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The map label for this gene is rho [H]

Identifier: 23502912

GI number: 23502912

Start: 1990651

End: 1991916

Strand: Reverse

Name: rho [H]

Synonym: BR2064

Alternate gene names: 23502912

Gene position: 1991916-1990651 (Counterclockwise)

Preceding gene: 23502913

Following gene: 23502910

Centisome position: 94.5

GC content: 55.29

Gene sequence:

>1266_bases
ATGCAGGAAATGAAACTACAAGAACTGAAAAACAAGACACCGGTGGAGCTTCTGGCCTTTGCCGAGACGCTTGAGGTCGA
AAATGCGAGCGCCATGCGCAAGCAGGAACTGATGTTCGCGATCCTCAAGAAGCTGGCAGCCCAGGATGTCGAAATCATCG
GTGAGGGCGTTGTCGAGGTGCTACAGGACGGTTTTGGCTTCCTGCGCTCAGCCGATGCCAATTACCTACCCGGTCCGGAC
GATATCTATATTTCGCCATCGCAACTGCGCCGTTTTTCGCTCAAGACCGGCGATACGGTTGAAGGCCCGATCCGCGGGCC
CAAGGAAGGCGAGCGCTATTTTGCGCTGCTCAAGGTCAATACGATCAATTTTGAAGATCCGGAAAAGATCCGTCACAAGG
TTCACTTCGACAATCTGACGCCGCTCTACCCAAACGAGCGTTTCAAGATGGAGCTGGAAGTTCCGACCTCGAAAGATCTT
TCGCCGCGTGTCATAGATCTGGTTGCGCCGCTCGGCAAAGGCCAGCGCGGCCTGATCGTCGCGCCGCCGCGCACCGGTAA
GACTGTTCTTCTCCAGAACATCGCCCATTCGATCACTGCAAATCACCCGGAATGCTATCTTATCGTTCTCCTGATCGATG
AGCGTCCGGAAGAAGTGACCGACATGCAGCGTTCGGTGAAGGGTGAGGTTATTTCCTCGACCTTCGATGAACCGGCCGCC
CGCCATGTTCAGGTTGCTGAAATGGTCATTGAAAAGGCAAAACGCCTTGTCGAACATGGCCGTGACGTCGTTATCCTGCT
CGACTCGATCACGCGCCTTGGCCGCGCCTACAATACGGTCGTGCCGTCTTCCGGCAAGGTGCTGACCGGTGGTGTGGACG
CCAATGCGCTACAGCGCCCGAAGCGCTTCTTTGGTGCTGCGCGTAACATCGAGGAAGGCGGCTCGCTTACCATTATCGCA
ACTGCCCTGATCGATACCGGCAGCCGCATGGACGAAGTGATCTTTGAAGAATTCAAAGGCACGGGTAACTCGGAAATCGT
TCTCGACCGCAAGGTTGCCGACAAGCGCATCTTCCCGGCTATGGATATTCTCAAGTCCGGTACGCGCAAGGAAGATCTGC
TGGTTCCGCGCGCCGATCTCCAGAAGATTTTCGTGCTGCGCCGCATTCTTGCACCCATGGGCACGACCGATGCGATCGAA
TTCCTCATCGACAAGCTCAAGCAGACGAAGAGCAATGCCGAATTCTTCGATTCGATGAATACGTAA

Upstream 100 bases:

>100_bases
CCCTTCCTGACAGCAGGCCTTCGATGCCGGTCGCGATCCAAGTGACCACCTTTCCGAACAACTGCATTTTCTCCTTATAT
TTAAAAAGAGTTCCTCATCC

Downstream 100 bases:

>100_bases
TCGACGCTACCCATTCATTTCAAACAACGCTGCGTGGGAAACCGCGCGGCGTTTATTTTGATTGGTGGTGCACTTCTTTA
TGGCTCAGAGAAGTGAGCGC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MQEMKLQELKNKTPVELLAFAETLEVENASAMRKQELMFAILKKLAAQDVEIIGEGVVEVLQDGFGFLRSADANYLPGPD
DIYISPSQLRRFSLKTGDTVEGPIRGPKEGERYFALLKVNTINFEDPEKIRHKVHFDNLTPLYPNERFKMELEVPTSKDL
SPRVIDLVAPLGKGQRGLIVAPPRTGKTVLLQNIAHSITANHPECYLIVLLIDERPEEVTDMQRSVKGEVISSTFDEPAA
RHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIA
TALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPAMDILKSGTRKEDLLVPRADLQKIFVLRRILAPMGTTDAIE
FLIDKLKQTKSNAEFFDSMNT

Sequences:

>Translated_421_residues
MQEMKLQELKNKTPVELLAFAETLEVENASAMRKQELMFAILKKLAAQDVEIIGEGVVEVLQDGFGFLRSADANYLPGPD
DIYISPSQLRRFSLKTGDTVEGPIRGPKEGERYFALLKVNTINFEDPEKIRHKVHFDNLTPLYPNERFKMELEVPTSKDL
SPRVIDLVAPLGKGQRGLIVAPPRTGKTVLLQNIAHSITANHPECYLIVLLIDERPEEVTDMQRSVKGEVISSTFDEPAA
RHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIA
TALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPAMDILKSGTRKEDLLVPRADLQKIFVLRRILAPMGTTDAIE
FLIDKLKQTKSNAEFFDSMNT
>Mature_421_residues
MQEMKLQELKNKTPVELLAFAETLEVENASAMRKQELMFAILKKLAAQDVEIIGEGVVEVLQDGFGFLRSADANYLPGPD
DIYISPSQLRRFSLKTGDTVEGPIRGPKEGERYFALLKVNTINFEDPEKIRHKVHFDNLTPLYPNERFKMELEVPTSKDL
SPRVIDLVAPLGKGQRGLIVAPPRTGKTVLLQNIAHSITANHPECYLIVLLIDERPEEVTDMQRSVKGEVISSTFDEPAA
RHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIA
TALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPAMDILKSGTRKEDLLVPRADLQKIFVLRRILAPMGTTDAIE
FLIDKLKQTKSNAEFFDSMNT

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=69.2307692307692, Blast_Score=610, Evalue=1e-176,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 46965; Mature: 46965

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEMKLQELKNKTPVELLAFAETLEVENASAMRKQELMFAILKKLAAQDVEIIGEGVVEV
CCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQDGFGFLRSADANYLPGPDDIYISPSQLRRFSLKTGDTVEGPIRGPKEGERYFALLKVN
HHHHHHHHHCCCCCCCCCCCCEEECHHHHHHEECCCCCCCCCCCCCCCCCCEEEEEEEEE
TINFEDPEKIRHKVHFDNLTPLYPNERFKMELEVPTSKDLSPRVIDLVAPLGKGQRGLIV
EECCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCEEE
APPRTGKTVLLQNIAHSITANHPECYLIVLLIDERPEEVTDMQRSVKGEVISSTFDEPAA
ECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHH
RHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRP
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHH
KRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPA
HHHHHHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHH
MDILKSGTRKEDLLVPRADLQKIFVLRRILAPMGTTDAIEFLIDKLKQTKSNAEFFDSMN
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHCCC
T
C
>Mature Secondary Structure
MQEMKLQELKNKTPVELLAFAETLEVENASAMRKQELMFAILKKLAAQDVEIIGEGVVEV
CCCCHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQDGFGFLRSADANYLPGPDDIYISPSQLRRFSLKTGDTVEGPIRGPKEGERYFALLKVN
HHHHHHHHHCCCCCCCCCCCCEEECHHHHHHEECCCCCCCCCCCCCCCCCCEEEEEEEEE
TINFEDPEKIRHKVHFDNLTPLYPNERFKMELEVPTSKDLSPRVIDLVAPLGKGQRGLIV
EECCCCHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCEEE
APPRTGKTVLLQNIAHSITANHPECYLIVLLIDERPEEVTDMQRSVKGEVISSTFDEPAA
ECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHH
RHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRP
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHH
KRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPA
HHHHHHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHH
MDILKSGTRKEDLLVPRADLQKIFVLRRILAPMGTTDAIEFLIDKLKQTKSNAEFFDSMN
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606169 [H]