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The map label for this gene is murD [H]

Identifier: 23502304

GI number: 23502304

Start: 1390550

End: 1391953

Strand: Reverse

Name: murD [H]

Synonym: BR1433

Alternate gene names: 23502304

Gene position: 1391953-1390550 (Counterclockwise)

Preceding gene: 23502305

Following gene: 23502303

Centisome position: 66.04

GC content: 60.47

Gene sequence:

>1404_bases
ATGATCCCGATCACCGCCCTCAAGGATAAAACTGTCGCCCTTTTCGGACTGGGCGGGTCGGGCATTGCGACTGCAAAGGC
CATTGTGGCGGGCGGTGCAAGGATCATTGCCTGGGACGATAATCCCGACAGTGTGGCCCGCGCGCAAAGTGCTGGCATTG
CAACGGGCGATCTGCGGCAGGCGGACTGGTCACAGTTCGCCGTCTTCGTGCTTTCGCCGGGGGTTCCGCTGACCCATCCG
CAGCCGCACTGGTCGGTGGACCTTGCCCGCGCGGCAGGTGTTGAAATTATCGGCGATGTGGAACTTTTCGTGCGTGAACG
CAACCATATCGCACCGGACTGCCCTTTCATCGCCATCACCGGCACCAACGGGAAATCCACGACCACGGCGCTGATCGCCC
ATATCATCAAGGCCACGGGCCGGGATATGCAGCTTGGCGGCAATATCGGCACCGCGATCCTGACACTCGAACCGCCGTGT
GCCGACCGTTTCTACGTGGTCGAATGCTCATCCTACCAGATCGACCTTGCGCCATCGCTGAACCCGACCGCGGGCATTCT
CCTGAACCTGACGCCCGATCATCTCGACCGTCACGGTTCGATGGAAAACTATGCCGCTATCAAGGAACGGCTCGTCGCGG
CAAGCGGTACGGCGATCATCGGCATCGATGATGCTTATTGCCAGGCCATTGCCGACCGGCTGCATGGCGCTGGAATACGG
GTGGTGCGCATTTCCAAGGAAAAGCATCTCGACAGAGGCTATTTTGCCGATGGCGCAAAGCTGCTTTGGGCGCAGGATGG
GGAAATTGATGAAATCGCCTCGCTGGAAGGCATCGGTTCGCTGCGCGGCGCGCATAATGCGCAGAACGCGCTGGCGGCGA
TCGTCGCCTGCCTGTCGGCAGGCTTGAGCCTTGAAGAAATCCATGCCGGGTTGAAGAGTTTCCCCGGTCTGGCCCATCGC
ATGGAACAGGTCGGGCGTCGCGGGAAAGTCCTGTTTGTCAACGATTCCAAGGCTACCAATGCGGAAGCGACGGCGCCTGC
GCTCTCTTCCTTTCCGCAGAATATCTACTGGATTGTGGGCGGAGTACCGAAAGCCGGTGGCATCAATTCGCTCACCGCGT
TTTTCCCAAGGGTCGCCAAGGCCTATCTGATCGGTGAAGCTGCCGCCCAGTTCGCGGCCACACTCGGCGGCGCCGTGCCG
TTCGAGATTTCCGATACGCTGGCAGCCGCAGTGGCACATGCGGCAGGTGATGCGGGCAATGATGCTGCGCCCGAACCTGT
TGTGCTGCTGTCACCCGCCTGCGCAAGCTTCGACCAGTTCCAGAATTTCGAGAAACGCGGCGACGCTTTCCGCGATGCCG
TTCTCGCTCTTCCGGGTGTCATGCCAATGAGAGGAGAAAGCTGA

Upstream 100 bases:

>100_bases
CTGGACCGAGAGCCAGGTCGTGATCCGGTTCTGGATCGTCGCGATCATCCTCGCAATGATCGGCCTTTCCACATTGAAAC
TCAGATAACAGGCGAACAAA

Downstream 100 bases:

>100_bases
TGGTAAGCCGTGTCGATCGCGGTCCCGTTGCCAATTGGTGGTGGACGATAGACCGCTTTTTCCTTGCCGCGTGTCTGGCG
CTGATGGGACTTGGAATTCT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MIPITALKDKTVALFGLGGSGIATAKAIVAGGARIIAWDDNPDSVARAQSAGIATGDLRQADWSQFAVFVLSPGVPLTHP
QPHWSVDLARAAGVEIIGDVELFVRERNHIAPDCPFIAITGTNGKSTTTALIAHIIKATGRDMQLGGNIGTAILTLEPPC
ADRFYVVECSSYQIDLAPSLNPTAGILLNLTPDHLDRHGSMENYAAIKERLVAASGTAIIGIDDAYCQAIADRLHGAGIR
VVRISKEKHLDRGYFADGAKLLWAQDGEIDEIASLEGIGSLRGAHNAQNALAAIVACLSAGLSLEEIHAGLKSFPGLAHR
MEQVGRRGKVLFVNDSKATNAEATAPALSSFPQNIYWIVGGVPKAGGINSLTAFFPRVAKAYLIGEAAAQFAATLGGAVP
FEISDTLAAAVAHAAGDAGNDAAPEPVVLLSPACASFDQFQNFEKRGDAFRDAVLALPGVMPMRGES

Sequences:

>Translated_467_residues
MIPITALKDKTVALFGLGGSGIATAKAIVAGGARIIAWDDNPDSVARAQSAGIATGDLRQADWSQFAVFVLSPGVPLTHP
QPHWSVDLARAAGVEIIGDVELFVRERNHIAPDCPFIAITGTNGKSTTTALIAHIIKATGRDMQLGGNIGTAILTLEPPC
ADRFYVVECSSYQIDLAPSLNPTAGILLNLTPDHLDRHGSMENYAAIKERLVAASGTAIIGIDDAYCQAIADRLHGAGIR
VVRISKEKHLDRGYFADGAKLLWAQDGEIDEIASLEGIGSLRGAHNAQNALAAIVACLSAGLSLEEIHAGLKSFPGLAHR
MEQVGRRGKVLFVNDSKATNAEATAPALSSFPQNIYWIVGGVPKAGGINSLTAFFPRVAKAYLIGEAAAQFAATLGGAVP
FEISDTLAAAVAHAAGDAGNDAAPEPVVLLSPACASFDQFQNFEKRGDAFRDAVLALPGVMPMRGES
>Mature_467_residues
MIPITALKDKTVALFGLGGSGIATAKAIVAGGARIIAWDDNPDSVARAQSAGIATGDLRQADWSQFAVFVLSPGVPLTHP
QPHWSVDLARAAGVEIIGDVELFVRERNHIAPDCPFIAITGTNGKSTTTALIAHIIKATGRDMQLGGNIGTAILTLEPPC
ADRFYVVECSSYQIDLAPSLNPTAGILLNLTPDHLDRHGSMENYAAIKERLVAASGTAIIGIDDAYCQAIADRLHGAGIR
VVRISKEKHLDRGYFADGAKLLWAQDGEIDEIASLEGIGSLRGAHNAQNALAAIVACLSAGLSLEEIHAGLKSFPGLAHR
MEQVGRRGKVLFVNDSKATNAEATAPALSSFPQNIYWIVGGVPKAGGINSLTAFFPRVAKAYLIGEAAAQFAATLGGAVP
FEISDTLAAAVAHAAGDAGNDAAPEPVVLLSPACASFDQFQNFEKRGDAFRDAVLALPGVMPMRGES

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=459, Percent_Identity=34.2047930283224, Blast_Score=187, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48766; Mature: 48766

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPITALKDKTVALFGLGGSGIATAKAIVAGGARIIAWDDNPDSVARAQSAGIATGDLRQ
CCCCCEECCCEEEEEECCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCCCCC
ADWSQFAVFVLSPGVPLTHPQPHWSVDLARAAGVEIIGDVELFVRERNHIAPDCPFIAIT
CCCCCEEEEEECCCCCCCCCCCCCCEEHHHHCCCEEEECHHEEEECCCCCCCCCCEEEEE
GTNGKSTTTALIAHIIKATGRDMQLGGNIGTAILTLEPPCADRFYVVECSSYQIDLAPSL
CCCCCHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCEEEEEECCEEEEECCCC
NPTAGILLNLTPDHLDRHGSMENYAAIKERLVAASGTAIIGIDDAYCQAIADRLHGAGIR
CCCCCEEEECCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCEE
VVRISKEKHLDRGYFADGAKLLWAQDGEIDEIASLEGIGSLRGAHNAQNALAAIVACLSA
EEEECHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHC
GLSLEEIHAGLKSFPGLAHRMEQVGRRGKVLFVNDSKATNAEATAPALSSFPQNIYWIVG
CCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHCCCCEEEEEE
GVPKAGGINSLTAFFPRVAKAYLIGEAAAQFAATLGGAVPFEISDTLAAAVAHAAGDAGN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCC
DAAPEPVVLLSPACASFDQFQNFEKRGDAFRDAVLALPGVMPMRGES
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIPITALKDKTVALFGLGGSGIATAKAIVAGGARIIAWDDNPDSVARAQSAGIATGDLRQ
CCCCCEECCCEEEEEECCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCCCCC
ADWSQFAVFVLSPGVPLTHPQPHWSVDLARAAGVEIIGDVELFVRERNHIAPDCPFIAIT
CCCCCEEEEEECCCCCCCCCCCCCCEEHHHHCCCEEEECHHEEEECCCCCCCCCCEEEEE
GTNGKSTTTALIAHIIKATGRDMQLGGNIGTAILTLEPPCADRFYVVECSSYQIDLAPSL
CCCCCHHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCCEEEEEECCEEEEECCCC
NPTAGILLNLTPDHLDRHGSMENYAAIKERLVAASGTAIIGIDDAYCQAIADRLHGAGIR
CCCCCEEEECCHHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCEE
VVRISKEKHLDRGYFADGAKLLWAQDGEIDEIASLEGIGSLRGAHNAQNALAAIVACLSA
EEEECHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHC
GLSLEEIHAGLKSFPGLAHRMEQVGRRGKVLFVNDSKATNAEATAPALSSFPQNIYWIVG
CCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHCCCCEEEEEE
GVPKAGGINSLTAFFPRVAKAYLIGEAAAQFAATLGGAVPFEISDTLAAAVAHAAGDAGN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCC
DAAPEPVVLLSPACASFDQFQNFEKRGDAFRDAVLALPGVMPMRGES
CCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA