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The map label for this gene is gspE [H]

Identifier: 27364324

GI number: 27364324

Start: 882918

End: 884417

Strand: Reverse

Name: gspE [H]

Synonym: VV1_0876

Alternate gene names: 27364324

Gene position: 884417-882918 (Counterclockwise)

Preceding gene: 27364325

Following gene: 27364323

Centisome position: 26.95

GC content: 51.4

Gene sequence:

>1500_bases
ATGGTAGATATACTGGATACGGCGCCATCGTATCGCCGTTTACCGTTTAGTTTTGCTAACCGCTTTAAAATGGTGCTGGA
AGTCGAGCACCCAGAAAGGCCGCCGGTACTTTACTACGTTGAGCCTCTCAATGCGCAGGCGTTAGTTGAAGTGCGTCGTG
TGCTCAAACAGACGTTTGTCCCTCAAGCCATTGCCGCTGAAGCGTTTGAAAAAAAACTGACCGAAGCGTACCAACGCGAC
TCGTCAGAAGCGCGTCAGCTGATGGAGGACATTGGCTCGGATGATGACTTCTTCTCTTTGGCTGAAGAGTTGCCGCAAGA
TGAAGATTTGCTCGAAACGGAAGACGATGCGCCGATCATCAAATTGATCAACGCCATGTTGGGCGAAGCGATCAAAGAAG
GGGCGTCGGATATTCACATCGAAACGTTCGAGAAAATTCTGTCGATTCGTTTCCGCGTCGATGGCGTTTTGCGCGATGTA
CTGTCTCCGAGTCGCAAACTGGCGCCGCTGTTGGTGTCACGTGTTAAAGTTATGGCCAAACTGGATATTGCAGAAAAACG
TGTGCCACAAGATGGCCGTATTTCACTGCGTATCGGTGGTCGCGCTGTCGATGTGCGTGTATCGACCATGCCTTCATCAC
ACGGCGAGCGTGTGGTGATGCGTTTGCTGGATAAAAACGCCACTCGTCTTGACCTGCATAGCCTAGGCATGACACCGGTG
AACCACGACAATTTCCGTCATCTTATCTCGCGTCCTCACGGCATTATCTTGGTCACAGGTCCAACGGGTTCGGGTAAATC
CACCACCTTGTACGCTGGCCTGCAAGAGCTGAACAGCAACGAGCGCAACATCTTAACGGTGGAAGACCCGATCGAATTTG
ATATTGATGGTATCGGCCAAACCCAAGTGAACCCAAAAGTCGACATGACCTTTGCTCGTGGTCTGCGAGCCATTTTGCGT
CAAGACCCAGACGTGGTGATGGTCGGGGAAATTCGTGACTTGGAAACCGCGCAAATTGCCGTACAAGCGTCATTGACCGG
TCACTTGGTGATGTCAACCTTGCACACCAACACCGCCGTAGGTGCAATTACCCGTCTGCGTGACATGGGCATTGAGCCCT
TTTTGATCTCTTCTTCACTGCTGGGCGTGCTGGCTCAGCGCCTGGTACGGACTTTATGCCAAGACTGTAAAGAACCGTAC
GAGGCGGACAAAGAGCAGCGCAAACTGTTTGGCGCGAAGAAGAAAGATGCGCTGACCTTATATCGTGCCAAGGGATGTGA
AAAATGTAACCACAAAGGTTATCGTGGCCGTACCGGTATCCATGAGCTGTTGATGGTGGACGATAAAGTTCAAGAGCTGA
TCCATGCTGAAGCGGGAGAGCAGGCGATGGACAAATACATTCGTGAACATACCCCGAGCATTCGCAGCGACGGTTTAGAC
AAAGTGTTGCAAGGCGTGACGTCACTGGAAGAAGTGATGCGCGTGACCAAGGAATCTTAA

Upstream 100 bases:

>100_bases
TGATGGATGATGGCCACATTCCTGTGTTGCCGAAATTTGGTGAAGACAAACGCCATCCGGCTGAAATTCAAGCCTTTATC
GATCAAATGGAACAGCAGTA

Downstream 100 bases:

>100_bases
TGGCGGCGTTTGAATACAAAGCGCTCGACGCAAAAGGGCGCACCAAAAAAGGCACCTTGGAAGGCGACAATGCCCGCCAA
GTACGCCAACGCTTGAAAGA

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MVDILDTAPSYRRLPFSFANRFKMVLEVEHPERPPVLYYVEPLNAQALVEVRRVLKQTFVPQAIAAEAFEKKLTEAYQRD
SSEARQLMEDIGSDDDFFSLAEELPQDEDLLETEDDAPIIKLINAMLGEAIKEGASDIHIETFEKILSIRFRVDGVLRDV
LSPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTPV
NHDNFRHLISRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILR
QDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCQDCKEPY
EADKEQRKLFGAKKKDALTLYRAKGCEKCNHKGYRGRTGIHELLMVDDKVQELIHAEAGEQAMDKYIREHTPSIRSDGLD
KVLQGVTSLEEVMRVTKES

Sequences:

>Translated_499_residues
MVDILDTAPSYRRLPFSFANRFKMVLEVEHPERPPVLYYVEPLNAQALVEVRRVLKQTFVPQAIAAEAFEKKLTEAYQRD
SSEARQLMEDIGSDDDFFSLAEELPQDEDLLETEDDAPIIKLINAMLGEAIKEGASDIHIETFEKILSIRFRVDGVLRDV
LSPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTPV
NHDNFRHLISRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILR
QDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCQDCKEPY
EADKEQRKLFGAKKKDALTLYRAKGCEKCNHKGYRGRTGIHELLMVDDKVQELIHAEAGEQAMDKYIREHTPSIRSDGLD
KVLQGVTSLEEVMRVTKES
>Mature_499_residues
MVDILDTAPSYRRLPFSFANRFKMVLEVEHPERPPVLYYVEPLNAQALVEVRRVLKQTFVPQAIAAEAFEKKLTEAYQRD
SSEARQLMEDIGSDDDFFSLAEELPQDEDLLETEDDAPIIKLINAMLGEAIKEGASDIHIETFEKILSIRFRVDGVLRDV
LSPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTPV
NHDNFRHLISRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILR
QDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCQDCKEPY
EADKEQRKLFGAKKKDALTLYRAKGCEKCNHKGYRGRTGIHELLMVDDKVQELIHAEAGEQAMDKYIREHTPSIRSDGLD
KVLQGVTSLEEVMRVTKES

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=452, Percent_Identity=57.5221238938053, Blast_Score=544, Evalue=1e-156,
Organism=Escherichia coli, GI1786296, Length=385, Percent_Identity=43.3766233766234, Blast_Score=322, Evalue=3e-89,
Organism=Escherichia coli, GI87082188, Length=169, Percent_Identity=44.3786982248521, Blast_Score=119, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55847; Mature: 55847

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDILDTAPSYRRLPFSFANRFKMVLEVEHPERPPVLYYVEPLNAQALVEVRRVLKQTFV
CCCCCCCCCCCCCCCHHHHHHEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCC
PQAIAAEAFEKKLTEAYQRDSSEARQLMEDIGSDDDFFSLAEELPQDEDLLETEDDAPII
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCHHH
KLINAMLGEAIKEGASDIHIETFEKILSIRFRVDGVLRDVLSPSRKLAPLLVSRVKVMAK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTPV
HHHHHHCCCCCCEEEEEECCEEEEEEEEECCCCCHHHHHHHHHHCCCCEEEHHHCCCCCC
NHDNFRHLISRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQ
CCHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEECCCCCCC
TQVNPKVDMTFARGLRAILRQDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAV
CCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEHHHHHHHHHHHHHHHHH
GAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCQDCKEPYEADKEQRKLFGAKKKDALTL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHCCCCCHHHHH
YRAKGCEKCNHKGYRGRTGIHELLMVDDKVQELIHAEAGEQAMDKYIREHTPSIRSDGLD
HHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHCCCHH
KVLQGVTSLEEVMRVTKES
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVDILDTAPSYRRLPFSFANRFKMVLEVEHPERPPVLYYVEPLNAQALVEVRRVLKQTFV
CCCCCCCCCCCCCCCHHHHHHEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCC
PQAIAAEAFEKKLTEAYQRDSSEARQLMEDIGSDDDFFSLAEELPQDEDLLETEDDAPII
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCHHH
KLINAMLGEAIKEGASDIHIETFEKILSIRFRVDGVLRDVLSPSRKLAPLLVSRVKVMAK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTPV
HHHHHHCCCCCCEEEEEECCEEEEEEEEECCCCCHHHHHHHHHHCCCCEEEHHHCCCCCC
NHDNFRHLISRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQ
CCHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEECCCCCCC
TQVNPKVDMTFARGLRAILRQDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAV
CCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEHHHHHHHHHHHHHHHHH
GAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCQDCKEPYEADKEQRKLFGAKKKDALTL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHCCCCCHHHHH
YRAKGCEKCNHKGYRGRTGIHELLMVDDKVQELIHAEAGEQAMDKYIREHTPSIRSDGLD
HHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHCCCHH
KVLQGVTSLEEVMRVTKES
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]