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The map label for this gene is ruvA

Identifier: 23502561

GI number: 23502561

Start: 1646722

End: 1647339

Strand: Reverse

Name: ruvA

Synonym: BR1703

Alternate gene names: 23502561

Gene position: 1647339-1646722 (Counterclockwise)

Preceding gene: 23502562

Following gene: 23502560

Centisome position: 78.15

GC content: 61.33

Gene sequence:

>618_bases
ATGATCGGCAAGTTGAAGGGCGTGATTGACGAAATTGCCGAGGACCATGCTGTCATCGACGTGCATGGCGTGGGCTATGT
CGCGTTTTGTTCGGCGCGCACGCTGGGCAATCTCGGTGGCGCGGGCGAGGCGGCCATCCTGTTCATCGAAACCTATGTCC
GTGAAGATATGATCCGCCTTTATGGATTTGCCACGCAGCTTGAGCGCGAATGGTTCCGCCTGTTGCAGAATGTGCAAGGG
GTGGGGGCCAAGGTGGCTTTGGCGGTGCTTGGCACCCTGTCGCCGTCCGAACTTGCCAATGCGATTGCGCTGCGCGACAT
TGCCATGGTTTCGCGCGCGCCGGGCGTCGGCAAGAAAGTGGCAGAGCGTATCGTTACCGAATTGAAGAACAAGGCTCCCG
CCTTTGCCGGTGAGGCGAGCGGCACGATCGGGCTCAAGCAGGAGCTTGGCGCAGGTGCGGCGCCTGCACCCGTTGCCGAT
GCGGTTTCGGCGCTTTCCAACCTCGGTTATTCGCGCGATCAGGCCGCCAATGCCGTGGCAGCCGCCTTGAAGGAAACGGG
CGAGGGCGCCGATTCGGCCAAGCTGATACGGCTCGGTTTGAAGGAACTCTCGCAGTGA

Upstream 100 bases:

>100_bases
GATGCCGCCGATGCGCTGGCCATCGCCATATGTCATGCGCATCACCGGCAGAGCATCGTGAGTGCCCGCCGGATGCAGGC
ATTGTTAGCGGGGTAATATC

Downstream 100 bases:

>100_bases
TTTACTTGTCTGAACATGAGCTTCATGCGAGGAGAGGGGCATGAGCGACCGTAACCCCCTGATCGATGCCGACCGGCGCG
CAGACGAAGACAATACGCTG

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MIGKLKGVIDEIAEDHAVIDVHGVGYVAFCSARTLGNLGGAGEAAILFIETYVREDMIRLYGFATQLEREWFRLLQNVQG
VGAKVALAVLGTLSPSELANAIALRDIAMVSRAPGVGKKVAERIVTELKNKAPAFAGEASGTIGLKQELGAGAAPAPVAD
AVSALSNLGYSRDQAANAVAAALKETGEGADSAKLIRLGLKELSQ

Sequences:

>Translated_205_residues
MIGKLKGVIDEIAEDHAVIDVHGVGYVAFCSARTLGNLGGAGEAAILFIETYVREDMIRLYGFATQLEREWFRLLQNVQG
VGAKVALAVLGTLSPSELANAIALRDIAMVSRAPGVGKKVAERIVTELKNKAPAFAGEASGTIGLKQELGAGAAPAPVAD
AVSALSNLGYSRDQAANAVAAALKETGEGADSAKLIRLGLKELSQ
>Mature_205_residues
MIGKLKGVIDEIAEDHAVIDVHGVGYVAFCSARTLGNLGGAGEAAILFIETYVREDMIRLYGFATQLEREWFRLLQNVQG
VGAKVALAVLGTLSPSELANAIALRDIAMVSRAPGVGKKVAERIVTELKNKAPAFAGEASGTIGLKQELGAGAAPAPVAD
AVSALSNLGYSRDQAANAVAAALKETGEGADSAKLIRLGLKELSQ

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=204, Percent_Identity=36.2745098039216, Blast_Score=110, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_BRUA1 (B2S7E0)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935561.1
- ProteinModelPortal:   B2S7E0
- SMR:   B2S7E0
- GeneID:   6327551
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I16030
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- ProtClustDB:   PRK00116
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 21228; Mature: 21228

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGKLKGVIDEIAEDHAVIDVHGVGYVAFCSARTLGNLGGAGEAAILFIETYVREDMIRL
CCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
YGFATQLEREWFRLLQNVQGVGAKVALAVLGTLSPSELANAIALRDIAMVSRAPGVGKKV
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
AERIVTELKNKAPAFAGEASGTIGLKQELGAGAAPAPVADAVSALSNLGYSRDQAANAVA
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
AALKETGEGADSAKLIRLGLKELSQ
HHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIGKLKGVIDEIAEDHAVIDVHGVGYVAFCSARTLGNLGGAGEAAILFIETYVREDMIRL
CCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
YGFATQLEREWFRLLQNVQGVGAKVALAVLGTLSPSELANAIALRDIAMVSRAPGVGKKV
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
AERIVTELKNKAPAFAGEASGTIGLKQELGAGAAPAPVADAVSALSNLGYSRDQAANAVA
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
AALKETGEGADSAKLIRLGLKELSQ
HHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA