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The map label for this gene is nhaA [H]

Identifier: 23501300

GI number: 23501300

Start: 400292

End: 401398

Strand: Reverse

Name: nhaA [H]

Synonym: BR0396

Alternate gene names: 23501300

Gene position: 401398-400292 (Counterclockwise)

Preceding gene: 23501301

Following gene: 23501299

Centisome position: 19.04

GC content: 59.98

Gene sequence:

>1107_bases
GTGTCCTTTCCGGCTCCGCCCTTTCGGCCATTTGCGGCTATCTCCTGTTGCGGGCGGCGGCCAGACCAAAGCGCGGCTAA
CCCGCTCTGGAAAGCTGATGAGTCGCCCGAAGCGAAAAATTTCGGGCGATTTCTTTGTGTTTTCCACTTTGCTTCCTACA
TTGCATCCACATATTGCACGGAGCGGTTGCAAGCCGCTTCATCCCTTTTGCGCCGCTCAAGTTTGGAGTTCGCCTTGCCC
GGTCTGTTGAAACGCCTTATTCCCCGCCGTTTTCGCGCCGTGGAAACGGAAATCCCCGTCGTGCGCCTGCATGGCGCAAT
CATGACCGGCGGCACATCCTTGCGGCCCACGCTTTCGCTCGCATCGACGGCCGGCATTCTGGAAAAGGCCTTTGCCGACA
AGCATGCCCCTGCGGTTGCCATATCCATCAATTCGCCGGGCGGCGCGCCGGTGCAATCGCGCCTGATCTATCGCCGCATC
CGCGATCTTGCCGTGGAACATCAAAAGAAGGTTTTCGTCTTCGTGGAAGATGTGGCGGCCTCCGGCGGCTACATGATCGC
GCTTGCGGGCGACGAAATCATCGCCGACCCCTCCTCCATCGTTGGCTCCATCGGCGTCGTTTCGGCCTCTTTCGGCTTTC
CCGAATTGCTGAAGAAGATCGGTGTGGAGCGGCGTGTCTATACGGCGGGCAGCAACAAGGTGACACTCGATCCGTTCCAG
CCGGAAAAGGCCGAGGACATCGAACGGCTGAAAGCACTTCAGCTTGAAATCCACGCCACCTTCATCGACATGGTGAAGGA
GCGGCGCGCCGGCAAGCTTGGCGACAATCCCGATCTTTTCAGCGGCCTGTTCTGGACCGGCACCACCGCCGCCTCGCTCG
GCCTCATCGACGGGCTTGGCGACATGCGCAGTTTTCTGCGCAAGACCTATGGCGACAAGGTGAAACTGAAGCTCATCCAG
CCGCAACGCGGCCTTCTGGGCCGCAAGCTGCCCGGCATCGGCATGGATTCAGGCAGTGTCGAGCCAGCGCAAATCGCCGC
CCATCTTGGCGACGGGCTGCTTTGTGTCGCGGAAGAAAAAGCAATCTGGGCACGCTATGGGCTTTGA

Upstream 100 bases:

>100_bases
TACGGCGTCGCCATTCTTTGCGGCATCGGCTTTACGATGAGCATCTTCATCGGCCTTCTCTCCTTCCCGTCCGACCTCAT
GCAGACGGAAACGAAGATCG

Downstream 100 bases:

>100_bases
ACTCCATGGATTTACCATGATATGCTGCCACAAATGAGAGGATGGAGGGGCGCTCCTGGTGGATTGAATTTGACGCTTGC
TCGGCCCCCCTATCCGAGGG

Product: protease IV

Products: NA

Alternate protein names: Sodium/proton antiporter nhaA [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSFPAPPFRPFAAISCCGRRPDQSAANPLWKADESPEAKNFGRFLCVFHFASYIASTYCTERLQAASSLLRRSSLEFALP
GLLKRLIPRRFRAVETEIPVVRLHGAIMTGGTSLRPTLSLASTAGILEKAFADKHAPAVAISINSPGGAPVQSRLIYRRI
RDLAVEHQKKVFVFVEDVAASGGYMIALAGDEIIADPSSIVGSIGVVSASFGFPELLKKIGVERRVYTAGSNKVTLDPFQ
PEKAEDIERLKALQLEIHATFIDMVKERRAGKLGDNPDLFSGLFWTGTTAASLGLIDGLGDMRSFLRKTYGDKVKLKLIQ
PQRGLLGRKLPGIGMDSGSVEPAQIAAHLGDGLLCVAEEKAIWARYGL

Sequences:

>Translated_368_residues
MSFPAPPFRPFAAISCCGRRPDQSAANPLWKADESPEAKNFGRFLCVFHFASYIASTYCTERLQAASSLLRRSSLEFALP
GLLKRLIPRRFRAVETEIPVVRLHGAIMTGGTSLRPTLSLASTAGILEKAFADKHAPAVAISINSPGGAPVQSRLIYRRI
RDLAVEHQKKVFVFVEDVAASGGYMIALAGDEIIADPSSIVGSIGVVSASFGFPELLKKIGVERRVYTAGSNKVTLDPFQ
PEKAEDIERLKALQLEIHATFIDMVKERRAGKLGDNPDLFSGLFWTGTTAASLGLIDGLGDMRSFLRKTYGDKVKLKLIQ
PQRGLLGRKLPGIGMDSGSVEPAQIAAHLGDGLLCVAEEKAIWARYGL
>Mature_367_residues
SFPAPPFRPFAAISCCGRRPDQSAANPLWKADESPEAKNFGRFLCVFHFASYIASTYCTERLQAASSLLRRSSLEFALPG
LLKRLIPRRFRAVETEIPVVRLHGAIMTGGTSLRPTLSLASTAGILEKAFADKHAPAVAISINSPGGAPVQSRLIYRRIR
DLAVEHQKKVFVFVEDVAASGGYMIALAGDEIIADPSSIVGSIGVVSASFGFPELLKKIGVERRVYTAGSNKVTLDPFQP
EKAEDIERLKALQLEIHATFIDMVKERRAGKLGDNPDLFSGLFWTGTTAASLGLIDGLGDMRSFLRKTYGDKVKLKLIQP
QRGLLGRKLPGIGMDSGSVEPAQIAAHLGDGLLCVAEEKAIWARYGL

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1787527, Length=167, Percent_Identity=34.7305389221557, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788064, Length=214, Percent_Identity=31.3084112149533, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023171
- InterPro:   IPR004670
- InterPro:   IPR002142 [H]

Pfam domain/function: PF06965 Na_H_antiport_1; PF01343 Peptidase_S49 [H]

EC number: 3.4.21.- [C]

Molecular weight: Translated: 39867; Mature: 39735

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFPAPPFRPFAAISCCGRRPDQSAANPLWKADESPEAKNFGRFLCVFHFASYIASTYCT
CCCCCCCCCHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ERLQAASSLLRRSSLEFALPGLLKRLIPRRFRAVETEIPVVRLHGAIMTGGTSLRPTLSL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCCCHHHH
ASTAGILEKAFADKHAPAVAISINSPGGAPVQSRLIYRRIRDLAVEHQKKVFVFVEDVAA
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
SGGYMIALAGDEIIADPSSIVGSIGVVSASFGFPELLKKIGVERRVYTAGSNKVTLDPFQ
CCCEEEEEECCCCCCCCHHHHHHHHHEECCCCCHHHHHHCCCCCEEEECCCCEEEECCCC
PEKAEDIERLKALQLEIHATFIDMVKERRAGKLGDNPDLFSGLFWTGTTAASLGLIDGLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCHHCCCCHHHHHHHHHCHH
DMRSFLRKTYGDKVKLKLIQPQRGLLGRKLPGIGMDSGSVEPAQIAAHLGDGLLCVAEEK
HHHHHHHHHCCCEEEEEEECCCCCHHCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCC
AIWARYGL
HHHHHCCC
>Mature Secondary Structure 
SFPAPPFRPFAAISCCGRRPDQSAANPLWKADESPEAKNFGRFLCVFHFASYIASTYCT
CCCCCCCCHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ERLQAASSLLRRSSLEFALPGLLKRLIPRRFRAVETEIPVVRLHGAIMTGGTSLRPTLSL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCCCHHHH
ASTAGILEKAFADKHAPAVAISINSPGGAPVQSRLIYRRIRDLAVEHQKKVFVFVEDVAA
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEECC
SGGYMIALAGDEIIADPSSIVGSIGVVSASFGFPELLKKIGVERRVYTAGSNKVTLDPFQ
CCCEEEEEECCCCCCCCHHHHHHHHHEECCCCCHHHHHHCCCCCEEEECCCCEEEECCCC
PEKAEDIERLKALQLEIHATFIDMVKERRAGKLGDNPDLFSGLFWTGTTAASLGLIDGLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCHHCCCCHHHHHHHHHCHH
DMRSFLRKTYGDKVKLKLIQPQRGLLGRKLPGIGMDSGSVEPAQIAAHLGDGLLCVAEEK
HHHHHHHHHCCCEEEEEEECCCCCHHCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCC
AIWARYGL
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA