Click here to switch to the map view.

The map label for this gene is gidB

Identifier: 23100944

GI number: 23100944

Start: 3622961

End: 3623677

Strand: Reverse

Name: gidB

Synonym: OB3489

Alternate gene names: 23100944

Gene position: 3623677-3622961 (Counterclockwise)

Preceding gene: 23100945

Following gene: 23100943

Centisome position: 99.81

GC content: 32.78

Gene sequence:

>717_bases
ATGAAACCAGAACAATTTGTACACGCATTAAAAGAAAAAGGAATAAATTTGTCTGAAAAACAAACAGAACAATTTTACAT
TTATTTCCAGGAATTAGTAGAATGGAATCAAAAAGTAAATTTGACTGCGATTACAGATCAAGAAGAGGTGTATGTAAAGC
ATTTTTATGATTGCGTTACAGCTGCATTTTATCATGATTTTAATCAAGAAATTTCTATTTGTGATATTGGGGCTGGAGCT
GGGTTTCCAAGTATTCCTTTGAAAATTGTCTTTCCTCAATTAAAAATAACCATCGTAGATTCCTTGAAAAAAAGAATTAC
TTTCTTAAACCATTTAGCAACAAAGTTAGAACTATCCAATGTTGCATTTTATCATGATAGAGCTGAAAATTTCGGAAAAA
ATAAAACGTTTCGTGAGAGTTTTGATATAGTAACAGCTCGTGCTGTTGCTCGTATGTCTGTATTAAGTGAGCTTTGTTTA
CCACTTGTTAAAAAGAATGGTGTATTCATTGCGATGAAGGGTGCGCAAGCAAAAGAAGAATTAGAAGTAGGCAAGCCTGC
TATTGAATTATTAGGTGGGGAAGTAGAAGAAATTCATACCTTCTCATTGCCCATAGAAGAAAGTGAAAGGTCGATTATAT
TAATTTCAAAAAAACGTCAAACACCGAAAAAATATCCACGAAAAGCTGGCCTACCAAATAAAGAGCCGATAGAATAA

Upstream 100 bases:

>100_bases
AAGGAAGTATTGCCAAAGTAGCGAATGATTGATTATATAGCGGCGCGTCACCAGTATGCGTCGCTTTTTCCTTATAAAAG
TAGTGTTAAGGGAGGATCTC

Downstream 100 bases:

>100_bases
GAAAACAGACTAATTTTAATTTATCATTTGTTGTATTCTATTATATACTTTTTCATCGATAGATATGGTAAAATAGGTTT
AGCTAATGTAAGTGAAAAAA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKPEQFVHALKEKGINLSEKQTEQFYIYFQELVEWNQKVNLTAITDQEEVYVKHFYDCVTAAFYHDFNQEISICDIGAGA
GFPSIPLKIVFPQLKITIVDSLKKRITFLNHLATKLELSNVAFYHDRAENFGKNKTFRESFDIVTARAVARMSVLSELCL
PLVKKNGVFIAMKGAQAKEELEVGKPAIELLGGEVEEIHTFSLPIEESERSIILISKKRQTPKKYPRKAGLPNKEPIE

Sequences:

>Translated_238_residues
MKPEQFVHALKEKGINLSEKQTEQFYIYFQELVEWNQKVNLTAITDQEEVYVKHFYDCVTAAFYHDFNQEISICDIGAGA
GFPSIPLKIVFPQLKITIVDSLKKRITFLNHLATKLELSNVAFYHDRAENFGKNKTFRESFDIVTARAVARMSVLSELCL
PLVKKNGVFIAMKGAQAKEELEVGKPAIELLGGEVEEIHTFSLPIEESERSIILISKKRQTPKKYPRKAGLPNKEPIE
>Mature_238_residues
MKPEQFVHALKEKGINLSEKQTEQFYIYFQELVEWNQKVNLTAITDQEEVYVKHFYDCVTAAFYHDFNQEISICDIGAGA
GFPSIPLKIVFPQLKITIVDSLKKRITFLNHLATKLELSNVAFYHDRAENFGKNKTFRESFDIVTARAVARMSVLSELCL
PLVKKNGVFIAMKGAQAKEELEVGKPAIELLGGEVEEIHTFSLPIEESERSIILISKKRQTPKKYPRKAGLPNKEPIE

Specific function: Specifically methylates the N7 position of guanosine in position 535 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=212, Percent_Identity=33.4905660377358, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_OCEIH (Q8EKU4)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_694411.1
- ProteinModelPortal:   Q8EKU4
- SMR:   Q8EKU4
- GeneID:   1016172
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3489
- NMPDR:   fig|221109.1.peg.3493
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- ProtClustDB:   PRK00107
- BioCyc:   OIHE221109:OB3489-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 27149; Mature: 27149

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPEQFVHALKEKGINLSEKQTEQFYIYFQELVEWNQKVNLTAITDQEEVYVKHFYDCVT
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHH
AAFYHDFNQEISICDIGAGAGFPSIPLKIVFPQLKITIVDSLKKRITFLNHLATKLELSN
HHHHHHCCCCEEEEEECCCCCCCCCCEEEEECHHEEHHHHHHHHHHHHHHHHHHHHHHHH
VAFYHDRAENFGKNKTFRESFDIVTARAVARMSVLSELCLPLVKKNGVFIAMKGAQAKEE
EEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHH
LEVGKPAIELLGGEVEEIHTFSLPIEESERSIILISKKRQTPKKYPRKAGLPNKEPIE
HHCCCHHHHHHCCCHHHHEEEECCCCCCCCEEEEEECCCCCCHHCCHHCCCCCCCCCC
>Mature Secondary Structure
MKPEQFVHALKEKGINLSEKQTEQFYIYFQELVEWNQKVNLTAITDQEEVYVKHFYDCVT
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHH
AAFYHDFNQEISICDIGAGAGFPSIPLKIVFPQLKITIVDSLKKRITFLNHLATKLELSN
HHHHHHCCCCEEEEEECCCCCCCCCCEEEEECHHEEHHHHHHHHHHHHHHHHHHHHHHHH
VAFYHDRAENFGKNKTFRESFDIVTARAVARMSVLSELCLPLVKKNGVFIAMKGAQAKEE
EEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHH
LEVGKPAIELLGGEVEEIHTFSLPIEESERSIILISKKRQTPKKYPRKAGLPNKEPIE
HHCCCHHHHHHCCCHHHHEEEECCCCCCCCEEEEEECCCCCCHHCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376