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The map label for this gene is cobB [H]

Identifier: 23502172

GI number: 23502172

Start: 1256538

End: 1257848

Strand: Reverse

Name: cobB [H]

Synonym: BR1296

Alternate gene names: 23502172

Gene position: 1257848-1256538 (Counterclockwise)

Preceding gene: 23502173

Following gene: 23502171

Centisome position: 59.68

GC content: 60.49

Gene sequence:

>1311_bases
ATGAAGGGATTCATGATTGCCGCACCGGCCTCCGGGTCGGGCAAGACGACCGTGACCTTGGGTTTGTTGCGCGCCCTGAA
ACGCAGGGGCGAGGTGCTTGCGCCGGTGAAGGCCGGGCCGGATTATATCGACCCCGCCTATCACAGGGCGGCGAGTGGCG
TGGATTGTTTCAATCTCGACCCCTGGGCCATGCGACCGGGACTCATCAGTGCGCTTTCCTCCCGCATGACGGAAAGCGGC
GCGCGTGTTCTTGTCGCCGAAGGCATGATGGGGCTTTTCGACGGGGCGATTGACGGTAAGGGCTCTTCTGCCGATCTGGC
GCGGCTTCTGGATTTGCCAGTCGTGCTGGTGGTGGATTGCGCCCGGCAATCCCACTCCATCGCGGCGCTGGTGTGGGGTT
TCAGCCAGTTTCGCAAGGATGTGCTGATTGAGGGTGTCATTCTCAATCGTGTCGGAAGTCCCCGCCATGAAGCCATGCTG
CGGGGCGCTCTGGCACCCCTTGGCGTCCCGGTTCTGGGCGCGCTGCCGCGAGATCCGGCACTTTCCTTGCCGGAAAGGCA
TCTGGGGCTGGTGCAGGCGGACGAACATGCCGGTCTTGAAAGCTTTCTCGAACAAGCGGCCGATGTGATGGAAGCGCATA
TCGACATGGATGCGCTTCAGACCATCTGGCTACGCCCGAAGCGCTATGACGCCATGGCCAATGTCGCGCGTCTGAAACCG
CTTGGAAACCGCATTGCCGTGGCGCGGGACGATGCCTTTGCTTTTGCCTATATGCATCTTTTTGAAGGCTGGCGCAGGCG
GGGTGCGGAAATCTCTTTCTTTTCCCCGCTGGCAGATGAAGCACCGAAGGCAGATGCGGATGCAATCTATCTTCCCGGTG
GTTATCCAGAACTTCATGCGCAACGATTGGCCGGTGCATCCCGTTTCCGCACGGCGATAGGGGACGCTGCCGCGCGTGGC
GTCACGGTCTATGGCGAGTGCGGCGGCTATATGGTCCTCGGGAAAACACTGGAGGATGCGGCAGGCGTTCACCATCCCAT
GCTGGGGCTTTTGCCATTGGAAACGAGCTTTGCCAGGCGGAAACTGCATCTTGGCTATCGCCTGCTGGAGCCGCTTGGCG
GATTGCCGTGGGACATGCCTTTGAAAGCACATGAATTCCATTATGCAAGCATCGTGCGGGAAGAAAAGGCCGACCGCCTG
TTCCGGGTGCGCGATGCATCGGGCGAGAACCTTGGCGAAGCGGGCTTGCGGGTTGGTTCCGTCTCCGGCTCCTTCATGCA
TGTCATTGATTTTAGCGGAGAAGCGGCGTGA

Upstream 100 bases:

>100_bases
TGCGCTTGCGTGCCGGGCTGGACTGGATCGGCGCAATGGCGGGAAAAAGCCTTGTCAGCGATCCGCTGGGCAAGAGAACG
CAACAGGGAGTGCAGAGCGC

Downstream 100 bases:

>100_bases
GCGTGGAAATCGAGCATGGTGGCGCGCTGGACCGGGCCATCGCGCGCTTTGGCGGCTCGGCGCAGGACTGGCTCGATCTT
TCAACGGGCATCAATCCCGA

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MKGFMIAAPASGSGKTTVTLGLLRALKRRGEVLAPVKAGPDYIDPAYHRAASGVDCFNLDPWAMRPGLISALSSRMTESG
ARVLVAEGMMGLFDGAIDGKGSSADLARLLDLPVVLVVDCARQSHSIAALVWGFSQFRKDVLIEGVILNRVGSPRHEAML
RGALAPLGVPVLGALPRDPALSLPERHLGLVQADEHAGLESFLEQAADVMEAHIDMDALQTIWLRPKRYDAMANVARLKP
LGNRIAVARDDAFAFAYMHLFEGWRRRGAEISFFSPLADEAPKADADAIYLPGGYPELHAQRLAGASRFRTAIGDAAARG
VTVYGECGGYMVLGKTLEDAAGVHHPMLGLLPLETSFARRKLHLGYRLLEPLGGLPWDMPLKAHEFHYASIVREEKADRL
FRVRDASGENLGEAGLRVGSVSGSFMHVIDFSGEAA

Sequences:

>Translated_436_residues
MKGFMIAAPASGSGKTTVTLGLLRALKRRGEVLAPVKAGPDYIDPAYHRAASGVDCFNLDPWAMRPGLISALSSRMTESG
ARVLVAEGMMGLFDGAIDGKGSSADLARLLDLPVVLVVDCARQSHSIAALVWGFSQFRKDVLIEGVILNRVGSPRHEAML
RGALAPLGVPVLGALPRDPALSLPERHLGLVQADEHAGLESFLEQAADVMEAHIDMDALQTIWLRPKRYDAMANVARLKP
LGNRIAVARDDAFAFAYMHLFEGWRRRGAEISFFSPLADEAPKADADAIYLPGGYPELHAQRLAGASRFRTAIGDAAARG
VTVYGECGGYMVLGKTLEDAAGVHHPMLGLLPLETSFARRKLHLGYRLLEPLGGLPWDMPLKAHEFHYASIVREEKADRL
FRVRDASGENLGEAGLRVGSVSGSFMHVIDFSGEAA
>Mature_436_residues
MKGFMIAAPASGSGKTTVTLGLLRALKRRGEVLAPVKAGPDYIDPAYHRAASGVDCFNLDPWAMRPGLISALSSRMTESG
ARVLVAEGMMGLFDGAIDGKGSSADLARLLDLPVVLVVDCARQSHSIAALVWGFSQFRKDVLIEGVILNRVGSPRHEAML
RGALAPLGVPVLGALPRDPALSLPERHLGLVQADEHAGLESFLEQAADVMEAHIDMDALQTIWLRPKRYDAMANVARLKP
LGNRIAVARDDAFAFAYMHLFEGWRRRGAEISFFSPLADEAPKADADAIYLPGGYPELHAQRLAGASRFRTAIGDAAARG
VTVYGECGGYMVLGKTLEDAAGVHHPMLGLLPLETSFARRKLHLGYRLLEPLGGLPWDMPLKAHEFHYASIVREEKADRL
FRVRDASGENLGEAGLRVGSVSGSFMHVIDFSGEAA

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 46963; Mature: 46963

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGFMIAAPASGSGKTTVTLGLLRALKRRGEVLAPVKAGPDYIDPAYHRAASGVDCFNLD
CCCCEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHCCCCCCCCCC
PWAMRPGLISALSSRMTESGARVLVAEGMMGLFDGAIDGKGSSADLARLLDLPVVLVVDC
CHHCCHHHHHHHHHHHHHCCCEEEEECCHHHHHCCCCCCCCCHHHHHHHHCCCHHEEEHH
ARQSHSIAALVWGFSQFRKDVLIEGVILNRVGSPRHEAMLRGALAPLGVPVLGALPRDPA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCC
LSLPERHLGLVQADEHAGLESFLEQAADVMEAHIDMDALQTIWLRPKRYDAMANVARLKP
CCCCHHHCCCEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHCC
LGNRIAVARDDAFAFAYMHLFEGWRRRGAEISFFSPLADEAPKADADAIYLPGGYPELHA
CCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCCEEEECCCCHHHHH
QRLAGASRFRTAIGDAAARGVTVYGECGGYMVLGKTLEDAAGVHHPMLGLLPLETSFARR
HHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCHHHHCCCCCCCCEEEECCHHHHHH
KLHLGYRLLEPLGGLPWDMPLKAHEFHYASIVREEKADRLFRVRDASGENLGEAGLRVGS
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHCCEEEC
VSGSFMHVIDFSGEAA
CCCCEEEEEECCCCCC
>Mature Secondary Structure
MKGFMIAAPASGSGKTTVTLGLLRALKRRGEVLAPVKAGPDYIDPAYHRAASGVDCFNLD
CCCCEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHCCCCCCCCCC
PWAMRPGLISALSSRMTESGARVLVAEGMMGLFDGAIDGKGSSADLARLLDLPVVLVVDC
CHHCCHHHHHHHHHHHHHCCCEEEEECCHHHHHCCCCCCCCCHHHHHHHHCCCHHEEEHH
ARQSHSIAALVWGFSQFRKDVLIEGVILNRVGSPRHEAMLRGALAPLGVPVLGALPRDPA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCC
LSLPERHLGLVQADEHAGLESFLEQAADVMEAHIDMDALQTIWLRPKRYDAMANVARLKP
CCCCHHHCCCEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHCC
LGNRIAVARDDAFAFAYMHLFEGWRRRGAEISFFSPLADEAPKADADAIYLPGGYPELHA
CCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCCEEEECCCCHHHHH
QRLAGASRFRTAIGDAAARGVTVYGECGGYMVLGKTLEDAAGVHHPMLGLLPLETSFARR
HHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCHHHHCCCCCCCCEEEECCHHHHHH
KLHLGYRLLEPLGGLPWDMPLKAHEFHYASIVREEKADRLFRVRDASGENLGEAGLRVGS
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHCCEEEC
VSGSFMHVIDFSGEAA
CCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA