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The map label for this gene is ubiF [H]

Identifier: 326423678

GI number: 326423678

Start: 255106

End: 256326

Strand: Reverse

Name: ubiF [H]

Synonym: NA

Alternate gene names: 326423678

Gene position: 256326-255106 (Counterclockwise)

Preceding gene: 27363750

Following gene: 27363735

Centisome position: 7.81

GC content: 47.58

Gene sequence:

>1221_bases
GTGTTTCGCAAACTTGCCAGTAAAACGTACAATGCAAAACCGTCTTTGAATCAAGTTGATCAAGAGTACAAGATGAATAA
ATACGACATTGCCGTCGTCGGTGGCGGCATGGTAGGTGCGGCTGTGGCCATCGGATTTGCCAAACAAGGCAGAAAAGTCA
TCGTGGTTGAAGGGTTTGAGCCGAAAGCGTTTGAAGCAAGCCAACCGATGGACATCCGAGTGTCCGCCATTTCCCACAAC
TCCGTGCGTTTGCTGGAATCGCTAGGGGCCTGGCAACATATTCAAGCAACTCGAGTTTGCCCCTACAAACGGTTAGAAAC
CTGGGAGCACCCAGAATGTCGTACACGTTTTCACAGCGATGAACTCAATTTGGAGCAGCTAGGTTACATTGTGGAAAACC
GTCTCATTCAATTGGGGTTGTGGCAAGAGTTTGCTCAGTACCCGAATTTGACTCTGCAATGCCCAGATAAACTGAAGCGT
CTTGAGTTCGATGAGTTCAACACGTTGACGCTTGAGAGTGGTTTAGAAATTGAAGCCTCTTGGGTCATTGGCGCAGATGG
TGCCAACTCTCAGGTGCGTGAGTTGGCTGGCATTGGCATTACCGCATGGGATTATCGCCAGCATTGTATGTTGATCAATA
TTGCGACGGAATCTGCGCAACAGGCGATCACTTGGCAGCAATTTACCCCAAGTGGCCCACGATCTTTTTTGCCATTGAAA
GGCCAGCAAGGTTCTTTGGTTTGGTACGATTCACCCAAACGTATTCGCGAATTGTCAGCCATGAGCGCAGAAAAGCTGAA
ACAGGAAATTATTCGTCATTTCCCTGATGAATTGGGAGAGTTTGACGTGTTGTACTGGGATTCTTTCCCATTAACCCGAC
GACATGCACAAGCCTATTATCAACAAAATTGTTTGTTGCTTGGGGATGCGGCCCACACGATCAATCCATTAGCCGGCCAA
GGCGTCAATCTTGGTTTTAAAGACGTTGAAGCGTTACTCAAGGCGACGGAAAACCAACCTTTGACAGTGGCTCAACAAGC
GGCCTATCAGAAAGCACGCTATGCGGACAACTTGCTAATGCAAACTGGGATGGATCTGTTCTACAAAGGCTTTAGTAACG
ATATCACCCCATTAAAATTGGTTCGTAACGCAGCCCTCAAGTTGGCTGAGCACTCTGGGCCTATCAAACATCAAGTGTTG
AAATATGCGCTAGGAATGTGA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:
NA

Sequences:

>Translated_406_residues
MFRKLASKTYNAKPSLNQVDQEYKMNKYDIAVVGGGMVGAAVAIGFAKQGRKVIVVEGFEPKAFEASQPMDIRVSAISHN
SVRLLESLGAWQHIQATRVCPYKRLETWEHPECRTRFHSDELNLEQLGYIVENRLIQLGLWQEFAQYPNLTLQCPDKLKR
LEFDEFNTLTLESGLEIEASWVIGADGANSQVRELAGIGITAWDYRQHCMLINIATESAQQAITWQQFTPSGPRSFLPLK
GQQGSLVWYDSPKRIRELSAMSAEKLKQEIIRHFPDELGEFDVLYWDSFPLTRRHAQAYYQQNCLLLGDAAHTINPLAGQ
GVNLGFKDVEALLKATENQPLTVAQQAAYQKARYADNLLMQTGMDLFYKGFSNDITPLKLVRNAALKLAEHSGPIKHQVL
KYALGM
>Mature_406_residues
MFRKLASKTYNAKPSLNQVDQEYKMNKYDIAVVGGGMVGAAVAIGFAKQGRKVIVVEGFEPKAFEASQPMDIRVSAISHN
SVRLLESLGAWQHIQATRVCPYKRLETWEHPECRTRFHSDELNLEQLGYIVENRLIQLGLWQEFAQYPNLTLQCPDKLKR
LEFDEFNTLTLESGLEIEASWVIGADGANSQVRELAGIGITAWDYRQHCMLINIATESAQQAITWQQFTPSGPRSFLPLK
GQQGSLVWYDSPKRIRELSAMSAEKLKQEIIRHFPDELGEFDVLYWDSFPLTRRHAQAYYQQNCLLLGDAAHTINPLAGQ
GVNLGFKDVEALLKATENQPLTVAQQAAYQKARYADNLLMQTGMDLFYKGFSNDITPLKLVRNAALKLAEHSGPIKHQVL
KYALGM

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=420, Percent_Identity=29.0476190476191, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI32967307, Length=337, Percent_Identity=28.1899109792285, Blast_Score=142, Evalue=5e-34,
Organism=Escherichia coli, GI1786883, Length=385, Percent_Identity=51.9480519480519, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1789273, Length=392, Percent_Identity=37.7551020408163, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1789274, Length=394, Percent_Identity=28.1725888324873, Blast_Score=138, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI25146631, Length=431, Percent_Identity=29.0023201856148, Blast_Score=175, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6321694, Length=449, Percent_Identity=23.8307349665924, Blast_Score=99, Evalue=9e-22,
Organism=Drosophila melanogaster, GI19920760, Length=461, Percent_Identity=29.2841648590022, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 45851; Mature: 45851

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRKLASKTYNAKPSLNQVDQEYKMNKYDIAVVGGGMVGAAVAIGFAKQGRKVIVVEGFE
CCHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCC
PKAFEASQPMDIRVSAISHNSVRLLESLGAWQHIQATRVCPYKRLETWEHPECRTRFHSD
CCCCCCCCCCEEEEEEECCCHHHHHHHCCCHHHHHHHHCCCHHHHCCCCCCHHHHCCCCC
ELNLEQLGYIVENRLIQLGLWQEFAQYPNLTLQCPDKLKRLEFDEFNTLTLESGLEIEAS
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCCCCEEEECCCCEEEEE
WVIGADGANSQVRELAGIGITAWDYRQHCMLINIATESAQQAITWQQFTPSGPRSFLPLK
EEEECCCCCHHHHHHHCCCCEEEECCCCEEEEEEECHHHHHHEEHHCCCCCCCCCCCCCC
GQQGSLVWYDSPKRIRELSAMSAEKLKQEIIRHFPDELGEFDVLYWDSFPLTRRHAQAYY
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCEEEEEECCCCCHHHHHHHHH
QQNCLLLGDAAHTINPLAGQGVNLGFKDVEALLKATENQPLTVAQQAAYQKARYADNLLM
HCCCEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
QTGMDLFYKGFSNDITPLKLVRNAALKLAEHSGPIKHQVLKYALGM
HHCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MFRKLASKTYNAKPSLNQVDQEYKMNKYDIAVVGGGMVGAAVAIGFAKQGRKVIVVEGFE
CCHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCC
PKAFEASQPMDIRVSAISHNSVRLLESLGAWQHIQATRVCPYKRLETWEHPECRTRFHSD
CCCCCCCCCCEEEEEEECCCHHHHHHHCCCHHHHHHHHCCCHHHHCCCCCCHHHHCCCCC
ELNLEQLGYIVENRLIQLGLWQEFAQYPNLTLQCPDKLKRLEFDEFNTLTLESGLEIEAS
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHCCCCCCCEEEECCCCEEEEE
WVIGADGANSQVRELAGIGITAWDYRQHCMLINIATESAQQAITWQQFTPSGPRSFLPLK
EEEECCCCCHHHHHHHCCCCEEEECCCCEEEEEEECHHHHHHEEHHCCCCCCCCCCCCCC
GQQGSLVWYDSPKRIRELSAMSAEKLKQEIIRHFPDELGEFDVLYWDSFPLTRRHAQAYY
CCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCEEEEEECCCCCHHHHHHHHH
QQNCLLLGDAAHTINPLAGQGVNLGFKDVEALLKATENQPLTVAQQAAYQKARYADNLLM
HCCCEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
QTGMDLFYKGFSNDITPLKLVRNAALKLAEHSGPIKHQVLKYALGM
HHCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]