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The map label for this gene is gidA

Identifier: 23100945

GI number: 23100945

Start: 3623746

End: 3625635

Strand: Reverse

Name: gidA

Synonym: OB3490

Alternate gene names: 23100945

Gene position: 3625635-3623746 (Counterclockwise)

Preceding gene: 23100946

Following gene: 23100944

Centisome position: 99.87

GC content: 38.15

Gene sequence:

>1890_bases
ATGACCTATAATGCAGGTAATTATGATGTCATCGTTGTTGGTGCTGGCCATGCGGGAGTAGAGTCAGCATATGCTGCTGC
TCGTATGGGAGCAAAAACGTTAATGCTGACACTTAATTTAGATATGATTGCTTTTATGCCATGTAATCCATCTATTGGTG
GTCCTGCCAAAGGAATCGTCGTTCGTGAAATTGACGCATTAGGTGGGATTATGGGGAAAGTAATTGATAAAAGTTATATT
CAAATGCGTATGTTGAATACAGGTAAAGGTCCAGCAGTGCAAGCATTGCGTGCACAAGCGGATAAGCCGATATATATGAA
AGAAATGAAGCATTTAATGGAAAACGAGGAAAACCTTACGATACGTCAAGGTATGGTAAATGAATTAATCGTGGAGGATG
GGATTTGTAAAGGTGTTATTACAGAAACCCATGCAGCCTATTATGCAGACTCCGTTATTGTAACGACTGGTACATTTATG
CGAGGGAAAGTGTTAATGGGGGACTTGGAATATGAGAGTGGTCCTAATAACCAACGTGTCTCAATTAAATTGTCTGAGGA
TCTTGAAAAGCACGGATTTGAAATAACTCGTTTTAAAACAGGTACACCACCAAGAGTTAATAGTCACACTATCGATTATT
CAAAAACAGAGATACAGCCAGGAGATGACAATCCGAAATCATTTTCTTATGAGACTACAGAGCAAATAACCGATCAAATC
CCTTGTTGGTTAACGTATACAAATGAATTTACCCATCAAGTGATTAATGACAATTTAGAGTTATCTTCCATGTATTCTGG
GATGAAGCGTGGAACAGGTCCTCGTTATTGTCCTTCTATTGAAGATAAGATTGTGCGTTTTAATGATAAACCACGTCATC
AAATCTTCTTAGAACCTGAAGGAAGAGATACAGAGGAAGTGTATGTTCAAGGTCTATCTACTTCATTACCAGAAAGTATC
CAGCATGAAATGATTAAATCGGTACCTGGGTTGGAGAAAGCAGAAATTATGCGTGCCGGTTATGCCATCGAGTATGATGC
AGTAGTACCAACGCAACTATGGCCGACCTTAGAAACAAAAAGATTGCCAGGATTATTTACGGCAGGTCAAATCAATGGTA
CTTCGGGATACGAAGAAGCAGCTGGTCAAGGATTAATGGCAGGAATGAACGCGGCAGCGAAGTCATTAAATAAAGAACCG
ATTATATTGGATCGTTCACAAGCGTATATCGGTGTTATGATTGATGATTTAGTTACAAAAGGAACAAATGAGCCGTATCG
TCTGCTAACTTCTCGAGCAGAATATCGTCTATTATTACGCCATGATAATGCTGATTTGCGACTAACAGATATTGGTTATG
ATTACGGACTTATTACCGAGGAACGTTATCAACGATTTACGGAGAAAAAACAGCAAATTGAACAAGAAAAGAAGCGATTA
AGTAAGATTATCATTAAACCAACGGAAGATATTCAAGATATTATGGAAAAAGCAGGAGCGTCCCGCTTAAAAGAAGCAAT
AAAAGCGTATGATTTAATGAAGCGTCCAGAGCTATCTTATGATATCGTCGATCAAATGATCGAAAGTAATTTGGAACTCT
CAGAAGAAGTGAAAGAACAGGTTGCTATTCAAATCAAATATGAAGGGTATATTAAAAAAGCAAAAGAACAAGTTGATCGA
ATGCTTAAAATGGAAGATAAGAAAATACCTGAGGATATTGATTATGATGCAATTAACGGAATAGCGACAGAAGCAGTGGA
AAAACTCAAAAAAGTACGTCCACTCTCTGTTGGGCAAGCATCACGTATATCTGGAGTAAATCCCGCTGATGTATCCATAT
TACTTGTATATATTGAACAAGGAAGTATTGCCAAAGTAGCGAATGATTGA

Upstream 100 bases:

>100_bases
ATTGGTGATACTGCTAGTGATAGTCTGATTGATCAGTTATTCTCGCAATTTTGTTTAGGAAAATAAGATAGGATAATTTT
TGTAGAAAGGAAGAGAAAAC

Downstream 100 bases:

>100_bases
TTATATAGCGGCGCGTCACCAGTATGCGTCGCTTTTTCCTTATAAAAGTAGTGTTAAGGGAGGATCTCATGAAACCAGAA
CAATTTGTACACGCATTAAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MTYNAGNYDVIVVGAGHAGVESAYAAARMGAKTLMLTLNLDMIAFMPCNPSIGGPAKGIVVREIDALGGIMGKVIDKSYI
QMRMLNTGKGPAVQALRAQADKPIYMKEMKHLMENEENLTIRQGMVNELIVEDGICKGVITETHAAYYADSVIVTTGTFM
RGKVLMGDLEYESGPNNQRVSIKLSEDLEKHGFEITRFKTGTPPRVNSHTIDYSKTEIQPGDDNPKSFSYETTEQITDQI
PCWLTYTNEFTHQVINDNLELSSMYSGMKRGTGPRYCPSIEDKIVRFNDKPRHQIFLEPEGRDTEEVYVQGLSTSLPESI
QHEMIKSVPGLEKAEIMRAGYAIEYDAVVPTQLWPTLETKRLPGLFTAGQINGTSGYEEAAGQGLMAGMNAAAKSLNKEP
IILDRSQAYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDIGYDYGLITEERYQRFTEKKQQIEQEKKRL
SKIIIKPTEDIQDIMEKAGASRLKEAIKAYDLMKRPELSYDIVDQMIESNLELSEEVKEQVAIQIKYEGYIKKAKEQVDR
MLKMEDKKIPEDIDYDAINGIATEAVEKLKKVRPLSVGQASRISGVNPADVSILLVYIEQGSIAKVAND

Sequences:

>Translated_629_residues
MTYNAGNYDVIVVGAGHAGVESAYAAARMGAKTLMLTLNLDMIAFMPCNPSIGGPAKGIVVREIDALGGIMGKVIDKSYI
QMRMLNTGKGPAVQALRAQADKPIYMKEMKHLMENEENLTIRQGMVNELIVEDGICKGVITETHAAYYADSVIVTTGTFM
RGKVLMGDLEYESGPNNQRVSIKLSEDLEKHGFEITRFKTGTPPRVNSHTIDYSKTEIQPGDDNPKSFSYETTEQITDQI
PCWLTYTNEFTHQVINDNLELSSMYSGMKRGTGPRYCPSIEDKIVRFNDKPRHQIFLEPEGRDTEEVYVQGLSTSLPESI
QHEMIKSVPGLEKAEIMRAGYAIEYDAVVPTQLWPTLETKRLPGLFTAGQINGTSGYEEAAGQGLMAGMNAAAKSLNKEP
IILDRSQAYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDIGYDYGLITEERYQRFTEKKQQIEQEKKRL
SKIIIKPTEDIQDIMEKAGASRLKEAIKAYDLMKRPELSYDIVDQMIESNLELSEEVKEQVAIQIKYEGYIKKAKEQVDR
MLKMEDKKIPEDIDYDAINGIATEAVEKLKKVRPLSVGQASRISGVNPADVSILLVYIEQGSIAKVAND
>Mature_628_residues
TYNAGNYDVIVVGAGHAGVESAYAAARMGAKTLMLTLNLDMIAFMPCNPSIGGPAKGIVVREIDALGGIMGKVIDKSYIQ
MRMLNTGKGPAVQALRAQADKPIYMKEMKHLMENEENLTIRQGMVNELIVEDGICKGVITETHAAYYADSVIVTTGTFMR
GKVLMGDLEYESGPNNQRVSIKLSEDLEKHGFEITRFKTGTPPRVNSHTIDYSKTEIQPGDDNPKSFSYETTEQITDQIP
CWLTYTNEFTHQVINDNLELSSMYSGMKRGTGPRYCPSIEDKIVRFNDKPRHQIFLEPEGRDTEEVYVQGLSTSLPESIQ
HEMIKSVPGLEKAEIMRAGYAIEYDAVVPTQLWPTLETKRLPGLFTAGQINGTSGYEEAAGQGLMAGMNAAAKSLNKEPI
ILDRSQAYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDIGYDYGLITEERYQRFTEKKQQIEQEKKRLS
KIIIKPTEDIQDIMEKAGASRLKEAIKAYDLMKRPELSYDIVDQMIESNLELSEEVKEQVAIQIKYEGYIKKAKEQVDRM
LKMEDKKIPEDIDYDAINGIATEAVEKLKKVRPLSVGQASRISGVNPADVSILLVYIEQGSIAKVAND

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=630, Percent_Identity=41.4285714285714, Blast_Score=467, Evalue=1e-131,
Organism=Homo sapiens, GI19882217, Length=655, Percent_Identity=39.8473282442748, Blast_Score=452, Evalue=1e-127,
Organism=Homo sapiens, GI183227703, Length=670, Percent_Identity=38.955223880597, Blast_Score=447, Evalue=1e-125,
Organism=Escherichia coli, GI2367273, Length=623, Percent_Identity=48.7961476725522, Blast_Score=608, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI17534255, Length=623, Percent_Identity=39.9678972712681, Blast_Score=469, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6321202, Length=617, Percent_Identity=40.8427876823339, Blast_Score=455, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24658174, Length=637, Percent_Identity=40.8163265306122, Blast_Score=462, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_OCEIH (Q8EKU3)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_694412.1
- ProteinModelPortal:   Q8EKU3
- SMR:   Q8EKU3
- GeneID:   1016719
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3490
- NMPDR:   fig|221109.1.peg.3494
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   OIHE221109:OB3490-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 70376; Mature: 70245

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 126-126 BINDING 181-181 BINDING 370-370

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYNAGNYDVIVVGAGHAGVESAYAAARMGAKTLMLTLNLDMIAFMPCNPSIGGPAKGIV
CCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCEEEEECCCCCCCCCCCCEE
VREIDALGGIMGKVIDKSYIQMRMLNTGKGPAVQALRAQADKPIYMKEMKHLMENEENLT
EEEHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCEE
IRQGMVNELIVEDGICKGVITETHAAYYADSVIVTTGTFMRGKVLMGDLEYESGPNNQRV
EHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCEEECEEEEEEECCCCCCCCCEE
SIKLSEDLEKHGFEITRFKTGTPPRVNSHTIDYSKTEIQPGDDNPKSFSYETTEQITDQI
EEEEHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCC
PCWLTYTNEFTHQVINDNLELSSMYSGMKRGTGPRYCPSIEDKIVRFNDKPRHQIFLEPE
CEEEEECHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCEEEECCC
GRDTEEVYVQGLSTSLPESIQHEMIKSVPGLEKAEIMRAGYAIEYDAVVPTQLWPTLETK
CCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCHHHCCCCCCC
RLPGLFTAGQINGTSGYEEAAGQGLMAGMNAAAKSLNKEPIILDRSQAYIGVMIDDLVTK
CCCCEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCEEEEHHHHHHHC
GTNEPYRLLTSRAEYRLLLRHDNADLRLTDIGYDYGLITEERYQRFTEKKQQIEQEKKRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SKIIIKPTEDIQDIMEKAGASRLKEAIKAYDLMKRPELSYDIVDQMIESNLELSEEVKEQ
HHEECCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHC
VAIQIKYEGYIKKAKEQVDRMLKMEDKKIPEDIDYDAINGIATEAVEKLKKVRPLSVGQA
EEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
SRISGVNPADVSILLVYIEQGSIAKVAND
HHCCCCCCCCEEEEEEEEECCCEECCCCC
>Mature Secondary Structure 
TYNAGNYDVIVVGAGHAGVESAYAAARMGAKTLMLTLNLDMIAFMPCNPSIGGPAKGIV
CCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCEEEEECCCCCCCCCCCCEE
VREIDALGGIMGKVIDKSYIQMRMLNTGKGPAVQALRAQADKPIYMKEMKHLMENEENLT
EEEHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCEE
IRQGMVNELIVEDGICKGVITETHAAYYADSVIVTTGTFMRGKVLMGDLEYESGPNNQRV
EHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCEEECEEEEEEECCCCCCCCCEE
SIKLSEDLEKHGFEITRFKTGTPPRVNSHTIDYSKTEIQPGDDNPKSFSYETTEQITDQI
EEEEHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCC
PCWLTYTNEFTHQVINDNLELSSMYSGMKRGTGPRYCPSIEDKIVRFNDKPRHQIFLEPE
CEEEEECHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCEEEECCC
GRDTEEVYVQGLSTSLPESIQHEMIKSVPGLEKAEIMRAGYAIEYDAVVPTQLWPTLETK
CCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCHHHCCCCCCC
RLPGLFTAGQINGTSGYEEAAGQGLMAGMNAAAKSLNKEPIILDRSQAYIGVMIDDLVTK
CCCCEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCEEEEHHHHHHHC
GTNEPYRLLTSRAEYRLLLRHDNADLRLTDIGYDYGLITEERYQRFTEKKQQIEQEKKRL
CCCCHHHHHHCCCCEEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SKIIIKPTEDIQDIMEKAGASRLKEAIKAYDLMKRPELSYDIVDQMIESNLELSEEVKEQ
HHEECCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHC
VAIQIKYEGYIKKAKEQVDRMLKMEDKKIPEDIDYDAINGIATEAVEKLKKVRPLSVGQA
EEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
SRISGVNPADVSILLVYIEQGSIAKVAND
HHCCCCCCCCEEEEEEEEECCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376