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The map label for this gene is hemA

Identifier: 27363735

GI number: 27363735

Start: 247138

End: 248397

Strand: Reverse

Name: hemA

Synonym: VV1_0254

Alternate gene names: 27363735

Gene position: 248397-247138 (Counterclockwise)

Preceding gene: 326423678

Following gene: 326423672

Centisome position: 7.57

GC content: 49.76

Gene sequence:

>1260_bases
ATGTCTTTGCTTGCTATTGGCATAAATCACCACACCGCGTCGGTTGACTTGCGAGAGAAAGTGGCGTTTGGTCCAGACAA
ACTGGCTAACGCACTGCAGCAGCTCAGCCAGCATGAAGCAGTCAATGGCAGCGTGATTCTCTCTACCTGTAACCGCACCG
AAGTGTATTGCGATGTCAAATCGGGTGCACGCAGCAAGGTGATTGATTGGTTAAGCCAGTTTCATCAAATCGGTTTAGAA
GAGCTCAAACCGAGTTTGTATGTGTACGAAGAACAAGCAGCAATTCGCCATCTGATGCGCGTTTCTTGCGGCTTGGATTC
TTTGGTGCTGGGTGAACCACAAATATTAGGGCAGGTGAAACAGGCGTATTCCGACTCGCGTGAGCAGCAGGCTGTGGATG
CGTCTTTGGAAAAATTGTTTCAGAAAACCTTTTCCGTTGCTAAGCGCGTCCGCACGGAAACCGACATTGGCGGTAACGCC
GTTTCGGTCGCGTATGCTGCTTGTACGTTAGCCAAACACATTTTTGAATCGTTAGAGAAATCGACGGTTCTATTGGTCGG
TGCTGGTGAAACCATCGAGTTGGTGGCGAAGCACCTTCACGCGAATGGTTGTAGTAAAATGATTGTGGCAAACCGTACTC
GTGAGCGTGCACTGACCTTAGCAGAGCAATTTGATGCTCAGGTGATCAGCTTGCAAGAGATTCCCAATCACCTTGCCAAA
GCGGACATAGTGATCAGCTCCACGGCGAGCCCGCTGCCGATCATAGGGAAAGGCATGGTGGAAACGGCGCTCAAGCAACG
TCGTCATCAGCCTATGTTGTTGGTGGATATTGCAGTCCCGCGTGATGTGGAAGCGCAAGTGGGCGATTTGAATGATGCTT
ACTTGTATACTGTTGACGATCTGCAATCGATTATTGACAGCAATATTGAGCAGCGCAAAGTCGAAGCGATTCAAGCAGAA
GCGATTGTGGCGGAAGAAAGTGCCGCGTTTATGAGTTGGCTTCGCTCGCTCCAAGCGGTGGACAGTATTCGTGAGTATCG
TCAGTCGGCCAATGAAATTCGTGAGGATTTACTCAGCAAAGCGCTGTTATCACTCGAAAGCGGTAGTGACCCAGAAAAAG
TACTGCGTGAGCTGAGTAATCGCCTGACAAATAAACTCATCCATGCACCCACACGTGCGCTACAATTGGCCGCAGAGCAG
GGTGAGCCCGCAAAATTAACCGTCATTCGCCAAAGCTTAGGGCTGGATGAACTCAAATAA

Upstream 100 bases:

>100_bases
GAAGTAAGGAAAACAAATCCGCTACACCCTTTTAATAAAGTCGTGCATAAAGTAAAATTCGCCTCCTAATTTTCCATTCT
GTACAGAGAAAGCACTATCG

Downstream 100 bases:

>100_bases
TTCTATCAATCAGTAAGATATATAAATGATGAAAGCCTCGATTCTAACCAAGCTAGAAATGCTGGTTGAGCGTTATGAAG
AAGTGCAGCACCTTCTTGGT

Product: glutamyl-tRNA reductase

Products: NA

Alternate protein names: GluTR

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MSLLAIGINHHTASVDLREKVAFGPDKLANALQQLSQHEAVNGSVILSTCNRTEVYCDVKSGARSKVIDWLSQFHQIGLE
ELKPSLYVYEEQAAIRHLMRVSCGLDSLVLGEPQILGQVKQAYSDSREQQAVDASLEKLFQKTFSVAKRVRTETDIGGNA
VSVAYAACTLAKHIFESLEKSTVLLVGAGETIELVAKHLHANGCSKMIVANRTRERALTLAEQFDAQVISLQEIPNHLAK
ADIVISSTASPLPIIGKGMVETALKQRRHQPMLLVDIAVPRDVEAQVGDLNDAYLYTVDDLQSIIDSNIEQRKVEAIQAE
AIVAEESAAFMSWLRSLQAVDSIREYRQSANEIREDLLSKALLSLESGSDPEKVLRELSNRLTNKLIHAPTRALQLAAEQ
GEPAKLTVIRQSLGLDELK

Sequences:

>Translated_419_residues
MSLLAIGINHHTASVDLREKVAFGPDKLANALQQLSQHEAVNGSVILSTCNRTEVYCDVKSGARSKVIDWLSQFHQIGLE
ELKPSLYVYEEQAAIRHLMRVSCGLDSLVLGEPQILGQVKQAYSDSREQQAVDASLEKLFQKTFSVAKRVRTETDIGGNA
VSVAYAACTLAKHIFESLEKSTVLLVGAGETIELVAKHLHANGCSKMIVANRTRERALTLAEQFDAQVISLQEIPNHLAK
ADIVISSTASPLPIIGKGMVETALKQRRHQPMLLVDIAVPRDVEAQVGDLNDAYLYTVDDLQSIIDSNIEQRKVEAIQAE
AIVAEESAAFMSWLRSLQAVDSIREYRQSANEIREDLLSKALLSLESGSDPEKVLRELSNRLTNKLIHAPTRALQLAAEQ
GEPAKLTVIRQSLGLDELK
>Mature_418_residues
SLLAIGINHHTASVDLREKVAFGPDKLANALQQLSQHEAVNGSVILSTCNRTEVYCDVKSGARSKVIDWLSQFHQIGLEE
LKPSLYVYEEQAAIRHLMRVSCGLDSLVLGEPQILGQVKQAYSDSREQQAVDASLEKLFQKTFSVAKRVRTETDIGGNAV
SVAYAACTLAKHIFESLEKSTVLLVGAGETIELVAKHLHANGCSKMIVANRTRERALTLAEQFDAQVISLQEIPNHLAKA
DIVISSTASPLPIIGKGMVETALKQRRHQPMLLVDIAVPRDVEAQVGDLNDAYLYTVDDLQSIIDSNIEQRKVEAIQAEA
IVAEESAAFMSWLRSLQAVDSIREYRQSANEIREDLLSKALLSLESGSDPEKVLRELSNRLTNKLIHAPTRALQLAAEQG
EPAKLTVIRQSLGLDELK

Specific function: Catalyzes the NADPH-dependent reduction of glutamyl- tRNA(Glu) to glutamate 1-semialdehyde (GSA)

COG id: COG0373

COG function: function code H; Glutamyl-tRNA reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamyl-tRNA reductase family

Homologues:

Organism=Escherichia coli, GI1787461, Length=418, Percent_Identity=59.8086124401914, Blast_Score=494, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM1_VIBVU (Q8DFF8)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759263.1
- ProteinModelPortal:   Q8DFF8
- GeneID:   1177207
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0254
- HOGENOM:   HBG732626
- OMA:   GPILNRL
- ProtClustDB:   PRK00045
- BioCyc:   VVUL216895:VV1_0254-MONOMER
- BRENDA:   1.2.1.70
- GO:   GO:0005737
- HAMAP:   MF_00087
- InterPro:   IPR000343
- InterPro:   IPR015896
- InterPro:   IPR015895
- InterPro:   IPR016040
- InterPro:   IPR018214
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000445
- TIGRFAMs:   TIGR01035

Pfam domain/function: PF00745 GlutR_dimer; PF05201 GlutR_N; PF01488 Shikimate_DH; SSF69075 4pyrrol_synth_GluRdtase_C; SSF69742 GlutR

EC number: =1.2.1.70

Molecular weight: Translated: 46058; Mature: 45927

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00747 GLUTR

Important sites: ACT_SITE 50-50 BINDING 107-107 BINDING 118-118

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLAIGINHHTASVDLREKVAFGPDKLANALQQLSQHEAVNGSVILSTCNRTEVYCDVK
CCEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECC
SGARSKVIDWLSQFHQIGLEELKPSLYVYEEQAAIRHLMRVSCGLDSLVLGEPQILGQVK
CCCHHHHHHHHHHHHHCCHHHCCCCCEEEHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHH
QAYSDSREQQAVDASLEKLFQKTFSVAKRVRTETDIGGNAVSVAYAACTLAKHIFESLEK
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
STVLLVGAGETIELVAKHLHANGCSKMIVANRTRERALTLAEQFDAQVISLQEIPNHLAK
CEEEEEECCCHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADIVISSTASPLPIIGKGMVETALKQRRHQPMLLVDIAVPRDVEAQVGDLNDAYLYTVDD
HCEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCEEEEHHH
LQSIIDSNIEQRKVEAIQAEAIVAEESAAFMSWLRSLQAVDSIREYRQSANEIREDLLSK
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLSLESGSDPEKVLRELSNRLTNKLIHAPTRALQLAAEQGEPAKLTVIRQSLGLDELK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEHHHHHCCCCCCC
>Mature Secondary Structure 
SLLAIGINHHTASVDLREKVAFGPDKLANALQQLSQHEAVNGSVILSTCNRTEVYCDVK
CEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECC
SGARSKVIDWLSQFHQIGLEELKPSLYVYEEQAAIRHLMRVSCGLDSLVLGEPQILGQVK
CCCHHHHHHHHHHHHHCCHHHCCCCCEEEHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHH
QAYSDSREQQAVDASLEKLFQKTFSVAKRVRTETDIGGNAVSVAYAACTLAKHIFESLEK
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
STVLLVGAGETIELVAKHLHANGCSKMIVANRTRERALTLAEQFDAQVISLQEIPNHLAK
CEEEEEECCCHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADIVISSTASPLPIIGKGMVETALKQRRHQPMLLVDIAVPRDVEAQVGDLNDAYLYTVDD
HCEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCEEEEHHH
LQSIIDSNIEQRKVEAIQAEAIVAEESAAFMSWLRSLQAVDSIREYRQSANEIREDLLSK
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLSLESGSDPEKVLRELSNRLTNKLIHAPTRALQLAAEQGEPAKLTVIRQSLGLDELK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA