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The map label for this gene is dnaB [H]

Identifier: 23501351

GI number: 23501351

Start: 449962

End: 451467

Strand: Reverse

Name: dnaB [H]

Synonym: BR0450

Alternate gene names: 23501351

Gene position: 451467-449962 (Counterclockwise)

Preceding gene: 23501352

Following gene: 23501350

Centisome position: 21.42

GC content: 58.63

Gene sequence:

>1506_bases
ATGGCGGAAGCGGCGGTACGCAAACTCGACGATGCGCGGGACCAGAAGGACGTGCGCTATCGGGAGGCGCCCCACAATAT
TGAGGCAGAACAGGCGCTTCTCGGCGCTATTCTCATCAACAATGATGCATTCTACCGGGTTTCGGACTTTCTGAAACCGA
CCCATTTTCTTGAGCCCCTGCACCGGCGCATCTATGAGATCGCCACTGATCTCATCCGCGTCGGGAAAATGGCCAATCCG
GTGACGATGAAGACCTTCCTGCCGACCGAGGGCAAGGTGGGCGATCTTTCCATTTTCCAATATGTCACGCGCCTTGCGAC
CGAAGCCGTGACCATTATCAATGCCGAGGATTATGGCCGCGCGATCTATGATCTGGCGACGCGCCGCGCGCTGATCGGCA
TTGGCGAGGATATGGTCAACATCGCCTATGACGCGCCGGTGGATATGGCGCCGCAAGAGCAGATTGAAGACGCCGAACGC
CGCCTGTTCGAGCTTGCCGAAACGGGCCGCTATGATGGCGGCTTCCTGCCTTTCAAGGATGCGGTAACGACCGCCGTGGA
CATGGCCAACGCCGCCTTCATGCGCGACGGGCACCTTTCGGGCATTTCGACCGGCATTCACGCGCTTGATAGCAAGATGG
GCGGGTTGCAGCCATCGGACCTTATCATCCTTGCCGGGCGTCCTGGCATGGGCAAGACCTCGCTTGCCACCAATATCGCC
TTCAACATCGCCAATGCATACGAACCGGACCAGCAGGCCGACGGCACCATAAAGGCGGCCAATGGCGGCGTTGTCGGGTT
CTTCTCGCTCGAAATGTCTGCCGAACAGCTCGCCACCCGTATTATTTCCGAGCAAACGGAAGTGTCGTCCTCGAAAATCC
GCCGCGGCGAAATTACCGAAACGGATTTTGAAAAGCTCGTCGCCTGCTCGCAGGTCATGCAGAAGATCCCGCTCTATATC
GACCAGACAGGTGGCATTTCCATCGCGCAGCTTGCCGCGCGCGCACGCCGGTTGAAGCGCCAGCGCGGCCTCGACGTTCT
CGTCATCGACTATGTGCAGCTCATGACCGGCTCGTCCAAGGCTTCCGCGCAGAACCGCGTGCAGGAAATCACCGAAATCA
CCACCGGCCTGAAGGTGCTGGCGAAGGAACTCAATGTTCCGATCATCGCCCTGTCACAGCTTTCGCGCCAGGTGGAAAGC
CGCGACGACAAACGCCCGCAACTTTCCGACCTTCGCGAATCGGGCTCCATCGAGCAGGATGCCGACGTGGTGCTGTTCGT
GTTCCGCGAGGAATATTACGTCAAGAACCTCGAACCGCGCGACGAATTCGATCCGAAATACGAGGAATGGAAGCAGCATT
TCGAGAAAGTGCGCGGCACGGCCGACGTCATCATCGCCAAGCAGCGTCATGGGCCGACCGGTACGGTCAAGCTTGCCTTC
CAGTCCGAATTCACCCGGTTTGCTGATCTGGCCGATGGTTCCTATCTACCGGAGCAGTATGAATAA

Upstream 100 bases:

>100_bases
GGTATAGCTTGTAGCTTCCAGCAGGAATCAGCCGAAGCCGCGTTGCGTTTGGCTTCATTTAACGGCTAGCTGGAATCGAT
CAGGAACGGAAAAGAGAATC

Downstream 100 bases:

>100_bases
AATCATCATCACGACAAGGCTCGCCTGATGGCGAAGACGCGTGTTCAATTCATCTGCCAGAATTGCGGTGCTGTCCATTC
GCGCTGGGCGGGCAAGTGCG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MAEAAVRKLDDARDQKDVRYREAPHNIEAEQALLGAILINNDAFYRVSDFLKPTHFLEPLHRRIYEIATDLIRVGKMANP
VTMKTFLPTEGKVGDLSIFQYVTRLATEAVTIINAEDYGRAIYDLATRRALIGIGEDMVNIAYDAPVDMAPQEQIEDAER
RLFELAETGRYDGGFLPFKDAVTTAVDMANAAFMRDGHLSGISTGIHALDSKMGGLQPSDLIILAGRPGMGKTSLATNIA
FNIANAYEPDQQADGTIKAANGGVVGFFSLEMSAEQLATRIISEQTEVSSSKIRRGEITETDFEKLVACSQVMQKIPLYI
DQTGGISIAQLAARARRLKRQRGLDVLVIDYVQLMTGSSKASAQNRVQEITEITTGLKVLAKELNVPIIALSQLSRQVES
RDDKRPQLSDLRESGSIEQDADVVLFVFREEYYVKNLEPRDEFDPKYEEWKQHFEKVRGTADVIIAKQRHGPTGTVKLAF
QSEFTRFADLADGSYLPEQYE

Sequences:

>Translated_501_residues
MAEAAVRKLDDARDQKDVRYREAPHNIEAEQALLGAILINNDAFYRVSDFLKPTHFLEPLHRRIYEIATDLIRVGKMANP
VTMKTFLPTEGKVGDLSIFQYVTRLATEAVTIINAEDYGRAIYDLATRRALIGIGEDMVNIAYDAPVDMAPQEQIEDAER
RLFELAETGRYDGGFLPFKDAVTTAVDMANAAFMRDGHLSGISTGIHALDSKMGGLQPSDLIILAGRPGMGKTSLATNIA
FNIANAYEPDQQADGTIKAANGGVVGFFSLEMSAEQLATRIISEQTEVSSSKIRRGEITETDFEKLVACSQVMQKIPLYI
DQTGGISIAQLAARARRLKRQRGLDVLVIDYVQLMTGSSKASAQNRVQEITEITTGLKVLAKELNVPIIALSQLSRQVES
RDDKRPQLSDLRESGSIEQDADVVLFVFREEYYVKNLEPRDEFDPKYEEWKQHFEKVRGTADVIIAKQRHGPTGTVKLAF
QSEFTRFADLADGSYLPEQYE
>Mature_500_residues
AEAAVRKLDDARDQKDVRYREAPHNIEAEQALLGAILINNDAFYRVSDFLKPTHFLEPLHRRIYEIATDLIRVGKMANPV
TMKTFLPTEGKVGDLSIFQYVTRLATEAVTIINAEDYGRAIYDLATRRALIGIGEDMVNIAYDAPVDMAPQEQIEDAERR
LFELAETGRYDGGFLPFKDAVTTAVDMANAAFMRDGHLSGISTGIHALDSKMGGLQPSDLIILAGRPGMGKTSLATNIAF
NIANAYEPDQQADGTIKAANGGVVGFFSLEMSAEQLATRIISEQTEVSSSKIRRGEITETDFEKLVACSQVMQKIPLYID
QTGGISIAQLAARARRLKRQRGLDVLVIDYVQLMTGSSKASAQNRVQEITEITTGLKVLAKELNVPIIALSQLSRQVESR
DDKRPQLSDLRESGSIEQDADVVLFVFREEYYVKNLEPRDEFDPKYEEWKQHFEKVRGTADVIIAKQRHGPTGTVKLAFQ
SEFTRFADLADGSYLPEQYE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=491, Percent_Identity=34.4195519348269, Blast_Score=275, Evalue=4e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 55767; Mature: 55636

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAAVRKLDDARDQKDVRYREAPHNIEAEQALLGAILINNDAFYRVSDFLKPTHFLEPL
CCHHHHHHHHHHCCHHCCHHHCCCCCCCHHHHHHHHEEECCCCCCHHHHHCCHHHHHHHH
HRRIYEIATDLIRVGKMANPVTMKTFLPTEGKVGDLSIFQYVTRLATEAVTIINAEDYGR
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHEEEECCHHHHH
AIYDLATRRALIGIGEDMVNIAYDAPVDMAPQEQIEDAERRLFELAETGRYDGGFLPFKD
HHHHHHHHHHHHCCCHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
AVTTAVDMANAAFMRDGHLSGISTGIHALDSKMGGLQPSDLIILAGRPGMGKTSLATNIA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHH
FNIANAYEPDQQADGTIKAANGGVVGFFSLEMSAEQLATRIISEQTEVSSSKIRRGEITE
EEECCCCCCCCCCCCEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
TDFEKLVACSQVMQKIPLYIDQTGGISIAQLAARARRLKRQRGLDVLVIDYVQLMTGSSK
HHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCH
ASAQNRVQEITEITTGLKVLAKELNVPIIALSQLSRQVESRDDKRPQLSDLRESGSIEQD
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCHHHHHHCCCCCCC
ADVVLFVFREEYYVKNLEPRDEFDPKYEEWKQHFEKVRGTADVIIAKQRHGPTGTVKLAF
CCEEEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEE
QSEFTRFADLADGSYLPEQYE
HHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AEAAVRKLDDARDQKDVRYREAPHNIEAEQALLGAILINNDAFYRVSDFLKPTHFLEPL
CHHHHHHHHHHCCHHCCHHHCCCCCCCHHHHHHHHEEECCCCCCHHHHHCCHHHHHHHH
HRRIYEIATDLIRVGKMANPVTMKTFLPTEGKVGDLSIFQYVTRLATEAVTIINAEDYGR
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHEEEECCHHHHH
AIYDLATRRALIGIGEDMVNIAYDAPVDMAPQEQIEDAERRLFELAETGRYDGGFLPFKD
HHHHHHHHHHHHCCCHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
AVTTAVDMANAAFMRDGHLSGISTGIHALDSKMGGLQPSDLIILAGRPGMGKTSLATNIA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHH
FNIANAYEPDQQADGTIKAANGGVVGFFSLEMSAEQLATRIISEQTEVSSSKIRRGEITE
EEECCCCCCCCCCCCEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCH
TDFEKLVACSQVMQKIPLYIDQTGGISIAQLAARARRLKRQRGLDVLVIDYVQLMTGSSK
HHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCH
ASAQNRVQEITEITTGLKVLAKELNVPIIALSQLSRQVESRDDKRPQLSDLRESGSIEQD
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCHHHHHHCCCCCCC
ADVVLFVFREEYYVKNLEPRDEFDPKYEEWKQHFEKVRGTADVIIAKQRHGPTGTVKLAF
CCEEEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEE
QSEFTRFADLADGSYLPEQYE
HHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA