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The map label for this gene is dnaN [H]

Identifier: 27364432

GI number: 27364432

Start: 1002664

End: 1003764

Strand: Reverse

Name: dnaN [H]

Synonym: VV1_0998

Alternate gene names: 27364432

Gene position: 1003764-1002664 (Counterclockwise)

Preceding gene: 326423789

Following gene: 27364431

Centisome position: 30.59

GC content: 50.32

Gene sequence:

>1101_bases
ATGAAATTTACTATCGCACGTAGTCATTTTATTAAACCCCTTCAACAGGTTTCCGGTGCACTCGGTGGGCGACCAACGCT
ACCGATCCTCGGTAATTTGCTGATCAACGTTGAAAATGGTCAACTGTCAATGACCGCCACGGACTTAGAAGTGGAGCTGA
TCAGTAAAGTGACCTTGGAAGGGGAGTTTGAAGCGGGCAGTATTACGGTTCCTTCGCGCAAGTTTATCGATATCTGCCGT
GGATTACCCGACGATGCGGTGATCACGTTTGTATTGGAAGGTGATCGCGTGCAAGTGCGCTCCGGGCGAAGCCGTTTCTC
GTTGGCCACTCTGCCGGCCAATGATTTTCCGAATATTGAAGATTGGCAAAGTGAAGTGGAAGTCTCCCTCACTCAGGGCG
AATTGCGCGCGCTGATTGAGAAAACACAATTCTCGATGGCGAACCAAGACGTACGCTACTACCTCAATGGCATGTTGTTT
GAAATTGAGGGCAGCACACTGCGCAGTGTCGCGACCGATGGTCACCGTATGGCTGTGGCGCAGGCAGCGTTGAGTGCTGA
CTTTGCACAAAAGCAGATCATCGTACCGCGTAAAGGTGTGCAAGAGCTGGTTAAATTGCTGGATACACCAGAGCAACCCG
TCACGCTGCAAATTGGCAGCTCAAATCTGCGCGCCGAAGTAAACCATTTTGTCTTTACTTCTAAGTTGGTGGATGGTCGT
TTCCCGGATTACCGTCGCGTGTTGCCGCAACACACAACCAAAACGCTGGAAGCGAGTTGTGATGAATTACGCCAAGCGTT
TTCACGCGCGGCGATTTTGTCGAATGAAAAATTCCGTGGCGTTCGCGTTAACCTCGCTGGCAGTGAAATGCGCATTACCG
CCAACAACCCAGAGCAAGAAGAAGCGGAAGAGATGTTGGATGTGAGCTTCGAGGGCGAGGCGATTGAGATTGGCTTCAAC
GTTAGCTACGTATTGGATGTGTTGAACACCTTGCGTTGTGAACAAGTACGAATCTCAATGTCGGATGCCAACGCCAGTGC
TCTGGTTGAAAATGTCGATGACGACAGCGCCATGTACGTGGTTATGCCAATTCGTCTATAA

Upstream 100 bases:

>100_bases
AGAAGATTACTCTAACTTGATTCGCACCCTGTCGTCTTAATCGGTTAGAATGACACAATTCTAAACACTTTATTGAAGCG
GACAGCGTAAAGAGCCAAGC

Downstream 100 bases:

>100_bases
GCTGATTGAATGCCGCTTTCTCGTCTGATTATTCAGCAGTTTCGTAATATCAAAGCCTGCGACATTGCACTGTCGCCAGG
CTTTAACTTTCTTATTGGTC

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MKFTIARSHFIKPLQQVSGALGGRPTLPILGNLLINVENGQLSMTATDLEVELISKVTLEGEFEAGSITVPSRKFIDICR
GLPDDAVITFVLEGDRVQVRSGRSRFSLATLPANDFPNIEDWQSEVEVSLTQGELRALIEKTQFSMANQDVRYYLNGMLF
EIEGSTLRSVATDGHRMAVAQAALSADFAQKQIIVPRKGVQELVKLLDTPEQPVTLQIGSSNLRAEVNHFVFTSKLVDGR
FPDYRRVLPQHTTKTLEASCDELRQAFSRAAILSNEKFRGVRVNLAGSEMRITANNPEQEEAEEMLDVSFEGEAIEIGFN
VSYVLDVLNTLRCEQVRISMSDANASALVENVDDDSAMYVVMPIRL

Sequences:

>Translated_366_residues
MKFTIARSHFIKPLQQVSGALGGRPTLPILGNLLINVENGQLSMTATDLEVELISKVTLEGEFEAGSITVPSRKFIDICR
GLPDDAVITFVLEGDRVQVRSGRSRFSLATLPANDFPNIEDWQSEVEVSLTQGELRALIEKTQFSMANQDVRYYLNGMLF
EIEGSTLRSVATDGHRMAVAQAALSADFAQKQIIVPRKGVQELVKLLDTPEQPVTLQIGSSNLRAEVNHFVFTSKLVDGR
FPDYRRVLPQHTTKTLEASCDELRQAFSRAAILSNEKFRGVRVNLAGSEMRITANNPEQEEAEEMLDVSFEGEAIEIGFN
VSYVLDVLNTLRCEQVRISMSDANASALVENVDDDSAMYVVMPIRL
>Mature_366_residues
MKFTIARSHFIKPLQQVSGALGGRPTLPILGNLLINVENGQLSMTATDLEVELISKVTLEGEFEAGSITVPSRKFIDICR
GLPDDAVITFVLEGDRVQVRSGRSRFSLATLPANDFPNIEDWQSEVEVSLTQGELRALIEKTQFSMANQDVRYYLNGMLF
EIEGSTLRSVATDGHRMAVAQAALSADFAQKQIIVPRKGVQELVKLLDTPEQPVTLQIGSSNLRAEVNHFVFTSKLVDGR
FPDYRRVLPQHTTKTLEASCDELRQAFSRAAILSNEKFRGVRVNLAGSEMRITANNPEQEEAEEMLDVSFEGEAIEIGFN
VSYVLDVLNTLRCEQVRISMSDANASALVENVDDDSAMYVVMPIRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=366, Percent_Identity=63.9344262295082, Blast_Score=500, Evalue=1e-143,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40585; Mature: 40585

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTIARSHFIKPLQQVSGALGGRPTLPILGNLLINVENGQLSMTATDLEVELISKVTLE
CEEEECHHHHHHHHHHHHHHCCCCCCHHEECEEEEEEECCEEEEEEECCEEEEEHEEEEC
GEFEAGSITVPSRKFIDICRGLPDDAVITFVLEGDRVQVRSGRSRFSLATLPANDFPNIE
CCCCCCCEECCCHHHHHHHCCCCCCCEEEEEEECCEEEEECCCCEEEEEECCCCCCCCHH
DWQSEVEVSLTQGELRALIEKTQFSMANQDVRYYLNGMLFEIEGSTLRSVATDGHRMAVA
HHHHHEEEEECHHHHHHHHHHHHHHHCCCHHHEEECCEEEEECCCHHHHHHCCCHHHHHH
QAALSADFAQKQIIVPRKGVQELVKLLDTPEQPVTLQIGSSNLRAEVNHFVFTSKLVDGR
HHHHHHHHHHCEEECCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHEEEEHHHCCC
FPDYRRVLPQHTTKTLEASCDELRQAFSRAAILSNEKFRGVRVNLAGSEMRITANNPEQE
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCHH
EAEEMLDVSFEGEAIEIGFNVSYVLDVLNTLRCEQVRISMSDANASALVENVDDDSAMYV
HHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCEEEEEEEECCCCHHHHHCCCCCCCEEEE
VMPIRL
EEEEEC
>Mature Secondary Structure
MKFTIARSHFIKPLQQVSGALGGRPTLPILGNLLINVENGQLSMTATDLEVELISKVTLE
CEEEECHHHHHHHHHHHHHHCCCCCCHHEECEEEEEEECCEEEEEEECCEEEEEHEEEEC
GEFEAGSITVPSRKFIDICRGLPDDAVITFVLEGDRVQVRSGRSRFSLATLPANDFPNIE
CCCCCCCEECCCHHHHHHHCCCCCCCEEEEEEECCEEEEECCCCEEEEEECCCCCCCCHH
DWQSEVEVSLTQGELRALIEKTQFSMANQDVRYYLNGMLFEIEGSTLRSVATDGHRMAVA
HHHHHEEEEECHHHHHHHHHHHHHHHCCCHHHEEECCEEEEECCCHHHHHHCCCHHHHHH
QAALSADFAQKQIIVPRKGVQELVKLLDTPEQPVTLQIGSSNLRAEVNHFVFTSKLVDGR
HHHHHHHHHHCEEECCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHEEEEHHHCCC
FPDYRRVLPQHTTKTLEASCDELRQAFSRAAILSNEKFRGVRVNLAGSEMRITANNPEQE
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCHH
EAEEMLDVSFEGEAIEIGFNVSYVLDVLNTLRCEQVRISMSDANASALVENVDDDSAMYV
HHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCEEEEEEEECCCCHHHHHCCCCCCCEEEE
VMPIRL
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]