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The map label for this gene is rutF [H]

Identifier: 23502168

GI number: 23502168

Start: 1252780

End: 1253301

Strand: Reverse

Name: rutF [H]

Synonym: BR1292

Alternate gene names: 23502168

Gene position: 1253301-1252780 (Counterclockwise)

Preceding gene: 23502170

Following gene: 23502167

Centisome position: 59.46

GC content: 56.7

Gene sequence:

>522_bases
GTGCAAACGGTAAATAACATCATCTCCTCCGTATCGACGGTGGAATCGAAAGCTTATCGCGACGCCATGAGCCATTATGC
GGGCGCGGTGCAGATCGTGACGACCGCCGGAGCAGCGGGGCGGCGCGGGCTGACGCTGACAGCGGCCTGCTCGGTATCGG
ACAATCCGCCGACAATCCTGATCTGTCTCCAGAAAATTCACGAAGAAAACCGCATTTTCATCGAAAATGGCGTTTTTGCC
ATCAATACGCTTGCCGGGCCGCATCAGCAGCTTGCCGATGCATTTTCAGGCCGTATCGGGCTGACACAGGATGAGCGTTT
TGAACTGGCGGCATGGGAGATACTGGCGACAGGCGCTCCGGTGCTGAAAGGCGCACTTGCCGCTTTCGATTGCCGTGTCG
TCAGTGTGCAGGATCATTCCACGCATCATGTGCTGTTCGGGGAGGTCGTCGGCCTCAGTTCCCATGCGGAAGAAGAGGCG
CTGATCTATCTCAACCGGCGTTATCACAAACTGGAACTCTGA

Upstream 100 bases:

>100_bases
GTGGGTAGCGGTTTCCTCGCCTTTGTGCAATGCCCGTAATGGATGCAGGCAATTGGGGTCAGCTCTGGTAACTGTCTGTG
GAATACGGGTCTGTGTTGAC

Downstream 100 bases:

>100_bases
CGCATGGGCAAGCGGGAAACAAAGCGCGCGATTAGCGGGCTTTTGAGCGCCTTTTTCCTTCTTCGCGTTTCATCCGCTTT
TGCCGGGGATATTTTGCCGC

Product: monooxygenase

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MQTVNNIISSVSTVESKAYRDAMSHYAGAVQIVTTAGAAGRRGLTLTAACSVSDNPPTILICLQKIHEENRIFIENGVFA
INTLAGPHQQLADAFSGRIGLTQDERFELAAWEILATGAPVLKGALAAFDCRVVSVQDHSTHHVLFGEVVGLSSHAEEEA
LIYLNRRYHKLEL

Sequences:

>Translated_173_residues
MQTVNNIISSVSTVESKAYRDAMSHYAGAVQIVTTAGAAGRRGLTLTAACSVSDNPPTILICLQKIHEENRIFIENGVFA
INTLAGPHQQLADAFSGRIGLTQDERFELAAWEILATGAPVLKGALAAFDCRVVSVQDHSTHHVLFGEVVGLSSHAEEEA
LIYLNRRYHKLEL
>Mature_173_residues
MQTVNNIISSVSTVESKAYRDAMSHYAGAVQIVTTAGAAGRRGLTLTAACSVSDNPPTILICLQKIHEENRIFIENGVFA
INTLAGPHQQLADAFSGRIGLTQDERFELAAWEILATGAPVLKGALAAFDCRVVSVQDHSTHHVLFGEVVGLSSHAEEEA
LIYLNRRYHKLEL

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=161, Percent_Identity=38.5093167701863, Blast_Score=128, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 18726; Mature: 18726

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTVNNIISSVSTVESKAYRDAMSHYAGAVQIVTTAGAAGRRGLTLTAACSVSDNPPTIL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEEEECCCCCCEEE
ICLQKIHEENRIFIENGVFAINTLAGPHQQLADAFSGRIGLTQDERFELAAWEILATGAP
EEEHHHCCCCEEEEECCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCH
VLKGALAAFDCRVVSVQDHSTHHVLFGEVVGLSSHAEEEALIYLNRRYHKLEL
HHHHHHHHHEEEEEEECCCCCEEEEEEEEECCCCCCCCCEEEEEECCEEEECC
>Mature Secondary Structure
MQTVNNIISSVSTVESKAYRDAMSHYAGAVQIVTTAGAAGRRGLTLTAACSVSDNPPTIL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEEEECCCCCCEEE
ICLQKIHEENRIFIENGVFAINTLAGPHQQLADAFSGRIGLTQDERFELAAWEILATGAP
EEEHHHCCCCEEEEECCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCH
VLKGALAAFDCRVVSVQDHSTHHVLFGEVVGLSSHAEEEALIYLNRRYHKLEL
HHHHHHHHHEEEEEEECCCCCEEEEEEEEECCCCCCCCCEEEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA