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The map label for this gene is cysL [H]

Identifier: 23100862

GI number: 23100862

Start: 3543301

End: 3544176

Strand: Direct

Name: cysL [H]

Synonym: OB3407

Alternate gene names: 23100862

Gene position: 3543301-3544176 (Clockwise)

Preceding gene: 23100860

Following gene: 23100863

Centisome position: 97.6

GC content: 38.81

Gene sequence:

>876_bases
ATGCATATGGAAGAACTGGAGACCTTTATTGTTCTAGCACGCGAGAAAAATTTCACACGAACAGCAACCAAACGCTCACT
GTCTCAACCAACCGTCAGTGTTCATATTAAAAATTTAGAGAACGAATTTGCCACATCGCTTATCCAACGCACGACAAAGC
GTGTTCACCTTACACCTGAAGGGGAGTTATTTTATGAACATGCATTAAAGATATGGAATCAATATCAACAACTCCAGGAA
CATCTTTATCAAAAGAAACAAACTGCTTCCGGCCTGATTCGAGTAGGGGCTAGTTTTACCATCGGTGAATACCTCCTTCC
AGAAGTGATAGCGATATTGCAGAAACAATACGAACAATTAGACTTTTATGTAACGATTGGCAATACCGAGAAAATTATTG
ATTCTGTGCGCAGACTTGAGGTGGATATCGGGCTGGTAGAAGGATCAAATTACACAAAGGACGTGTTGCAAACATCGTTT
AAAGAGGATCGGCTCGTTATTGTTTGCTCGCCCGAATATGAGACTAATTTTAAAAACTTAGCAGATTTACATCATCATAA
ATGGGTAATAAGAGAAGAAGGTTCAGGGACGCGACAATACTTTGACCACCTCGTCCAATCGAATGAGATTCAAGTCAAAT
CAACATTGGTTATTAGTTCAACACAAGGAATAAAGAACAGCGTTACCAACGGATTAGGTTTAGCTCTCTTATCTGAAGCA
ACCATCAAAGAAGAATTAGCACATGGGACACTTAAAATGGTTCCGATTGAATCTCTGTATGAAAAACGAGATTTTTCCTA
TGTCTTAACAAAGGGCTCCCAGTTAAACCGAAATACACAACTATTAATTGATACATTAACCAAGATCGATAGATGA

Upstream 100 bases:

>100_bases
TTCTCTATCTATAATTAGTATAGGCTGAGCGCTTTTATTTTTGAAATAAATATATATAATAATACTTATTAAAAAAGTTA
ATAAAAGAGGGATTTTAACC

Downstream 100 bases:

>100_bases
AAGTGGATTTTTCGACTTACACCTCATACTGTCATTCTCCATGTGTATTCCATCCGAAACCGGGTAATTACATAACAGAG
ACAAATTACCTAAAAGGGGA

Product: transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MHMEELETFIVLAREKNFTRTATKRSLSQPTVSVHIKNLENEFATSLIQRTTKRVHLTPEGELFYEHALKIWNQYQQLQE
HLYQKKQTASGLIRVGASFTIGEYLLPEVIAILQKQYEQLDFYVTIGNTEKIIDSVRRLEVDIGLVEGSNYTKDVLQTSF
KEDRLVIVCSPEYETNFKNLADLHHHKWVIREEGSGTRQYFDHLVQSNEIQVKSTLVISSTQGIKNSVTNGLGLALLSEA
TIKEELAHGTLKMVPIESLYEKRDFSYVLTKGSQLNRNTQLLIDTLTKIDR

Sequences:

>Translated_291_residues
MHMEELETFIVLAREKNFTRTATKRSLSQPTVSVHIKNLENEFATSLIQRTTKRVHLTPEGELFYEHALKIWNQYQQLQE
HLYQKKQTASGLIRVGASFTIGEYLLPEVIAILQKQYEQLDFYVTIGNTEKIIDSVRRLEVDIGLVEGSNYTKDVLQTSF
KEDRLVIVCSPEYETNFKNLADLHHHKWVIREEGSGTRQYFDHLVQSNEIQVKSTLVISSTQGIKNSVTNGLGLALLSEA
TIKEELAHGTLKMVPIESLYEKRDFSYVLTKGSQLNRNTQLLIDTLTKIDR
>Mature_291_residues
MHMEELETFIVLAREKNFTRTATKRSLSQPTVSVHIKNLENEFATSLIQRTTKRVHLTPEGELFYEHALKIWNQYQQLQE
HLYQKKQTASGLIRVGASFTIGEYLLPEVIAILQKQYEQLDFYVTIGNTEKIIDSVRRLEVDIGLVEGSNYTKDVLQTSF
KEDRLVIVCSPEYETNFKNLADLHHHKWVIREEGSGTRQYFDHLVQSNEIQVKSTLVISSTQGIKNSVTNGLGLALLSEA
TIKEELAHGTLKMVPIESLYEKRDFSYVLTKGSQLNRNTQLLIDTLTKIDR

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=265, Percent_Identity=32.0754716981132, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1790399, Length=287, Percent_Identity=27.1777003484321, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081904, Length=299, Percent_Identity=24.0802675585284, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788748, Length=287, Percent_Identity=25.0871080139373, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI87082132, Length=256, Percent_Identity=25, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786401, Length=298, Percent_Identity=24.496644295302, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787601, Length=296, Percent_Identity=24.3243243243243, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081978, Length=260, Percent_Identity=26.5384615384615, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787128, Length=258, Percent_Identity=24.031007751938, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789440, Length=258, Percent_Identity=27.1317829457364, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=24.4897959183673, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789639, Length=305, Percent_Identity=22.2950819672131, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788887, Length=175, Percent_Identity=30.2857142857143, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790783, Length=255, Percent_Identity=24.7058823529412, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI157672245, Length=198, Percent_Identity=22.7272727272727, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787589, Length=262, Percent_Identity=26.7175572519084, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33459; Mature: 33459

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMEELETFIVLAREKNFTRTATKRSLSQPTVSVHIKNLENEFATSLIQRTTKRVHLTPE
CCHHHHHHHHEEECCCCCHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHEEEECCC
GELFYEHALKIWNQYQQLQEHLYQKKQTASGLIRVGASFTIGEYLLPEVIAILQKQYEQL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHE
DFYVTIGNTEKIIDSVRRLEVDIGLVEGSNYTKDVLQTSFKEDRLVIVCSPEYETNFKNL
EEEEEECCHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHH
ADLHHHKWVIREEGSGTRQYFDHLVQSNEIQVKSTLVISSTQGIKNSVTNGLGLALLSEA
HHHHHHCEEEEECCCCHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHCCCHHHHHHHHH
TIKEELAHGTLKMVPIESLYEKRDFSYVLTKGSQLNRNTQLLIDTLTKIDR
HHHHHHHCCCEEEEEHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MHMEELETFIVLAREKNFTRTATKRSLSQPTVSVHIKNLENEFATSLIQRTTKRVHLTPE
CCHHHHHHHHEEECCCCCHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHEEEECCC
GELFYEHALKIWNQYQQLQEHLYQKKQTASGLIRVGASFTIGEYLLPEVIAILQKQYEQL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHE
DFYVTIGNTEKIIDSVRRLEVDIGLVEGSNYTKDVLQTSFKEDRLVIVCSPEYETNFKNL
EEEEEECCHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHH
ADLHHHKWVIREEGSGTRQYFDHLVQSNEIQVKSTLVISSTQGIKNSVTNGLGLALLSEA
HHHHHHCEEEEECCCCHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHCCCHHHHHHHHH
TIKEELAHGTLKMVPIESLYEKRDFSYVLTKGSQLNRNTQLLIDTLTKIDR
HHHHHHHCCCEEEEEHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]