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The map label for this gene is sthA [H]

Identifier: 27364584

GI number: 27364584

Start: 1161670

End: 1163070

Strand: Reverse

Name: sthA [H]

Synonym: VV1_1168

Alternate gene names: 27364584

Gene position: 1163070-1161670 (Counterclockwise)

Preceding gene: 27364587

Following gene: 27364581

Centisome position: 35.44

GC content: 50.32

Gene sequence:

>1401_bases
ATGGCGCATGCGAATCACTTCGATGTGATTGTTATCGGGAGCGGCCCTGGCGGTGAAGGGGCAGCAATGGGTTTAACCAA
AGCAGGCCTGAAAGTTGCCGTGGTGGAAAAAGAGAGCAGTGTTGGCGGCGGCTGTACTCACTGGGGCACCATTCCATCCA
AAGCGCTGCGCCATGCGGTTAGCCGTATTATTGAATTCAACAGCAACCCGCTGTTTTGCAAAAACAACTCCAGTTTACAC
GCCACATTTTCAACCATTCTCGGCCATGCGAAATCCGTTATCGACAAGCAGACTCGCTTACGCCAAGGCTTTTATGATCG
CAATCAGTGCCAATTGATTTTCGGAACCGCCCGTTTTACCGATGCGCACACCATTAGCGTGACGCAAAACGACGGCACTG
AAGAAGTGTATACGGCGGATAAGTTTGTGATTGCTACTGGCTCTCGGCCTTATCAACCAGCCGATGTCGACTTTAATCAT
GAGCGAATCTACGACAGCGATTCCATTCTCAGTCTAAAACACGACCCTCGCCACATCATCATTTATGGCGCAGGGGTGAT
TGGTTGTGAATACGCATCCATCTTCCGTGGTTTGGGGGTGAAAACCGACCTCATCAACACACGCGATCGCTTACTGGCCT
TTTTGGACAACGAAGTTTCCGATGCACTTTCCTATCACTTCTGGAACAGCGGCGTCGTGATCCGTAATGACGAGACCTAT
GAACGCATTGAAGGCACCGAAGATGGGGTGATTGTTCATCTTCAATCGGGCAAGAAGATGAAAGCCGATTGCTTGCTTTA
CGCCAATGGCCGTACTGGTAACACTGACAAGTTGAACCTGCCTGCAGTTGGGCTTCAAGGTGACTCTCGCGGCCAATTGA
AAGTCGATGGCAATTACCAAACCGAAGTGGAACACGTTTACGCGGTAGGCGATGTGATTGGTTACCCAAGTCTTGCGAGC
GCGGCATATGACCAAGGGCGCTTTGTAGCGCAAGCCATCACTAAGGGCAAAGCCGATGGCTATCTGATTGATGACATCCC
TACGGGGATTTACACCATTCCGGAAATCAGCTCGGTTGGTAAAACAGAACAAGAATTAACCGCGGCGAAAGTACCCTATG
AAGTTGGCCGCTCTTCGTTCAAACATCTGGCTCGTGCGCAGATTGCCGGCAAAGACATCGGCAGCTTGAAGATCCTCTTC
CATCGTGAAACCAAAGAAATTCTTGGTATTCACTGCTTTGGGGAACGCGCGGCGGAAATCATTCACATTGGCCAAGCGAT
CATGGAGCAAAAAGGCGAAGCCAATACCATCGAGTATTTCGTCAATACTACCTTCAACTACCCCACCATGGCCGAAGCGT
ATCGTGTTGCTGCACTCAATGGACTCAACCGTCTGTTCTAG

Upstream 100 bases:

>100_bases
ATCACTTCCACTCTCTACCGAGCCTGCTGTTTACCTGCTGGTATAATCAGTTAAAATCTCGCTAAAGCAATGTTAAGCAA
ATATAACAAGGAATGAAATC

Downstream 100 bases:

>100_bases
TGTGAAAACACCCACTTCCCAAACAAAAACAGCAGGAATTCCTGCTGTTTTTTTTACCATTTCATATGGACCACGACGGG
AGTAAGACGATGTTTCAGCA

Product: soluble pyridine nucleotide transhydrogenase

Products: NA

Alternate protein names: STH; NAD(P)(+) transhydrogenase [B-specific] [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MAHANHFDVIVIGSGPGGEGAAMGLTKAGLKVAVVEKESSVGGGCTHWGTIPSKALRHAVSRIIEFNSNPLFCKNNSSLH
ATFSTILGHAKSVIDKQTRLRQGFYDRNQCQLIFGTARFTDAHTISVTQNDGTEEVYTADKFVIATGSRPYQPADVDFNH
ERIYDSDSILSLKHDPRHIIIYGAGVIGCEYASIFRGLGVKTDLINTRDRLLAFLDNEVSDALSYHFWNSGVVIRNDETY
ERIEGTEDGVIVHLQSGKKMKADCLLYANGRTGNTDKLNLPAVGLQGDSRGQLKVDGNYQTEVEHVYAVGDVIGYPSLAS
AAYDQGRFVAQAITKGKADGYLIDDIPTGIYTIPEISSVGKTEQELTAAKVPYEVGRSSFKHLARAQIAGKDIGSLKILF
HRETKEILGIHCFGERAAEIIHIGQAIMEQKGEANTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF

Sequences:

>Translated_466_residues
MAHANHFDVIVIGSGPGGEGAAMGLTKAGLKVAVVEKESSVGGGCTHWGTIPSKALRHAVSRIIEFNSNPLFCKNNSSLH
ATFSTILGHAKSVIDKQTRLRQGFYDRNQCQLIFGTARFTDAHTISVTQNDGTEEVYTADKFVIATGSRPYQPADVDFNH
ERIYDSDSILSLKHDPRHIIIYGAGVIGCEYASIFRGLGVKTDLINTRDRLLAFLDNEVSDALSYHFWNSGVVIRNDETY
ERIEGTEDGVIVHLQSGKKMKADCLLYANGRTGNTDKLNLPAVGLQGDSRGQLKVDGNYQTEVEHVYAVGDVIGYPSLAS
AAYDQGRFVAQAITKGKADGYLIDDIPTGIYTIPEISSVGKTEQELTAAKVPYEVGRSSFKHLARAQIAGKDIGSLKILF
HRETKEILGIHCFGERAAEIIHIGQAIMEQKGEANTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF
>Mature_465_residues
AHANHFDVIVIGSGPGGEGAAMGLTKAGLKVAVVEKESSVGGGCTHWGTIPSKALRHAVSRIIEFNSNPLFCKNNSSLHA
TFSTILGHAKSVIDKQTRLRQGFYDRNQCQLIFGTARFTDAHTISVTQNDGTEEVYTADKFVIATGSRPYQPADVDFNHE
RIYDSDSILSLKHDPRHIIIYGAGVIGCEYASIFRGLGVKTDLINTRDRLLAFLDNEVSDALSYHFWNSGVVIRNDETYE
RIEGTEDGVIVHLQSGKKMKADCLLYANGRTGNTDKLNLPAVGLQGDSRGQLKVDGNYQTEVEHVYAVGDVIGYPSLASA
AYDQGRFVAQAITKGKADGYLIDDIPTGIYTIPEISSVGKTEQELTAAKVPYEVGRSSFKHLARAQIAGKDIGSLKILFH
RETKEILGIHCFGERAAEIIHIGQAIMEQKGEANTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF

Specific function: Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=473, Percent_Identity=28.9640591966173, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI50301238, Length=463, Percent_Identity=28.5097192224622, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI22035672, Length=423, Percent_Identity=28.8416075650118, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI33519430, Length=401, Percent_Identity=24.1895261845387, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI33519428, Length=401, Percent_Identity=24.1895261845387, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI33519426, Length=401, Percent_Identity=24.1895261845387, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI148277065, Length=403, Percent_Identity=24.0694789081886, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI148277071, Length=321, Percent_Identity=25.5451713395639, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI291045266, Length=435, Percent_Identity=24.367816091954, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI291045268, Length=309, Percent_Identity=25.8899676375405, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI87082354, Length=466, Percent_Identity=75.9656652360515, Blast_Score=754, Evalue=0.0,
Organism=Escherichia coli, GI1786307, Length=467, Percent_Identity=29.7644539614561, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789915, Length=434, Percent_Identity=29.7235023041475, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI87081717, Length=466, Percent_Identity=26.8240343347639, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI32565766, Length=472, Percent_Identity=30.2966101694915, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17557007, Length=472, Percent_Identity=24.7881355932203, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=429, Percent_Identity=27.5058275058275, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71983429, Length=429, Percent_Identity=27.5058275058275, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71982272, Length=491, Percent_Identity=24.6435845213849, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17559934, Length=257, Percent_Identity=24.5136186770428, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=28.2157676348548, Blast_Score=169, Evalue=7e-43,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=27.7542372881356, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6325166, Length=469, Percent_Identity=27.7185501066098, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21358499, Length=460, Percent_Identity=31.304347826087, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24640553, Length=484, Percent_Identity=28.099173553719, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24640551, Length=481, Percent_Identity=27.8586278586279, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24640549, Length=484, Percent_Identity=28.099173553719, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI17737741, Length=487, Percent_Identity=26.0780287474333, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327
- InterPro:   IPR022962 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.6.1.1 [H]

Molecular weight: Translated: 50956; Mature: 50825

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHANHFDVIVIGSGPGGEGAAMGLTKAGLKVAVVEKESSVGGGCTHWGTIPSKALRHAV
CCCCCEEEEEEEECCCCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
SRIIEFNSNPLFCKNNSSLHATFSTILGHAKSVIDKQTRLRQGFYDRNQCQLIFGTARFT
HHHHHCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEC
DAHTISVTQNDGTEEVYTADKFVIATGSRPYQPADVDFNHERIYDSDSILSLKHDPRHII
CCEEEEEECCCCCCEEEECCEEEEEECCCCCCCCCCCCCHHEECCCCCEEEEECCCCEEE
IYGAGVIGCEYASIFRGLGVKTDLINTRDRLLAFLDNEVSDALSYHFWNSGVVIRNDETY
EEECCCHHHHHHHHHHCCCCEEHHCCCHHHHHHHHHHHHHHHHHEEEECCCEEEECCCHH
ERIEGTEDGVIVHLQSGKKMKADCLLYANGRTGNTDKLNLPAVGLQGDSRGQLKVDGNYQ
HHHCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCEEECCEEECCCCCCCEEEECCCCC
TEVEHVYAVGDVIGYPSLASAAYDQGRFVAQAITKGKADGYLIDDIPTGIYTIPEISSVG
CCHHEEEEEHHHHCCCCHHHHHHHCCHHHHHHHHCCCCCCEEEECCCCCEEECCCCHHCC
KTEQELTAAKVPYEVGRSSFKHLARAQIAGKDIGSLKILFHRETKEILGIHCFGERAAEI
CCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHEEEEEECHHHHHH
IHIGQAIMEQKGEANTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF
HHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AHANHFDVIVIGSGPGGEGAAMGLTKAGLKVAVVEKESSVGGGCTHWGTIPSKALRHAV
CCCCEEEEEEEECCCCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
SRIIEFNSNPLFCKNNSSLHATFSTILGHAKSVIDKQTRLRQGFYDRNQCQLIFGTARFT
HHHHHCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEC
DAHTISVTQNDGTEEVYTADKFVIATGSRPYQPADVDFNHERIYDSDSILSLKHDPRHII
CCEEEEEECCCCCCEEEECCEEEEEECCCCCCCCCCCCCHHEECCCCCEEEEECCCCEEE
IYGAGVIGCEYASIFRGLGVKTDLINTRDRLLAFLDNEVSDALSYHFWNSGVVIRNDETY
EEECCCHHHHHHHHHHCCCCEEHHCCCHHHHHHHHHHHHHHHHHEEEECCCEEEECCCHH
ERIEGTEDGVIVHLQSGKKMKADCLLYANGRTGNTDKLNLPAVGLQGDSRGQLKVDGNYQ
HHHCCCCCCEEEEECCCCCEEEEEEEEECCCCCCCCEEECCEEECCCCCCCEEEECCCCC
TEVEHVYAVGDVIGYPSLASAAYDQGRFVAQAITKGKADGYLIDDIPTGIYTIPEISSVG
CCHHEEEEEHHHHCCCCHHHHHHHCCHHHHHHHHCCCCCCEEEECCCCCEEECCCCHHCC
KTEQELTAAKVPYEVGRSSFKHLARAQIAGKDIGSLKILFHRETKEILGIHCFGERAAEI
CCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHEEEEEECHHHHHH
IHIGQAIMEQKGEANTIEYFVNTTFNYPTMAEAYRVAALNGLNRLF
HHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]