Click here to switch to the map view.

The map label for this gene is vvpD

Identifier: 27364987

GI number: 27364987

Start: 1590249

End: 1591118

Strand: Reverse

Name: vvpD

Synonym: VV1_1623

Alternate gene names: 27364987

Gene position: 1591118-1590249 (Counterclockwise)

Preceding gene: 27364988

Following gene: 326423858

Centisome position: 48.48

GC content: 47.82

Gene sequence:

>870_bases
ATGGACGCGTTACAATACTACCCTTGGCTTTATATTGGTTTAGCTTCCCTGTTTGGCTTACTGGTAGGCAGCTTTCTTAA
CGTCGTCATCTACCGTCTACCGAAAATAATGGAACTGGAATGGCGCCAAGAGTGTGCAGAGTCCTTTCCTGAATACAACA
TCACACCACCGACAGAAACCCTAACATTAAGCACGCCGCGTTCAAGTTGCCCTTCGTGCCATACCCCAATACGAGTTCGA
GATAATATTCCTGTCTTTAGTTGGCTAGCTCTAAGAGGAAAATGTCACCATTGCCAGACGAAAATCAGTGCTCGCTATCC
TTTTGTTGAAGCACTGAGCGCCTTCTTGTGCGGCCTAGTCGCTTGGAAGTTCGGTTATGCACCGATTACCGTAGCACTGA
TTGGTTTTACTTTGGTGTTGATCGCCGCCACCTTCATCGACTTAGATACCATGCTACTGCCTGATCAACTGACACTGCCG
CTGACGTGGACAGGCATCGCGCTAGCGTTACTAGAGATAAGCCCAGTATCTCTGCAAGATTCAGTGTTCGGTGCGATGGC
AGGCTATCTCTGCCTTTGGAGCGTTTATCACCTTTTCAGATTGCTGACAGGGAAAGAAGGCATGGGTTACGGAGACTTTA
AATTGCTTGCGGCACTTGGAGCTTGGCTCGGCTGGCAATATCTACCGATGATCATCTTACTTTCTTCGGTTGTCGGGCTG
ATCTTTGGTCTCATTCAACTCAGATTGCAAAAGCAAGGTATTGAGATGGCTTTTCCATTTGGGCCTTATCTGGCCATTGC
TGGTTGGGTCGCCTTGATGTGGGGAGATAGCTTGATGTCTTGGTATCTCAACTATCTTATAGGGGCGTAA

Upstream 100 bases:

>100_bases
GGGATAAGATAACCCTCGCAGTTTACCTTTTGTAATATCAAGCAAGCTATTGGCTTGCTTGAGCTATTTTTGCCCACACC
ACAAAGAGAACGACGATTAT

Downstream 100 bases:

>100_bases
TATGGCTTTAGTGATTGGATTGACTGGTGGTATCGCCAGCGGAAAAACAACGGTGGCCAATCTTTTTCAACAACACTTTG
CTATCGATATCGTCGACGCA

Product: prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDALQYYPWLYIGLASLFGLLVGSFLNVVIYRLPKIMELEWRQECAESFPEYNITPPTETLTLSTPRSSCPSCHTPIRVR
DNIPVFSWLALRGKCHHCQTKISARYPFVEALSAFLCGLVAWKFGYAPITVALIGFTLVLIAATFIDLDTMLLPDQLTLP
LTWTGIALALLEISPVSLQDSVFGAMAGYLCLWSVYHLFRLLTGKEGMGYGDFKLLAALGAWLGWQYLPMIILLSSVVGL
IFGLIQLRLQKQGIEMAFPFGPYLAIAGWVALMWGDSLMSWYLNYLIGA

Sequences:

>Translated_289_residues
MDALQYYPWLYIGLASLFGLLVGSFLNVVIYRLPKIMELEWRQECAESFPEYNITPPTETLTLSTPRSSCPSCHTPIRVR
DNIPVFSWLALRGKCHHCQTKISARYPFVEALSAFLCGLVAWKFGYAPITVALIGFTLVLIAATFIDLDTMLLPDQLTLP
LTWTGIALALLEISPVSLQDSVFGAMAGYLCLWSVYHLFRLLTGKEGMGYGDFKLLAALGAWLGWQYLPMIILLSSVVGL
IFGLIQLRLQKQGIEMAFPFGPYLAIAGWVALMWGDSLMSWYLNYLIGA
>Mature_289_residues
MDALQYYPWLYIGLASLFGLLVGSFLNVVIYRLPKIMELEWRQECAESFPEYNITPPTETLTLSTPRSSCPSCHTPIRVR
DNIPVFSWLALRGKCHHCQTKISARYPFVEALSAFLCGLVAWKFGYAPITVALIGFTLVLIAATFIDLDTMLLPDQLTLP
LTWTGIALALLEISPVSLQDSVFGAMAGYLCLWSVYHLFRLLTGKEGMGYGDFKLLAALGAWLGWQYLPMIILLSSVVGL
IFGLIQLRLQKQGIEMAFPFGPYLAIAGWVALMWGDSLMSWYLNYLIGA

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family

Homologues:

Organism=Escherichia coli, GI87082194, Length=267, Percent_Identity=41.5730337078652, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI1789732, Length=144, Percent_Identity=45.1388888888889, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEP4_VIBVU (Q56740)

Other databases:

- EMBL:   U48808
- EMBL:   AF070934
- EMBL:   AE016795
- RefSeq:   NP_760515.1
- MEROPS:   A24.001
- GeneID:   1178539
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_1623
- HOGENOM:   HBG710101
- OMA:   VFWLFKL
- ProtClustDB:   CLSK874797
- BioCyc:   VVUL216895:VV1_1623-MONOMER
- BRENDA:   3.4.23.43
- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045
- PRINTS:   PR00864

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24

EC number: =3.4.23.43

Molecular weight: Translated: 32296; Mature: 32296

Theoretical pI: Translated: 6.49; Mature: 6.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc449694)-; HASH(0xe0eff20)-; HASH(0xc655010)-; HASH(0xe44723c)-; HASH(0xdf0b0a0)-; HASH(0xd03c834)-;

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure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HHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9826339