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The map label for this gene is yaaJ [H]

Identifier: 27363999

GI number: 27363999

Start: 537459

End: 538889

Strand: Reverse

Name: yaaJ [H]

Synonym: VV1_0536

Alternate gene names: 27363999

Gene position: 538889-537459 (Counterclockwise)

Preceding gene: 326423729

Following gene: 27363998

Centisome position: 16.42

GC content: 49.34

Gene sequence:

>1431_bases
ATGACAGACTTGATCAATCTAATGAATGACCTGCTTTGGGGTTCCATTCTTGTTTATTTGCTTGTCGGTGTGGGAATTTA
TTTCACGGTTCGACTCGGCTTCATTCAGTTCCGCCATTTCGGCCACATGTTTTCTGTTCTAAAAAACAGCCGTAAATCGG
ACAACGCTGGTATCTCCTCCTTTCAAGCGCTTTGTACCAGCCTTGCTGCACGTGTAGGCACTGGTAACATGGCCGGTGTG
GCTGTTGCGCTGACCGCCGGTGGCCCTGGTGCGATTTTCTGGATGTGGCTCATCGCCATGTTGGGTATGGCGACATCATT
TGCTGAAAGTACGCTAGCTCAGCTCTACAAAACCAAAGATGACGATGGCAACTATCGTGGTGGTCCTGCTTACTACATGG
AAAAAGGCCTTGGTATGCGTTGGATGGGGGTGCTGTTCTCCATCTTCCTTATCATTGCTTTTGGTCTGGTGTTTAACGCG
GTGCAAGCCAACTCGATCGCCAACGCAATGCAAAATGCCTTTGGTTGGGAAAATGCCTACGTAGGCGTTGTGATTGTGGT
GCTGTCTGGTGTGATTATCTTTGGTGGTATCAAACGTATCGCGCGTACTGCTGAGCTTATCGTTCCAGTGATGGCATTGG
CTTACCTTGCTTTAGCGCTGTTTGTGATGCTCTCTAACTTGGAGAAACTACCTGACGTACTCATGCTGATCTTCAAGAGC
GCTTTTGGCTTGCAAGAAGCGGCTGCGGGTGGTTTGGGCTACGCGATTGCACAAGCGATGATTAATGGTATCAAACGTGG
CCTGTTCTCGAACGAAGCCGGTATGGGTTCCGCGCCAAACGCTGCGGCGTCAGCAACGCCATATCCTCCACACCCAGCTT
CTCAGGGGTACGTGCAAATGCTGGGCGTTTTCATGGATACTATTGTGATCTGTTCAGCGACCGTTGCAATTATTTTGATG
TCGGGCGAGTACGTGCCGCATGGTGAGATCACGGGCATTGAATTGACTCAGTTAGCGTTAAGCTCGCAAGTGGGTAGCTG
GGGTAGCATTTTTGTCGCGGTCGCGATCTTCTTCTTCGCGTTCACCTCCATCATTGCTAACTACTCTTATGCAGAAACCA
ACTTGGTCTTCTTGGAGCACAACCACAAAGCTGGCTTAAATGTTTTCCGTTTGGTCGTGCTTGGTCTTGTGGGCTTTGGT
GCGATTGCGCCACTTCCAGTGGTATGGGCTCTGGCTGACGTATCGATGGGTCTGATGGCCATTGTGAACTTAGTCGCGAT
CCTGCTGCTATCTGGTATCGTGATTAAACTGGCAAAAGACTACAACCGTCAGTTACAAGCGGGTAAGTTGCCAACCTTTA
ATGCCGATGATTTTCCTGAGCTGAAATCTCAACTGGAAGAAGGCATTTGGGACAACAACAAGAAAGACTAA

Upstream 100 bases:

>100_bases
AAAGAAAATAATAGTTCACCAAGCTAATTTGACACTTGGTTAAGCAATCTGGTGGATGGCTCGGAAGCACAGACTGGCGC
AAACTCAATTTGAGGTAACT

Downstream 100 bases:

>100_bases
GGTAGAAATCGAGATTTTCTATCACTGTGATTCGAAAAGCCGTGCAGACAATGCACGGCTTTTTTTGTACCCTAGACAAA
AAGAAAATAAGGGAAAGAAT

Product: sodium/alanine symporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MTDLINLMNDLLWGSILVYLLVGVGIYFTVRLGFIQFRHFGHMFSVLKNSRKSDNAGISSFQALCTSLAARVGTGNMAGV
AVALTAGGPGAIFWMWLIAMLGMATSFAESTLAQLYKTKDDDGNYRGGPAYYMEKGLGMRWMGVLFSIFLIIAFGLVFNA
VQANSIANAMQNAFGWENAYVGVVIVVLSGVIIFGGIKRIARTAELIVPVMALAYLALALFVMLSNLEKLPDVLMLIFKS
AFGLQEAAAGGLGYAIAQAMINGIKRGLFSNEAGMGSAPNAAASATPYPPHPASQGYVQMLGVFMDTIVICSATVAIILM
SGEYVPHGEITGIELTQLALSSQVGSWGSIFVAVAIFFFAFTSIIANYSYAETNLVFLEHNHKAGLNVFRLVVLGLVGFG
AIAPLPVVWALADVSMGLMAIVNLVAILLLSGIVIKLAKDYNRQLQAGKLPTFNADDFPELKSQLEEGIWDNNKKD

Sequences:

>Translated_476_residues
MTDLINLMNDLLWGSILVYLLVGVGIYFTVRLGFIQFRHFGHMFSVLKNSRKSDNAGISSFQALCTSLAARVGTGNMAGV
AVALTAGGPGAIFWMWLIAMLGMATSFAESTLAQLYKTKDDDGNYRGGPAYYMEKGLGMRWMGVLFSIFLIIAFGLVFNA
VQANSIANAMQNAFGWENAYVGVVIVVLSGVIIFGGIKRIARTAELIVPVMALAYLALALFVMLSNLEKLPDVLMLIFKS
AFGLQEAAAGGLGYAIAQAMINGIKRGLFSNEAGMGSAPNAAASATPYPPHPASQGYVQMLGVFMDTIVICSATVAIILM
SGEYVPHGEITGIELTQLALSSQVGSWGSIFVAVAIFFFAFTSIIANYSYAETNLVFLEHNHKAGLNVFRLVVLGLVGFG
AIAPLPVVWALADVSMGLMAIVNLVAILLLSGIVIKLAKDYNRQLQAGKLPTFNADDFPELKSQLEEGIWDNNKKD
>Mature_475_residues
TDLINLMNDLLWGSILVYLLVGVGIYFTVRLGFIQFRHFGHMFSVLKNSRKSDNAGISSFQALCTSLAARVGTGNMAGVA
VALTAGGPGAIFWMWLIAMLGMATSFAESTLAQLYKTKDDDGNYRGGPAYYMEKGLGMRWMGVLFSIFLIIAFGLVFNAV
QANSIANAMQNAFGWENAYVGVVIVVLSGVIIFGGIKRIARTAELIVPVMALAYLALALFVMLSNLEKLPDVLMLIFKSA
FGLQEAAAGGLGYAIAQAMINGIKRGLFSNEAGMGSAPNAAASATPYPPHPASQGYVQMLGVFMDTIVICSATVAIILMS
GEYVPHGEITGIELTQLALSSQVGSWGSIFVAVAIFFFAFTSIIANYSYAETNLVFLEHNHKAGLNVFRLVVLGLVGFGA
IAPLPVVWALADVSMGLMAIVNLVAILLLSGIVIKLAKDYNRQLQAGKLPTFNADDFPELKSQLEEGIWDNNKKD

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=476, Percent_Identity=49.3697478991597, Blast_Score=462, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 50996; Mature: 50864

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TDLINLMNDLLWGSILVYLLVGVGIYFTVRLGFIQFRHFGHMFSVLKNSRKSDNAGISS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
FQALCTSLAARVGTGNMAGVAVALTAGGPGAIFWMWLIAMLGMATSFAESTLAQLYKTKD
HHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DDGNYRGGPAYYMEKGLGMRWMGVLFSIFLIIAFGLVFNAVQANSIANAMQNAFGWENAY
CCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VGVVIVVLSGVIIFGGIKRIARTAELIVPVMALAYLALALFVMLSNLEKLPDVLMLIFKS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGLQEAAAGGLGYAIAQAMINGIKRGLFSNEAGMGSAPNAAASATPYPPHPASQGYVQM
HHCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
LGVFMDTIVICSATVAIILMSGEYVPHGEITGIELTQLALSSQVGSWGSIFVAVAIFFFA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FTSIIANYSYAETNLVFLEHNHKAGLNVFRLVVLGLVGFGAIAPLPVVWALADVSMGLMA
HHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVNLVAILLLSGIVIKLAKDYNRQLQAGKLPTFNADDFPELKSQLEEGIWDNNKKD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503 [H]