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The map label for this gene is ygfZ [C]

Identifier: 27364925

GI number: 27364925

Start: 1527050

End: 1528024

Strand: Reverse

Name: ygfZ [C]

Synonym: VV1_1556

Alternate gene names: 27364925

Gene position: 1528024-1527050 (Counterclockwise)

Preceding gene: 27364927

Following gene: 27364924

Centisome position: 46.56

GC content: 51.08

Gene sequence:

>975_bases
ATGCAATCGACTCAACCAATCCAACGATGCGTACTTGGCAGCCAACAAGCGTTACCAGAGCTCGCCGTCAGCTTATTAGA
TAATCTTGGTCTGATTACCATGACAGGTAACGATAAAAAATCGTACCTTCAAGGTCAGGTGACTTGCGACGTCGTTTCTT
TAGAAACGGATCAAGTGACTTGGGGCGGCCACTGCGATGCAAAAGGCAAGCTGTGGAGTGTCTTCCGTCTGTTTCATTAC
GCCGACGGCTACGCAATGTTGCAAGATAAGTCAGCCATTGACGTGGAGTTGCGTGAACTCAAAAAATACGCCGTCTTTGC
CAAGGTTGAAATCAATGTTAGCGATGCCATCTTGCTGGGCGTTTGCGGTGTACAAGCGGAACAAGCCATCGCGAAACTGA
CCAACAATGCAGAGGCGGCAGTGGTAACCTTTGCCCAAGGGACAGCGGTAAAGATCTCACCACAGCGCTGGTTATTGGTC
GTAGATGCAAACCAACAAGATGAAGTGCTTGCCATGTTGGCGACAGCGCCGTTGTGTGACCACGCTCTTTGGGATCTTTA
CGATATCCTAGAAGTCGCTCCACGCATCCCTGCTTTTGCCCAAAATGAGCACATTCCTCAAGCGGTAAATTTGCAGGCGG
TGAATGGCATCAGCTTTAAAAAGGGCTGCTACACAGGCCAAGAGACCGTTGCACGAGCCAAATATCGTGGCATCAACAAG
CGAGCTCTGTATCGACTCTCTGGCACCATAGCACCATCAGCACCAGAGACAACGATCAGCTTGGAACGCAGTGTGGGCGA
TAACTGGCGCGCGGCTGGTGAAGCGTTGGTGAGTTATCACTTTGATGATGGGCGCGCGACCGGACTCTTTGTGCTACCAA
ATGATCTGGAGCCCGAGACGCAATTTCGCCTAGCAGGTCAAAGTGAGCAATTGTGGCAACGAGAGCCCCTACCGTATTCC
CTCGATGACGAATAG

Upstream 100 bases:

>100_bases
GCATCTCAACAAAGGGAAGCCCCTACATTTCCTCTGCTGACAAATGGTCACTTGCAGATTAACATACCTCAATAAATCGA
CTTTATGGATAGCGAATAAC

Downstream 100 bases:

>100_bases
TGATTTGTCCACAGCATTGACCCGCTATCTTGATGAGCAACAGGTGCCCTATCGTCTGTTGCCTCATCAAACACCAGCAA
CCACCATCGAAGATGCAGCC

Product: putative aminomethyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MQSTQPIQRCVLGSQQALPELAVSLLDNLGLITMTGNDKKSYLQGQVTCDVVSLETDQVTWGGHCDAKGKLWSVFRLFHY
ADGYAMLQDKSAIDVELRELKKYAVFAKVEINVSDAILLGVCGVQAEQAIAKLTNNAEAAVVTFAQGTAVKISPQRWLLV
VDANQQDEVLAMLATAPLCDHALWDLYDILEVAPRIPAFAQNEHIPQAVNLQAVNGISFKKGCYTGQETVARAKYRGINK
RALYRLSGTIAPSAPETTISLERSVGDNWRAAGEALVSYHFDDGRATGLFVLPNDLEPETQFRLAGQSEQLWQREPLPYS
LDDE

Sequences:

