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The map label for this gene is nadB [H]

Identifier: 27364927

GI number: 27364927

Start: 1528909

End: 1530516

Strand: Reverse

Name: nadB [H]

Synonym: VV1_1558

Alternate gene names: 27364927

Gene position: 1530516-1528909 (Counterclockwise)

Preceding gene: 27364942

Following gene: 27364925

Centisome position: 46.64

GC content: 49.88

Gene sequence:

>1608_bases
ATGAACGCAAACCGTGAACATGAATGTGATGTGTTAGTGGTGGGCAGCGGAGCTGCTGGCTTGTCGTTGGCCTTGCGCGT
CGCCAATCACTGCAAAGTTATCGTGTTAAGTAAAGGACCTCGTAGCGAGGGAGCAACCTACTATGCTCAAGGTGGTATCG
CTGCGGTATTCGATGAATCAGACAGTATTGAGTCACACGTTCAAGACACTCTGATCGCCGGAGACGGCATCTGTGAAGAA
GCAACGGTGAAGTTCATCGCTGAAAACGCCAAAGAGTGTGTGCAATGGTTAATTGATGGCGGTGTGCCCTTTGATCGTGA
AGAGGACGACTCTGATGACGAACCGCGTTATCACTTAACCCGCGAAGGCGGCCATAGCCATCGCCGTATTTTGCACGCAG
CCGATGCCACAGGCATGGCGATGCAAACCTCGCTGCAAGACAATGCTCACAACCATCCTAATATTCATGTTTTAGAGCGT
CATAATGCCCTCGACTTGATCACCGAAGACAAAATTGGCGGCGATAAAAATAAAGTGATCGGCGCGTACGTCTGGAACCG
AAATCAAGAGCATGTGGAAACCGTTAAGGCGAAGTTTGTCGTGCTCGCGACCGGTGGCGCATCAAAAGTTTATCAGTACA
CCTCCAACCCAGATGTTTCTTCTGGTGATGGTATTGCGATTGCTTGGCGCGCAGGATGTCGCGTGGCCAATATGGAATTT
AACCAATTCCACCCAACCTGTTTATACCACCCAGAAGCTCGCAACTTCCTCCTGACAGAAGCATTACGTGGTGAAGGCGC
CTATTTACGTCGTCCGGATGGTTCACGCTTCATGCCCGATTTTGACCCGCGTGAAGAGCTGGCTCCTCGTGATGTCGTGG
CACGAGCAATTGACTTCGAAATGAAACGTCTTGGCGCTGACTGCATGTACCTTGATATCAGCCACAAACCCGCTGAGTTC
ATTACCAAACACTTCCCAATGATCCACTCACGTCTGATGGATTTGGGCATTGATATGACCAAAGAGCCGATCCCCATCGT
TCCGGCCGCACATTACACCTGTGGCGGTGTGATGGTCGATACCAATGGGCAAACAGATTTGCACAATCTCTATGCCATCG
GCGAAGTGAGCTACACTGGCCTACACGGAGCAAACCGTATGGCTTCAAATTCCTTGTTGGAGTGTGTGGTTTATGCATGG
GCGGCAGCAAAAGACATTTTGAAAAAACACAAACAAGTCGACTTAGCGTCCAGCTTACCAAGCTGGGACGAAAGCCAAGT
GACCTGCAGTGATGAAGAAGTGATCATCCAACACAACTGGCATGAGCTGCGACTGTTTATGTGGGATTACATGGGTATTG
TACGCACTGACAAGCGCCTTGAGCGTGCACTGCGTCGCATTCAAATGTTGCAACAAGAGACGCATGAATACTACAGTAAC
TTCCGCGTATCCAATAACTTATTGGAGCTGCGTAACTTACTGCAAGTCGCAGAGCTGATGGTTCGTTGTGCGATGCAAAG
GAAAGAGAGCCGCGGTTTACACTACACACTCGATTACCCTGAGCAACTTGAAAACAGTGGCCCGACGATTTTGCGCCCAG
AAGAGTAA

Upstream 100 bases:

>100_bases
GCCGATGTAAATCGGCGTTTTTGCGTGTACTGGAAGCACATGATGTGCGGATTTATACAAAGTGAACTCATTCACTTTAG
ACCGGGATTTTGAAAATTTT

Downstream 100 bases:

>100_bases
ACCACTTTTATCACTGTTCATCTTTTTTTATGACTTGGGGAACGAATTCAGTTCCCCTTTTTGTTGCCACTGTTTTAGCA
CAATCATCAACTGTCGATAA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MNANREHECDVLVVGSGAAGLSLALRVANHCKVIVLSKGPRSEGATYYAQGGIAAVFDESDSIESHVQDTLIAGDGICEE
ATVKFIAENAKECVQWLIDGGVPFDREEDDSDDEPRYHLTREGGHSHRRILHAADATGMAMQTSLQDNAHNHPNIHVLER
HNALDLITEDKIGGDKNKVIGAYVWNRNQEHVETVKAKFVVLATGGASKVYQYTSNPDVSSGDGIAIAWRAGCRVANMEF
NQFHPTCLYHPEARNFLLTEALRGEGAYLRRPDGSRFMPDFDPREELAPRDVVARAIDFEMKRLGADCMYLDISHKPAEF
ITKHFPMIHSRLMDLGIDMTKEPIPIVPAAHYTCGGVMVDTNGQTDLHNLYAIGEVSYTGLHGANRMASNSLLECVVYAW
AAAKDILKKHKQVDLASSLPSWDESQVTCSDEEVIIQHNWHELRLFMWDYMGIVRTDKRLERALRRIQMLQQETHEYYSN
FRVSNNLLELRNLLQVAELMVRCAMQRKESRGLHYTLDYPEQLENSGPTILRPEE

Sequences:

>Translated_535_residues
MNANREHECDVLVVGSGAAGLSLALRVANHCKVIVLSKGPRSEGATYYAQGGIAAVFDESDSIESHVQDTLIAGDGICEE
ATVKFIAENAKECVQWLIDGGVPFDREEDDSDDEPRYHLTREGGHSHRRILHAADATGMAMQTSLQDNAHNHPNIHVLER
HNALDLITEDKIGGDKNKVIGAYVWNRNQEHVETVKAKFVVLATGGASKVYQYTSNPDVSSGDGIAIAWRAGCRVANMEF
NQFHPTCLYHPEARNFLLTEALRGEGAYLRRPDGSRFMPDFDPREELAPRDVVARAIDFEMKRLGADCMYLDISHKPAEF
ITKHFPMIHSRLMDLGIDMTKEPIPIVPAAHYTCGGVMVDTNGQTDLHNLYAIGEVSYTGLHGANRMASNSLLECVVYAW
AAAKDILKKHKQVDLASSLPSWDESQVTCSDEEVIIQHNWHELRLFMWDYMGIVRTDKRLERALRRIQMLQQETHEYYSN
FRVSNNLLELRNLLQVAELMVRCAMQRKESRGLHYTLDYPEQLENSGPTILRPEE
>Mature_535_residues
MNANREHECDVLVVGSGAAGLSLALRVANHCKVIVLSKGPRSEGATYYAQGGIAAVFDESDSIESHVQDTLIAGDGICEE
ATVKFIAENAKECVQWLIDGGVPFDREEDDSDDEPRYHLTREGGHSHRRILHAADATGMAMQTSLQDNAHNHPNIHVLER
HNALDLITEDKIGGDKNKVIGAYVWNRNQEHVETVKAKFVVLATGGASKVYQYTSNPDVSSGDGIAIAWRAGCRVANMEF
NQFHPTCLYHPEARNFLLTEALRGEGAYLRRPDGSRFMPDFDPREELAPRDVVARAIDFEMKRLGADCMYLDISHKPAEF
ITKHFPMIHSRLMDLGIDMTKEPIPIVPAAHYTCGGVMVDTNGQTDLHNLYAIGEVSYTGLHGANRMASNSLLECVVYAW
AAAKDILKKHKQVDLASSLPSWDESQVTCSDEEVIIQHNWHELRLFMWDYMGIVRTDKRLERALRRIQMLQQETHEYYSN
FRVSNNLLELRNLLQVAELMVRCAMQRKESRGLHYTLDYPEQLENSGPTILRPEE

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=515, Percent_Identity=32.0388349514563, Blast_Score=204, Evalue=2e-52,
Organism=Escherichia coli, GI1788928, Length=534, Percent_Identity=74.7191011235955, Blast_Score=837, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=530, Percent_Identity=32.0754716981132, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI1786942, Length=558, Percent_Identity=31.1827956989247, Blast_Score=197, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17505833, Length=537, Percent_Identity=30.9124767225326, Blast_Score=213, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17550100, Length=538, Percent_Identity=31.7843866171004, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI71986328, Length=475, Percent_Identity=27.1578947368421, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322701, Length=556, Percent_Identity=30.7553956834532, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6322416, Length=571, Percent_Identity=31.3485113835377, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6320788, Length=489, Percent_Identity=26.1758691206544, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322511, Length=481, Percent_Identity=25.7796257796258, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17137288, Length=534, Percent_Identity=30.3370786516854, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24655642, Length=534, Percent_Identity=30.3370786516854, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24655647, Length=534, Percent_Identity=30.3370786516854, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24663005, Length=531, Percent_Identity=29.5668549905838, Blast_Score=197, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 60183; Mature: 60183

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNANREHECDVLVVGSGAAGLSLALRVANHCKVIVLSKGPRSEGATYYAQGGIAAVFDES
CCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCEEEEECCC
DSIESHVQDTLIAGDGICEEATVKFIAENAKECVQWLIDGGVPFDREEDDSDDEPRYHLT
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
REGGHSHRRILHAADATGMAMQTSLQDNAHNHPNIHVLERHNALDLITEDKIGGDKNKVI
CCCCCCHHHEEEECCCCCCEEEECHHCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCEE
GAYVWNRNQEHVETVKAKFVVLATGGASKVYQYTSNPDVSSGDGIAIAWRAGCRVANMEF
EEEEECCCHHHHHHHEEEEEEEEECCCCCEEEECCCCCCCCCCCEEEEEECCCEEECCCC
NQFHPTCLYHPEARNFLLTEALRGEGAYLRRPDGSRFMPDFDPREELAPRDVVARAIDFE
CCCCCEEEECCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHH
MKRLGADCMYLDISHKPAEFITKHFPMIHSRLMDLGIDMTKEPIPIVPAAHYTCGGVMVD
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEEE
TNGQTDLHNLYAIGEVSYTGLHGANRMASNSLLECVVYAWAAAKDILKKHKQVDLASSLP
CCCCCCHHHEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SWDESQVTCSDEEVIIQHNWHELRLFMWDYMGIVRTDKRLERALRRIQMLQQETHEYYSN
CCCCCCEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FRVSNNLLELRNLLQVAELMVRCAMQRKESRGLHYTLDYPEQLENSGPTILRPEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCEECCCC
>Mature Secondary Structure
MNANREHECDVLVVGSGAAGLSLALRVANHCKVIVLSKGPRSEGATYYAQGGIAAVFDES
CCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCEEEEECCC
DSIESHVQDTLIAGDGICEEATVKFIAENAKECVQWLIDGGVPFDREEDDSDDEPRYHLT
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
REGGHSHRRILHAADATGMAMQTSLQDNAHNHPNIHVLERHNALDLITEDKIGGDKNKVI
CCCCCCHHHEEEECCCCCCEEEECHHCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCEE
GAYVWNRNQEHVETVKAKFVVLATGGASKVYQYTSNPDVSSGDGIAIAWRAGCRVANMEF
EEEEECCCHHHHHHHEEEEEEEEECCCCCEEEECCCCCCCCCCCEEEEEECCCEEECCCC
NQFHPTCLYHPEARNFLLTEALRGEGAYLRRPDGSRFMPDFDPREELAPRDVVARAIDFE
CCCCCEEEECCCCCCCHHHHHHCCCCCEEECCCCCCCCCCCCCHHHCCHHHHHHHHHHHH
MKRLGADCMYLDISHKPAEFITKHFPMIHSRLMDLGIDMTKEPIPIVPAAHYTCGGVMVD
HHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCEEEE
TNGQTDLHNLYAIGEVSYTGLHGANRMASNSLLECVVYAWAAAKDILKKHKQVDLASSLP
CCCCCCHHHEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SWDESQVTCSDEEVIIQHNWHELRLFMWDYMGIVRTDKRLERALRRIQMLQQETHEYYSN
CCCCCCEEECCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FRVSNNLLELRNLLQVAELMVRCAMQRKESRGLHYTLDYPEQLENSGPTILRPEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]