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The map label for this gene is purD [H]

Identifier: 27364629

GI number: 27364629

Start: 1205003

End: 1206292

Strand: Reverse

Name: purD [H]

Synonym: VV1_1226

Alternate gene names: 27364629

Gene position: 1206292-1205003 (Counterclockwise)

Preceding gene: 27364630

Following gene: 27364626

Centisome position: 36.76

GC content: 53.72

Gene sequence:

>1290_bases
ATGAATGTATTGATAATTGGTTCCGGCGGTCGTGAACACGCTCTAGGTTGGAAAGCCGCGCAAAACCCCAATGTGGAGAC
AATCTTCGTCGCGCCGGGTAACGCTGGTACCGCACTTGAGCCTAAGCTAGAAAACGTCAACATCGCCGTTGAAGACATTG
CCGGTTTAGTGGCGTTTGCTAAAGAGAAAGCCATTGAACTGACCATCGTCGGTCCTGAAGTGCCACTCGTACTGGGTGTC
GTTGATGCGTTTTATGAAGCAGGCCTGCCAATTTTTGGCCCTACCCAAGCGGCTGCGCAACTTGAAGGCTCTAAAGCATT
TACCAAAGACTTCCTTGCTCGTCACCAAATCCCGACGGCGGCGTACGCAAACTTTACGGACATCGAGCCAGCACTGGCCT
ATGTACGTGAACAAGGCGCGCCAATCGTGGTGAAAGCAGATGGCCTTGCGGCAGGTAAAGGCGTGATTGTCGCGATGACG
CTGGAAGAAGCGGAAGAAGCAATCAAAGACATGCTCGCAGGCAACGCCTTTGGCGAGGCAGGCAGCCGCGTGGTGATCGA
AGAGTTCCTTGATGGCGAAGAAGCGAGCTTCATCGTGATGGTGGATGGCGAAAACGTACTGCCAATGGCCACCAGCCAAG
ACCACAAGCGTGTCGGCGATAAAGACACCGGTCCAAACACCGGCGGTATGGGCGCTTACTCACCCGCACCAGTGGTGACA
CCAGAGATCCATAACCGTGTTATGCAAGAGGTGATCTTCCCTACCGTGCGTGGCATGGCAGCCGAAGGTAACCCATACAC
GGGCTTCCTCTATGCCGGCTTGATGATTGACAAAGATGGCACACCAAAAGTGATCGAGTACAACTGCCGCTTTGGTGACC
CAGAAACGCAACCTATCATGATGCGCATGGAATCGGATCTTGTGGAATTGTGCCTCGCGGCCATCGACAAGAAACTGGAT
CAAGTCGAGTCCAAGTGGGACCCTCGCGCATCGATCGGTATTGTGCTTGCAGCGGGTGGCTATCCAGCGGCGTACAACAA
GGGCGACGTGATCTCTGGCCTACCTCAAGTTGAAATTGAAGGCGAAAAAGTCTTCCACGCTGGCACTGAAAATAAAGAGG
GCGATATCGTAACAAACGGTGGTCGTGTTCTTTGTGCCACCGCACTGGGCAACAGCGTATCAGAAGCGCAGCAACGCGCG
TACGAGCTGGCAAAACAGATCCGTTGGGAAGGCATGTTCCACCGCAACGATATTGGCTACCGTGCTATCGCTCGCGAGCA
ACAAAAATAA

Upstream 100 bases:

>100_bases
TATGGCGATGATTTTTACGGGCATGCGCCACTTCCGCCACTAGTTTTTATCAGAGGTGCCTTATCAGGCACCTCTTTTTG
CAAGATTTGAGGATGATGAG

Downstream 100 bases:

>100_bases
CAACAACATCAGTTGGCAAAGAAAACAAAAGGCGCATTTTGCGCCTTTTGTCTTTTCATTCGTCTAACGGAGTAAGTCTT
ACTCTTCAAGCAACACGGGC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNVLIIGSGGREHALGWKAAQNPNVETIFVAPGNAGTALEPKLENVNIAVEDIAGLVAFAKEKAIELTIVGPEVPLVLGV
VDAFYEAGLPIFGPTQAAAQLEGSKAFTKDFLARHQIPTAAYANFTDIEPALAYVREQGAPIVVKADGLAAGKGVIVAMT
LEEAEEAIKDMLAGNAFGEAGSRVVIEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
PEIHNRVMQEVIFPTVRGMAAEGNPYTGFLYAGLMIDKDGTPKVIEYNCRFGDPETQPIMMRMESDLVELCLAAIDKKLD
QVESKWDPRASIGIVLAAGGYPAAYNKGDVISGLPQVEIEGEKVFHAGTENKEGDIVTNGGRVLCATALGNSVSEAQQRA
YELAKQIRWEGMFHRNDIGYRAIAREQQK

Sequences:

>Translated_429_residues
MNVLIIGSGGREHALGWKAAQNPNVETIFVAPGNAGTALEPKLENVNIAVEDIAGLVAFAKEKAIELTIVGPEVPLVLGV
VDAFYEAGLPIFGPTQAAAQLEGSKAFTKDFLARHQIPTAAYANFTDIEPALAYVREQGAPIVVKADGLAAGKGVIVAMT
LEEAEEAIKDMLAGNAFGEAGSRVVIEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
PEIHNRVMQEVIFPTVRGMAAEGNPYTGFLYAGLMIDKDGTPKVIEYNCRFGDPETQPIMMRMESDLVELCLAAIDKKLD
QVESKWDPRASIGIVLAAGGYPAAYNKGDVISGLPQVEIEGEKVFHAGTENKEGDIVTNGGRVLCATALGNSVSEAQQRA
YELAKQIRWEGMFHRNDIGYRAIAREQQK
>Mature_429_residues
MNVLIIGSGGREHALGWKAAQNPNVETIFVAPGNAGTALEPKLENVNIAVEDIAGLVAFAKEKAIELTIVGPEVPLVLGV
VDAFYEAGLPIFGPTQAAAQLEGSKAFTKDFLARHQIPTAAYANFTDIEPALAYVREQGAPIVVKADGLAAGKGVIVAMT
LEEAEEAIKDMLAGNAFGEAGSRVVIEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
PEIHNRVMQEVIFPTVRGMAAEGNPYTGFLYAGLMIDKDGTPKVIEYNCRFGDPETQPIMMRMESDLVELCLAAIDKKLD
QVESKWDPRASIGIVLAAGGYPAAYNKGDVISGLPQVEIEGEKVFHAGTENKEGDIVTNGGRVLCATALGNSVSEAQQRA
YELAKQIRWEGMFHRNDIGYRAIAREQQK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=429, Percent_Identity=49.6503496503497, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI209869995, Length=429, Percent_Identity=49.6503496503497, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI209869993, Length=429, Percent_Identity=49.6503496503497, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI28416899, Length=429, Percent_Identity=49.6503496503497, Blast_Score=412, Evalue=1e-115,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=75.0582750582751, Blast_Score=667, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=41.8823529411765, Blast_Score=316, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=42.6966292134831, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=45.5813953488372, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=45.5813953488372, Blast_Score=353, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45831; Mature: 45831

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLIIGSGGREHALGWKAAQNPNVETIFVAPGNAGTALEPKLENVNIAVEDIAGLVAFA
CEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHH
KEKAIELTIVGPEVPLVLGVVDAFYEAGLPIFGPTQAAAQLEGSKAFTKDFLARHQIPTA
HCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCH
AYANFTDIEPALAYVREQGAPIVVKADGLAAGKGVIVAMTLEEAEEAIKDMLAGNAFGEA
HCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVIEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
CCEEEHHHHCCCCCCEEEEEECCCCEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PEIHNRVMQEVIFPTVRGMAAEGNPYTGFLYAGLMIDKDGTPKVIEYNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCEE
MRMESDLVELCLAAIDKKLDQVESKWDPRASIGIVLAAGGYPAAYNKGDVISGLPQVEIE
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHCCCCCEEEC
GEKVFHAGTENKEGDIVTNGGRVLCATALGNSVSEAQQRAYELAKQIRWEGMFHRNDIGY
CCEEEECCCCCCCCCEEECCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCCH
RAIAREQQK
HHHHHCCCC
>Mature Secondary Structure
MNVLIIGSGGREHALGWKAAQNPNVETIFVAPGNAGTALEPKLENVNIAVEDIAGLVAFA
CEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHH
KEKAIELTIVGPEVPLVLGVVDAFYEAGLPIFGPTQAAAQLEGSKAFTKDFLARHQIPTA
HCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCH
AYANFTDIEPALAYVREQGAPIVVKADGLAAGKGVIVAMTLEEAEEAIKDMLAGNAFGEA
HCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVIEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDKDTGPNTGGMGAYSPAPVVT
CCEEEHHHHCCCCCCEEEEEECCCCEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PEIHNRVMQEVIFPTVRGMAAEGNPYTGFLYAGLMIDKDGTPKVIEYNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCEE
MRMESDLVELCLAAIDKKLDQVESKWDPRASIGIVLAAGGYPAAYNKGDVISGLPQVEIE
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHCCCCCEEEC
GEKVFHAGTENKEGDIVTNGGRVLCATALGNSVSEAQQRAYELAKQIRWEGMFHRNDIGY
CCEEEECCCCCCCCCEEECCCCEEEEEHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCCH
RAIAREQQK
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]