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The map label for this gene is gspD [H]

Identifier: 27364325

GI number: 27364325

Start: 884417

End: 886438

Strand: Reverse

Name: gspD [H]

Synonym: VV1_0877

Alternate gene names: 27364325

Gene position: 886438-884417 (Counterclockwise)

Preceding gene: 326423781

Following gene: 27364324

Centisome position: 27.01

GC content: 48.66

Gene sequence:

>2022_bases
GTGAAACATTGGTTTAGCAAAAGCGCTTGGTTACTCGCAGGTACCTTAGCCTGCTCAAGCGGCGTGCTGGCAAACGAGTT
TAGTGCAAGCTTTAAAGGCACCGATATTCAAGAGTTCATCAATATTGTCGGTCGAAACTTAGAAAAGACCATCATTGTCG
ACCCTTCGGTGCGAGGCAAAATTGATGTACGCAGCTACGACGTATTAAATGAAGAGCAGTACTACAGCTTTTTCCTAAAC
GTACTTGAAGTGTATGGCTACGCGGTGGTTGAAATGGAAAATGGCGTACTGAAAGTCGTCAAATCCAAAGATTCCAAAAC
CTCCGCCATTCCCGTGGTCAGTGATGACACCGTGAAGGGCGACAATGTCATTACACGCGTGGTTGCGGTGCGCAATGTCT
CCGTGCGAGAGCTGTCTCCTTTGCTACGCCAACTGATTGATAATGCGGGCGCTGGTAACGTGGTGCACTATGATCCGGCC
AATATCATCTTGATCACGGGCCGTGCAGCGGTGGTGAATCGTTTGGCAGAAATCATCAAACGTGTGGACCAAGCGGGCGA
TACCGAAATCGAAGTGGTAGAGCTAGGTAATGCGTCGGCGGCGGAAATGGTCCGTATTGTTGATGCGCTAAACCGCACTA
CCGATGCGAAAAACACCCCAGAATTTTTGCAGCCAAAATTGGTGGCCGATGAGCGCACCAACTCGATTTTGATTTCGGGT
GATCCCAAAGTGCGTGATCGCTTGAAGCGTTTGATTCGCCAACTTGATGTGGAAATGGCGAGCAAAGGCAACAACCGTGT
TGTTTACCTCAAGTACGCCAAAGCGGAAGACTTAGTCGACGTATTGAAAGGGGTCTCAGACAACTTACAAGCAGAGAAAA
ACTCTGGTCAAAAGGGCGCGTCTTCACAGCGCAACGATGTGGTGATCGCCGCGCATCAAGGCACCAACTCACTGGTATTG
ACTGCGCCACCCGATATTATGTTGGCGCTGCAAGATGTGATTACCCAGTTAGATATTCGCCGTGCACAAGTGCTGATTGA
AGCCTTGATTGTCGAAATGGCAGAGGGCGATGGTGTGAACTTGGGTGTGCAATGGGGTAACCTGGAAACGGGTGCGATGA
TCCAGTACAGTAATACAGGGGCATCGATTGGCCAAGTAATGATTGGTCTAGAGGAAGCAAAGGATACAACTAAGACTGAA
AGCCGTTGGGATCCAGATAAAAATCAGTGGGTGGATCGTCAGTACACTGAAAAAGGGGACTATTCAACACTGGCTTCTGC
ATTGTCTAAGGTCAATGGCGCGGCCCTCAGTGTCGTGATGGGTGACTGGACGGCGTTGATTAGCGCGGTATCGAGTGATT
CCAACTCAAATATCCTGTCTTCCCCAAGTATTACCGTAATGGATAACGGTGAAGCCTCATTTATTGTGGGTGAAGAAGTA
CCGGTCATCACAGGCTCCACAGCGGGGTCCAATAACGACAACCCATTCCAAACCGTTGACCGCAAAGAAGTGGGCATCAA
GCTTAAAGTGGTGCCACAAATTAACGAAGGCGACTCGGTTCAACTGAATATTGAACAGGAAGTGTCAAACGTTTTGGGTG
CCAATGGCGCGGTGGACGTACGTTTTGCTAAGCGTCAGCTCAATACCTCGGTGATTGTGCAAGATGGCCAAATGTTGGTG
CTCGGCGGCTTGATTGATGAACGTGCGCTAGAGAGTGAATCAAAAGTGCCACTGCTAGGGGATATTCCCGTGCTGGGGCA
TTTGTTTAAATCAACCAATACTCAAGTTGAAAAGAAAAACCTGATGGTGTTCATCAAGCCAACTATCATTCGTGATGGCA
TGACGGCCGATGGTATTACCCAGCGTAAGTACAACTACATTCGCGCCGAACAGCTTTACAAAGCGGATCAGGGCCTTAAA
TTGATGGATGATGGCCACATTCCTGTGTTGCCGAAATTTGGTGAAGACAAACGCCATCCGGCTGAAATTCAAGCCTTTAT
CGATCAAATGGAACAGCAGTAA

Upstream 100 bases:

>100_bases
CACCGAGTTGAACCTCACGGTGGAACGAGACGGACAGCAGCATGATATTTACATCCAGTTTTAACACTTAGGCTCAAAGC
GTAAGTTCAGGGAGATAAAC

Downstream 100 bases:

>100_bases
TGGTAGATATACTGGATACGGCGCCATCGTATCGCCGTTTACCGTTTAGTTTTGCTAACCGCTTTAAAATGGTGCTGGAA
GTCGAGCACCCAGAAAGGCC

Product: general secretion pathway protein D

Products: NA

Alternate protein names: Cholera toxin secretion protein epsD [H]

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MKHWFSKSAWLLAGTLACSSGVLANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGKIDVRSYDVLNEEQYYSFFLN
VLEVYGYAVVEMENGVLKVVKSKDSKTSAIPVVSDDTVKGDNVITRVVAVRNVSVRELSPLLRQLIDNAGAGNVVHYDPA
NIILITGRAAVVNRLAEIIKRVDQAGDTEIEVVELGNASAAEMVRIVDALNRTTDAKNTPEFLQPKLVADERTNSILISG
DPKVRDRLKRLIRQLDVEMASKGNNRVVYLKYAKAEDLVDVLKGVSDNLQAEKNSGQKGASSQRNDVVIAAHQGTNSLVL
TAPPDIMLALQDVITQLDIRRAQVLIEALIVEMAEGDGVNLGVQWGNLETGAMIQYSNTGASIGQVMIGLEEAKDTTKTE
SRWDPDKNQWVDRQYTEKGDYSTLASALSKVNGAALSVVMGDWTALISAVSSDSNSNILSSPSITVMDNGEASFIVGEEV
PVITGSTAGSNNDNPFQTVDRKEVGIKLKVVPQINEGDSVQLNIEQEVSNVLGANGAVDVRFAKRQLNTSVIVQDGQMLV
LGGLIDERALESESKVPLLGDIPVLGHLFKSTNTQVEKKNLMVFIKPTIIRDGMTADGITQRKYNYIRAEQLYKADQGLK
LMDDGHIPVLPKFGEDKRHPAEIQAFIDQMEQQ

Sequences:

>Translated_673_residues
MKHWFSKSAWLLAGTLACSSGVLANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGKIDVRSYDVLNEEQYYSFFLN
VLEVYGYAVVEMENGVLKVVKSKDSKTSAIPVVSDDTVKGDNVITRVVAVRNVSVRELSPLLRQLIDNAGAGNVVHYDPA
NIILITGRAAVVNRLAEIIKRVDQAGDTEIEVVELGNASAAEMVRIVDALNRTTDAKNTPEFLQPKLVADERTNSILISG
DPKVRDRLKRLIRQLDVEMASKGNNRVVYLKYAKAEDLVDVLKGVSDNLQAEKNSGQKGASSQRNDVVIAAHQGTNSLVL
TAPPDIMLALQDVITQLDIRRAQVLIEALIVEMAEGDGVNLGVQWGNLETGAMIQYSNTGASIGQVMIGLEEAKDTTKTE
SRWDPDKNQWVDRQYTEKGDYSTLASALSKVNGAALSVVMGDWTALISAVSSDSNSNILSSPSITVMDNGEASFIVGEEV
PVITGSTAGSNNDNPFQTVDRKEVGIKLKVVPQINEGDSVQLNIEQEVSNVLGANGAVDVRFAKRQLNTSVIVQDGQMLV
LGGLIDERALESESKVPLLGDIPVLGHLFKSTNTQVEKKNLMVFIKPTIIRDGMTADGITQRKYNYIRAEQLYKADQGLK
LMDDGHIPVLPKFGEDKRHPAEIQAFIDQMEQQ
>Mature_673_residues
MKHWFSKSAWLLAGTLACSSGVLANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGKIDVRSYDVLNEEQYYSFFLN
VLEVYGYAVVEMENGVLKVVKSKDSKTSAIPVVSDDTVKGDNVITRVVAVRNVSVRELSPLLRQLIDNAGAGNVVHYDPA
NIILITGRAAVVNRLAEIIKRVDQAGDTEIEVVELGNASAAEMVRIVDALNRTTDAKNTPEFLQPKLVADERTNSILISG
DPKVRDRLKRLIRQLDVEMASKGNNRVVYLKYAKAEDLVDVLKGVSDNLQAEKNSGQKGASSQRNDVVIAAHQGTNSLVL
TAPPDIMLALQDVITQLDIRRAQVLIEALIVEMAEGDGVNLGVQWGNLETGAMIQYSNTGASIGQVMIGLEEAKDTTKTE
SRWDPDKNQWVDRQYTEKGDYSTLASALSKVNGAALSVVMGDWTALISAVSSDSNSNILSSPSITVMDNGEASFIVGEEV
PVITGSTAGSNNDNPFQTVDRKEVGIKLKVVPQINEGDSVQLNIEQEVSNVLGANGAVDVRFAKRQLNTSVIVQDGQMLV
LGGLIDERALESESKVPLLGDIPVLGHLFKSTNTQVEKKNLMVFIKPTIIRDGMTADGITQRKYNYIRAEQLYKADQGLK
LMDDGHIPVLPKFGEDKRHPAEIQAFIDQMEQQ

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=672, Percent_Identity=44.3452380952381, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1789793, Length=351, Percent_Identity=25.6410256410256, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845
- InterPro:   IPR001814 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 73492; Mature: 73492

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHWFSKSAWLLAGTLACSSGVLANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGK
CCCCCCCCCCEEEEHHHHCCCCHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCE
IDVRSYDVLNEEQYYSFFLNVLEVYGYAVVEMENGVLKVVKSKDSKTSAIPVVSDDTVKG
EEEEEECCCCHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCCCEEEEECCCCCCC
DNVITRVVAVRNVSVRELSPLLRQLIDNAGAGNVVHYDPANIILITGRAAVVNRLAEIIK
CHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHH
RVDQAGDTEIEVVELGNASAAEMVRIVDALNRTTDAKNTPEFLQPKLVADERTNSILISG
HHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCCCEEEEEC
DPKVRDRLKRLIRQLDVEMASKGNNRVVYLKYAKAEDLVDVLKGVSDNLQAEKNSGQKGA
CHHHHHHHHHHHHHHCHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCC
SSQRNDVVIAAHQGTNSLVLTAPPDIMLALQDVITQLDIRRAQVLIEALIVEMAEGDGVN
CCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LGVQWGNLETGAMIQYSNTGASIGQVMIGLEEAKDTTKTESRWDPDKNQWVDRQYTEKGD
EEEEECCCCCCEEEEECCCCCCHHHHEEEHHHHCCCCCCCCCCCCCHHCCCCCHHCCCCC
YSTLASALSKVNGAALSVVMGDWTALISAVSSDSNSNILSSPSITVMDNGEASFIVGEEV
HHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCEECCCCEEEEECCCEEEEECCCC
PVITGSTAGSNNDNPFQTVDRKEVGIKLKVVPQINEGDSVQLNIEQEVSNVLGANGAVDV
CEEECCCCCCCCCCCCCCCCHHHCCEEEEEEEECCCCCEEEEEEHHHHHHHHCCCCCEEE
RFAKRQLNTSVIVQDGQMLVLGGLIDERALESESKVPLLGDIPVLGHLFKSTNTQVEKKN
EEHHHHCCCEEEEECCCEEEEECHHHHHHHCCCCCCCEEECCHHHHHHHHCCCCEEECCC
LMVFIKPTIIRDGMTADGITQRKYNYIRAEQLYKADQGLKLMDDGHIPVLPKFGEDKRHP
EEEEEECEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCH
AEIQAFIDQMEQQ
HHHHHHHHHHHCC
>Mature Secondary Structure
MKHWFSKSAWLLAGTLACSSGVLANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGK
CCCCCCCCCCEEEEHHHHCCCCHHHHCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCE
IDVRSYDVLNEEQYYSFFLNVLEVYGYAVVEMENGVLKVVKSKDSKTSAIPVVSDDTVKG
EEEEEECCCCHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCCCEEEEECCCCCCC
DNVITRVVAVRNVSVRELSPLLRQLIDNAGAGNVVHYDPANIILITGRAAVVNRLAEIIK
CHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHH
RVDQAGDTEIEVVELGNASAAEMVRIVDALNRTTDAKNTPEFLQPKLVADERTNSILISG
HHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCCCEEEEEC
DPKVRDRLKRLIRQLDVEMASKGNNRVVYLKYAKAEDLVDVLKGVSDNLQAEKNSGQKGA
CHHHHHHHHHHHHHHCHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCC
SSQRNDVVIAAHQGTNSLVLTAPPDIMLALQDVITQLDIRRAQVLIEALIVEMAEGDGVN
CCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LGVQWGNLETGAMIQYSNTGASIGQVMIGLEEAKDTTKTESRWDPDKNQWVDRQYTEKGD
EEEEECCCCCCEEEEECCCCCCHHHHEEEHHHHCCCCCCCCCCCCCHHCCCCCHHCCCCC
YSTLASALSKVNGAALSVVMGDWTALISAVSSDSNSNILSSPSITVMDNGEASFIVGEEV
HHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCEECCCCEEEEECCCEEEEECCCC
PVITGSTAGSNNDNPFQTVDRKEVGIKLKVVPQINEGDSVQLNIEQEVSNVLGANGAVDV
CEEECCCCCCCCCCCCCCCCHHHCCEEEEEEEECCCCCEEEEEEHHHHHHHHCCCCCEEE
RFAKRQLNTSVIVQDGQMLVLGGLIDERALESESKVPLLGDIPVLGHLFKSTNTQVEKKN
EEHHHHCCCEEEEECCCEEEEECHHHHHHHCCCCCCCEEECCHHHHHHHHCCCCEEECCC
LMVFIKPTIIRDGMTADGITQRKYNYIRAEQLYKADQGLKLMDDGHIPVLPKFGEDKRHP
EEEEEECEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCH
AEIQAFIDQMEQQ
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]