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The map label for this gene is cobD [H]

Identifier: 23502170

GI number: 23502170

Start: 1254523

End: 1255518

Strand: Reverse

Name: cobD [H]

Synonym: BR1294

Alternate gene names: 23502170

Gene position: 1255518-1254523 (Counterclockwise)

Preceding gene: 23502171

Following gene: 23502168

Centisome position: 59.57

GC content: 59.04

Gene sequence:

>996_bases
TTGGCCAACTCCATGGAAATAAAACTTATTGTCCTTTCGCTGGCGCTCCTTCTCGACCGCTTCATCGGTGATCCACCGCT
GCTTTGGCAAAGGATTTCGCATCCGGTCGTGCTCTTGGGCAAGGCGATTTCCTGGGGTGAAAAAAATTTCAACGACAGCA
GCCTGCCGCCTTCAACGTTGCGCCGCAATGGCATGTGGCTGACGGTTGGGCTGGTGGCGGCTTGTATTTTCGCCGGATTG
GTGATCCGGAGTATTTTGCCCCATGCCGGTACGGCGGGTGCTATTGTCGAAGTCGTAATTGTTGCCATTCTGCTTGCGCA
AAAAAGCCTTGCCGATCATGTGCAGGCCGTGGCGCAAGCCTTGCGTGACGATGGCATAGAAGGTGGCCGCAAGGCGGTCT
CCATGATCGTGGGGCGAAACCCCGACCGACTGGATGAAGGCGGCGTCAGCCGTGCTGCCATTGAAAGCCTTGCGGAAAAT
GCATCCGACGGGATCGTTGCGCCGGCATTCTGGTTTCTGGTCGGCGGATTGCCGGGGCTGTTTGCCTATAAGCTTATCAA
TACGGCCGATTCCATGATCGGGCATCTGAATGATCGCTATCGCGATTTCGGCCGGTTCGCTGCAAAACTCGATGATGTCG
CCAATTATATTCCTGCCCGGCTGACAGGTTTGCTCGCCGCACTTGCAACCTCCCTCGGCCATGGCAGGGAAGCGGGGAGG
CAGGCGCTCTCGATCATGTGCCGGGATGCTAGGCTGCATCGATCGCCCAATGCGGGCTGGCCGGAAGCGGCATTTGCAGG
GGCGCTGGGGCTGGCGCTTGCCGGTCCGCGCCAATATGGCGCCGAGACGGTTGAGGGGCCGATGCTCAACGCCACTGGAA
AACGCGAGGCCGAGGCGAGGGATATCGATGCCGCGCTGGTGCTGTTCTGGTCCACGATGAGCCTGATGACCGGACTGGTG
ATAGCGGCGAGCCTTGTCGGCCTATTTGTCGGTTAG

Upstream 100 bases:

>100_bases
GGATTGGCTGCGTATCGGCCTTGCGCATGACGACGCTGCCCTGGCGCGGCTGGAACAAGCATTGTGCGACATGTGAAGTT
GAAATTTTGATTCAGGTTTT

Downstream 100 bases:

>100_bases
GATGAACCGTTTGCCGTCGAAGCGCTGCAAACGGTTGGGATTGTCCGTCCTGATGCCGTTTTCATCAAATTTGATGGTGC
CCAGAGCGGTTTCAAAGGAG

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MANSMEIKLIVLSLALLLDRFIGDPPLLWQRISHPVVLLGKAISWGEKNFNDSSLPPSTLRRNGMWLTVGLVAACIFAGL
VIRSILPHAGTAGAIVEVVIVAILLAQKSLADHVQAVAQALRDDGIEGGRKAVSMIVGRNPDRLDEGGVSRAAIESLAEN
ASDGIVAPAFWFLVGGLPGLFAYKLINTADSMIGHLNDRYRDFGRFAAKLDDVANYIPARLTGLLAALATSLGHGREAGR
QALSIMCRDARLHRSPNAGWPEAAFAGALGLALAGPRQYGAETVEGPMLNATGKREAEARDIDAALVLFWSTMSLMTGLV
IAASLVGLFVG

Sequences:

>Translated_331_residues
MANSMEIKLIVLSLALLLDRFIGDPPLLWQRISHPVVLLGKAISWGEKNFNDSSLPPSTLRRNGMWLTVGLVAACIFAGL
VIRSILPHAGTAGAIVEVVIVAILLAQKSLADHVQAVAQALRDDGIEGGRKAVSMIVGRNPDRLDEGGVSRAAIESLAEN
ASDGIVAPAFWFLVGGLPGLFAYKLINTADSMIGHLNDRYRDFGRFAAKLDDVANYIPARLTGLLAALATSLGHGREAGR
QALSIMCRDARLHRSPNAGWPEAAFAGALGLALAGPRQYGAETVEGPMLNATGKREAEARDIDAALVLFWSTMSLMTGLV
IAASLVGLFVG
>Mature_330_residues
ANSMEIKLIVLSLALLLDRFIGDPPLLWQRISHPVVLLGKAISWGEKNFNDSSLPPSTLRRNGMWLTVGLVAACIFAGLV
IRSILPHAGTAGAIVEVVIVAILLAQKSLADHVQAVAQALRDDGIEGGRKAVSMIVGRNPDRLDEGGVSRAAIESLAENA
SDGIVAPAFWFLVGGLPGLFAYKLINTADSMIGHLNDRYRDFGRFAAKLDDVANYIPARLTGLLAALATSLGHGREAGRQ
ALSIMCRDARLHRSPNAGWPEAAFAGALGLALAGPRQYGAETVEGPMLNATGKREAEARDIDAALVLFWSTMSLMTGLVI
AASLVGLFVG

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 35021; Mature: 34890

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANSMEIKLIVLSLALLLDRFIGDPPLLWQRISHPVVLLGKAISWGEKNFNDSSLPPSTL
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEHHHHHHCCCCCCCCCCCCHHHH
RRNGMWLTVGLVAACIFAGLVIRSILPHAGTAGAIVEVVIVAILLAQKSLADHVQAVAQA
HHCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDDGIEGGRKAVSMIVGRNPDRLDEGGVSRAAIESLAENASDGIVAPAFWFLVGGLPGL
HHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHH
FAYKLINTADSMIGHLNDRYRDFGRFAAKLDDVANYIPARLTGLLAALATSLGHGREAGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
QALSIMCRDARLHRSPNAGWPEAAFAGALGLALAGPRQYGAETVEGPMLNATGKREAEAR
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHCCHHHCCCCCCCCCCCCCHHH
DIDAALVLFWSTMSLMTGLVIAASLVGLFVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANSMEIKLIVLSLALLLDRFIGDPPLLWQRISHPVVLLGKAISWGEKNFNDSSLPPSTL
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEHHHHHHCCCCCCCCCCCCHHHH
RRNGMWLTVGLVAACIFAGLVIRSILPHAGTAGAIVEVVIVAILLAQKSLADHVQAVAQA
HHCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRDDGIEGGRKAVSMIVGRNPDRLDEGGVSRAAIESLAENASDGIVAPAFWFLVGGLPGL
HHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHH
FAYKLINTADSMIGHLNDRYRDFGRFAAKLDDVANYIPARLTGLLAALATSLGHGREAGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
QALSIMCRDARLHRSPNAGWPEAAFAGALGLALAGPRQYGAETVEGPMLNATGKREAEAR
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHCCHHHCCCCCCCCCCCCCHHH
DIDAALVLFWSTMSLMTGLVIAASLVGLFVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]