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The map label for this gene is gyrB [H]

Identifier: 23501039

GI number: 23501039

Start: 140014

End: 142446

Strand: Reverse

Name: gyrB [H]

Synonym: BR0125

Alternate gene names: 23501039

Gene position: 142446-140014 (Counterclockwise)

Preceding gene: 23501040

Following gene: 23501035

Centisome position: 6.76

GC content: 59.76

Gene sequence:

>2433_bases
ATGCCCGAGATGAATTCCGAAGCCCCCGCCGAATATGGCGCGGATTCCATTCGTGTTCTGAAAGGTCTGGATGCTGTTCG
CAAGCGCCCCGGCATGTATATCGGCGACACGGACGACGGCTCCGGCCTGCATCACATGGTCTATGAAGTCGTGGACAACG
CCATCGACGAAGCACTGGCCGGACATGCCACGCTCGTCACCGTAACGCTCAATGCCGACGGCTCCTGCACCGTGACCGAC
AACGGGCGCGGCATCCCGACCGACATCCACAAGGAAGAAGGCGTTTCAGCTGCCGAGGTCATCATGACCCAGCTTCATGC
GGGCGGCAAATTCGACCAGAATTCCTACAAGGTTTCCGGCGGCCTGCATGGCGTCGGCGTTTCAGTGGTGAATGCGCTGT
CGATCTGGCTGAAGCTGAAAATCCGGCGCGCGGGCAAAATCCATGAAATGAGCTTCACACACGGCGTTGCCGATGCGCCC
TTGGTCGTAACCGGCGATGCAGGCAGTGAAACCGGCACGGAAGTGAGCTTCCTGCCCTCGCCTGACACCTTCAGCATGGT
CGAATTCGACTATGAAACGCTGGAACGCCGCCTGCGCGAACTTGCCTTCCTCAATTCGGGCGTGCGCATCAAGCTCGCCG
ACCGCCGCCATGCGGATGTGAAGGAACAGGAACTGCATTATGACGGCGGCCTGGTCGAATTCGTCAAATATATCGACCAG
AGCAAGAAGTCGCTTCTGGAAGAGCCTATCTATATTCGCAGCGAAAAGGATGGCATGACGGTGGAAGTCGCCATGTGGTG
GAACGATTCCTACCACGAGAAGGTGCTGTGCTTCACCAACAACATCCCGCAGCGCGACGGCGGCACGCATCTGGCCGGTT
TCCGCGGCGCGCTGACCCGACAGATCACGGGCTATGCGGACTCTTCCGGCATGACGAAGAAGGAAAAGGTACAGCTTACC
GGCGACGATTGCCGCGAGGGCCTGACCGCCGTTCTTTCCGTGAAGGTGCCGGATCCGAAATTCTCCTCGCAGACCAAGGA
CAAGCTTGTTTCCTCCGAAGTGCGCCCGGTGGTGGAAGGCCTCGTCAATGAAGCGCTTTCCACATGGCTTGAAGAACATC
CGGCAGGCGGCAAGATCGTTGTCGAGAAGGTCATTCAGGCGGCGGCGGCCCGCGAAGCCGCCCGCAAGGCGCGCGACATC
ACCCGCAAGAGCAATCTCAGCGTGACGTCCCTGCCCGGAAAGCTTGCCGATTGCCAGGAGCGCGATCCGGCCAAGTCCGA
AATCTTCATCGTCGAGGGCGATTCGGCAGGCGGTTCCGCCAAGAGCGGGCGCTCGCGCCAGAATCAGGCCATTCTGCCGC
TGCGCGGCAAAATCCTGAACGTGGAACGCGTGCGTTTCGACCGGATGATTTCATCCGATCAGGTGGGCACCCTCATCACG
GCGCTTGGCACCTCCATCGGCAAGGATGAAACGCACGGCTTCAACGCCGACAAACTGCGTTATCACAAGATCATCATCAT
GACCGACGCCGACGTCGATGGCGCCCATATTCGTACGCTTCTGCTCACCTTCTTCTTCCGGCAGATGCCGGAACTGATCG
AACGCGGGCATATCTATATCGCGCAGCCGCCGCTCTATAAGGTGACACGCGGCAAGTCTTCGCAATATATCAAGAACGAA
GCCGCCTTTGAGGATTTCCTCATCGAAACCGGCCTTGAAGAAACGACACTGGAACTGGTGACTGGCGAAATGCGCGCCGG
GCCGGATTTGCGCTCGGTGGTGGAGGATGCGCGCATGCTGCGTCAGCTTCTGCACGGCCTGCACACCCGCTATGACCGCA
GCGTGGTGGAACAGGCGGCAATTGCCGGCCTGCTCAACCCCGATGCCTCAAGGGACAATGCAACGGCACAGCATTCCGCC
GATACGGTTGCCAAGCGTCTCGACATGATTTCGGAAGAGACCGAGCGCGGCTGGAGCGGCCATGTGATGGAAGACGGCGG
CTATCGCTTCGAGCGTATGGTGCGCGGTGTAAAGGATATCGCCATTCTCGACATGGCCCTGCTCGGCTCGGCCGATGCCC
GCCAGGTCGACCGCGTTGCGGGCCGCATGGCTGAAATCTTCATGGAGCCGCCGGTGCTGCGCCGCAAGGACAAGGTGGAA
TCGCTTTCCGGCCCGGTGGCCCTGCTTGACGCCGTGTTTGCAACCGGCCGCAAGGGTCTGACCCTCCAGCGTTACAAAGG
CTTGGGCGAAATGAACGCCGAACAGCTTTGGGAAACGACACTTGATCCGAATGTGCGTTCGCTCCTGCAGGTGAAGGTGA
ACGATGCAACCGATGCCGATTCCCTCTTCTCGCGCCTTATGGGCGACGAGGTGGAGCCGCGGCGTGAATTCATTCAGGAT
AATGCGCTAAACGTCGCCAATCTGGACGTTTGA

Upstream 100 bases:

>100_bases
TCGCTGTTAGCACCTATATTAAAGCCATGTTTGTCGCGTGATTCGACATATCTTTGGAGCTGCATTCGCAGCAGTTTTTT
GAAAGATTTTCATGAGCGAG

Downstream 100 bases:

>100_bases
TACAAAAAGCCCCGGAAATCCGGGGCTTTTTTGCACTTTTGACTTGAAATTGCTCCAGCCAAAACCCGGTGAGCGAAGCT
CATCGGGTTCTGCTATTATT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MPEMNSEAPAEYGADSIRVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHATLVTVTLNADGSCTVTD
NGRGIPTDIHKEEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSIWLKLKIRRAGKIHEMSFTHGVADAP
LVVTGDAGSETGTEVSFLPSPDTFSMVEFDYETLERRLRELAFLNSGVRIKLADRRHADVKEQELHYDGGLVEFVKYIDQ
SKKSLLEEPIYIRSEKDGMTVEVAMWWNDSYHEKVLCFTNNIPQRDGGTHLAGFRGALTRQITGYADSSGMTKKEKVQLT
GDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVEGLVNEALSTWLEEHPAGGKIVVEKVIQAAAAREAARKARDI
TRKSNLSVTSLPGKLADCQERDPAKSEIFIVEGDSAGGSAKSGRSRQNQAILPLRGKILNVERVRFDRMISSDQVGTLIT
ALGTSIGKDETHGFNADKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELIERGHIYIAQPPLYKVTRGKSSQYIKNE
AAFEDFLIETGLEETTLELVTGEMRAGPDLRSVVEDARMLRQLLHGLHTRYDRSVVEQAAIAGLLNPDASRDNATAQHSA
DTVAKRLDMISEETERGWSGHVMEDGGYRFERMVRGVKDIAILDMALLGSADARQVDRVAGRMAEIFMEPPVLRRKDKVE
SLSGPVALLDAVFATGRKGLTLQRYKGLGEMNAEQLWETTLDPNVRSLLQVKVNDATDADSLFSRLMGDEVEPRREFIQD
NALNVANLDV

Sequences:

>Translated_810_residues
MPEMNSEAPAEYGADSIRVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHATLVTVTLNADGSCTVTD
NGRGIPTDIHKEEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSIWLKLKIRRAGKIHEMSFTHGVADAP
LVVTGDAGSETGTEVSFLPSPDTFSMVEFDYETLERRLRELAFLNSGVRIKLADRRHADVKEQELHYDGGLVEFVKYIDQ
SKKSLLEEPIYIRSEKDGMTVEVAMWWNDSYHEKVLCFTNNIPQRDGGTHLAGFRGALTRQITGYADSSGMTKKEKVQLT
GDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVEGLVNEALSTWLEEHPAGGKIVVEKVIQAAAAREAARKARDI
TRKSNLSVTSLPGKLADCQERDPAKSEIFIVEGDSAGGSAKSGRSRQNQAILPLRGKILNVERVRFDRMISSDQVGTLIT
ALGTSIGKDETHGFNADKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELIERGHIYIAQPPLYKVTRGKSSQYIKNE
AAFEDFLIETGLEETTLELVTGEMRAGPDLRSVVEDARMLRQLLHGLHTRYDRSVVEQAAIAGLLNPDASRDNATAQHSA
DTVAKRLDMISEETERGWSGHVMEDGGYRFERMVRGVKDIAILDMALLGSADARQVDRVAGRMAEIFMEPPVLRRKDKVE
SLSGPVALLDAVFATGRKGLTLQRYKGLGEMNAEQLWETTLDPNVRSLLQVKVNDATDADSLFSRLMGDEVEPRREFIQD
NALNVANLDV
>Mature_809_residues
PEMNSEAPAEYGADSIRVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGHATLVTVTLNADGSCTVTDN
GRGIPTDIHKEEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSIWLKLKIRRAGKIHEMSFTHGVADAPL
VVTGDAGSETGTEVSFLPSPDTFSMVEFDYETLERRLRELAFLNSGVRIKLADRRHADVKEQELHYDGGLVEFVKYIDQS
KKSLLEEPIYIRSEKDGMTVEVAMWWNDSYHEKVLCFTNNIPQRDGGTHLAGFRGALTRQITGYADSSGMTKKEKVQLTG
DDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVEGLVNEALSTWLEEHPAGGKIVVEKVIQAAAAREAARKARDIT
RKSNLSVTSLPGKLADCQERDPAKSEIFIVEGDSAGGSAKSGRSRQNQAILPLRGKILNVERVRFDRMISSDQVGTLITA
LGTSIGKDETHGFNADKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELIERGHIYIAQPPLYKVTRGKSSQYIKNEA
AFEDFLIETGLEETTLELVTGEMRAGPDLRSVVEDARMLRQLLHGLHTRYDRSVVEQAAIAGLLNPDASRDNATAQHSAD
TVAKRLDMISEETERGWSGHVMEDGGYRFERMVRGVKDIAILDMALLGSADARQVDRVAGRMAEIFMEPPVLRRKDKVES
LSGPVALLDAVFATGRKGLTLQRYKGLGEMNAEQLWETTLDPNVRSLLQVKVNDATDADSLFSRLMGDEVEPRREFIQDN
ALNVANLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=560, Percent_Identity=23.3928571428571, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI19913408, Length=550, Percent_Identity=24, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI48994957, Length=807, Percent_Identity=56.5055762081784, Blast_Score=865, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=558, Percent_Identity=40.5017921146953, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17535065, Length=537, Percent_Identity=24.3947858472998, Blast_Score=132, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=509, Percent_Identity=24.3614931237721, Blast_Score=117, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=139, Percent_Identity=33.0935251798561, Blast_Score=76, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=609, Percent_Identity=24.4663382594417, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI17136538, Length=553, Percent_Identity=25.497287522604, Blast_Score=142, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89172; Mature: 89041

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEMNSEAPAEYGADSIRVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALA
CCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHC
GHATLVTVTLNADGSCTVTDNGRGIPTDIHKEEGVSAAEVIMTQLHAGGKFDQNSYKVSG
CCEEEEEEEECCCCCEEECCCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCCCEEECC
GLHGVGVSVVNALSIWLKLKIRRAGKIHEMSFTHGVADAPLVVTGDAGSETGTEVSFLPS
CCCHHHHHHHHHHHHHHHEEEHHCCCEEEEHHHCCCCCCCEEEECCCCCCCCCEEEECCC
PDTFSMVEFDYETLERRLRELAFLNSGVRIKLADRRHADVKEQELHYDGGLVEFVKYIDQ
CCCCEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHCCCCCHHHHHHHHHH
SKKSLLEEPIYIRSEKDGMTVEVAMWWNDSYHEKVLCFTNNIPQRDGGTHLAGFRGALTR
HHHHHHCCCCEEEECCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHH
QITGYADSSGMTKKEKVQLTGDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVEG
HHCCCCCCCCCCCHHEEEECCHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
LVNEALSTWLEEHPAGGKIVVEKVIQAAAAREAARKARDITRKSNLSVTSLPGKLADCQE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHCCCC
RDPAKSEIFIVEGDSAGGSAKSGRSRQNQAILPLRGKILNVERVRFDRMISSDQVGTLIT
CCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCEECHHHHHHHHHHCCCHHHHHHH
ALGTSIGKDETHGFNADKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELIERGHIYI
HHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
AQPPLYKVTRGKSSQYIKNEAAFEDFLIETGLEETTLELVTGEMRAGPDLRSVVEDARML
ECCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHCCCCHHHHHHHHHHHH
RQLLHGLHTRYDRSVVEQAAIAGLLNPDASRDNATAQHSADTVAKRLDMISEETERGWSG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
HVMEDGGYRFERMVRGVKDIAILDMALLGSADARQVDRVAGRMAEIFMEPPVLRRKDKVE
CEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
SLSGPVALLDAVFATGRKGLTLQRYKGLGEMNAEQLWETTLDPNVRSLLQVKVNDATDAD
HHCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHCCCCCHHHHEEEEECCCCCHH
SLFSRLMGDEVEPRREFIQDNALNVANLDV
HHHHHHHCCCCCHHHHHHHCCCCCEECCCC
>Mature Secondary Structure 
PEMNSEAPAEYGADSIRVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALA
CCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHC
GHATLVTVTLNADGSCTVTDNGRGIPTDIHKEEGVSAAEVIMTQLHAGGKFDQNSYKVSG
CCEEEEEEEECCCCCEEECCCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCCCEEECC
GLHGVGVSVVNALSIWLKLKIRRAGKIHEMSFTHGVADAPLVVTGDAGSETGTEVSFLPS
CCCHHHHHHHHHHHHHHHEEEHHCCCEEEEHHHCCCCCCCEEEECCCCCCCCCEEEECCC
PDTFSMVEFDYETLERRLRELAFLNSGVRIKLADRRHADVKEQELHYDGGLVEFVKYIDQ
CCCCEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHCCCCCHHHHHHHHHH
SKKSLLEEPIYIRSEKDGMTVEVAMWWNDSYHEKVLCFTNNIPQRDGGTHLAGFRGALTR
HHHHHHCCCCEEEECCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHH
QITGYADSSGMTKKEKVQLTGDDCREGLTAVLSVKVPDPKFSSQTKDKLVSSEVRPVVEG
HHCCCCCCCCCCCHHEEEECCHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHH
LVNEALSTWLEEHPAGGKIVVEKVIQAAAAREAARKARDITRKSNLSVTSLPGKLADCQE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHCCCC
RDPAKSEIFIVEGDSAGGSAKSGRSRQNQAILPLRGKILNVERVRFDRMISSDQVGTLIT
CCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCCCCCEECHHHHHHHHHHCCCHHHHHHH
ALGTSIGKDETHGFNADKLRYHKIIIMTDADVDGAHIRTLLLTFFFRQMPELIERGHIYI
HHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
AQPPLYKVTRGKSSQYIKNEAAFEDFLIETGLEETTLELVTGEMRAGPDLRSVVEDARML
ECCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHCCCCHHHHHHHHHHHH
RQLLHGLHTRYDRSVVEQAAIAGLLNPDASRDNATAQHSADTVAKRLDMISEETERGWSG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
HVMEDGGYRFERMVRGVKDIAILDMALLGSADARQVDRVAGRMAEIFMEPPVLRRKDKVE
CEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHH
SLSGPVALLDAVFATGRKGLTLQRYKGLGEMNAEQLWETTLDPNVRSLLQVKVNDATDAD
HHCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHCCCCCHHHHEEEEECCCCCHH
SLFSRLMGDEVEPRREFIQDNALNVANLDV
HHHHHHHCCCCCHHHHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226640 [H]