Click here to switch to the map view.

The map label for this gene is ydaP [H]

Identifier: 23100864

GI number: 23100864

Start: 3545959

End: 3547680

Strand: Direct

Name: ydaP [H]

Synonym: OB3409

Alternate gene names: 23100864

Gene position: 3545959-3547680 (Clockwise)

Preceding gene: 23100863

Following gene: 23100866

Centisome position: 97.67

GC content: 42.86

Gene sequence:

>1722_bases
ATGTTTGAAGATCGTGCAGGAAAAGTACTCGTAGATTTATTATCCGAATGGGGTGTTGACCATATTTATGGGATGCCCGG
GGATTCTATCAACCAGCTGATGGAAGAGCTACGTAAAGAAAAAGATAAAATGAAGTTTATCCAAGTACGCCACGAAGAAG
CTGGTGCGCTTGCAGCCGCTTCTTACGCCAAACTCACTGGAAAGCTTGGTGTATGTCTGTCCATCGCAGGACCTGGAGCG
ATTCATTTACTTAACGGACTTTACGATGCAAAGAAAGATAGTGCACCGGTGCTAGTACTTGCAGGACAAGTGCCAACAGA
CCAGATTGGAACGGACGCATTCCAAGAAATAAATATGCAGCGTATGTTTGAAGATGTCGCTGTATTTAACGAACAAGTGG
TGTCAGAAGAACAATTACCAGCACTTGTTAATCAAGCGGTGCGCACTGCATATGCAGAAAAAGGACCAGCCGTTCTTACC
ATACCGGATGATATTCCAGCTGCAAAAATCAAGAAACACGTACAAAAGAATGCAGCGATATACGAGGAACCCGAACAAGT
ACCAAGCTCTACGGAAATGGAAAAAGCATTAGAGCTGATTGGAAATGCGAAAAAACCAATTATCTTAGCCGGAACAGGTA
CAAAAGGAGCGAAGCAAGAACTTGAACAGTTTTCGGATAAAATCGCTGCTCCAGTTATTGTTTCCTTGCCAGCAAAAGGC
GTGATAGATGATGATCATCCAAACTGCTTAGGCAACTTAGGAATGATTGGAACCAAACCAGCGTATGAAGCAATGGAAGA
TTCCGATTTGGTTATTCTCATCGGAACGTCATTCCCATATGTAGAGTTTTTACCAGAAGGTGTATCATACATTCAAATTG
ATAATGATCCACTGAAAATCGGGAAGAGATATCCCGTAAACGTTGGAATTGCTGGCGATTCACAAAAATCATTAACCAGG
TTAAACGAACGAATTACTCGAACCGAAAATCGGGATTTCTTAGAAAACAGCCAAGAGAAAATGGCGAAATGGTGGAAAGA
GATGGAGGAAGACGAACAACAAACATCTACACCGATTAAACCACAACAAATCATTGCTGAAGTACAGAAAATCGCAGAAG
ATGACGCAGTACTCTCTGTAGACGTAGGAAATGTGACAGTGTGGGCAGCCAGACACTTTCGTATGAAAACCACACAGAAG
TTCCTTACATCCAGTTGGCTGGCAACGATGGGATGCGGCCTACCAGGTGCCATCGCAAGTAAAATGGCATATTCCGACCG
CCAGGCTATTGCGTTATGCGGCGACGGTGGATTCTCCATGAATATGCAGGACTTTCTTACTGCGGTGAAATACAATTTAC
CAATGGTTGTCGTTGTATTTAATAACGAACGCATTGGCATGATTAAGTACGAGCAAGAAGCAAAAGGAAATCTTGATTAT
AAAACTAATCTCGAAAGCTTTAACTTCGCCCAGTTCGCGGAAAACTGCGGCGGAAAAGGATATCGTGTAGAGAATTTTGA
AGACCTTGGTCCATCTATACGAGCAGCGGCATCCTTCAACAAGCCCGTGATTGTAGATGTCTGTATTGAAGATGAGCCGC
CAATTCCAGGAAAGCTTTCGTACGAACAAGTAACTAGCTACTCCAAACAAACCATTAAGAAACTATTTGAAAAAGGAAAA
CTAGAAGTCCCCCCCATTCGAAAAGGATTGAAACGGATGTAA

Upstream 100 bases:

>100_bases
GCAGGGAGAGTATTCAACATCACGATAGTTTGATTTTGAGCATTTTGTGGAATAACAAATAATACAAATCAAACGAGCAA
CCGACAAAGGAGCCAAGTAT

Downstream 100 bases:

>100_bases
CATAGTAAGAGGCAAACCAACAGTCGGTTTGCCTCTTTCGTTGTATTTTACCTTTCTATTCACCAAAATGAATTATCTTT
TCTACTATTATGGGTTTTAG

Product: pyruvate oxidase

Products: acetyl phosphate; CO2; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MFEDRAGKVLVDLLSEWGVDHIYGMPGDSINQLMEELRKEKDKMKFIQVRHEEAGALAAASYAKLTGKLGVCLSIAGPGA
IHLLNGLYDAKKDSAPVLVLAGQVPTDQIGTDAFQEINMQRMFEDVAVFNEQVVSEEQLPALVNQAVRTAYAEKGPAVLT
IPDDIPAAKIKKHVQKNAAIYEEPEQVPSSTEMEKALELIGNAKKPIILAGTGTKGAKQELEQFSDKIAAPVIVSLPAKG
VIDDDHPNCLGNLGMIGTKPAYEAMEDSDLVILIGTSFPYVEFLPEGVSYIQIDNDPLKIGKRYPVNVGIAGDSQKSLTR
LNERITRTENRDFLENSQEKMAKWWKEMEEDEQQTSTPIKPQQIIAEVQKIAEDDAVLSVDVGNVTVWAARHFRMKTTQK
FLTSSWLATMGCGLPGAIASKMAYSDRQAIALCGDGGFSMNMQDFLTAVKYNLPMVVVVFNNERIGMIKYEQEAKGNLDY
KTNLESFNFAQFAENCGGKGYRVENFEDLGPSIRAAASFNKPVIVDVCIEDEPPIPGKLSYEQVTSYSKQTIKKLFEKGK
LEVPPIRKGLKRM

Sequences:

>Translated_573_residues
MFEDRAGKVLVDLLSEWGVDHIYGMPGDSINQLMEELRKEKDKMKFIQVRHEEAGALAAASYAKLTGKLGVCLSIAGPGA
IHLLNGLYDAKKDSAPVLVLAGQVPTDQIGTDAFQEINMQRMFEDVAVFNEQVVSEEQLPALVNQAVRTAYAEKGPAVLT
IPDDIPAAKIKKHVQKNAAIYEEPEQVPSSTEMEKALELIGNAKKPIILAGTGTKGAKQELEQFSDKIAAPVIVSLPAKG
VIDDDHPNCLGNLGMIGTKPAYEAMEDSDLVILIGTSFPYVEFLPEGVSYIQIDNDPLKIGKRYPVNVGIAGDSQKSLTR
LNERITRTENRDFLENSQEKMAKWWKEMEEDEQQTSTPIKPQQIIAEVQKIAEDDAVLSVDVGNVTVWAARHFRMKTTQK
FLTSSWLATMGCGLPGAIASKMAYSDRQAIALCGDGGFSMNMQDFLTAVKYNLPMVVVVFNNERIGMIKYEQEAKGNLDY
KTNLESFNFAQFAENCGGKGYRVENFEDLGPSIRAAASFNKPVIVDVCIEDEPPIPGKLSYEQVTSYSKQTIKKLFEKGK
LEVPPIRKGLKRM
>Mature_573_residues
MFEDRAGKVLVDLLSEWGVDHIYGMPGDSINQLMEELRKEKDKMKFIQVRHEEAGALAAASYAKLTGKLGVCLSIAGPGA
IHLLNGLYDAKKDSAPVLVLAGQVPTDQIGTDAFQEINMQRMFEDVAVFNEQVVSEEQLPALVNQAVRTAYAEKGPAVLT
IPDDIPAAKIKKHVQKNAAIYEEPEQVPSSTEMEKALELIGNAKKPIILAGTGTKGAKQELEQFSDKIAAPVIVSLPAKG
VIDDDHPNCLGNLGMIGTKPAYEAMEDSDLVILIGTSFPYVEFLPEGVSYIQIDNDPLKIGKRYPVNVGIAGDSQKSLTR
LNERITRTENRDFLENSQEKMAKWWKEMEEDEQQTSTPIKPQQIIAEVQKIAEDDAVLSVDVGNVTVWAARHFRMKTTQK
FLTSSWLATMGCGLPGAIASKMAYSDRQAIALCGDGGFSMNMQDFLTAVKYNLPMVVVVFNNERIGMIKYEQEAKGNLDY
KTNLESFNFAQFAENCGGKGYRVENFEDLGPSIRAAASFNKPVIVDVCIEDEPPIPGKLSYEQVTSYSKQTIKKLFEKGK
LEVPPIRKGLKRM

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=575, Percent_Identity=26.2608695652174, Blast_Score=176, Evalue=8e-44,
Organism=Homo sapiens, GI21361361, Length=545, Percent_Identity=21.8348623853211, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1787096, Length=544, Percent_Identity=34.0073529411765, Blast_Score=345, Evalue=5e-96,
Organism=Escherichia coli, GI87081685, Length=558, Percent_Identity=30.1075268817204, Blast_Score=247, Evalue=2e-66,
Organism=Escherichia coli, GI1790104, Length=549, Percent_Identity=28.0510018214936, Blast_Score=228, Evalue=9e-61,
Organism=Escherichia coli, GI1786717, Length=565, Percent_Identity=27.6106194690265, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1788716, Length=553, Percent_Identity=23.6889692585895, Blast_Score=132, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=551, Percent_Identity=25.4083484573503, Blast_Score=146, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=551, Percent_Identity=25.4083484573503, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=552, Percent_Identity=22.463768115942, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=564, Percent_Identity=29.2553191489362, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6320816, Length=556, Percent_Identity=23.7410071942446, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6321524, Length=497, Percent_Identity=22.5352112676056, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323073, Length=491, Percent_Identity=22.1995926680244, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=490, Percent_Identity=22.0408163265306, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320123, Length=534, Percent_Identity=17.9775280898876, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19922626, Length=564, Percent_Identity=24.468085106383, Blast_Score=170, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.3.3

Molecular weight: Translated: 63207; Mature: 63207

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEDRAGKVLVDLLSEWGVDHIYGMPGDSINQLMEELRKEKDKMKFIQVRHEEAGALAAA
CCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SYAKLTGKLGVCLSIAGPGAIHLLNGLYDAKKDSAPVLVLAGQVPTDQIGTDAFQEINMQ
HHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHH
RMFEDVAVFNEQVVSEEQLPALVNQAVRTAYAEKGPAVLTIPDDIPAAKIKKHVQKNAAI
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCC
YEEPEQVPSSTEMEKALELIGNAKKPIILAGTGTKGAKQELEQFSDKIAAPVIVSLPAKG
CCCHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCCC
VIDDDHPNCLGNLGMIGTKPAYEAMEDSDLVILIGTSFPYVEFLPEGVSYIQIDNDPLKI
CCCCCCCCHHHCCCCCCCCCHHHHCCCCCEEEEEECCCCHHHHHCCCCEEEEECCCHHHC
GKRYPVNVGIAGDSQKSLTRLNERITRTENRDFLENSQEKMAKWWKEMEEDEQQTSTPIK
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHCCCCC
PQQIIAEVQKIAEDDAVLSVDVGNVTVWAARHFRMKTTQKFLTSSWLATMGCGLPGAIAS
HHHHHHHHHHHCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KMAYSDRQAIALCGDGGFSMNMQDFLTAVKYNLPMVVVVFNNERIGMIKYEQEAKGNLDY
HHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCCCE
KTNLESFNFAQFAENCGGKGYRVENFEDLGPSIRAAASFNKPVIVDVCIEDEPPIPGKLS
ECCCCCCHHHHHHHHCCCCCEEECCHHHCCCHHHHHHCCCCCEEEEEEECCCCCCCCCCC
YEQVTSYSKQTIKKLFEKGKLEVPPIRKGLKRM
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MFEDRAGKVLVDLLSEWGVDHIYGMPGDSINQLMEELRKEKDKMKFIQVRHEEAGALAAA
CCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SYAKLTGKLGVCLSIAGPGAIHLLNGLYDAKKDSAPVLVLAGQVPTDQIGTDAFQEINMQ
HHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHH
RMFEDVAVFNEQVVSEEQLPALVNQAVRTAYAEKGPAVLTIPDDIPAAKIKKHVQKNAAI
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCCCC
YEEPEQVPSSTEMEKALELIGNAKKPIILAGTGTKGAKQELEQFSDKIAAPVIVSLPAKG
CCCHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEECCCCC
VIDDDHPNCLGNLGMIGTKPAYEAMEDSDLVILIGTSFPYVEFLPEGVSYIQIDNDPLKI
CCCCCCCCHHHCCCCCCCCCHHHHCCCCCEEEEEECCCCHHHHHCCCCEEEEECCCHHHC
GKRYPVNVGIAGDSQKSLTRLNERITRTENRDFLENSQEKMAKWWKEMEEDEQQTSTPIK
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHCCCCC
PQQIIAEVQKIAEDDAVLSVDVGNVTVWAARHFRMKTTQKFLTSSWLATMGCGLPGAIAS
HHHHHHHHHHHCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KMAYSDRQAIALCGDGGFSMNMQDFLTAVKYNLPMVVVVFNNERIGMIKYEQEAKGNLDY
HHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCCCE
KTNLESFNFAQFAENCGGKGYRVENFEDLGPSIRAAASFNKPVIVDVCIEDEPPIPGKLS
ECCCCCCHHHHHHHHCCCCCEEECCHHHCCCHHHHHHCCCCCEEEEEEECCCCCCCCCCC
YEQVTSYSKQTIKKLFEKGKLEVPPIRKGLKRM
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; phosphate; O2; H2O

Specific reaction: pyruvate + phosphate + O2 + H2O = acetyl phosphate + CO2 + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]