Click here to switch to the map view.

The map label for this gene is ilvB [H]

Identifier: 23502260

GI number: 23502260

Start: 1343022

End: 1344776

Strand: Reverse

Name: ilvB [H]

Synonym: BR1389

Alternate gene names: 23502260

Gene position: 1344776-1343022 (Counterclockwise)

Preceding gene: 23502261

Following gene: 23502259

Centisome position: 63.8

GC content: 58.4

Gene sequence:

>1755_bases
ATGACCGGCGCAGAAATGGTTATCCAGGCGCTGATCGATCAGGGTGTGGAAAGCATTTTCGGCTATCCCGGCGGCGCGGT
GCTTCCGATTTATGACGAGCTGTTTCAGCAGGATCAAATCCAGCACGTTCTGGTTCGCCATGAGCAGGGTGCAGGCCATG
CAGCGGAAGGCTATGCGCGCTCGACGGGTAAAGTCGGCGTCATGCTCGTCACTTCGGGGCCGGGTGCAACCAATGCCGTG
ACGCCGTTGCAGGATGCCTTGATGGATTCCATCCCGCTGGTCTGCATTTCCGGTCAGGTTCCCACGACGCTGATCGGTTC
CGATGCGTTTCAGGAATGCGATACGATTGGCATCACGCGCCCCTGCACCAAGCACAACTGGCTGGTAAAGGACGTCAACG
ACCTGTCGCGCGTCCTGCATGAAGCTTTCCATGTCGCATCCACCGGCCGTCCCGGCCCCGTCGTCGTCGATATTCCGAAG
GATATTCAGTTCGCCAAGGGCATCTATACGCCGCCGGAAACTGCACCGCGCACAAGTTACCGTCCAGTTCTCGACGGCAA
TGCCGAGGCTATTTCCGAGGCTGTCCGTCTGCTCGCCAATGCCAGAAAGCCGGTCATTTATTCGGGCGGCGGCGTGATCA
ATTCCGGCCCCGCGGCTTCCCGTCTGCTGCGTGAGCTGGTCGAGATCAGCAATTTCCCGATCACCTCCACGCTGATGGGG
CTTGGCGCCTATCCCGCCTCCGGCAAGAACTGGCTCGGTATGCTGGGCATGCACGGCACCTATGAAGCCAACATGACCAT
GCATGATTGCGATGTCATGCTGTGCGTCGGTGCGCGTTTCGACGACCGCATCACCGGCCGCCTCAACGCTTTCTCGCCGA
ATTCCAAGAAAATTCATATCGATATCGATCCGTCGTCGATCAACAAGAACGTTCGGGTCGATGTGCCGATCATCGGCGAT
GTCGCGCATGTGCTGGAGGATATCGTCCGCCAGTTTCGTGCTTTCGACCGCAAGCCAGACAGGCAGGCATTGACCGAATG
GTGGGGGCAGATCGACCGTTGGCGCGCGCGCAATTCGCTGGCCTATACGCCGAACAAAGACGTCATCATGCCGCAATATG
CCATCGAGCGGCTTTATGAACTGACCAGGAACCGCAACACCTATATCACCACCGAGGTGGGCCAGCACCAGATGTGGGCG
GCGCAATTCTTCGGCTTTGAACAGCCGAACCGTTGGCTGACTTCCGGCGGCCTTGGCACCATGGGCTATGGTCTGCCTGC
GGCGCTGGGCGTGCAGATTGCACATCCGGATGCGCTGGTTATCGATATTGCAGGCGATGCCTCGATCCAGATGTGTATCC
AGGAAATGTCGGCAGCAATCCAGCATAATGCGCCGATCAAGATTTTCATCCTGAACAACCAGTATATGGGCATGGTGCGC
CAGTGGCAGCAATTGCTGCATGGCAACCGCCTGTCGCATTCCTACACGGAAGCGATGCCCGATTTCGTGAAACTTGCGGA
AGCATACGGCGCGCACGGCATTCGCTGCGAGAAGCCGGGCGATCTCGATGCCGCCATTCAGGAGATGATCGATATCGACA
AGCCGGTCATTTTCGATTGCCGCGTGGCAAACCTTGCCAACTGCTTCCCGATGATCCCGTCGGGCAAGGCGCATAATGAA
ATGCTGTTGCCGGACGAAGCGACAGATGAGGCCGTCGCCAATGCCATTGATGCCAAGGGCCGCGCGCTCGTCTGA

Upstream 100 bases:

>100_bases
GAAATGAAATGCAGACGACGGGAAGAGAACGATGAATGCAGGTAAACATGAGGCGGAAGCAAACGCCGGGCAAGCGCAGG
ACGCAGGCCATCCACGCGAA

Downstream 100 bases:

>100_bases
TCCGAAGGAATTGAAATCATGAACGCACAAAACCTAGCTTCCGGATCGGCCTATTTCATTGCTGCCGAAACCCAGACGCC
CGAAACCCACACGCTTGCAG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MTGAEMVIQALIDQGVESIFGYPGGAVLPIYDELFQQDQIQHVLVRHEQGAGHAAEGYARSTGKVGVMLVTSGPGATNAV
TPLQDALMDSIPLVCISGQVPTTLIGSDAFQECDTIGITRPCTKHNWLVKDVNDLSRVLHEAFHVASTGRPGPVVVDIPK
DIQFAKGIYTPPETAPRTSYRPVLDGNAEAISEAVRLLANARKPVIYSGGGVINSGPAASRLLRELVEISNFPITSTLMG
LGAYPASGKNWLGMLGMHGTYEANMTMHDCDVMLCVGARFDDRITGRLNAFSPNSKKIHIDIDPSSINKNVRVDVPIIGD
VAHVLEDIVRQFRAFDRKPDRQALTEWWGQIDRWRARNSLAYTPNKDVIMPQYAIERLYELTRNRNTYITTEVGQHQMWA
AQFFGFEQPNRWLTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASIQMCIQEMSAAIQHNAPIKIFILNNQYMGMVR
QWQQLLHGNRLSHSYTEAMPDFVKLAEAYGAHGIRCEKPGDLDAAIQEMIDIDKPVIFDCRVANLANCFPMIPSGKAHNE
MLLPDEATDEAVANAIDAKGRALV

Sequences:

>Translated_584_residues
MTGAEMVIQALIDQGVESIFGYPGGAVLPIYDELFQQDQIQHVLVRHEQGAGHAAEGYARSTGKVGVMLVTSGPGATNAV
TPLQDALMDSIPLVCISGQVPTTLIGSDAFQECDTIGITRPCTKHNWLVKDVNDLSRVLHEAFHVASTGRPGPVVVDIPK
DIQFAKGIYTPPETAPRTSYRPVLDGNAEAISEAVRLLANARKPVIYSGGGVINSGPAASRLLRELVEISNFPITSTLMG
LGAYPASGKNWLGMLGMHGTYEANMTMHDCDVMLCVGARFDDRITGRLNAFSPNSKKIHIDIDPSSINKNVRVDVPIIGD
VAHVLEDIVRQFRAFDRKPDRQALTEWWGQIDRWRARNSLAYTPNKDVIMPQYAIERLYELTRNRNTYITTEVGQHQMWA
AQFFGFEQPNRWLTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASIQMCIQEMSAAIQHNAPIKIFILNNQYMGMVR
QWQQLLHGNRLSHSYTEAMPDFVKLAEAYGAHGIRCEKPGDLDAAIQEMIDIDKPVIFDCRVANLANCFPMIPSGKAHNE
MLLPDEATDEAVANAIDAKGRALV
>Mature_583_residues
TGAEMVIQALIDQGVESIFGYPGGAVLPIYDELFQQDQIQHVLVRHEQGAGHAAEGYARSTGKVGVMLVTSGPGATNAVT
PLQDALMDSIPLVCISGQVPTTLIGSDAFQECDTIGITRPCTKHNWLVKDVNDLSRVLHEAFHVASTGRPGPVVVDIPKD
IQFAKGIYTPPETAPRTSYRPVLDGNAEAISEAVRLLANARKPVIYSGGGVINSGPAASRLLRELVEISNFPITSTLMGL
GAYPASGKNWLGMLGMHGTYEANMTMHDCDVMLCVGARFDDRITGRLNAFSPNSKKIHIDIDPSSINKNVRVDVPIIGDV
AHVLEDIVRQFRAFDRKPDRQALTEWWGQIDRWRARNSLAYTPNKDVIMPQYAIERLYELTRNRNTYITTEVGQHQMWAA
QFFGFEQPNRWLTSGGLGTMGYGLPAALGVQIAHPDALVIDIAGDASIQMCIQEMSAAIQHNAPIKIFILNNQYMGMVRQ
WQQLLHGNRLSHSYTEAMPDFVKLAEAYGAHGIRCEKPGDLDAAIQEMIDIDKPVIFDCRVANLANCFPMIPSGKAHNEM
LLPDEATDEAVANAIDAKGRALV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=570, Percent_Identity=24.9122807017544, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI21361361, Length=549, Percent_Identity=25.3187613843352, Blast_Score=139, Evalue=1e-32,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=51.4084507042254, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1790104, Length=563, Percent_Identity=41.563055062167, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1786717, Length=568, Percent_Identity=34.330985915493, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=28.6751361161525, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1788716, Length=563, Percent_Identity=24.5115452930728, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=570, Percent_Identity=25.9649122807018, Blast_Score=152, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=570, Percent_Identity=25.9649122807018, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=522, Percent_Identity=26.2452107279693, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=585, Percent_Identity=43.4188034188034, Blast_Score=499, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6320816, Length=492, Percent_Identity=24.5934959349593, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI19922626, Length=563, Percent_Identity=25.044404973357, Blast_Score=158, Evalue=1e-38,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63907; Mature: 63776

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGAEMVIQALIDQGVESIFGYPGGAVLPIYDELFQQDQIQHVLVRHEQGAGHAAEGYAR
CCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
STGKVGVMLVTSGPGATNAVTPLQDALMDSIPLVCISGQVPTTLIGSDAFQECDTIGITR
CCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCEEEECCCCCCEEECCHHHHHHHHCCCCC
PCTKHNWLVKDVNDLSRVLHEAFHVASTGRPGPVVVDIPKDIQFAKGIYTPPETAPRTSY
CCCCCCCHHHCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHCCCCCCCCCCCCCCC
RPVLDGNAEAISEAVRLLANARKPVIYSGGGVINSGPAASRLLRELVEISNFPITSTLMG
CCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LGAYPASGKNWLGMLGMHGTYEANMTMHDCDVMLCVGARFDDRITGRLNAFSPNSKKIHI
CCCCCCCCCCCEEEECCCCEEECCCEEECCCEEEEECCCCCCCCCCEEECCCCCCCEEEE
DIDPSSINKNVRVDVPIIGDVAHVLEDIVRQFRAFDRKPDRQALTEWWGQIDRWRARNSL
EECHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
AYTPNKDVIMPQYAIERLYELTRNRNTYITTEVGQHQMWAAQFFGFEQPNRWLTSGGLGT
EECCCCCEECHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCEEECCCCCC
MGYGLPAALGVQIAHPDALVIDIAGDASIQMCIQEMSAAIQHNAPIKIFILNNQYMGMVR
CCCCCHHHHCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHH
QWQQLLHGNRLSHSYTEAMPDFVKLAEAYGAHGIRCEKPGDLDAAIQEMIDIDKPVIFDC
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCEEEEE
RVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRALV
EHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TGAEMVIQALIDQGVESIFGYPGGAVLPIYDELFQQDQIQHVLVRHEQGAGHAAEGYAR
CHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
STGKVGVMLVTSGPGATNAVTPLQDALMDSIPLVCISGQVPTTLIGSDAFQECDTIGITR
CCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCEEEECCCCCCEEECCHHHHHHHHCCCCC
PCTKHNWLVKDVNDLSRVLHEAFHVASTGRPGPVVVDIPKDIQFAKGIYTPPETAPRTSY
CCCCCCCHHHCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHCCCCCCCCCCCCCCC
RPVLDGNAEAISEAVRLLANARKPVIYSGGGVINSGPAASRLLRELVEISNFPITSTLMG
CCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LGAYPASGKNWLGMLGMHGTYEANMTMHDCDVMLCVGARFDDRITGRLNAFSPNSKKIHI
CCCCCCCCCCCEEEECCCCEEECCCEEECCCEEEEECCCCCCCCCCEEECCCCCCCEEEE
DIDPSSINKNVRVDVPIIGDVAHVLEDIVRQFRAFDRKPDRQALTEWWGQIDRWRARNSL
EECHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
AYTPNKDVIMPQYAIERLYELTRNRNTYITTEVGQHQMWAAQFFGFEQPNRWLTSGGLGT
EECCCCCEECHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCEEECCCCCC
MGYGLPAALGVQIAHPDALVIDIAGDASIQMCIQEMSAAIQHNAPIKIFILNNQYMGMVR
CCCCCHHHHCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHH
QWQQLLHGNRLSHSYTEAMPDFVKLAEAYGAHGIRCEKPGDLDAAIQEMIDIDKPVIFDC
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCEEEEE
RVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRALV
EHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]