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The map label for this gene is cca [H]

Identifier: 23502417

GI number: 23502417

Start: 1501915

End: 1503186

Strand: Reverse

Name: cca [H]

Synonym: BR1553

Alternate gene names: 23502417

Gene position: 1503186-1501915 (Counterclockwise)

Preceding gene: 23502418

Following gene: 23502416

Centisome position: 71.32

GC content: 59.2

Gene sequence:

>1272_bases
TTGAGCGAGAGTATAAATATTTCCGGCAAGGCAGATTGGCTGAATGCGAAGCCGTTGCAGGCGCTTTTCACGGCATTGAA
CCGCGATGGCGGCGAGGCGCGCGTGGTTGGCGGCGCGGTGCGCAATACACTCCTCGGCACCGGGGTGAGCGACGTCGATC
TGGCGACGACGCATCTGCCGGAAGAAACCGTGCGGCTGGCAAGTGAAGCCGGTTTCAAGCCGGTGCCGACAGGCATGGAA
CACGGTACGATCACCGTCGTCGTGCAAGGATACCCCTTTGAAGTGACGACACTGAGGCGGGATATCGAAACGAATGGTCG
CCATGCGAAAGTCGCCTTCGGAACCGACTGGAAAGCAGATGCCGAACGGCGCGATTTCACCATTAATGCGCTTTATGTCA
CTGCGGACGGTACGGTGATCGATCAGGTGGGCGGGCTTGCCGATATAGAGACGCGGACATTGCGTTTCATCGGCGATGCG
GAACAGCGCATTCGCGAGGATTATCTGCGCATCCTGCGTTTTTTCCGCTTCTTTGCCTGGTACGGTTCAGGCCGGCCCGA
AGCGGAAGGGCTGCGCGCCAGCGCAAGGCTGAAGGACGGTATTGCGCAGCTTTCCGCCGAACGCATCTGGTCGGAAATGA
AGAAGCTGCTCGGTGCTCCCGATCCCTCGCGCGCGCTTTTGTGGATGCGGCAGGCCGGCGTGCTCAATCTCGTTCTGCCT
GAAAGCGAGAAATGGGGCATTGATGCCATTCATGGGCTGGTGCGCACGCAAAGCGATCTTGGATGGCAGGCCGATCCGCT
CTTGCGGCTGGAAAGTCTTGTTCCGCCCGATGCTGTCCGTATGGAAGAGCTGGGCAAGCGTATGAAAATGTCCAACGCCG
AAAGGGCGCGTCTGGAAGCATGGGCGCGGGCCGATGCGGTGAAGCCGGGGCTTTCCGAACAAGCACTGAAGAAAGCGATT
TATCGCGGCAGCAAGCAGGCTTTGCTGGATCGTATTCGTCTGGCCTATGCGGCGGCGCGTCAGGAGGCCGTGGGCAGCGA
TGAGGCGATGATCCGCGCCGGTGGTGTCGCACGGCTTCTGGATGCCGCCGAACATTACGATGCCCCTGTTTTCCCCATTA
CGGGCGGTGATCTTCTGGCGCGCGGCATTGAAAAGGGGCCGGGGCTTGGAGAAACATTACGGTCTCTTGAAACTCTCTGG
ATTGACTCCTGTTTTAGCCTTGACCGGGAGGCGCTTCTGCGCAAGCTTGATCGTGATAATTTGAACGACTGA

Upstream 100 bases:

>100_bases
ACGGCAAGGAACGCTTCTATTACGCCATGCCCTATCACGAGCGATTCATCTGGGGGGCAACCGCCGGCATCATTCGCGGA
CTTTATGAAAGGCTCTACGT

Downstream 100 bases:

>100_bases
TGGCTTGCTTGCAAATTTCAGCCGTAGTTCCATCGGCTTATGGCTGGGGCAAAACAGGCCCGAAACAGGAAATGAAAAGT
TTTTAGCGCGCGTTTTGAAA

Product: polyA polymerase family protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSESINISGKADWLNAKPLQALFTALNRDGGEARVVGGAVRNTLLGTGVSDVDLATTHLPEETVRLASEAGFKPVPTGME
HGTITVVVQGYPFEVTTLRRDIETNGRHAKVAFGTDWKADAERRDFTINALYVTADGTVIDQVGGLADIETRTLRFIGDA
EQRIREDYLRILRFFRFFAWYGSGRPEAEGLRASARLKDGIAQLSAERIWSEMKKLLGAPDPSRALLWMRQAGVLNLVLP
ESEKWGIDAIHGLVRTQSDLGWQADPLLRLESLVPPDAVRMEELGKRMKMSNAERARLEAWARADAVKPGLSEQALKKAI
YRGSKQALLDRIRLAYAAARQEAVGSDEAMIRAGGVARLLDAAEHYDAPVFPITGGDLLARGIEKGPGLGETLRSLETLW
IDSCFSLDREALLRKLDRDNLND

Sequences:

>Translated_423_residues
MSESINISGKADWLNAKPLQALFTALNRDGGEARVVGGAVRNTLLGTGVSDVDLATTHLPEETVRLASEAGFKPVPTGME
HGTITVVVQGYPFEVTTLRRDIETNGRHAKVAFGTDWKADAERRDFTINALYVTADGTVIDQVGGLADIETRTLRFIGDA
EQRIREDYLRILRFFRFFAWYGSGRPEAEGLRASARLKDGIAQLSAERIWSEMKKLLGAPDPSRALLWMRQAGVLNLVLP
ESEKWGIDAIHGLVRTQSDLGWQADPLLRLESLVPPDAVRMEELGKRMKMSNAERARLEAWARADAVKPGLSEQALKKAI
YRGSKQALLDRIRLAYAAARQEAVGSDEAMIRAGGVARLLDAAEHYDAPVFPITGGDLLARGIEKGPGLGETLRSLETLW
IDSCFSLDREALLRKLDRDNLND
>Mature_422_residues
SESINISGKADWLNAKPLQALFTALNRDGGEARVVGGAVRNTLLGTGVSDVDLATTHLPEETVRLASEAGFKPVPTGMEH
GTITVVVQGYPFEVTTLRRDIETNGRHAKVAFGTDWKADAERRDFTINALYVTADGTVIDQVGGLADIETRTLRFIGDAE
QRIREDYLRILRFFRFFAWYGSGRPEAEGLRASARLKDGIAQLSAERIWSEMKKLLGAPDPSRALLWMRQAGVLNLVLPE
SEKWGIDAIHGLVRTQSDLGWQADPLLRLESLVPPDAVRMEELGKRMKMSNAERARLEAWARADAVKPGLSEQALKKAIY
RGSKQALLDRIRLAYAAARQEAVGSDEAMIRAGGVARLLDAAEHYDAPVFPITGGDLLARGIEKGPGLGETLRSLETLWI
DSCFSLDREALLRKLDRDNLND

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=422, Percent_Identity=31.5165876777251, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI87081691, Length=208, Percent_Identity=36.0576923076923, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789436, Length=224, Percent_Identity=30.8035714285714, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=406, Percent_Identity=34.2364532019704, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6321016, Length=229, Percent_Identity=32.3144104803493, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281360112, Length=406, Percent_Identity=33.2512315270936, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI21357337, Length=406, Percent_Identity=33.2512315270936, Blast_Score=189, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46600; Mature: 46468

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESINISGKADWLNAKPLQALFTALNRDGGEARVVGGAVRNTLLGTGVSDVDLATTHLP
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCCCCCCHHHHCCC
EETVRLASEAGFKPVPTGMEHGTITVVVQGYPFEVTTLRRDIETNGRHAKVAFGTDWKAD
HHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEHHHHHHHHCCCCCEEEEEECCCCCCC
AERRDFTINALYVTADGTVIDQVGGLADIETRTLRFIGDAEQRIREDYLRILRFFRFFAW
CCCCCEEEEEEEEEECCCHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
YGSGRPEAEGLRASARLKDGIAQLSAERIWSEMKKLLGAPDPSRALLWMRQAGVLNLVLP
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEEC
ESEKWGIDAIHGLVRTQSDLGWQADPLLRLESLVPPDAVRMEELGKRMKMSNAERARLEA
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHH
WARADAVKPGLSEQALKKAIYRGSKQALLDRIRLAYAAARQEAVGSDEAMIRAGGVARLL
HHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHH
DAAEHYDAPVFPITGGDLLARGIEKGPGLGETLRSLETLWIDSCFSLDREALLRKLDRDN
HHHHHCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
LND
CCC
>Mature Secondary Structure 
SESINISGKADWLNAKPLQALFTALNRDGGEARVVGGAVRNTLLGTGVSDVDLATTHLP
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCCCCCCHHHHCCC
EETVRLASEAGFKPVPTGMEHGTITVVVQGYPFEVTTLRRDIETNGRHAKVAFGTDWKAD
HHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEEHHHHHHHHCCCCCEEEEEECCCCCCC
AERRDFTINALYVTADGTVIDQVGGLADIETRTLRFIGDAEQRIREDYLRILRFFRFFAW
CCCCCEEEEEEEEEECCCHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
YGSGRPEAEGLRASARLKDGIAQLSAERIWSEMKKLLGAPDPSRALLWMRQAGVLNLVLP
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEEC
ESEKWGIDAIHGLVRTQSDLGWQADPLLRLESLVPPDAVRMEELGKRMKMSNAERARLEA
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHH
WARADAVKPGLSEQALKKAIYRGSKQALLDRIRLAYAAARQEAVGSDEAMIRAGGVARLL
HHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHH
DAAEHYDAPVFPITGGDLLARGIEKGPGLGETLRSLETLWIDSCFSLDREALLRKLDRDN
HHHHHCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
LND
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA