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The map label for this gene is radA [H]

Identifier: 23501350

GI number: 23501350

Start: 448531

End: 449934

Strand: Reverse

Name: radA [H]

Synonym: BR0449

Alternate gene names: 23501350

Gene position: 449934-448531 (Counterclockwise)

Preceding gene: 23501351

Following gene: 23501349

Centisome position: 21.35

GC content: 62.25

Gene sequence:

>1404_bases
ATGGCGAAGACGCGTGTTCAATTCATCTGCCAGAATTGCGGTGCTGTCCATTCGCGCTGGGCGGGCAAGTGCGATTCGTG
CGGCGAGTGGAATACACTCATCGAGGAAGGCACCAATAGCGGCATCGGTTCTGGCCCCGGCGCCATGCTTTCCAAGCGCA
AGGGCCGTACGGTGGCGCTGACTTCGCTTTCGGGCGAGATCGAGGATGCGCCGCGCATCATTTCCGGCATCAGCGAGCTT
GATCGCGTGACCGGCGGCGGCTTCGTGCGCGGTTCAGCCCTTCTGATCGGCGGCGATCCCGGCATCGGCAAGTCCACGCT
TTTGACACAGGCAGCCGCAGCCCTTTCCAATCGCGGCCATCGCATCGTCTATGTTTCGGGCGAGGAGGCGGTGGCACAGA
TACGCCTGCGCGCACAGCGCCTTGGCGTTGCCGCCTCGGCAGTGGAACTTGCCGCCGAAACCAATGTCGAAGATATCATC
GCCACCATTTCCAGCGATAATTCGGGCAGCAAACGGCCCGATCTCGTCATTATCGATTCGATCCAGACACTATGGACCGA
CATGGCTGATTCCGCGCCCGGAACGGTGACGCAGGTGCGCTCCTCGGCGCAAGCAATGATCCGCTATGCCAAGCAGACGG
GCGCTGCGGTGGTCCTGGTCGGCCATGTCACCAAGGACGGCCAGATTGCAGGCCCGCGCGTGGTGGAACACATGGTCGAT
GGCGTTCTCTATTTTGAGGGCGAAGGCGGGCACCATTATCGCATCCTGCGCACAGTGAAGAACCGCTTCGGGCCAACCGA
CGAAATCGGCGTGTTTGAAATGTCCGATGGCGGCCTGCGCGAGGTTTCCAACCCGTCCGAACTGTTTCTGGGTGAACGCA
ACGAAAAGTCGCCTGGCGCGGCGGTCTTTGCGGGCATGGAAGGCACGCGCCCCGTGCTGGTGGAAATCCAGGCGCTTGTC
GCCCCCTCCTCGCTTGGCACCCCGCGCCGCGCGGTGGTGGGCTGGGATGGCGGACGGCTTGCCATGATTCTCGCCGTACT
CGAATCGCATTGCGGTGTCCGCTTCGGCCAGCACGATGTCTATCTGAATGTGGCGGGCGGCTATCGCATCAGCGAGCCGG
CAGCCGATATAGCTGTGGCTGCGGCGCTGGTTTCCTCCATGGCCGGTATTGCCCTTCCGCCGGATTGCGTTTATTTCGGC
GAAATCAGCCTTTCCGGCGCTGTTCGCGCAGTCTCCCATGCGGTGCAGAGGCTCAAGGAAGCCGAAAAACTGGGCTTCCG
GCAGGCTGAGGTGCCGAATGGCAGCGGCGAGCTTTGGAAGGATCGCAATTTCCGCCTGATGGAAACGGCGGCCCTGGCCG
ATCTGGTGGCACGCATCGCAGCCTCGGGCGCTGGAAAGAAATAA

Upstream 100 bases:

>100_bases
TGCCTTCCAGTCCGAATTCACCCGGTTTGCTGATCTGGCCGATGGTTCCTATCTACCGGAGCAGTATGAATAAAATCATC
ATCACGACAAGGCTCGCCTG

Downstream 100 bases:

>100_bases
CACTTCAGGGGCATGCACGGGAAGATTCTATCAACCTTCCAATGTTAGGCTCCCGCATAATTGACAATTTGACGGTCCCC
GTGCACCGAATGTTCGGGCG

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MAKTRVQFICQNCGAVHSRWAGKCDSCGEWNTLIEEGTNSGIGSGPGAMLSKRKGRTVALTSLSGEIEDAPRIISGISEL
DRVTGGGFVRGSALLIGGDPGIGKSTLLTQAAAALSNRGHRIVYVSGEEAVAQIRLRAQRLGVAASAVELAAETNVEDII
ATISSDNSGSKRPDLVIIDSIQTLWTDMADSAPGTVTQVRSSAQAMIRYAKQTGAAVVLVGHVTKDGQIAGPRVVEHMVD
GVLYFEGEGGHHYRILRTVKNRFGPTDEIGVFEMSDGGLREVSNPSELFLGERNEKSPGAAVFAGMEGTRPVLVEIQALV
APSSLGTPRRAVVGWDGGRLAMILAVLESHCGVRFGQHDVYLNVAGGYRISEPAADIAVAAALVSSMAGIALPPDCVYFG
EISLSGAVRAVSHAVQRLKEAEKLGFRQAEVPNGSGELWKDRNFRLMETAALADLVARIAASGAGKK

Sequences:

>Translated_467_residues
MAKTRVQFICQNCGAVHSRWAGKCDSCGEWNTLIEEGTNSGIGSGPGAMLSKRKGRTVALTSLSGEIEDAPRIISGISEL
DRVTGGGFVRGSALLIGGDPGIGKSTLLTQAAAALSNRGHRIVYVSGEEAVAQIRLRAQRLGVAASAVELAAETNVEDII
ATISSDNSGSKRPDLVIIDSIQTLWTDMADSAPGTVTQVRSSAQAMIRYAKQTGAAVVLVGHVTKDGQIAGPRVVEHMVD
GVLYFEGEGGHHYRILRTVKNRFGPTDEIGVFEMSDGGLREVSNPSELFLGERNEKSPGAAVFAGMEGTRPVLVEIQALV
APSSLGTPRRAVVGWDGGRLAMILAVLESHCGVRFGQHDVYLNVAGGYRISEPAADIAVAAALVSSMAGIALPPDCVYFG
EISLSGAVRAVSHAVQRLKEAEKLGFRQAEVPNGSGELWKDRNFRLMETAALADLVARIAASGAGKK
>Mature_466_residues
AKTRVQFICQNCGAVHSRWAGKCDSCGEWNTLIEEGTNSGIGSGPGAMLSKRKGRTVALTSLSGEIEDAPRIISGISELD
RVTGGGFVRGSALLIGGDPGIGKSTLLTQAAAALSNRGHRIVYVSGEEAVAQIRLRAQRLGVAASAVELAAETNVEDIIA
TISSDNSGSKRPDLVIIDSIQTLWTDMADSAPGTVTQVRSSAQAMIRYAKQTGAAVVLVGHVTKDGQIAGPRVVEHMVDG
VLYFEGEGGHHYRILRTVKNRFGPTDEIGVFEMSDGGLREVSNPSELFLGERNEKSPGAAVFAGMEGTRPVLVEIQALVA
PSSLGTPRRAVVGWDGGRLAMILAVLESHCGVRFGQHDVYLNVAGGYRISEPAADIAVAAALVSSMAGIALPPDCVYFGE
ISLSGAVRAVSHAVQRLKEAEKLGFRQAEVPNGSGELWKDRNFRLMETAALADLVARIAASGAGKK

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=430, Percent_Identity=44.1860465116279, Blast_Score=371, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR020588
- InterPro:   IPR004504
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC [H]

EC number: NA

Molecular weight: Translated: 49054; Mature: 48923

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTRVQFICQNCGAVHSRWAGKCDSCGEWNTLIEEGTNSGIGSGPGAMLSKRKGRTVAL
CCCHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEE
TSLSGEIEDAPRIISGISELDRVTGGGFVRGSALLIGGDPGIGKSTLLTQAAAALSNRGH
EECCCCCCHHHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCHHHHHHHHHHHHHCCCC
RIVYVSGEEAVAQIRLRAQRLGVAASAVELAAETNVEDIIATISSDNSGSKRPDLVIIDS
EEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEECC
IQTLWTDMADSAPGTVTQVRSSAQAMIRYAKQTGAAVVLVGHVTKDGQIAGPRVVEHMVD
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHC
GVLYFEGEGGHHYRILRTVKNRFGPTDEIGVFEMSDGGLREVSNPSELFLGERNEKSPGA
CEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHEECCCCCCCCCC
AVFAGMEGTRPVLVEIQALVAPSSLGTPRRAVVGWDGGRLAMILAVLESHCGVRFGQHDV
EEEECCCCCCCEEEEEEEHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEECCCEE
YLNVAGGYRISEPAADIAVAAALVSSMAGIALPPDCVYFGEISLSGAVRAVSHAVQRLKE
EEEECCCEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEECEEHHHHHHHHHHHHHHHHH
AEKLGFRQAEVPNGSGELWKDRNFRLMETAALADLVARIAASGAGKK
HHHCCCEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AKTRVQFICQNCGAVHSRWAGKCDSCGEWNTLIEEGTNSGIGSGPGAMLSKRKGRTVAL
CCHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEE
TSLSGEIEDAPRIISGISELDRVTGGGFVRGSALLIGGDPGIGKSTLLTQAAAALSNRGH
EECCCCCCHHHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCCHHHHHHHHHHHHHCCCC
RIVYVSGEEAVAQIRLRAQRLGVAASAVELAAETNVEDIIATISSDNSGSKRPDLVIIDS
EEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEECC
IQTLWTDMADSAPGTVTQVRSSAQAMIRYAKQTGAAVVLVGHVTKDGQIAGPRVVEHMVD
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHC
GVLYFEGEGGHHYRILRTVKNRFGPTDEIGVFEMSDGGLREVSNPSELFLGERNEKSPGA
CEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHEECCCCCCCCCC
AVFAGMEGTRPVLVEIQALVAPSSLGTPRRAVVGWDGGRLAMILAVLESHCGVRFGQHDV
EEEECCCCCCCEEEEEEEHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEECCCEE
YLNVAGGYRISEPAADIAVAAALVSSMAGIALPPDCVYFGEISLSGAVRAVSHAVQRLKE
EEEECCCEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEECEEHHHHHHHHHHHHHHHHH
AEKLGFRQAEVPNGSGELWKDRNFRLMETAALADLVARIAASGAGKK
HHHCCCEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]