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The map label for this gene is proB [H]

Identifier: 326423689

GI number: 326423689

Start: 314412

End: 315341

Strand: Reverse

Name: proB [H]

Synonym: NA

Alternate gene names: 326423689

Gene position: 315341-314412 (Counterclockwise)

Preceding gene: 326423690

Following gene: 27363799

Centisome position: 9.61

GC content: 49.89

Gene sequence:

>930_bases
ATGACAACGAATCAACAAAGCGTCGCAGCTTCTCAACCAAAAACTATTGTTGTGAAGCTTGGAACCAGTGTATTGACCGG
TGGTACATTAGCGTTAGATCGCGCTCATATGGTTGAGCTTGCGCGCCAATGTGCAGAGTTAAAAAAACAAGGCCACTCTG
TGGTGGTCGTCTCGTCAGGCGCGATTGCCGCTGGACGTGAGCACTTAGGTTACCCCGCGCTGCCCAATGCGATGGCAAGC
AAACAGTTGCTTGCTGCGGTTGGGCAAAGTCAGTTGATTCAAACTTGGGAATCGTTATTTGCACTGTATGGCATCAAAAT
TGGCCAAATGTTGCTCACTCGAGCGGATCTCGAAGATCGCGAACGTTTTCTCAATGCTCGAGATACGATTAACGCGTTAG
TGGATAACGGCATTATCCCTGTCGTTAATGAAAATGACGCCGTCGCCACGAGCGAAATCAAAGTGGGTGACAACGACAAC
CTCTCTGCGTTGGTGGGCATTTTATGTGGTGCTGATAAACTACTGTTGCTGACAGACCAAAAAGGCTTGTATACCGCCGA
TCCGCGTAAAGATCCCAATGCTGAGCTGATCAAAGAAGTTAAAGTCATTGACGATACTTTGCGTAAAATTGCTGGTGGCA
GCGGTACAACATTAGGTACTGGTGGGATGGCAACAAAGCTCCAAGCTGCGGACATTGCGCGTCGTGCTGGCATTGAAGTC
ATCATTGCGGCAGGCCGTGGTCAAAATGTCATTTTTGATGCGTTGAGCCCAGCCCCTCAAGGAACCCGTTTCTTGCCTTG
TGAAGAGGCGTTGGAAAACCGCAAGCGTTGGATTCTCGCTGGCCCTGCGGCCTCTGGTGATATTGTGATTGACCAAGGTG
CGGTGAAAGCCGTGGTCGAAAAAGCAGCAGTTTGTTGGCGAAAGGGGTGA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: glutamate 5-kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:
NA

Sequences:

>Translated_309_residues
MTTNQQSVAASQPKTIVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVVVSSGAIAAGREHLGYPALPNAMAS
KQLLAAVGQSQLIQTWESLFALYGIKIGQMLLTRADLEDRERFLNARDTINALVDNGIIPVVNENDAVATSEIKVGDNDN
LSALVGILCGADKLLLLTDQKGLYTADPRKDPNAELIKEVKVIDDTLRKIAGGSGTTLGTGGMATKLQAADIARRAGIEV
IIAAGRGQNVIFDALSPAPQGTRFLPCEEALENRKRWILAGPAASGDIVIDQGAVKAVVEKAAVCWRKG
>Mature_308_residues
TTNQQSVAASQPKTIVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVVVSSGAIAAGREHLGYPALPNAMASK
QLLAAVGQSQLIQTWESLFALYGIKIGQMLLTRADLEDRERFLNARDTINALVDNGIIPVVNENDAVATSEIKVGDNDNL
SALVGILCGADKLLLLTDQKGLYTADPRKDPNAELIKEVKVIDDTLRKIAGGSGTTLGTGGMATKLQAADIARRAGIEVI
IAAGRGQNVIFDALSPAPQGTRFLPCEEALENRKRWILAGPAASGDIVIDQGAVKAVVEKAAVCWRKG

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=286, Percent_Identity=32.1678321678322, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI21361368, Length=288, Percent_Identity=31.9444444444444, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1786437, Length=299, Percent_Identity=67.8929765886288, Blast_Score=403, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17569967, Length=283, Percent_Identity=33.9222614840989, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17569969, Length=283, Percent_Identity=33.9222614840989, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321822, Length=326, Percent_Identity=38.9570552147239, Blast_Score=202, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6320506, Length=331, Percent_Identity=38.9728096676737, Blast_Score=197, Evalue=1e-51,
Organism=Drosophila melanogaster, GI21357643, Length=291, Percent_Identity=32.6460481099656, Blast_Score=124, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 32593; Mature: 32462

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNQQSVAASQPKTIVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVVVSSG
CCCCCCCHHCCCCCEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
AIAAGREHLGYPALPNAMASKQLLAAVGQSQLIQTWESLFALYGIKIGQMLLTRADLEDR
CCCCCHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ERFLNARDTINALVDNGIIPVVNENDAVATSEIKVGDNDNLSALVGILCGADKLLLLTDQ
HHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECC
KGLYTADPRKDPNAELIKEVKVIDDTLRKIAGGSGTTLGTGGMATKLQAADIARRAGIEV
CCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHCCCEE
IIAAGRGQNVIFDALSPAPQGTRFLPCEEALENRKRWILAGPAASGDIVIDQGAVKAVVE
EEECCCCCEEEEECCCCCCCCCEECCHHHHHHCCCEEEEECCCCCCCEEEECHHHHHHHH
KAAVCWRKG
HHHHHHCCC
>Mature Secondary Structure 
TTNQQSVAASQPKTIVVKLGTSVLTGGTLALDRAHMVELARQCAELKKQGHSVVVVSSG
CCCCCCHHCCCCCEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
AIAAGREHLGYPALPNAMASKQLLAAVGQSQLIQTWESLFALYGIKIGQMLLTRADLEDR
CCCCCHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ERFLNARDTINALVDNGIIPVVNENDAVATSEIKVGDNDNLSALVGILCGADKLLLLTDQ
HHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECC
KGLYTADPRKDPNAELIKEVKVIDDTLRKIAGGSGTTLGTGGMATKLQAADIARRAGIEV
CCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHCCCEE
IIAAGRGQNVIFDALSPAPQGTRFLPCEEALENRKRWILAGPAASGDIVIDQGAVKAVVE
EEECCCCCEEEEECCCCCCCCCEECCHHHHHHCCCEEEEECCCCCCCEEEECHHHHHHHH
KAAVCWRKG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA