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The map label for this gene is recF

Identifier: 27364431

GI number: 27364431

Start: 1001575

End: 1002654

Strand: Reverse

Name: recF

Synonym: VV1_0997

Alternate gene names: 27364431

Gene position: 1002654-1001575 (Counterclockwise)

Preceding gene: 27364432

Following gene: 27364430

Centisome position: 30.55

GC content: 46.57

Gene sequence:

>1080_bases
ATGCCGCTTTCTCGTCTGATTATTCAGCAGTTTCGTAATATCAAAGCCTGCGACATTGCACTGTCGCCAGGCTTTAACTT
TCTTATTGGTCCGAATGGCAGTGGCAAAACCAGTGTCTTGGAAGCCATCTATTTGCTCGGTCATGGGCGCTCATTTAAAA
GTGCTTTGACCGGACGCGTGATTCAAAATGAGTGTGATCAACTGTTTGTCTATGGTCGTTTTTTGAACTCGGATCAATTT
GAGCTACCGATCGGCATTAATAAGCAGCGTGATGGCACAACAGAGGTTAAAATAGGCGGTCAATCTGGGCAAAAGTTAGC
GCAGCTAGCACAAGTGTTGCCATTGCAATTGATTCATCCCGAAGGCTTCGATTTATTAACGGATGGGCCCAAGCATCGTC
GAGCGTTTATTGATTGGGGCGTGTTTCATACTGAGCCCGCGTTTTATGATGCATGGGGGCGTTTTAAGCGCCTCAACAAA
CAACGTAATGCGTTGTTGAAGTCGGCAAAGAGCTATCAAGAGCTGAGTTATTGGGACAAGGAAATGGCACGTTTGGCCGA
GTTGATTAGCCAATGGCGCGCCGATTACGTTGCCCAAATGCAAAGCAAAGCGGAACAGTTGTGCCAAGAATTTTTGCCAG
AATTTCATATCCAATTGAAGTATTATCGTGGCTGGGAAAAAGAAACCCCATACCAACAAATACTGGAAGAGAATTTCGAG
CGCGATCAAACGCTAGGCTATACCGTTAGCGGGCCAAATAAGGCCGATTTACGGATAAAAGTGAATAACACCCCGGTGGA
AGATGTGTTATCGCGTGGCCAATTGAAATTAATGGTGTGTGCGCTCCGCTTGGCACAAGGCCAGCACCTAACGGAAAAAA
CCGGTAAGCAATGCGTCTATCTCATAGATGACTTTGCCTCTGAATTAGACAGTCAACGTCGCAAGCGTCTTGCTGATTGC
TTGAAACAGACGGGGGCTCAAGTTTTTGTAAGTTCGATTACGGAAAACCAGATCAGCGACATGCGCGATGACTCTGGCCG
ACTGTTTCATGTGGAGCAGGGTGTAATCGAGCAAGGATAA

Upstream 100 bases:

>100_bases
AATCTCAATGTCGGATGCCAACGCCAGTGCTCTGGTTGAAAATGTCGATGACGACAGCGCCATGTACGTGGTTATGCCAA
TTCGTCTATAAGCTGATTGA

Downstream 100 bases:

>100_bases
ATAATGAGAGAGTAACTCATGTCGAACAATTACGATTCATCGAGTATTAAGGTACTGAAGGGTCTGGATGCGGTACGTAA
GCGTCCGGGTATGTACATCG

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MPLSRLIIQQFRNIKACDIALSPGFNFLIGPNGSGKTSVLEAIYLLGHGRSFKSALTGRVIQNECDQLFVYGRFLNSDQF
ELPIGINKQRDGTTEVKIGGQSGQKLAQLAQVLPLQLIHPEGFDLLTDGPKHRRAFIDWGVFHTEPAFYDAWGRFKRLNK
QRNALLKSAKSYQELSYWDKEMARLAELISQWRADYVAQMQSKAEQLCQEFLPEFHIQLKYYRGWEKETPYQQILEENFE
RDQTLGYTVSGPNKADLRIKVNNTPVEDVLSRGQLKLMVCALRLAQGQHLTEKTGKQCVYLIDDFASELDSQRRKRLADC
LKQTGAQVFVSSITENQISDMRDDSGRLFHVEQGVIEQG

Sequences:

>Translated_359_residues
MPLSRLIIQQFRNIKACDIALSPGFNFLIGPNGSGKTSVLEAIYLLGHGRSFKSALTGRVIQNECDQLFVYGRFLNSDQF
ELPIGINKQRDGTTEVKIGGQSGQKLAQLAQVLPLQLIHPEGFDLLTDGPKHRRAFIDWGVFHTEPAFYDAWGRFKRLNK
QRNALLKSAKSYQELSYWDKEMARLAELISQWRADYVAQMQSKAEQLCQEFLPEFHIQLKYYRGWEKETPYQQILEENFE
RDQTLGYTVSGPNKADLRIKVNNTPVEDVLSRGQLKLMVCALRLAQGQHLTEKTGKQCVYLIDDFASELDSQRRKRLADC
LKQTGAQVFVSSITENQISDMRDDSGRLFHVEQGVIEQG
>Mature_358_residues
PLSRLIIQQFRNIKACDIALSPGFNFLIGPNGSGKTSVLEAIYLLGHGRSFKSALTGRVIQNECDQLFVYGRFLNSDQFE
LPIGINKQRDGTTEVKIGGQSGQKLAQLAQVLPLQLIHPEGFDLLTDGPKHRRAFIDWGVFHTEPAFYDAWGRFKRLNKQ
RNALLKSAKSYQELSYWDKEMARLAELISQWRADYVAQMQSKAEQLCQEFLPEFHIQLKYYRGWEKETPYQQILEENFER
DQTLGYTVSGPNKADLRIKVNNTPVEDVLSRGQLKLMVCALRLAQGQHLTEKTGKQCVYLIDDFASELDSQRRKRLADCL
KQTGAQVFVSSITENQISDMRDDSGRLFHVEQGVIEQG

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=356, Percent_Identity=56.4606741573034, Blast_Score=431, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_VIBVU (Q8DDJ1)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759959.1
- ProteinModelPortal:   Q8DDJ1
- SMR:   Q8DDJ1
- GeneID:   1177932
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0997
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   VVUL216895:VV1_0997-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 40997; Mature: 40866

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSRLIIQQFRNIKACDIALSPGFNFLIGPNGSGKTSVLEAIYLLGHGRSFKSALTGRV
CCHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
IQNECDQLFVYGRFLNSDQFELPIGINKQRDGTTEVKIGGQSGQKLAQLAQVLPLQLIHP
HHHHHHHEEHHHEECCCCCEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCEEECC
EGFDLLTDGPKHRRAFIDWGVFHTEPAFYDAWGRFKRLNKQRNALLKSAKSYQELSYWDK
CCCCCCCCCHHHHHHEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMARLAELISQWRADYVAQMQSKAEQLCQEFLPEFHIQLKYYRGWEKETPYQQILEENFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEEEECCCCCCCCHHHHHHHHHC
RDQTLGYTVSGPNKADLRIKVNNTPVEDVLSRGQLKLMVCALRLAQGQHLTEKTGKQCVY
CCCCCCEEECCCCCCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCCCEEH
LIDDFASELDSQRRKRLADCLKQTGAQVFVSSITENQISDMRDDSGRLFHVEQGVIEQG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
PLSRLIIQQFRNIKACDIALSPGFNFLIGPNGSGKTSVLEAIYLLGHGRSFKSALTGRV
CHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
IQNECDQLFVYGRFLNSDQFELPIGINKQRDGTTEVKIGGQSGQKLAQLAQVLPLQLIHP
HHHHHHHEEHHHEECCCCCEEECCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCCEEECC
EGFDLLTDGPKHRRAFIDWGVFHTEPAFYDAWGRFKRLNKQRNALLKSAKSYQELSYWDK
CCCCCCCCCHHHHHHEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMARLAELISQWRADYVAQMQSKAEQLCQEFLPEFHIQLKYYRGWEKETPYQQILEENFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEEEECCCCCCCCHHHHHHHHHC
RDQTLGYTVSGPNKADLRIKVNNTPVEDVLSRGQLKLMVCALRLAQGQHLTEKTGKQCVY
CCCCCCEEECCCCCCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCCCEEH
LIDDFASELDSQRRKRLADCLKQTGAQVFVSSITENQISDMRDDSGRLFHVEQGVIEQG
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA