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The map label for this gene is gspJ [H]

Identifier: 27364319

GI number: 27364319

Start: 879530

End: 880231

Strand: Reverse

Name: gspJ [H]

Synonym: VV1_0871

Alternate gene names: 27364319

Gene position: 880231-879530 (Counterclockwise)

Preceding gene: 27364320

Following gene: 27364318

Centisome position: 26.82

GC content: 51.14

Gene sequence:

>702_bases
ATGTGGCGAAATAAAACGTGTCGAAAGAGAAACACGCGCCAACGTGGTTTTACCCTCATCGAAGTGTTGGTGTCGATTGC
CATTTTTGCCACCTTAAGTGTGGCCGCGTATCAGGTGGTCAATCAGGTGCAACGCAGCAATGAGTTATCACAAGAGCGCA
CCGTACGATTGAATGAATTGCAACGTGCCTTGGTGATGATGGACAGCGATTTTCGCCAGATAGCGCTGCGTCAAACACGC
ACCAACGGCGAAGAGCCGAGCAAAAAACTGCTGCATTGGGCGGATTACCTCTTAGATTCAGACAGCAAAGGCATCATGTT
CGCGCGTTTAGGTTGGCACAATCCGCAGCAGCAATTCCCTCGCGGGGAGGTCACCAAAGTGGGTTATCGCATCAAAGATG
AGCGTTTAGAGCGAGTATGGTGGCGTTACCCAGATACCCCAGCAGGGCAAGAAGGGGTCGTGACTCCGTTACTCAGTGAT
GTGGAAGAGCTTAATGTGCGTTTTTATGACGGCAAACAGTGGAGTAATGAGTGGAGCAACGAGTTGACCTTGCCGGCGGC
GATCTCGGTAGAGCTGACGTTGAAAGATTACGGAAAGATCGCGCGAACCTACCTCACTCCAGAGGGGAATTTGCAAAAAC
AAGCGTCAGGTAGCGAAGACTCCGGGCAAAACAATAGTGGTGGAGCAGGCAACAATGGCTAA

Upstream 100 bases:

>100_bases
AAAGTGGCTCCTGTAGCCACGACTCAACCGCTGCTGAAAGCGTTTGATGTGAGTGTGGCAGCAGAAAAGCAGGCCAGCCC
AATGATTACGGTGAGAAGCT

Downstream 100 bases:

>100_bases
ATCGAACCGCTCGCGCCAATCTGGAGTGGCCCTGATCATCATTTTGCTGTTGCTGGCCATTATGGCCACCATCGCCGGCA
GTATGTCTGAGCGTTTGTTT

Product: general secretion pathway protein J

Products: NA

Alternate protein names: Cholera toxin secretion protein epsJ [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MWRNKTCRKRNTRQRGFTLIEVLVSIAIFATLSVAAYQVVNQVQRSNELSQERTVRLNELQRALVMMDSDFRQIALRQTR
TNGEEPSKKLLHWADYLLDSDSKGIMFARLGWHNPQQQFPRGEVTKVGYRIKDERLERVWWRYPDTPAGQEGVVTPLLSD
VEELNVRFYDGKQWSNEWSNELTLPAAISVELTLKDYGKIARTYLTPEGNLQKQASGSEDSGQNNSGGAGNNG

Sequences:

>Translated_233_residues
MWRNKTCRKRNTRQRGFTLIEVLVSIAIFATLSVAAYQVVNQVQRSNELSQERTVRLNELQRALVMMDSDFRQIALRQTR
TNGEEPSKKLLHWADYLLDSDSKGIMFARLGWHNPQQQFPRGEVTKVGYRIKDERLERVWWRYPDTPAGQEGVVTPLLSD
VEELNVRFYDGKQWSNEWSNELTLPAAISVELTLKDYGKIARTYLTPEGNLQKQASGSEDSGQNNSGGAGNNG
>Mature_233_residues
MWRNKTCRKRNTRQRGFTLIEVLVSIAIFATLSVAAYQVVNQVQRSNELSQERTVRLNELQRALVMMDSDFRQIALRQTR
TNGEEPSKKLLHWADYLLDSDSKGIMFARLGWHNPQQQFPRGEVTKVGYRIKDERLERVWWRYPDTPAGQEGVVTPLLSD
VEELNVRFYDGKQWSNEWSNELTLPAAISVELTLKDYGKIARTYLTPEGNLQKQASGSEDSGQNNSGGAGNNG

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG4795

COG function: function code U; Type II secretory pathway, component PulJ

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP J family [H]

Homologues:

Organism=Escherichia coli, GI1789728, Length=214, Percent_Identity=24.2990654205607, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010055
- InterPro:   IPR012902
- InterPro:   IPR001120 [H]

Pfam domain/function: PF07963 N_methyl [H]

EC number: NA

Molecular weight: Translated: 26650; Mature: 26650

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS00409 PROKAR_NTER_METHYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWRNKTCRKRNTRQRGFTLIEVLVSIAIFATLSVAAYQVVNQVQRSNELSQERTVRLNEL
CCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRALVMMDSDFRQIALRQTRTNGEEPSKKLLHWADYLLDSDSKGIMFARLGWHNPQQQFP
HHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCC
RGEVTKVGYRIKDERLERVWWRYPDTPAGQEGVVTPLLSDVEELNVRFYDGKQWSNEWSN
CCCHHHHCCEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCHHHHHCC
ELTLPAAISVELTLKDYGKIARTYLTPEGNLQKQASGSEDSGQNNSGGAGNNG
CEECCEEEEEEEEHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MWRNKTCRKRNTRQRGFTLIEVLVSIAIFATLSVAAYQVVNQVQRSNELSQERTVRLNEL
CCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRALVMMDSDFRQIALRQTRTNGEEPSKKLLHWADYLLDSDSKGIMFARLGWHNPQQQFP
HHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCC
RGEVTKVGYRIKDERLERVWWRYPDTPAGQEGVVTPLLSDVEELNVRFYDGKQWSNEWSN
CCCHHHHCCEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCHHHHHCC
ELTLPAAISVELTLKDYGKIARTYLTPEGNLQKQASGSEDSGQNNSGGAGNNG
CEECCEEEEEEEEHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]