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The map label for this gene is dapE [H]

Identifier: 23501035

GI number: 23501035

Start: 132981

End: 134396

Strand: Reverse

Name: dapE [H]

Synonym: BR0121

Alternate gene names: 23501035

Gene position: 134396-132981 (Counterclockwise)

Preceding gene: 23501039

Following gene: 23501034

Centisome position: 6.38

GC content: 59.6

Gene sequence:

>1416_bases
ATGTCCACGCTATCACTCGACAAAGTTCTCAACCATCTCGACGCCAATCTCAACAAAAGCCTCGACCGCCTGTTCAATCT
TTTGCGGATCAAGTCGATTTCGACCGACCCGGCCTACAAGGCCGATTGCCGCAAGGCGGCCGAGTGGCTGGTGGAAGACC
TGAAGTCTATCGGTTTTGATGCCAGCGTGCGCGACACACCCGGCCACCCGATGGTTGTTGCCCACCACGAAGGGGCGACA
GCCGATGCGCCGCATGTGCTTTTCTATGGCCATTACGACGTTCAGCCTGTCGATCCGCTCAGCCTGTGGGAAAACGATCC
CTTCGATCCGGCTATCAAGGATGTCGGCGATGCCTCGAATGGGGGCCGCAAGATCCTGACCGGACGCGGCACCTCCGACG
ACAAGGGGCAGCTCATGACTTTTGTGGAAGCCTGCCGCGCCTATAAGGCGGTGAACGGCAGCCTGCCGGTCAAGGTGACG
CTCCTGTTTGAAGGTGAAGAGGAATCCGGCTCACCTTCGCTGAAGCCTTTTCTTGAAGCCAACAGGCAAGAGCTGAAGGC
AGATGTGGCGCTCGTCTGCGATACCGCCATGTGGGACGCGGAAACGCCAGCGATCAGCGTGGGGCTGCGCGGCCTCGTCG
GCGAGGAAATCGTCATCAAGGCAGCCGACCGCGATCTGCATTCCGGCTTCTTCGGCGGTGCGGCGGCAAACCCCATCCAC
ATCCTGACCAAGATTCTGGCCGACCTGCACGATGAAACCGGGCGCATCACCATACCGGATTTCTATGAAGGCGTGGAGGA
AACCCCGACCCAGATTCTGAAATCCTGGGAAAGCCTTGGCCGTACGGCGGAAAGCTTCCTCGGCCCCATCGGCCTTTCCA
TTCCAGCGGGCGAAAAGGGCCGCAGCGTGCTGGAACTCACCTGGGCACGCCCGACTGCGGAAGTAAACGGCATTATCGGC
GGCTATACCGGCGAAGGCTTCAAGACCGTTATCGCAGCGGAAGCTTCCGCCAAGGTTTCGTTCCGCCTCGTGCACAAACA
GGATCCCGTCAAGATTCGCGAAGCCTTCCGCGCCTTCGTGAAAGAGCGTGTTCCCGCCGATTGCTCGGTCGAATTCCATC
CGCATGGCGGCTCGCCCGCAATCCAGCTGCCTTATGATTCCCCGCTCGTCTCCAAGGCGAAGAACGCCCTTTCCGACGAA
TGGCCGAAGCCTGCCGTGCTGATCGCCATGGGCGGCTCGATCCCGATCGTCGGCGACTTCAATACGTTCCTTGGCATGGA
ATCGCTTCTGGTCGGCTTCGGCCTTGAGGATGATCGCATCCATTCACCCAATGAAAAATACGAGCTGAACTCCTTCCATA
AGGGCCAGCGCTCCTGGGCTCGTATTTTGGCGGCTATCGCCGCCAAGCAAGGCTGA

Upstream 100 bases:

>100_bases
TCGCCTCTGAAATCCGCGTCAAGTCGAGCTTAATGAGGGTTTTAACTTCGCAAAGCTGGTTGTAGTGTGCCATCAGTTTT
ATGACTCGTCAGGAAGCCCC

Downstream 100 bases:

>100_bases
TATCATGGCGGCAATTGCCGCCAAATAAGGACAATTGAATGACTATCCGTTTTCATAGGAACGACCTGCCGAACCTCGAC
AATTATCAGGTGGATGCGGT

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSTLSLDKVLNHLDANLNKSLDRLFNLLRIKSISTDPAYKADCRKAAEWLVEDLKSIGFDASVRDTPGHPMVVAHHEGAT
ADAPHVLFYGHYDVQPVDPLSLWENDPFDPAIKDVGDASNGGRKILTGRGTSDDKGQLMTFVEACRAYKAVNGSLPVKVT
LLFEGEEESGSPSLKPFLEANRQELKADVALVCDTAMWDAETPAISVGLRGLVGEEIVIKAADRDLHSGFFGGAAANPIH
ILTKILADLHDETGRITIPDFYEGVEETPTQILKSWESLGRTAESFLGPIGLSIPAGEKGRSVLELTWARPTAEVNGIIG
GYTGEGFKTVIAAEASAKVSFRLVHKQDPVKIREAFRAFVKERVPADCSVEFHPHGGSPAIQLPYDSPLVSKAKNALSDE
WPKPAVLIAMGGSIPIVGDFNTFLGMESLLVGFGLEDDRIHSPNEKYELNSFHKGQRSWARILAAIAAKQG

Sequences:

>Translated_471_residues
MSTLSLDKVLNHLDANLNKSLDRLFNLLRIKSISTDPAYKADCRKAAEWLVEDLKSIGFDASVRDTPGHPMVVAHHEGAT
ADAPHVLFYGHYDVQPVDPLSLWENDPFDPAIKDVGDASNGGRKILTGRGTSDDKGQLMTFVEACRAYKAVNGSLPVKVT
LLFEGEEESGSPSLKPFLEANRQELKADVALVCDTAMWDAETPAISVGLRGLVGEEIVIKAADRDLHSGFFGGAAANPIH
ILTKILADLHDETGRITIPDFYEGVEETPTQILKSWESLGRTAESFLGPIGLSIPAGEKGRSVLELTWARPTAEVNGIIG
GYTGEGFKTVIAAEASAKVSFRLVHKQDPVKIREAFRAFVKERVPADCSVEFHPHGGSPAIQLPYDSPLVSKAKNALSDE
WPKPAVLIAMGGSIPIVGDFNTFLGMESLLVGFGLEDDRIHSPNEKYELNSFHKGQRSWARILAAIAAKQG
>Mature_470_residues
STLSLDKVLNHLDANLNKSLDRLFNLLRIKSISTDPAYKADCRKAAEWLVEDLKSIGFDASVRDTPGHPMVVAHHEGATA
DAPHVLFYGHYDVQPVDPLSLWENDPFDPAIKDVGDASNGGRKILTGRGTSDDKGQLMTFVEACRAYKAVNGSLPVKVTL
LFEGEEESGSPSLKPFLEANRQELKADVALVCDTAMWDAETPAISVGLRGLVGEEIVIKAADRDLHSGFFGGAAANPIHI
LTKILADLHDETGRITIPDFYEGVEETPTQILKSWESLGRTAESFLGPIGLSIPAGEKGRSVLELTWARPTAEVNGIIGG
YTGEGFKTVIAAEASAKVSFRLVHKQDPVKIREAFRAFVKERVPADCSVEFHPHGGSPAIQLPYDSPLVSKAKNALSDEW
PKPAVLIAMGGSIPIVGDFNTFLGMESLLVGFGLEDDRIHSPNEKYELNSFHKGQRSWARILAAIAAKQG

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=480, Percent_Identity=27.9166666666667, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI21071039, Length=480, Percent_Identity=26.0416666666667, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI271398277, Length=320, Percent_Identity=29.375, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17563146, Length=471, Percent_Identity=29.2993630573248, Blast_Score=187, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI193211059, Length=482, Percent_Identity=27.1784232365145, Blast_Score=171, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6321159, Length=482, Percent_Identity=29.253112033195, Blast_Score=187, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6319758, Length=459, Percent_Identity=24.8366013071895, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24585880, Length=470, Percent_Identity=29.1489361702128, Blast_Score=182, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 51025; Mature: 50894

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLSLDKVLNHLDANLNKSLDRLFNLLRIKSISTDPAYKADCRKAAEWLVEDLKSIGFD
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
ASVRDTPGHPMVVAHHEGATADAPHVLFYGHYDVQPVDPLSLWENDPFDPAIKDVGDASN
CCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCCHHHHHCCCCCC
GGRKILTGRGTSDDKGQLMTFVEACRAYKAVNGSLPVKVTLLFEGEEESGSPSLKPFLEA
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCHHHHC
NRQELKADVALVCDTAMWDAETPAISVGLRGLVGEEIVIKAADRDLHSGFFGGAAANPIH
CHHHHHHHHHEEECCHHCCCCCCEEECCHHCCCCCEEEEEECCCHHHCCCCCCCCCCHHH
ILTKILADLHDETGRITIPDFYEGVEETPTQILKSWESLGRTAESFLGPIGLSIPAGEKG
HHHHHHHHHCCCCCEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
RSVLELTWARPTAEVNGIIGGYTGEGFKTVIAAEASAKVSFRLVHKQDPVKIREAFRAFV
CEEEEEEECCCCCHHCCEEECCCCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHH
KERVPADCSVEFHPHGGSPAIQLPYDSPLVSKAKNALSDEWPKPAVLIAMGGSIPIVGDF
HHCCCCCCEEEECCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECC
NTFLGMESLLVGFGLEDDRIHSPNEKYELNSFHKGQRSWARILAAIAAKQG
HHHHHHHHHHHHCCCCCCCCCCCCCCEECCHHHCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STLSLDKVLNHLDANLNKSLDRLFNLLRIKSISTDPAYKADCRKAAEWLVEDLKSIGFD
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
ASVRDTPGHPMVVAHHEGATADAPHVLFYGHYDVQPVDPLSLWENDPFDPAIKDVGDASN
CCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCCHHHHHCCCCCC
GGRKILTGRGTSDDKGQLMTFVEACRAYKAVNGSLPVKVTLLFEGEEESGSPSLKPFLEA
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCHHHHC
NRQELKADVALVCDTAMWDAETPAISVGLRGLVGEEIVIKAADRDLHSGFFGGAAANPIH
CHHHHHHHHHEEECCHHCCCCCCEEECCHHCCCCCEEEEEECCCHHHCCCCCCCCCCHHH
ILTKILADLHDETGRITIPDFYEGVEETPTQILKSWESLGRTAESFLGPIGLSIPAGEKG
HHHHHHHHHCCCCCEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
RSVLELTWARPTAEVNGIIGGYTGEGFKTVIAAEASAKVSFRLVHKQDPVKIREAFRAFV
CEEEEEEECCCCCHHCCEEECCCCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHH
KERVPADCSVEFHPHGGSPAIQLPYDSPLVSKAKNALSDEWPKPAVLIAMGGSIPIVGDF
HHCCCCCCEEEECCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECC
NTFLGMESLLVGFGLEDDRIHSPNEKYELNSFHKGQRSWARILAAIAAKQG
HHHHHHHHHHHHCCCCCCCCCCCCCCEECCHHHCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA