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The map label for this gene is proA

Identifier: 27363799

GI number: 27363799

Start: 312922

End: 314172

Strand: Reverse

Name: proA

Synonym: VV1_0325

Alternate gene names: 27363799

Gene position: 314172-312922 (Counterclockwise)

Preceding gene: 326423689

Following gene: 27363798

Centisome position: 9.57

GC content: 49.72

Gene sequence:

>1251_bases
GTGGATTTAATCACATTAGGCAAAGCGGCAAAAGATGCTGCTTTTCAGCTTGCCACCGCTTCTACGGCGCAAAAGAACAA
AGCATTGGCCATCATTGCGGATGAACTTGAAGCGAATGCGGCGGATATTTTGGCGGCAAACAGCAAAGATATCGAACTCG
GTCGACAAGCGGGTTTGAGCGAAGCGATGCTGGATCGTTTACTTTTGAATGAATCTCGCTTGAATGGCATCGCCAACGAT
GTACGTAACGTGATCAGCCTAACGGATCCGGTCGGTAGCGAAATCGACAGTAAAGTGTTGGAAAATGGCATGCAGCTGTC
ACGCCGTCGTGTGCCGCTTGGCGTGGTTGGTGTGATTTACGAAGCGCGCCCAAATGTCACTATCGACATCGCGGCATTGT
GCCTAAAAACCGGCAATGCGAGCATCCTACGTGGCGGAAAAGAAACCTTCTTCTCCAACATGGAGTTGGTGAAGGTGATT
CAATCGGCGTTAGCGAAAGCTGGCTTGCCAGCGGCATCGGTGCAGTACATTGAAAAACCGGATCGTGAGCTGGTCACTCA
ATTGCTGAAACTGGACGATTACGTTGACATGATCATCCCTCGTGGTGGCGCGGGGCTGCACAAAATGTGTAAAGAGAACA
GCACCATCCCTGTGATCATTGGTGGTTTCGGTATCAGCCATATTTTTGTTGATGAAACGGCTGATCTCGCGAAAAGCGTG
GATGTGGTGGAGAATGCAAAAGCTCAGCGTCCTTCCGCTTGTAATGCGCTGGATACCTTACTTGTTCATGAGCGTATCGC
AGAGCAGTTTCTTCCGATGCTGGTGGCAAAACTCAATGGCAAAGTGACTTTTGTTGTTGAGCCAAAAGCCAAAGCGTACA
TGACTAAAGCTGAACAGGTGCGTGATGCGAGTGATGGTGATTTTGATACCGAATGGTTGAGTTACACGCTGGGAGTTAAA
GTGGTTGCGGATGTGCAAGAAGCGATCGACCACATGCGTGAACATAACGCGAGTCACTCTGATGCCATCATGACGAACCA
TCTGCAAAATGCTGAGTTGTTTATCAACTCGGCGGGCTCTGCGGCGGTGTATGTCAACGCATCGACACGCTTTACCGATG
GCGCACAATTTGGTTTGGGCGCAGAAGTGGCGGTGTCTACTCAGAAATTGCACGCACGTGGTCCAATGGGATTAGAAGAG
TTAACCAGTTACAAATGGGTTGGCAAGGCGAATTACCTTTCACGCGCGTAA

Upstream 100 bases:

>100_bases
TTCGATTCTGGGCTATGACTATGGTTCAGAAGTGATTCACCGTGACGACATGGTTGTCATTCAAGAATAGTCGCTCACCA
GAATCAAAGAGGACAACAAA

Downstream 100 bases:

>100_bases
GCGTAATCGAGTTTGAGTTAACATATCCGGTCTAAACCGTTTTGAAAAAGGGGCATCACGCCCCTTTTTGTTTTATTCGG
CCTAGCAATTCCGCTAAACT

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MDLITLGKAAKDAAFQLATASTAQKNKALAIIADELEANAADILAANSKDIELGRQAGLSEAMLDRLLLNESRLNGIAND
VRNVISLTDPVGSEIDSKVLENGMQLSRRRVPLGVVGVIYEARPNVTIDIAALCLKTGNASILRGGKETFFSNMELVKVI
QSALAKAGLPAASVQYIEKPDRELVTQLLKLDDYVDMIIPRGGAGLHKMCKENSTIPVIIGGFGISHIFVDETADLAKSV
DVVENAKAQRPSACNALDTLLVHERIAEQFLPMLVAKLNGKVTFVVEPKAKAYMTKAEQVRDASDGDFDTEWLSYTLGVK
VVADVQEAIDHMREHNASHSDAIMTNHLQNAELFINSAGSAAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEE
LTSYKWVGKANYLSRA

Sequences:

>Translated_416_residues
MDLITLGKAAKDAAFQLATASTAQKNKALAIIADELEANAADILAANSKDIELGRQAGLSEAMLDRLLLNESRLNGIAND
VRNVISLTDPVGSEIDSKVLENGMQLSRRRVPLGVVGVIYEARPNVTIDIAALCLKTGNASILRGGKETFFSNMELVKVI
QSALAKAGLPAASVQYIEKPDRELVTQLLKLDDYVDMIIPRGGAGLHKMCKENSTIPVIIGGFGISHIFVDETADLAKSV
DVVENAKAQRPSACNALDTLLVHERIAEQFLPMLVAKLNGKVTFVVEPKAKAYMTKAEQVRDASDGDFDTEWLSYTLGVK
VVADVQEAIDHMREHNASHSDAIMTNHLQNAELFINSAGSAAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEE
LTSYKWVGKANYLSRA
>Mature_416_residues
MDLITLGKAAKDAAFQLATASTAQKNKALAIIADELEANAADILAANSKDIELGRQAGLSEAMLDRLLLNESRLNGIAND
VRNVISLTDPVGSEIDSKVLENGMQLSRRRVPLGVVGVIYEARPNVTIDIAALCLKTGNASILRGGKETFFSNMELVKVI
QSALAKAGLPAASVQYIEKPDRELVTQLLKLDDYVDMIIPRGGAGLHKMCKENSTIPVIIGGFGISHIFVDETADLAKSV
DVVENAKAQRPSACNALDTLLVHERIAEQFLPMLVAKLNGKVTFVVEPKAKAYMTKAEQVRDASDGDFDTEWLSYTLGVK
VVADVQEAIDHMREHNASHSDAIMTNHLQNAELFINSAGSAAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEE
LTSYKWVGKANYLSRA

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=418, Percent_Identity=37.799043062201, Blast_Score=249, Evalue=5e-66,
Organism=Homo sapiens, GI21361368, Length=418, Percent_Identity=37.799043062201, Blast_Score=248, Evalue=6e-66,
Organism=Escherichia coli, GI1786438, Length=421, Percent_Identity=57.4821852731591, Blast_Score=492, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17569969, Length=402, Percent_Identity=33.8308457711443, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17569967, Length=402, Percent_Identity=33.8308457711443, Blast_Score=220, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324899, Length=419, Percent_Identity=38.1861575178998, Blast_Score=275, Evalue=9e-75,
Organism=Drosophila melanogaster, GI21357643, Length=406, Percent_Identity=33.2512315270936, Blast_Score=233, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_VIBVU (Q8DF94)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759327.1
- ProteinModelPortal:   Q8DF94
- SMR:   Q8DF94
- GeneID:   1177286
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0325
- HOGENOM:   HBG318080
- OMA:   QYPAACN
- ProtClustDB:   PRK00197
- BioCyc:   VVUL216895:VV1_0325-MONOMER
- BRENDA:   1.2.1.41
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 44760; Mature: 44760

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLITLGKAAKDAAFQLATASTAQKNKALAIIADELEANAADILAANSKDIELGRQAGLS
CCEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHEEECCCCCHHHHHHCCHH
EAMLDRLLLNESRLNGIANDVRNVISLTDPVGSEIDSKVLENGMQLSRRRVPLGVVGVIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEE
EARPNVTIDIAALCLKTGNASILRGGKETFFSNMELVKVIQSALAKAGLPAASVQYIEKP
ECCCCCEEEEEHHHHHCCCHHHCCCCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCC
DRELVTQLLKLDDYVDMIIPRGGAGLHKMCKENSTIPVIIGGFGISHIFVDETADLAKSV
CHHHHHHHHHHHHHHHHEECCCCCHHHHHHHCCCCCEEEEECCCEEEEEECCHHHHHHHH
DVVENAKAQRPSACNALDTLLVHERIAEQFLPMLVAKLNGKVTFVVEPKAKAYMTKAEQV
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHH
RDASDGDFDTEWLSYTLGVKVVADVQEAIDHMREHNASHSDAIMTNHLQNAELFINSAGS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCC
AAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEELTSYKWVGKANYLSRA
EEEEEECCCCCCCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure
MDLITLGKAAKDAAFQLATASTAQKNKALAIIADELEANAADILAANSKDIELGRQAGLS
CCEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHEEECCCCCHHHHHHCCHH
EAMLDRLLLNESRLNGIANDVRNVISLTDPVGSEIDSKVLENGMQLSRRRVPLGVVGVIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEE
EARPNVTIDIAALCLKTGNASILRGGKETFFSNMELVKVIQSALAKAGLPAASVQYIEKP
ECCCCCEEEEEHHHHHCCCHHHCCCCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCC
DRELVTQLLKLDDYVDMIIPRGGAGLHKMCKENSTIPVIIGGFGISHIFVDETADLAKSV
CHHHHHHHHHHHHHHHHEECCCCCHHHHHHHCCCCCEEEEECCCEEEEEECCHHHHHHHH
DVVENAKAQRPSACNALDTLLVHERIAEQFLPMLVAKLNGKVTFVVEPKAKAYMTKAEQV
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHH
RDASDGDFDTEWLSYTLGVKVVADVQEAIDHMREHNASHSDAIMTNHLQNAELFINSAGS
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEECCCC
AAVYVNASTRFTDGAQFGLGAEVAVSTQKLHARGPMGLEELTSYKWVGKANYLSRA
EEEEEECCCCCCCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA