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The map label for this gene is nhaA [H]

Identifier: 23501301

GI number: 23501301

Start: 401319

End: 402500

Strand: Reverse

Name: nhaA [H]

Synonym: BR0397

Alternate gene names: 23501301

Gene position: 402500-401319 (Counterclockwise)

Preceding gene: 23501302

Following gene: 23501300

Centisome position: 19.1

GC content: 59.48

Gene sequence:

>1182_bases
ATGAATCATAGCCCGCAATCCGCTCGCCCCGTTTCCATCATGCGACGTTTTCTGGACAGCGAAGCCGCTGGCGGCATAAC
GCTCATGGCTGCAGCGGCGCTTGCCCTCATCGTTGCAAATTCGCCCTTTGCCCAGACCTATTTCGATGCGTTGCATCTAT
ATATCGGCCCGCTCAGCCTTGCGCACTGGATCAACGATGCGCTGATGGCAATATTCTTTTTGCTGGTGGGACTGGAAATC
AAGCGCGAGATGCTGGACGGGCAGCTCGCAAGCTGGCCCAACCGCATGTTGCCGGGCATTGCGGCAGCGGGCGGCGTCAT
TTTGCCTGCAATCATTTTCGCGGTTCTCAACCATGACAATCCGGCCAAGCTGCGCGGCTGGGCGGTGCCATCGGCAACCG
ATATCGCCTTTGCGCTCGGCGTACTGTCGCTTCTGGGTTCGCGCGCGCCGTCAAGCCTCAAGGTGTTTCTGGCAACGCTT
GCCATTCTTGACGACCTTGCCGCCGTGGTCATCATCGCAATCTTCTACACGGCTGAAATTTCCATGCCCTATCTGGGCGC
GGCCTTTATCACCGCCGCGGTCCTCTTTGTCATGAACCGCATGGGCGTGGTGAAGCTCCTGCCTTATCTGATCAGCGCTG
TGATCCTCTGGTTCTTCGTCTTCAATTCCGGCGTTCATGCCACGGTTGCCGGTGTTGTCGCAGCCCTCATGATCCCGCTA
AAACCTGCACCCGGCAGACCCGACGACATGACCTCGCCCCTGCACAAGCTGGAACATGCACTGGCCAAGCCCGTTGCCTT
CATCGTCGTGCCGATCTTCGGCTTTGCCAATGCGGGCATTTCCTTCAAGGGGCTTGAGGCTTCCGTCCTGGGCGACACGC
TGACACTCGGCATCCTGCTCGGCCTTTTCCTCGGCAAGCAGTTCGGCGTCTTCGGCGCGGCCTGGCTCGCCATCAAAACG
GGGCTTGCCGAAAAGCCGATGGGCGCAAGCTGGGTTCAGCTTTACGGCGTCGCCATTCTTTGCGGCATCGGCTTTACGAT
GAGCATCTTCATCGGCCTTCTCTCCTTCCCGTCCGACCTCATGCAGACGGAAACGAAGATCGGTGTCCTTTCCGGCTCCG
CCCTTTCGGCCATTTGCGGCTATCTCCTGTTGCGGGCGGCGGCCAGACCAAAGCGCGGCTAA

Upstream 100 bases:

>100_bases
ATCTCTGGGACTGAAATTTTTGTTCTGCCTTTCCGGCCCGTTTTTATTCCAACTGTGCCGAAGGTCATCCTTAGACGATA
AAAACAATAAACGGAAACCG

Downstream 100 bases:

>100_bases
CCCGCTCTGGAAAGCTGATGAGTCGCCCGAAGCGAAAAATTTCGGGCGATTTCTTTGTGTTTTCCACTTTGCTTCCTACA
TTGCATCCACATATTGCACG

Product: pH-dependent sodium/proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MNHSPQSARPVSIMRRFLDSEAAGGITLMAAAALALIVANSPFAQTYFDALHLYIGPLSLAHWINDALMAIFFLLVGLEI
KREMLDGQLASWPNRMLPGIAAAGGVILPAIIFAVLNHDNPAKLRGWAVPSATDIAFALGVLSLLGSRAPSSLKVFLATL
AILDDLAAVVIIAIFYTAEISMPYLGAAFITAAVLFVMNRMGVVKLLPYLISAVILWFFVFNSGVHATVAGVVAALMIPL
KPAPGRPDDMTSPLHKLEHALAKPVAFIVVPIFGFANAGISFKGLEASVLGDTLTLGILLGLFLGKQFGVFGAAWLAIKT
GLAEKPMGASWVQLYGVAILCGIGFTMSIFIGLLSFPSDLMQTETKIGVLSGSALSAICGYLLLRAAARPKRG

Sequences:

>Translated_393_residues
MNHSPQSARPVSIMRRFLDSEAAGGITLMAAAALALIVANSPFAQTYFDALHLYIGPLSLAHWINDALMAIFFLLVGLEI
KREMLDGQLASWPNRMLPGIAAAGGVILPAIIFAVLNHDNPAKLRGWAVPSATDIAFALGVLSLLGSRAPSSLKVFLATL
AILDDLAAVVIIAIFYTAEISMPYLGAAFITAAVLFVMNRMGVVKLLPYLISAVILWFFVFNSGVHATVAGVVAALMIPL
KPAPGRPDDMTSPLHKLEHALAKPVAFIVVPIFGFANAGISFKGLEASVLGDTLTLGILLGLFLGKQFGVFGAAWLAIKT
GLAEKPMGASWVQLYGVAILCGIGFTMSIFIGLLSFPSDLMQTETKIGVLSGSALSAICGYLLLRAAARPKRG
>Mature_393_residues
MNHSPQSARPVSIMRRFLDSEAAGGITLMAAAALALIVANSPFAQTYFDALHLYIGPLSLAHWINDALMAIFFLLVGLEI
KREMLDGQLASWPNRMLPGIAAAGGVILPAIIFAVLNHDNPAKLRGWAVPSATDIAFALGVLSLLGSRAPSSLKVFLATL
AILDDLAAVVIIAIFYTAEISMPYLGAAFITAAVLFVMNRMGVVKLLPYLISAVILWFFVFNSGVHATVAGVVAALMIPL
KPAPGRPDDMTSPLHKLEHALAKPVAFIVVPIFGFANAGISFKGLEASVLGDTLTLGILLGLFLGKQFGVFGAAWLAIKT
GLAEKPMGASWVQLYGVAILCGIGFTMSIFIGLLSFPSDLMQTETKIGVLSGSALSAICGYLLLRAAARPKRG

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons [H]

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1786201, Length=389, Percent_Identity=48.0719794344473, Blast_Score=303, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023171
- InterPro:   IPR004670
- InterPro:   IPR002142 [H]

Pfam domain/function: PF06965 Na_H_antiport_1; PF01343 Peptidase_S49 [H]

EC number: NA

Molecular weight: Translated: 41443; Mature: 41443

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSPQSARPVSIMRRFLDSEAAGGITLMAAAALALIVANSPFAQTYFDALHLYIGPLSL
CCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AHWINDALMAIFFLLVGLEIKREMLDGQLASWPNRMLPGIAAAGGVILPAIIFAVLNHDN
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHCCCHHHCCHHHHHHHHHHHHCCCC
PAKLRGWAVPSATDIAFALGVLSLLGSRAPSSLKVFLATLAILDDLAAVVIIAIFYTAEI
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SMPYLGAAFITAAVLFVMNRMGVVKLLPYLISAVILWFFVFNSGVHATVAGVVAALMIPL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
KPAPGRPDDMTSPLHKLEHALAKPVAFIVVPIFGFANAGISFKGLEASVLGDTLTLGILL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHH
GLFLGKQFGVFGAAWLAIKTGLAEKPMGASWVQLYGVAILCGIGFTMSIFIGLLSFPSDL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
MQTETKIGVLSGSALSAICGYLLLRAAARPKRG
HHHHHHHHEECCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure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HHHHHHHHEECCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA