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The map label for this gene is trmA [H]

Identifier: 27364587

GI number: 27364587

Start: 1164434

End: 1165543

Strand: Reverse

Name: trmA [H]

Synonym: VV1_1171

Alternate gene names: 27364587

Gene position: 1165543-1164434 (Counterclockwise)

Preceding gene: 27364588

Following gene: 27364584

Centisome position: 35.51

GC content: 47.3

Gene sequence:

>1110_bases
ATGGCGACTCTTGATGTAAACCCACAACGCTATCAGCAACAGTTAGCAGAAAAAGTGCAACGCTTAACCGATATGTTCGC
ACCTTATCAAGCCCCTGAGCTGGAAGTGTTTGAGTCTCCAGATCAGCACTATCGCATGCGCGCTGAGTTCCGCGTTTGGC
ATGAAGGGGAAGAGATGTACTACATCATGTTCAACCAAGAGACGCGTGAAAAATACCGTGTTGACCAGTTTCCGGCAGCA
AGCCGCTTGATCAACGATCTCATGCCTTTGCTGATTGACGCGATGAAAGACAATGAGTCGCTACGTCGTAAACTGTTCCA
AGTCGATTTCCTTTCCACTCTGAGCGGCGAGATTCTGGTTTCTTTGCTTTACCATCGCCAATTGGATGATGCTTGGATTG
AAAATGCTAAAGCTTTAAAACAGCGCCTGAATGACGAAGGGTTTAACCTCAACCTCATTGGTCGCGCACGTAAAATGAAG
ATCGTCCTAGACCGTGATTACGTGGTCGAAAAGCTCGACGTCAACGGCAAGCCTTACACCTACCAACAAGTAGAAAACAG
CTTTACTCAACCGAACGGCAAAGTCGCAGAGAAAATGTTGGAATGGGCGGTCGATTGTACCCAAGAAAGCCAAGGCGATT
TATTGGAGCTTTACTGTGGTAACGGCAATTTCTCCCTTGCGCTGGCGCAAAATTTTGAGCGCGTGTTGGCCACGGAATTG
GCAAAGCCATCGGTGGAATCGGCGCAATACAATATCGCTGCGAACAAGATTGAGAATGTGCAAATCATCCGCATGTCAGC
TGAAGAGTTTACCGAAGCAATGGAAGGGAAGCGTGAATTCCGTCGCCTGAAAGACAATGGCATCGATCTCAAGAGTTATA
ACTGCAACACCATTTTTGTTGATCCACCACGCTCTGGCATGGATGTAGACACTTGTAAAATGGTGCAAGGTTACGAGCGT
ATTTTATACATCTCTTGCAACCCAGAGACATTAAAAGAGAACTTAGACATTCTGAGCGAAACACACCACATCACGCGCTT
TGCTCTGTTCGATCAGTTCCCGTACACCCACCACATGGAAGCGGGTGTGATGCTAGAGCGTAAAGCCTAA

Upstream 100 bases:

>100_bases
ACAGTAGTACTTATCAATTTCCCCTTGTCACTGGACAATGAACGGGGATTGAATAAAATCTGCGCTCTTTATCTCTAAAG
CACCCGAAAGGTATTAATTC

Downstream 100 bases:

>100_bases
GCAGTATCAAACAAAAACGAGCCGCGAATGGCTCGTTTTTTTCTCTCTAAAATCGTACTAATCGGCACTTGGGCGACGAA
TGTATCCCAGCTTACTGCCA

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MATLDVNPQRYQQQLAEKVQRLTDMFAPYQAPELEVFESPDQHYRMRAEFRVWHEGEEMYYIMFNQETREKYRVDQFPAA
SRLINDLMPLLIDAMKDNESLRRKLFQVDFLSTLSGEILVSLLYHRQLDDAWIENAKALKQRLNDEGFNLNLIGRARKMK
IVLDRDYVVEKLDVNGKPYTYQQVENSFTQPNGKVAEKMLEWAVDCTQESQGDLLELYCGNGNFSLALAQNFERVLATEL
AKPSVESAQYNIAANKIENVQIIRMSAEEFTEAMEGKREFRRLKDNGIDLKSYNCNTIFVDPPRSGMDVDTCKMVQGYER
ILYISCNPETLKENLDILSETHHITRFALFDQFPYTHHMEAGVMLERKA

Sequences:

>Translated_369_residues
MATLDVNPQRYQQQLAEKVQRLTDMFAPYQAPELEVFESPDQHYRMRAEFRVWHEGEEMYYIMFNQETREKYRVDQFPAA
SRLINDLMPLLIDAMKDNESLRRKLFQVDFLSTLSGEILVSLLYHRQLDDAWIENAKALKQRLNDEGFNLNLIGRARKMK
IVLDRDYVVEKLDVNGKPYTYQQVENSFTQPNGKVAEKMLEWAVDCTQESQGDLLELYCGNGNFSLALAQNFERVLATEL
AKPSVESAQYNIAANKIENVQIIRMSAEEFTEAMEGKREFRRLKDNGIDLKSYNCNTIFVDPPRSGMDVDTCKMVQGYER
ILYISCNPETLKENLDILSETHHITRFALFDQFPYTHHMEAGVMLERKA
>Mature_368_residues
ATLDVNPQRYQQQLAEKVQRLTDMFAPYQAPELEVFESPDQHYRMRAEFRVWHEGEEMYYIMFNQETREKYRVDQFPAAS
RLINDLMPLLIDAMKDNESLRRKLFQVDFLSTLSGEILVSLLYHRQLDDAWIENAKALKQRLNDEGFNLNLIGRARKMKI
VLDRDYVVEKLDVNGKPYTYQQVENSFTQPNGKVAEKMLEWAVDCTQESQGDLLELYCGNGNFSLALAQNFERVLATELA
KPSVESAQYNIAANKIENVQIIRMSAEEFTEAMEGKREFRRLKDNGIDLKSYNCNTIFVDPPRSGMDVDTCKMVQGYERI
LYISCNPETLKENLDILSETHHITRFALFDQFPYTHHMEAGVMLERKA

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=201, Percent_Identity=26.865671641791, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI51173878, Length=201, Percent_Identity=26.865671641791, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1790403, Length=360, Percent_Identity=64.4444444444444, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1789148, Length=300, Percent_Identity=27.6666666666667, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71997438, Length=176, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24666579, Length=194, Percent_Identity=25.7731958762887, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011869
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.35 [H]

Molecular weight: Translated: 43066; Mature: 42934

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLDVNPQRYQQQLAEKVQRLTDMFAPYQAPELEVFESPDQHYRMRAEFRVWHEGEEMY
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHEEEEEEEEEECCCEEE
YIMFNQETREKYRVDQFPAASRLINDLMPLLIDAMKDNESLRRKLFQVDFLSTLSGEILV
EEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SLLYHRQLDDAWIENAKALKQRLNDEGFNLNLIGRARKMKIVLDRDYVVEKLDVNGKPYT
HHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEECCCCEEHEECCCCCCEE
YQQVENSFTQPNGKVAEKMLEWAVDCTQESQGDLLELYCGNGNFSLALAQNFERVLATEL
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHH
AKPSVESAQYNIAANKIENVQIIRMSAEEFTEAMEGKREFRRLKDNGIDLKSYNCNTIFV
CCCCCCCCEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEE
DPPRSGMDVDTCKMVQGYERILYISCNPETLKENLDILSETHHITRFALFDQFPYTHHME
CCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AGVMLERKA
CCEEEECCC
>Mature Secondary Structure 
ATLDVNPQRYQQQLAEKVQRLTDMFAPYQAPELEVFESPDQHYRMRAEFRVWHEGEEMY
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHEEEEEEEEEECCCEEE
YIMFNQETREKYRVDQFPAASRLINDLMPLLIDAMKDNESLRRKLFQVDFLSTLSGEILV
EEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SLLYHRQLDDAWIENAKALKQRLNDEGFNLNLIGRARKMKIVLDRDYVVEKLDVNGKPYT
HHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEECCCCEEHEECCCCCCEE
YQQVENSFTQPNGKVAEKMLEWAVDCTQESQGDLLELYCGNGNFSLALAQNFERVLATEL
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHH
AKPSVESAQYNIAANKIENVQIIRMSAEEFTEAMEGKREFRRLKDNGIDLKSYNCNTIFV
CCCCCCCCEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEE
DPPRSGMDVDTCKMVQGYERILYISCNPETLKENLDILSETHHITRFALFDQFPYTHHME
CCCCCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AGVMLERKA
CCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA