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The map label for this gene is trmE

Identifier: 23100946

GI number: 23100946

Start: 3625670

End: 3627049

Strand: Reverse

Name: trmE

Synonym: OB3491

Alternate gene names: 23100946

Gene position: 3627049-3625670 (Counterclockwise)

Preceding gene: 23100947

Following gene: 23100945

Centisome position: 99.9

GC content: 37.39

Gene sequence:

>1380_bases
ATGTTGGATACAGATACAATTACTGCGATATCGACTCCTGTTGGGGAGGGTGCTATTGCAATCGTTCGTTTAAGTGGGTC
AGAAGCAATTACCATTACTTCACAAATATTTGAAGGTAAGAATCTACAAGAGGTCCCTTCACATACGATCCAATATGGAA
AGATAATTGACCCTGGTACTAATGAGGTTGCAGAAGAAGTCATGGTTTCTATTATGCGTGGGCCAAAAACATTTACACGT
GAAGATGTAGTAGAAATTAATTGCCATGGTGGTATGGTTGCTGTGAATCGTGTATTGGAAATTGTTTTAGCAAAAGGTGT
TCGTTTAGCGGAACCTGGTGAATTTACGAAGCGTGCATTTTTACACGGAAGAATTGATTTATCGCAAGCAGAAGCAGTGA
TGGATTTAATTCGTGCCAAAACAGATAAGGCAATGTCTGTTGCATTAAAACAAATGGATGGACGATTATCGAAATTAATT
CAGAAGTTACGCCAAGAATTGTTAGAAACAGTTGCTCATGTAGAAGTAAATATTGATTATCCCGAATATGATGATGTGGA
AGAAATGTCACATGCAATGATGAAAGAAAAATCAAAGGAAGTGCGGGATGAATTAGATAAACTACTGCAAATAGCACAAC
AAGGAAAGATTTTACGTGAAGGATTGTCAACTGCTATTATTGGACGTCCAAACGTAGGAAAATCCTCGTTAATGAATACG
CTTGTGCAGGAAAATAAAGCAATCGTAACAGAAATACCAGGAACAACACGTGATATAATCGAAGAATATGTTAATGTGCG
CGGTGTTCCACTACGTTTAGTAGATACGGCAGGAATAAGAGAGACCGAGGATATCGTAGAGAGAATCGGTGTCGATCGTT
CGAGACAAGTATTAAAAGAATCGGATTTAATTTTATTTGTGCTTAATTATAATGAACCACTATCGGAGGACGATAAGAAA
TTATTTGAAGCGGTAGATGGATTAGAATATATTGTGATTATTAATAAAACAGATTTAGAGCAACAATTGGATTTAGATGA
AGTACGTGAATTTGCAAAAGATAGACCAGTTGTTACTACTGCCTTATTAGAAGAACAAGGGGTAGATGAGCTTGAAAAAG
CCATTGCCGATACGTTCTTTACCGGAGATATAGATACTGGCGATATGACGTATGTTTCGAATGTCCGTCATATACAGCTA
CTAAAACAAGCGAAACAAGCTTTGGAAGACGCGATGGAAGGCATTGAATTAGGGATGCCGATGGATATCGTTCAAATTGA
TGTGACAAGATCATGGGAATTCATGGGTGAAATCATTGGTGATACTGCTAGTGATAGTCTGATTGATCAGTTATTCTCGC
AATTTTGTTTAGGAAAATAA

Upstream 100 bases:

>100_bases
TGATACGTTTGCCGATTTTTCCCATGTAGATAGTGAGAATGGTTTGTATGGACTGCTATTCATCGTAATATGCAGTCTAC
AATGTGTGGAGGTGACAAAA

Downstream 100 bases:

>100_bases
GATAGGATAATTTTTGTAGAAAGGAAGAGAAAACATGACCTATAATGCAGGTAATTATGATGTCATCGTTGTTGGTGCTG
GCCATGCGGGAGTAGAGTCA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MLDTDTITAISTPVGEGAIAIVRLSGSEAITITSQIFEGKNLQEVPSHTIQYGKIIDPGTNEVAEEVMVSIMRGPKTFTR
EDVVEINCHGGMVAVNRVLEIVLAKGVRLAEPGEFTKRAFLHGRIDLSQAEAVMDLIRAKTDKAMSVALKQMDGRLSKLI
QKLRQELLETVAHVEVNIDYPEYDDVEEMSHAMMKEKSKEVRDELDKLLQIAQQGKILREGLSTAIIGRPNVGKSSLMNT
LVQENKAIVTEIPGTTRDIIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVDRSRQVLKESDLILFVLNYNEPLSEDDKK
LFEAVDGLEYIVIINKTDLEQQLDLDEVREFAKDRPVVTTALLEEQGVDELEKAIADTFFTGDIDTGDMTYVSNVRHIQL
LKQAKQALEDAMEGIELGMPMDIVQIDVTRSWEFMGEIIGDTASDSLIDQLFSQFCLGK

Sequences:

>Translated_459_residues
MLDTDTITAISTPVGEGAIAIVRLSGSEAITITSQIFEGKNLQEVPSHTIQYGKIIDPGTNEVAEEVMVSIMRGPKTFTR
EDVVEINCHGGMVAVNRVLEIVLAKGVRLAEPGEFTKRAFLHGRIDLSQAEAVMDLIRAKTDKAMSVALKQMDGRLSKLI
QKLRQELLETVAHVEVNIDYPEYDDVEEMSHAMMKEKSKEVRDELDKLLQIAQQGKILREGLSTAIIGRPNVGKSSLMNT
LVQENKAIVTEIPGTTRDIIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVDRSRQVLKESDLILFVLNYNEPLSEDDKK
LFEAVDGLEYIVIINKTDLEQQLDLDEVREFAKDRPVVTTALLEEQGVDELEKAIADTFFTGDIDTGDMTYVSNVRHIQL
LKQAKQALEDAMEGIELGMPMDIVQIDVTRSWEFMGEIIGDTASDSLIDQLFSQFCLGK
>Mature_459_residues
MLDTDTITAISTPVGEGAIAIVRLSGSEAITITSQIFEGKNLQEVPSHTIQYGKIIDPGTNEVAEEVMVSIMRGPKTFTR
EDVVEINCHGGMVAVNRVLEIVLAKGVRLAEPGEFTKRAFLHGRIDLSQAEAVMDLIRAKTDKAMSVALKQMDGRLSKLI
QKLRQELLETVAHVEVNIDYPEYDDVEEMSHAMMKEKSKEVRDELDKLLQIAQQGKILREGLSTAIIGRPNVGKSSLMNT
LVQENKAIVTEIPGTTRDIIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVDRSRQVLKESDLILFVLNYNEPLSEDDKK
LFEAVDGLEYIVIINKTDLEQQLDLDEVREFAKDRPVVTTALLEEQGVDELEKAIADTFFTGDIDTGDMTYVSNVRHIQL
LKQAKQALEDAMEGIELGMPMDIVQIDVTRSWEFMGEIIGDTASDSLIDQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=471, Percent_Identity=31.2101910828025, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI193082993, Length=460, Percent_Identity=30.8695652173913, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI193082988, Length=500, Percent_Identity=29.6, Blast_Score=201, Evalue=9e-52,
Organism=Escherichia coli, GI2367268, Length=471, Percent_Identity=33.5456475583864, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI87082120, Length=131, Percent_Identity=34.3511450381679, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=464, Percent_Identity=29.5258620689655, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323665, Length=495, Percent_Identity=28.2828282828283, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45550813, Length=488, Percent_Identity=31.5573770491803, Blast_Score=205, Evalue=7e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_OCEIH (Q8CX54)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_694413.1
- ProteinModelPortal:   Q8CX54
- SMR:   Q8CX54
- GeneID:   1016170
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3491
- NMPDR:   fig|221109.1.peg.3495
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   OIHE221109:OB3491-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51177; Mature: 51177

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 85-85 BINDING 124-124 BINDING 459-459

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDTDTITAISTPVGEGAIAIVRLSGSEAITITSQIFEGKNLQEVPSHTIQYGKIIDPGT
CCCCCCCCEECCCCCCCCEEEEEECCCCEEEEEHHHHCCCCHHHHHHHHHHCCCCCCCCC
NEVAEEVMVSIMRGPKTFTREDVVEINCHGGMVAVNRVLEIVLAKGVRLAEPGEFTKRAF
HHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LHGRIDLSQAEAVMDLIRAKTDKAMSVALKQMDGRLSKLIQKLRQELLETVAHVEVNIDY
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
PEYDDVEEMSHAMMKEKSKEVRDELDKLLQIAQQGKILREGLSTAIIGRPNVGKSSLMNT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCEEECCCCCCCHHHHHHH
LVQENKAIVTEIPGTTRDIIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVDRSRQVLKE
HHHCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHCC
SDLILFVLNYNEPLSEDDKKLFEAVDGLEYIVIINKTDLEQQLDLDEVREFAKDRPVVTT
CCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHCCHHHHHHHHCCCCCHHH
ALLEEQGVDELEKAIADTFFTGDIDTGDMTYVSNVRHIQLLKQAKQALEDAMEGIELGMP
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MDIVQIDVTRSWEFMGEIIGDTASDSLIDQLFSQFCLGK
CEEEEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDTDTITAISTPVGEGAIAIVRLSGSEAITITSQIFEGKNLQEVPSHTIQYGKIIDPGT
CCCCCCCCEECCCCCCCCEEEEEECCCCEEEEEHHHHCCCCHHHHHHHHHHCCCCCCCCC
NEVAEEVMVSIMRGPKTFTREDVVEINCHGGMVAVNRVLEIVLAKGVRLAEPGEFTKRAF
HHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LHGRIDLSQAEAVMDLIRAKTDKAMSVALKQMDGRLSKLIQKLRQELLETVAHVEVNIDY
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
PEYDDVEEMSHAMMKEKSKEVRDELDKLLQIAQQGKILREGLSTAIIGRPNVGKSSLMNT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCEEECCCCCCCHHHHHHH
LVQENKAIVTEIPGTTRDIIEEYVNVRGVPLRLVDTAGIRETEDIVERIGVDRSRQVLKE
HHHCCCEEEEECCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCHHHHHHCC
SDLILFVLNYNEPLSEDDKKLFEAVDGLEYIVIINKTDLEQQLDLDEVREFAKDRPVVTT
CCEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHCCHHHHHHHHCCCCCHHH
ALLEEQGVDELEKAIADTFFTGDIDTGDMTYVSNVRHIQLLKQAKQALEDAMEGIELGMP
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MDIVQIDVTRSWEFMGEIIGDTASDSLIDQLFSQFCLGK
CEEEEEEECHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376