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The map label for this gene is yeiT [H]

Identifier: 23501190

GI number: 23501190

Start: 291402

End: 292907

Strand: Reverse

Name: yeiT [H]

Synonym: BR0283

Alternate gene names: 23501190

Gene position: 292907-291402 (Counterclockwise)

Preceding gene: 23501191

Following gene: 23501189

Centisome position: 13.9

GC content: 60.82

Gene sequence:

>1506_bases
ATGCGATCCATGAAACACACGGGATCAGCAGGAAGTTCGTCCATTGATGCAGGCTCTCCAGACATTCGCGGCGCGCGGCT
TCCTGCTGGCGATTATGCGCATGTCTTCGACGACCTGCATCCACCGCTCTCACCGCATGAAGCCATTGTCGAAAGCGACC
GCTGTTATTTCTGTTATGACGCGCCTTGCATGAACGCCTGCCCGACGGGCATCGATATTCCGCTTTTCATCCGGCAGATC
AATGCAGGCAACCCGGCTGGCGCGGCCAGGACCATTCTTGCCGAAAACATTCTGGGCGGCATGTGCGCCCGCGTCTGCCC
GACCGAAACGCTCTGCGAGGAGGTCTGTGTGCGCGAGGTGGCGGAAGGCAAGCCGGTGAAGATCGGCCATTTGCAACGCT
ACGCTACCGATATGCTGATGGCCACGGGCAACCACCCGTTTACCCGCGCAGCCGAAACGGGCAGGCACATTGCCGTTGTC
GGCGCTGGCCCGGCGGGCATTTCGGCTGCACATCGCCTTGCCATGCATGGCCATCAGGTGACGATTTTCGAGGCCCGGCC
CAAGGGCGGCGGTCTCAACGAATATGGCATCGCCGCCTATAAGACGGTGAACAATTTCGCCCAGCGCGAACTTGATTTCG
TGTTGCAGATCGGCGGCATCCATATCGAATATAACCAGACGCTGGGCCAGGACATTACCGTCGAGGCGCTGAAGGCCGGA
TATGATGCGGTGTTCCTCGCCATGGGGCTGCCGGGCGTGAACGACCTCGGCCTTGCCGGCGAAGACGCGCCGAACGTGCT
GGATGCCGTCGATTACATCGCCAATCTGCGGCAGGCCAGGAACCTTTCCACCCTGCCCGTCGGGCGCGATATCGTCGTTA
TCGGTGGCGGCATGACGGCCATCGACGTTGCAATCCAGACCAAAAAGCTCGGCGCAAACAACGTCACCATCGTCTATCGC
CGCGGACAGGAAAGCATGAATGCCAGCGCCTATGAACAGGAACTGGCGCAGATACATGGCGTCGTCATCCGCCACTGGTT
GCAGCCCCATGCGCTGGAGCGCAGTGAAGACGGCATCGTCCATGCGATCACCTTTGAATATACGGCCAATCGGGATGGCA
AGCTTGTCGGCACCGGCGAATATCTGACCCTTGAGGCCGACCAGGTGTTCAAGGCCATCGGCCAGACCTTCGATCCGGAA
CCCTTGCGCGGGTCCGGCATCGCCCTTGCGAAAGGCCGCATCAGCGTGGATGCGGAAGGGCGGACCTCGGTGGAGGGTAT
CTGGGCTGGCGGCGATTGTGTGGCAGGCGGAAAAGATCTGACGGTTGCCTCCGTTGAAGACGGCAAGGTCGCGGCCTTAT
CCATTCATGCGGCACTGACGAGCCGCCCGGAAGCTGTGGAAGGTTTTGCCGAAGCCGTTCTTTCCGGTGCCGCGCATTAT
GCGCCCGCAGCGCCATCCCGTCACGCAACCGTTCAACCTGGCAGCGAACAGGGTTTGGGAGATTGA

Upstream 100 bases:

>100_bases
TGTCTGTAACGGGCCATAAGCGGCAATAAAGATCGCAGCCCGGAAAAACAGCAGCTTTGCGCAAAGGGAACCCGCATGGC
TGCATCAGCAAAAATGGGAG

Downstream 100 bases:

>100_bases
AAAATGGCTGATCTTTCAACGAATTTCCTCGGCATCAAATCACCCAATCCGTTCTGGCTCGCCTCGGCCCCGCCGACCGA
CAAGGCCTATAATGTCGAGC

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MRSMKHTGSAGSSSIDAGSPDIRGARLPAGDYAHVFDDLHPPLSPHEAIVESDRCYFCYDAPCMNACPTGIDIPLFIRQI
NAGNPAGAARTILAENILGGMCARVCPTETLCEEVCVREVAEGKPVKIGHLQRYATDMLMATGNHPFTRAAETGRHIAVV
GAGPAGISAAHRLAMHGHQVTIFEARPKGGGLNEYGIAAYKTVNNFAQRELDFVLQIGGIHIEYNQTLGQDITVEALKAG
YDAVFLAMGLPGVNDLGLAGEDAPNVLDAVDYIANLRQARNLSTLPVGRDIVVIGGGMTAIDVAIQTKKLGANNVTIVYR
RGQESMNASAYEQELAQIHGVVIRHWLQPHALERSEDGIVHAITFEYTANRDGKLVGTGEYLTLEADQVFKAIGQTFDPE
PLRGSGIALAKGRISVDAEGRTSVEGIWAGGDCVAGGKDLTVASVEDGKVAALSIHAALTSRPEAVEGFAEAVLSGAAHY
APAAPSRHATVQPGSEQGLGD

Sequences:

>Translated_501_residues
MRSMKHTGSAGSSSIDAGSPDIRGARLPAGDYAHVFDDLHPPLSPHEAIVESDRCYFCYDAPCMNACPTGIDIPLFIRQI
NAGNPAGAARTILAENILGGMCARVCPTETLCEEVCVREVAEGKPVKIGHLQRYATDMLMATGNHPFTRAAETGRHIAVV
GAGPAGISAAHRLAMHGHQVTIFEARPKGGGLNEYGIAAYKTVNNFAQRELDFVLQIGGIHIEYNQTLGQDITVEALKAG
YDAVFLAMGLPGVNDLGLAGEDAPNVLDAVDYIANLRQARNLSTLPVGRDIVVIGGGMTAIDVAIQTKKLGANNVTIVYR
RGQESMNASAYEQELAQIHGVVIRHWLQPHALERSEDGIVHAITFEYTANRDGKLVGTGEYLTLEADQVFKAIGQTFDPE
PLRGSGIALAKGRISVDAEGRTSVEGIWAGGDCVAGGKDLTVASVEDGKVAALSIHAALTSRPEAVEGFAEAVLSGAAHY
APAAPSRHATVQPGSEQGLGD
>Mature_501_residues
MRSMKHTGSAGSSSIDAGSPDIRGARLPAGDYAHVFDDLHPPLSPHEAIVESDRCYFCYDAPCMNACPTGIDIPLFIRQI
NAGNPAGAARTILAENILGGMCARVCPTETLCEEVCVREVAEGKPVKIGHLQRYATDMLMATGNHPFTRAAETGRHIAVV
GAGPAGISAAHRLAMHGHQVTIFEARPKGGGLNEYGIAAYKTVNNFAQRELDFVLQIGGIHIEYNQTLGQDITVEALKAG
YDAVFLAMGLPGVNDLGLAGEDAPNVLDAVDYIANLRQARNLSTLPVGRDIVVIGGGMTAIDVAIQTKKLGANNVTIVYR
RGQESMNASAYEQELAQIHGVVIRHWLQPHALERSEDGIVHAITFEYTANRDGKLVGTGEYLTLEADQVFKAIGQTFDPE
PLRGSGIALAKGRISVDAEGRTSVEGIWAGGDCVAGGKDLTVASVEDGKVAALSIHAALTSRPEAVEGFAEAVLSGAAHY
APAAPSRHATVQPGSEQGLGD

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=467, Percent_Identity=32.9764453961456, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI237757300, Length=106, Percent_Identity=44.3396226415094, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1788811, Length=446, Percent_Identity=34.5291479820628, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1788468, Length=435, Percent_Identity=35.1724137931034, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI87082180, Length=480, Percent_Identity=34.5833333333333, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1789606, Length=453, Percent_Identity=32.2295805739514, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789243, Length=449, Percent_Identity=29.6213808463252, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71984108, Length=453, Percent_Identity=34.878587196468, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17570289, Length=272, Percent_Identity=28.6764705882353, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17543860, Length=156, Percent_Identity=32.0512820512821, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=461, Percent_Identity=24.9457700650759, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24640763, Length=464, Percent_Identity=33.4051724137931, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI18858217, Length=464, Percent_Identity=33.4051724137931, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24665547, Length=476, Percent_Identity=26.890756302521, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24665543, Length=476, Percent_Identity=26.890756302521, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28574881, Length=476, Percent_Identity=26.890756302521, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24665539, Length=476, Percent_Identity=26.890756302521, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 52811; Mature: 52811

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSMKHTGSAGSSSIDAGSPDIRGARLPAGDYAHVFDDLHPPLSPHEAIVESDRCYFCYD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHCCCCEEEEEC
APCMNACPTGIDIPLFIRQINAGNPAGAARTILAENILGGMCARVCPTETLCEEVCVREV
CCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AEGKPVKIGHLQRYATDMLMATGNHPFTRAAETGRHIAVVGAGPAGISAAHRLAMHGHQV
HCCCCEEEHHHHHHHHHHHEECCCCCCHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEE
TIFEARPKGGGLNEYGIAAYKTVNNFAQRELDFVLQIGGIHIEYNQTLGQDITVEALKAG
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCEEEEECCCCCCCEEHHHHHCC
YDAVFLAMGLPGVNDLGLAGEDAPNVLDAVDYIANLRQARNLSTLPVGRDIVVIGGGMTA
CCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEE
IDVAIQTKKLGANNVTIVYRRGQESMNASAYEQELAQIHGVVIRHWLQPHALERSEDGIV
EEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEE
HAITFEYTANRDGKLVGTGEYLTLEADQVFKAIGQTFDPEPLRGSGIALAKGRISVDAEG
EEEEEEEECCCCCCEEECCCEEEEEHHHHHHHHCCCCCCCCCCCCCEEEECCEEEECCCC
RTSVEGIWAGGDCVAGGKDLTVASVEDGKVAALSIHAALTSRPEAVEGFAEAVLSGAAHY
CCCCCEEEECCCEECCCCCEEEEEECCCCEEEEEEEEHHCCCCHHHHHHHHHHHHCCHHC
APAAPSRHATVQPGSEQGLGD
CCCCCCCCCEECCCCCCCCCC
>Mature Secondary Structure
MRSMKHTGSAGSSSIDAGSPDIRGARLPAGDYAHVFDDLHPPLSPHEAIVESDRCYFCYD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHCCCCEEEEEC
APCMNACPTGIDIPLFIRQINAGNPAGAARTILAENILGGMCARVCPTETLCEEVCVREV
CCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AEGKPVKIGHLQRYATDMLMATGNHPFTRAAETGRHIAVVGAGPAGISAAHRLAMHGHQV
HCCCCEEEHHHHHHHHHHHEECCCCCCHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCEE
TIFEARPKGGGLNEYGIAAYKTVNNFAQRELDFVLQIGGIHIEYNQTLGQDITVEALKAG
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCEEEEECCCCCCCEEHHHHHCC
YDAVFLAMGLPGVNDLGLAGEDAPNVLDAVDYIANLRQARNLSTLPVGRDIVVIGGGMTA
CCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEE
IDVAIQTKKLGANNVTIVYRRGQESMNASAYEQELAQIHGVVIRHWLQPHALERSEDGIV
EEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEE
HAITFEYTANRDGKLVGTGEYLTLEADQVFKAIGQTFDPEPLRGSGIALAKGRISVDAEG
EEEEEEEECCCCCCEEECCCEEEEEHHHHHHHHCCCCCCCCCCCCCEEEECCEEEECCCC
RTSVEGIWAGGDCVAGGKDLTVASVEDGKVAALSIHAALTSRPEAVEGFAEAVLSGAAHY
CCCCCEEEECCCEECCCCCEEEEEECCCCEEEEEEEEHHCCCCHHHHHHHHHHHHCCHHC
APAAPSRHATVQPGSEQGLGD
CCCCCCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]