455089820359, Blast_Score=676, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_VIBVU (Q8DB38)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_760858.1
- ProteinModelPortal:   Q8DB38
- SMR:   Q8DB38
- GeneID:   1178884
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_1985
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- ProtClustDB:   PRK01747
- BioCyc:   VVUL216895:VV1_1985-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 74968; Mature: 74837

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSITHAELGWNEVGTPVSDQFDDVYFSNVNGLEETRYVFLKQNLIPERWQEFDRRRFVI
CCCCCCCCCCCHHCCCCCCCCCCCHHHCCCCCCHHHHEEHHHHCCCHHHHHHHHCCEEEE
GETGFGTGLNFLAVWQAFNDFRRANPDATLKELHFVSFEKFPLSKQDLIKAHQAWPELAE
ECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHH
LAEKLHRHYPPAVPECHRIVLDNGAVTLDLWLGDIKDCLPSVPYGEEGIIDTWFLDGFAP
HHHHHHHHCCCCCCCHHEEEECCCCEEEEEHHHHHHHHCCCCCCCCCCCEEEEEECCCCC
SKNPEMWNQDLFNGMAKLARSECRVATFTSAGFVRRGLIEAGFAMKKVKGFGTKREMIAG
CCCCCHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
CMETRQQQSRHAPYFNRTSASHLDSIAIIGGGIASAALAKALVQRGQKVTLYCKHAQAAE
HHHHHHHHHHCCCCCCCCCHHHHCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCC
GASGNRQGAVYPLLNGNHDGVSRVFAPAFLFARQFVEQAAQALTFDHDWCGVTQLMWDEK
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
STNKLDKMLSGNFAPELIQKLSVGETAAKIGLPIDMASVHYPLGGWLCPAELTQALFTQL
HHHHHHHHHCCCCCHHHHHHHCCCCHHHHHCCCEEHHHHCCCCCCEECHHHHHHHHHHHH
GTLDNFTAKFEQSVEQLIWDERSQQWQVHTQGQHDTYSAVVIANGHEFQTFSQTADIPLG
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCCHHHHCCCCCHH
QVKGQVSHVPATETLSKLKSVLCYDGYMTPVNPNNQHLCIGASYDRRHLDTEFDVNAQQE
HHCCCCCCCCHHHHHHHHHHHHHHCCEECCCCCCCCEEEEECCCCHHHCCCCCCCCCCHH
NAERLTQCVPNQAWAKEVDTSGNLSRQGIRCVSRDHLPFVGNVGDFSAIKRQYADLPHTQ
HHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCC
AEEIEVISQFPNLFCLLGLGSRGLSSAPLMAELLASQICNDPLPLPVDVLEELHPSRMWV
HHHHHHHHHCCHHEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHH
RKLRKGKAITEL
HHHHCCCCCCCC
>Mature Secondary Structure 
TSITHAELGWNEVGTPVSDQFDDVYFSNVNGLEETRYVFLKQNLIPERWQEFDRRRFVI
CCCCCCCCCCHHCCCCCCCCCCCHHHCCCCCCHHHHEEHHHHCCCHHHHHHHHCCEEEE
GETGFGTGLNFLAVWQAFNDFRRANPDATLKELHFVSFEKFPLSKQDLIKAHQAWPELAE
ECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHH
LAEKLHRHYPPAVPECHRIVLDNGAVTLDLWLGDIKDCLPSVPYGEEGIIDTWFLDGFAP
HHHHHHHHCCCCCCCHHEEEECCCCEEEEEHHHHHHHHCCCCCCCCCCCEEEEEECCCCC
SKNPEMWNQDLFNGMAKLARSECRVATFTSAGFVRRGLIEAGFAMKKVKGFGTKREMIAG
CCCCCHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
CMETRQQQSRHAPYFNRTSASHLDSIAIIGGGIASAALAKALVQRGQKVTLYCKHAQAAE
HHHHHHHHHHCCCCCCCCCHHHHCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCC
GASGNRQGAVYPLLNGNHDGVSRVFAPAFLFARQFVEQAAQALTFDHDWCGVTQLMWDEK
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCC
STNKLDKMLSGNFAPELIQKLSVGETAAKIGLPIDMASVHYPLGGWLCPAELTQALFTQL
HHHHHHHHHCCCCCHHHHHHHCCCCHHHHHCCCEEHHHHCCCCCCEECHHHHHHHHHHHH
GTLDNFTAKFEQSVEQLIWDERSQQWQVHTQGQHDTYSAVVIANGHEFQTFSQTADIPLG
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCCHHHHCCCCCHH
QVKGQVSHVPATETLSKLKSVLCYDGYMTPVNPNNQHLCIGASYDRRHLDTEFDVNAQQE
HHCCCCCCCCHHHHHHHHHHHHHHCCEECCCCCCCCEEEEECCCCHHHCCCCCCCCCCHH
NAERLTQCVPNQAWAKEVDTSGNLSRQGIRCVSRDHLPFVGNVGDFSAIKRQYADLPHTQ
HHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCC
AEEIEVISQFPNLFCLLGLGSRGLSSAPLMAELLASQICNDPLPLPVDVLEELHPSRMWV
HHHHHHHHHCCHHEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHH
RKLRKGKAITEL
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA