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The map label for this gene is gspF [H]

Identifier: 27364323

GI number: 27364323

Start: 881701

End: 882918

Strand: Reverse

Name: gspF [H]

Synonym: VV1_0875

Alternate gene names: 27364323

Gene position: 882918-881701 (Counterclockwise)

Preceding gene: 27364324

Following gene: 27364322

Centisome position: 26.9

GC content: 50.99

Gene sequence:

>1218_bases
ATGGCGGCGTTTGAATACAAAGCGCTCGACGCAAAAGGGCGCACCAAAAAAGGCACCTTGGAAGGCGACAATGCCCGCCA
AGTACGCCAACGCTTGAAAGAGCAAGGCATGGTGCCCATCGAGGTGATGGAAACCAAAGCCAAGTTGGCCAAAAGCAAAT
CGTCAGGTGGCTTTAAGCGGGGCATTTCCACTCCCGAACTGTCGTTGATTACTCGTCAGATCTCGACCCTGGTGCAATCG
GGTATGCCGCTGGAGGAGTGTCTCAAAGCGGTATCCGATCAAGCAGAGAAGCCTCGTATTCGAGGTATGTTGGCAGCGGT
GCGGGCCAAAGTCACCGAAGGTTACACCTTGGCAGACAGCCTGTCAGACTACCCGCATATTTTTGATGAACTGTATCGCT
CTATGGTGGCGGCAGGGGAAAAATCGGGTCATTTGGATGCGGTGTTAGAGCGCTTGGCCGATTACTGTGAAAATCGCCAG
AAGATGCGTTCTAAGTTACTGCAAGCGATGATTTACCCGGTGGTATTGGTGGTGTTTGCTGTCACGATTGTGGCGTTTTT
GCTCGCCACAGTGGTACCCAAAATCGTCGAGCCGATCATCCAAATGGGTCAAGAGCTGCCACAGTCGACGCAATTTTTAT
TGGCGGCGAGTGAGTTTGTTCAAGAGTGGGGATTATTGCTGTTGGGAAGCATCGTGTTTGCCATTTACCTACTCAAAACG
GCCTTGAAAAAGCCCAATGTTCGCATGGCGTGGGATCGCCGCATACTGAGCCTGCCGCTACTGGGCAAAATCTCGAAAGG
GTTGAACACCGCTCGATTTGCTCGCACCTTATCCATTTGTACCTCCAGTGCGATTCCCATTTTGGAAGGGATGCGTGTGG
CGGTCGATGTGATGTCGAACCAATTCGTCAAACAACAGGTCTTATTGGCGGCAGACAGTGTGCGTGAAGGGGCCAGTCTG
CGTAAAGCTTTGGATCAAACTCGCCTTTTTCCACCAATGATGTTGCACATGATTGCCAGTGGAGAACAAAGTGGTGAGTT
GGAAAGCATGCTGACGCGTGCCGCGGACAACCAAGATCAAAGTTTTGAATCGACGGTCAATATCGCGTTGGGTATTTTTA
CCCCAGCGTTGATTGCGCTAATGGCGGGATTGGTGTTGTTCATTGTGATGGCGACACTGATGCCGATGCTGGAAATGAAT
AATTTAATGAGTGGCTGA

Upstream 100 bases:

>100_bases
TTCGTGAACATACCCCGAGCATTCGCAGCGACGGTTTAGACAAAGTGTTGCAAGGCGTGACGTCACTGGAAGAAGTGATG
CGCGTGACCAAGGAATCTTA

Downstream 100 bases:

>100_bases
TTTTGGGGTGCTGAGTGGCTTGGTCATTCGGCCTCATTATTCTGGAGAAAACAATGCAAAATAAAGCAAAAAAACAGGCG
GGTTTCACCCTGCTTGAAGT

Product: general secretion pathway protein F

Products: NA

Alternate protein names: Cholera toxin secretion protein epsF [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MAAFEYKALDAKGRTKKGTLEGDNARQVRQRLKEQGMVPIEVMETKAKLAKSKSSGGFKRGISTPELSLITRQISTLVQS
GMPLEECLKAVSDQAEKPRIRGMLAAVRAKVTEGYTLADSLSDYPHIFDELYRSMVAAGEKSGHLDAVLERLADYCENRQ
KMRSKLLQAMIYPVVLVVFAVTIVAFLLATVVPKIVEPIIQMGQELPQSTQFLLAASEFVQEWGLLLLGSIVFAIYLLKT
ALKKPNVRMAWDRRILSLPLLGKISKGLNTARFARTLSICTSSAIPILEGMRVAVDVMSNQFVKQQVLLAADSVREGASL
RKALDQTRLFPPMMLHMIASGEQSGELESMLTRAADNQDQSFESTVNIALGIFTPALIALMAGLVLFIVMATLMPMLEMN
NLMSG

Sequences:

>Translated_405_residues
MAAFEYKALDAKGRTKKGTLEGDNARQVRQRLKEQGMVPIEVMETKAKLAKSKSSGGFKRGISTPELSLITRQISTLVQS
GMPLEECLKAVSDQAEKPRIRGMLAAVRAKVTEGYTLADSLSDYPHIFDELYRSMVAAGEKSGHLDAVLERLADYCENRQ
KMRSKLLQAMIYPVVLVVFAVTIVAFLLATVVPKIVEPIIQMGQELPQSTQFLLAASEFVQEWGLLLLGSIVFAIYLLKT
ALKKPNVRMAWDRRILSLPLLGKISKGLNTARFARTLSICTSSAIPILEGMRVAVDVMSNQFVKQQVLLAADSVREGASL
RKALDQTRLFPPMMLHMIASGEQSGELESMLTRAADNQDQSFESTVNIALGIFTPALIALMAGLVLFIVMATLMPMLEMN
NLMSG
>Mature_404_residues
AAFEYKALDAKGRTKKGTLEGDNARQVRQRLKEQGMVPIEVMETKAKLAKSKSSGGFKRGISTPELSLITRQISTLVQSG
MPLEECLKAVSDQAEKPRIRGMLAAVRAKVTEGYTLADSLSDYPHIFDELYRSMVAAGEKSGHLDAVLERLADYCENRQK
MRSKLLQAMIYPVVLVVFAVTIVAFLLATVVPKIVEPIIQMGQELPQSTQFLLAASEFVQEWGLLLLGSIVFAIYLLKTA
LKKPNVRMAWDRRILSLPLLGKISKGLNTARFARTLSICTSSAIPILEGMRVAVDVMSNQFVKQQVLLAADSVREGASLR
KALDQTRLFPPMMLHMIASGEQSGELESMLTRAADNQDQSFESTVNIALGIFTPALIALMAGLVLFIVMATLMPMLEMNN
LMSG

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=401, Percent_Identity=41.3965087281795, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1786295, Length=405, Percent_Identity=22.2222222222222, Blast_Score=128, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 44528; Mature: 44397

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAFEYKALDAKGRTKKGTLEGDNARQVRQRLKEQGMVPIEVMETKAKLAKSKSSGGFKR
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHC
GISTPELSLITRQISTLVQSGMPLEECLKAVSDQAEKPRIRGMLAAVRAKVTEGYTLADS
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHH
LSDYPHIFDELYRSMVAAGEKSGHLDAVLERLADYCENRQKMRSKLLQAMIYPVVLVVFA
HCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTIVAFLLATVVPKIVEPIIQMGQELPQSTQFLLAASEFVQEWGLLLLGSIVFAIYLLKT
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKKPNVRMAWDRRILSLPLLGKISKGLNTARFARTLSICTSSAIPILEGMRVAVDVMSN
HHCCCCCEEHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QFVKQQVLLAADSVREGASLRKALDQTRLFPPMMLHMIASGEQSGELESMLTRAADNQDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCH
SFESTVNIALGIFTPALIALMAGLVLFIVMATLMPMLEMNNLMSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAFEYKALDAKGRTKKGTLEGDNARQVRQRLKEQGMVPIEVMETKAKLAKSKSSGGFKR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHC
GISTPELSLITRQISTLVQSGMPLEECLKAVSDQAEKPRIRGMLAAVRAKVTEGYTLADS
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHH
LSDYPHIFDELYRSMVAAGEKSGHLDAVLERLADYCENRQKMRSKLLQAMIYPVVLVVFA
HCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTIVAFLLATVVPKIVEPIIQMGQELPQSTQFLLAASEFVQEWGLLLLGSIVFAIYLLKT
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKKPNVRMAWDRRILSLPLLGKISKGLNTARFARTLSICTSSAIPILEGMRVAVDVMSN
HHCCCCCEEHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QFVKQQVLLAADSVREGASLRKALDQTRLFPPMMLHMIASGEQSGELESMLTRAADNQDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCH
SFESTVNIALGIFTPALIALMAGLVLFIVMATLMPMLEMNNLMSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]