>Translated_324_residues
MQSTQPIQRCVLGSQQALPELAVSLLDNLGLITMTGNDKKSYLQGQVTCDVVSLETDQVTWGGHCDAKGKLWSVFRLFHY
ADGYAMLQDKSAIDVELRELKKYAVFAKVEINVSDAILLGVCGVQAEQAIAKLTNNAEAAVVTFAQGTAVKISPQRWLLV
VDANQQDEVLAMLATAPLCDHALWDLYDILEVAPRIPAFAQNEHIPQAVNLQAVNGISFKKGCYTGQETVARAKYRGINK
RALYRLSGTIAPSAPETTISLERSVGDNWRAAGEALVSYHFDDGRATGLFVLPNDLEPETQFRLAGQSEQLWQREPLPYS
LDDE
>Mature_324_residues
MQSTQPIQRCVLGSQQALPELAVSLLDNLGLITMTGNDKKSYLQGQVTCDVVSLETDQVTWGGHCDAKGKLWSVFRLFHY
ADGYAMLQDKSAIDVELRELKKYAVFAKVEINVSDAILLGVCGVQAEQAIAKLTNNAEAAVVTFAQGTAVKISPQRWLLV
VDANQQDEVLAMLATAPLCDHALWDLYDILEVAPRIPAFAQNEHIPQAVNLQAVNGISFKKGCYTGQETVARAKYRGINK
RALYRLSGTIAPSAPETTISLERSVGDNWRAAGEALVSYHFDDGRATGLFVLPNDLEPETQFRLAGQSEQLWQREPLPYS
LDDE

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family

Homologues:

Organism=Escherichia coli, GI1789265, Length=310, Percent_Identity=43.8709677419355, Blast_Score=226, Evalue=2e-60,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): YGFZ_VIBVU (Q8DC85)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_760453.1
- HSSP:   P0ADE8
- ProteinModelPortal:   Q8DC85
- SMR:   Q8DC85
- GeneID:   1178469
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_1556
- HOGENOM:   HBG700628
- OMA:   GAHCDPK
- ProtClustDB:   CLSK874810
- BioCyc:   VVUL216895:VV1_1556-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01175
- InterPro:   IPR017703
- InterPro:   IPR006222
- TIGRFAMs:   TIGR03317

Pfam domain/function: PF01571 GCV_T

EC number: NA

Molecular weight: Translated: 35612; Mature: 35612

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: BINDING 184-184

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSTQPIQRCVLGSQQALPELAVSLLDNLGLITMTGNDKKSYLQGQVTCDVVSLETDQVT
CCCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCEEEEEEEEEECCCEE
WGGHCDAKGKLWSVFRLFHYADGYAMLQDKSAIDVELRELKKYAVFAKVEINVSDAILLG
ECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHEEEEEEEEECCCCEEEEE
VCGVQAEQAIAKLTNNAEAAVVTFAQGTAVKISPQRWLLVVDANQQDEVLAMLATAPLCD
ECCCCHHHHHHHHCCCCCEEEEEEECCCEEEECCCEEEEEEECCCCHHHHHHHHHCCHHH
HALWDLYDILEVAPRIPAFAQNEHIPQAVNLQAVNGISFKKGCYTGQETVARAKYRGINK
HHHHHHHHHHHHHCCCCCCCCCCCCCCEECEEEECCCCCCCCCCCCHHHHHHHHHCCCCH
RALYRLSGTIAPSAPETTISLERSVGDNWRAAGEALVSYHFDDGRATGLFVLPNDLEPET
HEEEEEECEECCCCCCCEEEEECCCCCCHHHHCCEEEEEEECCCCEEEEEEECCCCCCCC
QFRLAGQSEQLWQREPLPYSLDDE
EEEECCCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQSTQPIQRCVLGSQQALPELAVSLLDNLGLITMTGNDKKSYLQGQVTCDVVSLETDQVT
CCCCHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCEEEEEEEEEECCCEE
WGGHCDAKGKLWSVFRLFHYADGYAMLQDKSAIDVELRELKKYAVFAKVEINVSDAILLG
ECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHEEEEEEEEECCCCEEEEE
VCGVQAEQAIAKLTNNAEAAVVTFAQGTAVKISPQRWLLVVDANQQDEVLAMLATAPLCD
ECCCCHHHHHHHHCCCCCEEEEEEECCCEEEECCCEEEEEEECCCCHHHHHHHHHCCHHH
HALWDLYDILEVAPRIPAFAQNEHIPQAVNLQAVNGISFKKGCYTGQETVARAKYRGINK
HHHHHHHHHHHHHCCCCCCCCCCCCCCEECEEEECCCCCCCCCCCCHHHHHHHHHCCCCH
RALYRLSGTIAPSAPETTISLERSVGDNWRAAGEALVSYHFDDGRATGLFVLPNDLEPET
HEEEEEECEECCCCCCCEEEEECCCCCCHHHHCCEEEEEEECCCCEEEEEEECCCCCCCC
QFRLAGQSEQLWQREPLPYSLDDE
EEEECCCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA