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The map label for this gene is yerD [H]

Identifier: 23100863

GI number: 23100863

Start: 3544285

End: 3545886

Strand: Direct

Name: yerD [H]

Synonym: OB3408

Alternate gene names: 23100863

Gene position: 3544285-3545886 (Clockwise)

Preceding gene: 23100862

Following gene: 23100864

Centisome position: 97.62

GC content: 43.26

Gene sequence:

>1602_bases
ATGGAGTTTTCTGCTACCAATATCATTTTGTTGGCGACGTGTTTACTTGTGATTGCTGCTATCATTATTCCAATAGTGCT
AGTTGGCTATATATATTTGAAGGATCGCAAACAAGATCAGCACGCAATATTACGTAACTTTCCGATACTTGGTAAACTAC
GTTATATTTTGGAAAAAAGTGGACCCGAGCTTCGGCAATATCTATTTAATAACGACCGATCTGGAAAACCATTTTCTCGA
AAGGATTATCAAAATATTGTCATGCCTGCGAAATACCAGAAGAATATGCTTGGATTCGGTTCCGTACGTGACTTCGAAAA
GGCCGATTTCTATGTAAAAAATGCCATGTTTCCAAAACAACAGGATGAACTTGAAGTAGATAACACGGAAGAGATTGCTT
CAAAAGTTTACGACATCCAAGAAGATAATCTACTTAGCCGAAAGGAAAGTCTTCAAGATAAATCTATAAAACCGTGGTTA
CTTACCGATGACAATACAGTGGTGATTGGCCCATCTTCCCGAGCTCCCTTTCGTGTAAAAAGTTTGGTCGGAATGTCTGC
AATGAGCTATGGTTCCCTCGGTGACCATGCAATTACAGCACTATCGAAAGGAATCGGCATGGCTGGCGGTGCGTGGATGA
ATACCGGAGAAGGCGGCTTATCCGATTATCATTTAAAAGGAGACACCGATATTATCGCACAAATTGGACCAGGTCTATTC
GGTGTACGTTCTAAAAATGGAGAATTCAGCTGGGAGCTATTAAAAGAAAAGGCAGCGATACCACAAGTAAAAGCCTTTGA
ACTCAAACTTGCCCAAGGAGCGAAAACACGCGGAGGACATGTCGACGCAGAAAAAGTCACAGAAGAAATTGCTCATATTC
GCAACGTCGAGCCTTACCAAGAGATCAATAGTCCAAATCGATTCAACGAATTTGATGATGTACCGTCTATGTTTTCGTTT
ATGGAAAAAATACGTAACCACACCGGGTTACCGGTTGGGATGAAGATCGTCGTCGGCAGTTCGGATTCCTTTGAAGAGAT
TGCATCTTACATGAAAGAATCCGGAATGGGACCTGACTTTATTACCGTTGACGGTTCAGAAGGCGGCACCGGTGCTTCCT
TTCAAGAACTAGCGGATCGTGTGGGATTACCAGTCAAATCAGCGGTAATGATCGTGGATCAAACGTTGAAAAAATATGGC
GTTCGTGAACGCACGAAGATTATTGCCTCTGGAAAACTATTTACCGCAGACCGTATCGCAGTCGTTTTAGCGATGGGCGC
TGACCTGGTAAATGTTGCACGCGCATTTATGATATCAGTAGGCTGCATTATGGCACAAATCTGCCATACGAATCGCTGCC
CCGTCGGCGTCGCCACAACAGATGCGAAGCTGCAAAGAGCACTCGTCATTGATGAAAAACAATATCGTGCACTAAATTTT
CTTGTGACCCTTCGTGAAAGCCTGTTTCGCATATCTGCAGCAGCAGGGTTAACCAGTCCCACGGGCTTTAATCCTGAACA
CATCTCATATGTAGATGAGCAACACCGCGTGAAAAGTTTGGATGACTATTATTTGCAGGGAGAGTATTCAACATCACGAT
AG

Upstream 100 bases:

>100_bases
TTTTCGACTTACACCTCATACTGTCATTCTCCATGTGTATTCCATCCGAAACCGGGTAATTACATAACAGAGACAAATTA
CCTAAAAGGGGATGTATTTC

Downstream 100 bases:

>100_bases
TTTGATTTTGAGCATTTTGTGGAATAACAAATAATACAAATCAAACGAGCAACCGACAAAGGAGCCAAGTATATGTTTGA
AGATCGTGCAGGAAAAGTAC

Product: glutamate synthase

Products: L-glutamine; 2-oxoglutarate; reduced ferredoxin

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MEFSATNIILLATCLLVIAAIIIPIVLVGYIYLKDRKQDQHAILRNFPILGKLRYILEKSGPELRQYLFNNDRSGKPFSR
KDYQNIVMPAKYQKNMLGFGSVRDFEKADFYVKNAMFPKQQDELEVDNTEEIASKVYDIQEDNLLSRKESLQDKSIKPWL
LTDDNTVVIGPSSRAPFRVKSLVGMSAMSYGSLGDHAITALSKGIGMAGGAWMNTGEGGLSDYHLKGDTDIIAQIGPGLF
GVRSKNGEFSWELLKEKAAIPQVKAFELKLAQGAKTRGGHVDAEKVTEEIAHIRNVEPYQEINSPNRFNEFDDVPSMFSF
MEKIRNHTGLPVGMKIVVGSSDSFEEIASYMKESGMGPDFITVDGSEGGTGASFQELADRVGLPVKSAVMIVDQTLKKYG
VRERTKIIASGKLFTADRIAVVLAMGADLVNVARAFMISVGCIMAQICHTNRCPVGVATTDAKLQRALVIDEKQYRALNF
LVTLRESLFRISAAAGLTSPTGFNPEHISYVDEQHRVKSLDDYYLQGEYSTSR

Sequences:

>Translated_533_residues
MEFSATNIILLATCLLVIAAIIIPIVLVGYIYLKDRKQDQHAILRNFPILGKLRYILEKSGPELRQYLFNNDRSGKPFSR
KDYQNIVMPAKYQKNMLGFGSVRDFEKADFYVKNAMFPKQQDELEVDNTEEIASKVYDIQEDNLLSRKESLQDKSIKPWL
LTDDNTVVIGPSSRAPFRVKSLVGMSAMSYGSLGDHAITALSKGIGMAGGAWMNTGEGGLSDYHLKGDTDIIAQIGPGLF
GVRSKNGEFSWELLKEKAAIPQVKAFELKLAQGAKTRGGHVDAEKVTEEIAHIRNVEPYQEINSPNRFNEFDDVPSMFSF
MEKIRNHTGLPVGMKIVVGSSDSFEEIASYMKESGMGPDFITVDGSEGGTGASFQELADRVGLPVKSAVMIVDQTLKKYG
VRERTKIIASGKLFTADRIAVVLAMGADLVNVARAFMISVGCIMAQICHTNRCPVGVATTDAKLQRALVIDEKQYRALNF
LVTLRESLFRISAAAGLTSPTGFNPEHISYVDEQHRVKSLDDYYLQGEYSTSR
>Mature_533_residues
MEFSATNIILLATCLLVIAAIIIPIVLVGYIYLKDRKQDQHAILRNFPILGKLRYILEKSGPELRQYLFNNDRSGKPFSR
KDYQNIVMPAKYQKNMLGFGSVRDFEKADFYVKNAMFPKQQDELEVDNTEEIASKVYDIQEDNLLSRKESLQDKSIKPWL
LTDDNTVVIGPSSRAPFRVKSLVGMSAMSYGSLGDHAITALSKGIGMAGGAWMNTGEGGLSDYHLKGDTDIIAQIGPGLF
GVRSKNGEFSWELLKEKAAIPQVKAFELKLAQGAKTRGGHVDAEKVTEEIAHIRNVEPYQEINSPNRFNEFDDVPSMFSF
MEKIRNHTGLPVGMKIVVGSSDSFEEIASYMKESGMGPDFITVDGSEGGTGASFQELADRVGLPVKSAVMIVDQTLKKYG
VRERTKIIASGKLFTADRIAVVLAMGADLVNVARAFMISVGCIMAQICHTNRCPVGVATTDAKLQRALVIDEKQYRALNF
LVTLRESLFRISAAAGLTSPTGFNPEHISYVDEQHRVKSLDDYYLQGEYSTSR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI308199519, Length=254, Percent_Identity=27.9527559055118, Blast_Score=86, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17570289, Length=319, Percent_Identity=29.4670846394984, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320030, Length=386, Percent_Identity=27.4611398963731, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI28574881, Length=346, Percent_Identity=29.1907514450867, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24665539, Length=346, Percent_Identity=29.1907514450867, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: 1.4.7.1

Molecular weight: Translated: 59032; Mature: 59032

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFSATNIILLATCLLVIAAIIIPIVLVGYIYLKDRKQDQHAILRNFPILGKLRYILEKS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHCCCHHHHHHHHHHCC
GPELRQYLFNNDRSGKPFSRKDYQNIVMPAKYQKNMLGFGSVRDFEKADFYVKNAMFPKQ
CHHHHHHHHCCCCCCCCCCHHCHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCCC
QDELEVDNTEEIASKVYDIQEDNLLSRKESLQDKSIKPWLLTDDNTVVIGPSSRAPFRVK
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEECCCEEEECCCCCCCCHHH
SLVGMSAMSYGSLGDHAITALSKGIGMAGGAWMNTGEGGLSDYHLKGDTDIIAQIGPGLF
HHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHCCCEE
GVRSKNGEFSWELLKEKAAIPQVKAFELKLAQGAKTRGGHVDAEKVTEEIAHIRNVEPYQ
EEECCCCCCHHHHHHHHHCCCCCHHHEEEHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHH
EINSPNRFNEFDDVPSMFSFMEKIRNHTGLPVGMKIVVGSSDSFEEIASYMKESGMGPDF
HCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCE
ITVDGSEGGTGASFQELADRVGLPVKSAVMIVDQTLKKYGVRERTKIIASGKLFTADRIA
EEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHEEECCCEEEHHHHH
VVLAMGADLVNVARAFMISVGCIMAQICHTNRCPVGVATTDAKLQRALVIDEKQYRALNF
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHEECCHHHHHHHH
LVTLRESLFRISAAAGLTSPTGFNPEHISYVDEQHRVKSLDDYYLQGEYSTSR
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCC
>Mature Secondary Structure
MEFSATNIILLATCLLVIAAIIIPIVLVGYIYLKDRKQDQHAILRNFPILGKLRYILEKS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHCCCHHHHHHHHHHCC
GPELRQYLFNNDRSGKPFSRKDYQNIVMPAKYQKNMLGFGSVRDFEKADFYVKNAMFPKQ
CHHHHHHHHCCCCCCCCCCHHCHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCCC
QDELEVDNTEEIASKVYDIQEDNLLSRKESLQDKSIKPWLLTDDNTVVIGPSSRAPFRVK
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEECCCEEEECCCCCCCCHHH
SLVGMSAMSYGSLGDHAITALSKGIGMAGGAWMNTGEGGLSDYHLKGDTDIIAQIGPGLF
HHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHCCCEE
GVRSKNGEFSWELLKEKAAIPQVKAFELKLAQGAKTRGGHVDAEKVTEEIAHIRNVEPYQ
EEECCCCCCHHHHHHHHHCCCCCHHHEEEHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHH
EINSPNRFNEFDDVPSMFSFMEKIRNHTGLPVGMKIVVGSSDSFEEIASYMKESGMGPDF
HCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCE
ITVDGSEGGTGASFQELADRVGLPVKSAVMIVDQTLKKYGVRERTKIIASGKLFTADRIA
EEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHEEECCCEEEHHHHH
VVLAMGADLVNVARAFMISVGCIMAQICHTNRCPVGVATTDAKLQRALVIDEKQYRALNF
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHEECCHHHHHHHH
LVTLRESLFRISAAAGLTSPTGFNPEHISYVDEQHRVKSLDDYYLQGEYSTSR
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; oxidized ferredoxin

Specific reaction: 2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377; 8969499 [H]