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The map label for this gene is trmE [H]

Identifier: 23502910

GI number: 23502910

Start: 1987123

End: 1988451

Strand: Reverse

Name: trmE [H]

Synonym: BR2062

Alternate gene names: 23502910

Gene position: 1988451-1987123 (Counterclockwise)

Preceding gene: 23502912

Following gene: 23502909

Centisome position: 94.34

GC content: 52.75

Gene sequence:

>1329_bases
ATGAGCGAGATCGGTTCTTATCACGATACGATTTTTGCATTATCGAGCGGACGCTTGCCCTCAGGGGTCGCGGTCATTCG
CATTTCCGGCCCAAAAACTCGATTCGTTTACGAAACAATCTGCCAAGCAATTCCGGAACCGCGCCATGCCGCATTGCTTA
CATTCCGTAGCCGAAATGGTGACGCCATTGATCGCGGCCTCACTCTGTTTTTCCCTGCTCCTCATAGTTTTACTGGGGAA
GACTGCGCCGAATTTCATTTGCACGGCGGTAAGGCCGTTGTGGAAAAGATGCTTGCGGTGTTGGGCGAACTGCCGGGATG
CCGAATTGCGGAGGCTGGTGAATTTACGCGACGTGCTTTTGCCAATGGCAAGATGGACTTGACGATTGCAGAAGGTCTGG
CCGACCTCATTGCTGCGGAAACCGAGGGGCAGCGACGGCTTGCAATGCAAGTGGCTTCCGGTAATCAGCGCAAACTATAT
AGTGAGTGGCGCCAGCGACTTATCAATGCGCGGGCTTTCATTGAAGCTGAACTGGATTTTGCCGATGAAAGCGACGTACC
CGGTTCTGTTTCCATGCAGGTCTGGCAACAGCTTTCGGCGCTCAAACATGAAATCGAGCACCATATCGCAAGCGGTAAGC
GCGCTGCGATGCTGCGGGATGGATTGCATGTTGTTATTGTCGGCGCGCCAAATGCCGGTAAATCCAGCTTGCTTAATTTT
CTAGCCGGGCGCGATGTTGCGATCATTTCCAAAGAAGCTGGTACGACGCGCGATCTTCTGGAAGTGAAACTCGATCTCGG
CGGTATCCCGGTTTATGTCACCGATACGGCGGGTCTTCGCGAAACGGATAGTGTCGTTGAGAAGATCGGCATTGAGCGTG
CGCGTGCCCGGATGGCCGAAGCCGATCTGGTGCTCTCGCTGGAAGATATGAGCGGACCTGTATCTGTTACCGTTGAGAAA
ATCGAAGCAGAAACCTGGCTGATCGGTACCAAGGCCGACCTCGGTGGAAGTGCTTCGGGCTTGTGGAAATATCACATTTC
CACCATGACTGGCAGTGGTCTGGAACAATTGCTGGACGCTCTACAGGCTTTTGCTGAAGCGAAGATCGGCCAGATTGAAG
ATGCTGTTCCTACCCGGCAGCGCCATATCAACCTGTTGCGGGCAACGATTGAAGAAATCGAAAAAGCAATTGAGGGTGAC
GATCTTCCATTGGAGTTAAGAGCCGAGAATATGCGGCTTGCTTCGCAATTCCTTGGACGGATCACGGGCGATGTGGATGT
AGAGGAAATTCTGGACGTTATTTTCTCGCAATTCTGCATCGGGAAGTGA

Upstream 100 bases:

>100_bases
GGGTCCCGAATGAATATTCACAAGTCGCCTCCAAGCGAATACTGGGGCGAGGATACGGGGGCATTGAGCAGTTCCCCATG
ATTATAGGTCAGGAAACAGA

Downstream 100 bases:

>100_bases
TTCACGTGAAACATGCAGCTTTATGAGCACCAATAGCCGTTGCAATGTTTCACGTGAAACATCTGTGGAATCCTGCTTGT
TGAATTGACTCCCATCAATG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSEIGSYHDTIFALSSGRLPSGVAVIRISGPKTRFVYETICQAIPEPRHAALLTFRSRNGDAIDRGLTLFFPAPHSFTGE
DCAEFHLHGGKAVVEKMLAVLGELPGCRIAEAGEFTRRAFANGKMDLTIAEGLADLIAAETEGQRRLAMQVASGNQRKLY
SEWRQRLINARAFIEAELDFADESDVPGSVSMQVWQQLSALKHEIEHHIASGKRAAMLRDGLHVVIVGAPNAGKSSLLNF
LAGRDVAIISKEAGTTRDLLEVKLDLGGIPVYVTDTAGLRETDSVVEKIGIERARARMAEADLVLSLEDMSGPVSVTVEK
IEAETWLIGTKADLGGSASGLWKYHISTMTGSGLEQLLDALQAFAEAKIGQIEDAVPTRQRHINLLRATIEEIEKAIEGD
DLPLELRAENMRLASQFLGRITGDVDVEEILDVIFSQFCIGK

Sequences:

>Translated_442_residues
MSEIGSYHDTIFALSSGRLPSGVAVIRISGPKTRFVYETICQAIPEPRHAALLTFRSRNGDAIDRGLTLFFPAPHSFTGE
DCAEFHLHGGKAVVEKMLAVLGELPGCRIAEAGEFTRRAFANGKMDLTIAEGLADLIAAETEGQRRLAMQVASGNQRKLY
SEWRQRLINARAFIEAELDFADESDVPGSVSMQVWQQLSALKHEIEHHIASGKRAAMLRDGLHVVIVGAPNAGKSSLLNF
LAGRDVAIISKEAGTTRDLLEVKLDLGGIPVYVTDTAGLRETDSVVEKIGIERARARMAEADLVLSLEDMSGPVSVTVEK
IEAETWLIGTKADLGGSASGLWKYHISTMTGSGLEQLLDALQAFAEAKIGQIEDAVPTRQRHINLLRATIEEIEKAIEGD
DLPLELRAENMRLASQFLGRITGDVDVEEILDVIFSQFCIGK
>Mature_441_residues
SEIGSYHDTIFALSSGRLPSGVAVIRISGPKTRFVYETICQAIPEPRHAALLTFRSRNGDAIDRGLTLFFPAPHSFTGED
CAEFHLHGGKAVVEKMLAVLGELPGCRIAEAGEFTRRAFANGKMDLTIAEGLADLIAAETEGQRRLAMQVASGNQRKLYS
EWRQRLINARAFIEAELDFADESDVPGSVSMQVWQQLSALKHEIEHHIASGKRAAMLRDGLHVVIVGAPNAGKSSLLNFL
AGRDVAIISKEAGTTRDLLEVKLDLGGIPVYVTDTAGLRETDSVVEKIGIERARARMAEADLVLSLEDMSGPVSVTVEKI
EAETWLIGTKADLGGSASGLWKYHISTMTGSGLEQLLDALQAFAEAKIGQIEDAVPTRQRHINLLRATIEEIEKAIEGDD
LPLELRAENMRLASQFLGRITGDVDVEEILDVIFSQFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=460, Percent_Identity=43.9130434782609, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI193082988, Length=492, Percent_Identity=41.260162601626, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI193082993, Length=444, Percent_Identity=43.6936936936937, Blast_Score=316, Evalue=3e-86,
Organism=Escherichia coli, GI2367268, Length=462, Percent_Identity=37.8787878787879, Blast_Score=271, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI17507259, Length=452, Percent_Identity=33.6283185840708, Blast_Score=220, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6323665, Length=490, Percent_Identity=33.6734693877551, Blast_Score=249, Evalue=7e-67,
Organism=Drosophila melanogaster, GI45550813, Length=471, Percent_Identity=39.7027600849257, Blast_Score=305, Evalue=5e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 48085; Mature: 47953

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIGSYHDTIFALSSGRLPSGVAVIRISGPKTRFVYETICQAIPEPRHAALLTFRSRNG
CCCCCCHHHEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCC
DAIDRGLTLFFPAPHSFTGEDCAEFHLHGGKAVVEKMLAVLGELPGCRIAEAGEFTRRAF
CHHHCCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
ANGKMDLTIAEGLADLIAAETEGQRRLAMQVASGNQRKLYSEWRQRLINARAFIEAELDF
CCCCEEEEHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCC
ADESDVPGSVSMQVWQQLSALKHEIEHHIASGKRAAMLRDGLHVVIVGAPNAGKSSLLNF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCHHHHHHH
LAGRDVAIISKEAGTTRDLLEVKLDLGGIPVYVTDTAGLRETDSVVEKIGIERARARMAE
HCCCCEEEEECCCCCHHHHHHEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
ADLVLSLEDMSGPVSVTVEKIEAETWLIGTKADLGGSASGLWKYHISTMTGSGLEQLLDA
HHEEEEEECCCCCEEEEEEEECCCEEEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHH
LQAFAEAKIGQIEDAVPTRQRHINLLRATIEEIEKAIEGDDLPLELRAENMRLASQFLGR
HHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHH
ITGDVDVEEILDVIFSQFCIGK
HCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEIGSYHDTIFALSSGRLPSGVAVIRISGPKTRFVYETICQAIPEPRHAALLTFRSRNG
CCCCCHHHEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCCC
DAIDRGLTLFFPAPHSFTGEDCAEFHLHGGKAVVEKMLAVLGELPGCRIAEAGEFTRRAF
CHHHCCEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
ANGKMDLTIAEGLADLIAAETEGQRRLAMQVASGNQRKLYSEWRQRLINARAFIEAELDF
CCCCEEEEHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCC
ADESDVPGSVSMQVWQQLSALKHEIEHHIASGKRAAMLRDGLHVVIVGAPNAGKSSLLNF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCHHHHHHH
LAGRDVAIISKEAGTTRDLLEVKLDLGGIPVYVTDTAGLRETDSVVEKIGIERARARMAE
HCCCCEEEEECCCCCHHHHHHEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
ADLVLSLEDMSGPVSVTVEKIEAETWLIGTKADLGGSASGLWKYHISTMTGSGLEQLLDA
HHEEEEEECCCCCEEEEEEEECCCEEEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHH
LQAFAEAKIGQIEDAVPTRQRHINLLRATIEEIEKAIEGDDLPLELRAENMRLASQFLGR
HHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHH
ITGDVDVEEILDVIFSQFCIGK
HCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA