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The map label for this gene is miaA [H]

Identifier: 23502261

GI number: 23502261

Start: 1345093

End: 1346025

Strand: Reverse

Name: miaA [H]

Synonym: BR1390

Alternate gene names: 23502261

Gene position: 1346025-1345093 (Counterclockwise)

Preceding gene: 23502263

Following gene: 23502260

Centisome position: 63.86

GC content: 59.49

Gene sequence:

>933_bases
ATGAGTGAGGATGCAGTCAAGAATGCGATCCTGATAGCTGGCCCAACGGCCAGCGGCAAGTCGGCACTTGCCATTCGCAT
GGCCAAAGCGACGGGCGGTTTCATCGTCAATACCGATTCCATGCAGGTTTATGGCGTTCTGGATCTTTTGACCGCGCGGC
CTTCCAGGGCCGATCTGGCCGAGGCCGAACATTTTCTTTATGGCCATGTGCCGCCCTCCAGCACCTATTCCACCGGCAAA
TGGTTTGAGGATGTCGAAGCACTTCTGGGGCGTTGCGGACTGCAAGGCCGGGTGCCGATCTTCGTCGGCGGGACAGGGCT
TTATTTTCGTGCGCTTCTGGGCGGGCTTTCGCAAATGCCGGAAGTATCGGCGCAAGTGCGCGACCATTGGCGAGGCCGGA
TGGAAGCCGAAGGCGCAAAGGCCCTGCACGCTGTCCTTTGTGTGCGTGACCCGGCCATCGCGGCTGCCTTGCAGCCCTCC
GATAGCCAGCGCATTGTGCGTGCGCTGGAAGTGCTGGAAAGCACCGGCAAATCCCTTTTGGAATGGCAAAAGGTCAAGGG
CAGGGCGCTGGTGGATGACCAATCGGCGCAAAAGATCGTGCTCCGGCCTGATCGTGCATGGCTTGGCGAGCGTATTGCGC
GGCGTTTTTCGGCCATGTGGGCGGAGGGGGCAATCGATGAGGTGAGGGCGCTTCTGGCGCTCGATCTCGATCCTGCGCTA
CCCGCCATGAAGGCCATTGGCGTCAGGGAAGTCAGTGCATTTCTTGCAGAAACCATGTCGCGCGAGGAGGCTATTGAACG
TTCCGTCATTGCCACGCGGCAATATGCCAAGCGCCAGTCCACCTGGTTTCGCAATCAGTTGGGCGAAGACTGGCGTGTCT
ATGCCAGTGGCGAAGAGGTGTTTCAGGGCGGTTCGTTTCGGGATCCGCAATAA

Upstream 100 bases:

>100_bases
GCTTTAATGCCGAGCGAAGGGGCAAGCGCGGCTTTTGCCGGGTTGGCGATCAGGGTAGCGACGAGAGAAACCTGCTGAGA
CATGGAGAGCAATTCCGACG

Downstream 100 bases:

>100_bases
AGTTATCGGGTAAGGTGAGTGCGGATAAGAAAATATCGCGGGCCGTGCATTTATGCGTTGACGACACCTTTGCTCGCTAT
TAGTGTCCGCAAGCATGAAC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSEDAVKNAILIAGPTASGKSALAIRMAKATGGFIVNTDSMQVYGVLDLLTARPSRADLAEAEHFLYGHVPPSSTYSTGK
WFEDVEALLGRCGLQGRVPIFVGGTGLYFRALLGGLSQMPEVSAQVRDHWRGRMEAEGAKALHAVLCVRDPAIAAALQPS
DSQRIVRALEVLESTGKSLLEWQKVKGRALVDDQSAQKIVLRPDRAWLGERIARRFSAMWAEGAIDEVRALLALDLDPAL
PAMKAIGVREVSAFLAETMSREEAIERSVIATRQYAKRQSTWFRNQLGEDWRVYASGEEVFQGGSFRDPQ

Sequences:

>Translated_310_residues
MSEDAVKNAILIAGPTASGKSALAIRMAKATGGFIVNTDSMQVYGVLDLLTARPSRADLAEAEHFLYGHVPPSSTYSTGK
WFEDVEALLGRCGLQGRVPIFVGGTGLYFRALLGGLSQMPEVSAQVRDHWRGRMEAEGAKALHAVLCVRDPAIAAALQPS
DSQRIVRALEVLESTGKSLLEWQKVKGRALVDDQSAQKIVLRPDRAWLGERIARRFSAMWAEGAIDEVRALLALDLDPAL
PAMKAIGVREVSAFLAETMSREEAIERSVIATRQYAKRQSTWFRNQLGEDWRVYASGEEVFQGGSFRDPQ
>Mature_309_residues
SEDAVKNAILIAGPTASGKSALAIRMAKATGGFIVNTDSMQVYGVLDLLTARPSRADLAEAEHFLYGHVPPSSTYSTGKW
FEDVEALLGRCGLQGRVPIFVGGTGLYFRALLGGLSQMPEVSAQVRDHWRGRMEAEGAKALHAVLCVRDPAIAAALQPSD
SQRIVRALEVLESTGKSLLEWQKVKGRALVDDQSAQKIVLRPDRAWLGERIARRFSAMWAEGAIDEVRALLALDLDPALP
AMKAIGVREVSAFLAETMSREEAIERSVIATRQYAKRQSTWFRNQLGEDWRVYASGEEVFQGGSFRDPQ

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=308, Percent_Identity=27.9220779220779, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1790613, Length=292, Percent_Identity=39.7260273972603, Blast_Score=187, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI25144712, Length=301, Percent_Identity=23.9202657807309, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324848, Length=305, Percent_Identity=27.8688524590164, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24649830, Length=330, Percent_Identity=25.4545454545455, Blast_Score=98, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 33943; Mature: 33812

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDAVKNAILIAGPTASGKSALAIRMAKATGGFIVNTDSMQVYGVLDLLTARPSRADLA
CCCHHHHCEEEEECCCCCCCCEEEEEEEECCCCEEEECCCEEHHHHHHHHHCCCCCHHHH
EAEHFLYGHVPPSSTYSTGKWFEDVEALLGRCGLQGRVPIFVGGTGLYFRALLGGLSQMP
HHHHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHCH
EVSAQVRDHWRGRMEAEGAKALHAVLCVRDPAIAAALQPSDSQRIVRALEVLESTGKSLL
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHEEECCCHHHHHHHHHHHHHHHHHHHH
EWQKVKGRALVDDQSAQKIVLRPDRAWLGERIARRFSAMWAEGAIDEVRALLALDLDPAL
HHHHHCCCEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCH
PAMKAIGVREVSAFLAETMSREEAIERSVIATRQYAKRQSTWFRNQLGEDWRVYASGEEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHH
FQGGSFRDPQ
HCCCCCCCCC
>Mature Secondary Structure 
SEDAVKNAILIAGPTASGKSALAIRMAKATGGFIVNTDSMQVYGVLDLLTARPSRADLA
CCHHHHCEEEEECCCCCCCCEEEEEEEECCCCEEEECCCEEHHHHHHHHHCCCCCHHHH
EAEHFLYGHVPPSSTYSTGKWFEDVEALLGRCGLQGRVPIFVGGTGLYFRALLGGLSQMP
HHHHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHCH
EVSAQVRDHWRGRMEAEGAKALHAVLCVRDPAIAAALQPSDSQRIVRALEVLESTGKSLL
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHEEECCCHHHHHHHHHHHHHHHHHHHH
EWQKVKGRALVDDQSAQKIVLRPDRAWLGERIARRFSAMWAEGAIDEVRALLALDLDPAL
HHHHHCCCEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCH
PAMKAIGVREVSAFLAETMSREEAIERSVIATRQYAKRQSTWFRNQLGEDWRVYASGEEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHH
FQGGSFRDPQ
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA