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The map label for this gene is ldh

Identifier: 23100734

GI number: 23100734

Start: 3416070

End: 3417035

Strand: Direct

Name: ldh

Synonym: OB3279

Alternate gene names: 23100734

Gene position: 3416070-3417035 (Clockwise)

Preceding gene: 23100733

Following gene: 23100735

Centisome position: 94.09

GC content: 40.99

Gene sequence:

>966_bases
ATGAGAACAAGTCAACAAGCTGTGAATCGTGTGGTACTTATTGGGGGTGGATCGGTTGGTGTTAGTTATGCATTTGCATT
GATGAATCAAGGTGTGACGGAGGAATTGGCAATTATTGATTTAGATGCAGATAAGGCTCTAGGTGATGTGATGGATTTAA
ATCACGGAAAAGCCTTTGCTCCTTCATTGACGAATGTGTGGTTGGGTGAATACGGGGATTGTAAGGATGCGGATATCGTA
TGTATTTGCGCTGGGGCTAACCAACAGTCTGGGGAAACTCGCTTAGATCTTGTAGAAAAAAACATGAAAATCTTTAAAGA
AATTGTGACTGACGTGATGAATAGTGGCTTCAATGGTATATTTTTAATCGCAACAAATCCCGTCGATATCTTGACACAAG
CGGTTATCTCGTTCAGTGGACTGCCGCCCCATCGTGTGATTGGGAGTGGGACTACGCTTGATACGGCAAGGCTGCGATAT
GAACTAGGAGAGTATTTTCACCTTTCACCGAAAAATATTCATGCTTATATTATCGGTGAACACGGTGATACGGAACTCCC
ACTGTGGAGCACAGCTACTATAGGAACGGTACCGCTTCTCACGTATTTAAATCGAAGTGAATCCTATACAACAGAAGATT
TAGACGACATTTTTACAAATGTACGGGATGCAGCATACCGAATCATTCAGAAAAAAGGAGCTACCTACTATGGTATAGCT
ATGAGTCTTGTCCGGGTAACGGAAGCAATATTAAAAGATGAACACAGTATTTTAACTACCAGCAGCTTTCTTCAAGGTGA
ATATGGTGTAGATAATGTATGTATTGGCGTGCCGACGATTATCAATCGAAATGGGGTTAGTGAAATAATAGAAGTGCCAA
TGAACGAAGATGAACAGAAACAATTTAATCATAGCGTTCAGACGCTTAAGGGAATTTACGAACCTACGATGCAAATGATG
AAATAG

Upstream 100 bases:

>100_bases
TTTTCTAAATTTCAGAATTTATGTTGACATTATTTGTGAAATATTTCACAATAAAGATATGGAAGATATTTCATATTTTA
CCGATAAGGATGTGGTGAGC

Downstream 100 bases:

>100_bases
TATAGAACATGAATGAAGGGGAGCACATCAGCCTGCTTGTACGTTTTTGATTTTTGGTTATGTATTGGGGAGACCTTTTT
TAGACTACGATGTTTTATTT

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRTSQQAVNRVVLIGGGSVGVSYAFALMNQGVTEELAIIDLDADKALGDVMDLNHGKAFAPSLTNVWLGEYGDCKDADIV
CICAGANQQSGETRLDLVEKNMKIFKEIVTDVMNSGFNGIFLIATNPVDILTQAVISFSGLPPHRVIGSGTTLDTARLRY
ELGEYFHLSPKNIHAYIIGEHGDTELPLWSTATIGTVPLLTYLNRSESYTTEDLDDIFTNVRDAAYRIIQKKGATYYGIA
MSLVRVTEAILKDEHSILTTSSFLQGEYGVDNVCIGVPTIINRNGVSEIIEVPMNEDEQKQFNHSVQTLKGIYEPTMQMM
K

Sequences:

>Translated_321_residues
MRTSQQAVNRVVLIGGGSVGVSYAFALMNQGVTEELAIIDLDADKALGDVMDLNHGKAFAPSLTNVWLGEYGDCKDADIV
CICAGANQQSGETRLDLVEKNMKIFKEIVTDVMNSGFNGIFLIATNPVDILTQAVISFSGLPPHRVIGSGTTLDTARLRY
ELGEYFHLSPKNIHAYIIGEHGDTELPLWSTATIGTVPLLTYLNRSESYTTEDLDDIFTNVRDAAYRIIQKKGATYYGIA
MSLVRVTEAILKDEHSILTTSSFLQGEYGVDNVCIGVPTIINRNGVSEIIEVPMNEDEQKQFNHSVQTLKGIYEPTMQMM
K
>Mature_321_residues
MRTSQQAVNRVVLIGGGSVGVSYAFALMNQGVTEELAIIDLDADKALGDVMDLNHGKAFAPSLTNVWLGEYGDCKDADIV
CICAGANQQSGETRLDLVEKNMKIFKEIVTDVMNSGFNGIFLIATNPVDILTQAVISFSGLPPHRVIGSGTTLDTARLRY
ELGEYFHLSPKNIHAYIIGEHGDTELPLWSTATIGTVPLLTYLNRSESYTTEDLDDIFTNVRDAAYRIIQKKGATYYGIA
MSLVRVTEAILKDEHSILTTSSFLQGEYGVDNVCIGVPTIINRNGVSEIIEVPMNEDEQKQFNHSVQTLKGIYEPTMQMM
K

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI5031857, Length=314, Percent_Identity=35.9872611464968, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI260099723, Length=314, Percent_Identity=35.9872611464968, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI15082234, Length=312, Percent_Identity=37.1794871794872, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI47059044, Length=304, Percent_Identity=36.8421052631579, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI221136809, Length=304, Percent_Identity=36.8421052631579, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=36.7213114754098, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=36.7213114754098, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI291575128, Length=307, Percent_Identity=35.5048859934853, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI4557032, Length=307, Percent_Identity=35.5048859934853, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI260099727, Length=201, Percent_Identity=40.2985074626866, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI260099725, Length=201, Percent_Identity=40.2985074626866, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI207028494, Length=224, Percent_Identity=33.0357142857143, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI103472011, Length=293, Percent_Identity=24.5733788395904, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI103472015, Length=185, Percent_Identity=28.1081081081081, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17535107, Length=289, Percent_Identity=33.5640138408304, Blast_Score=190, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17554310, Length=323, Percent_Identity=24.4582043343653, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17136226, Length=308, Percent_Identity=33.7662337662338, Blast_Score=204, Evalue=4e-53,
Organism=Drosophila melanogaster, GI45550422, Length=306, Percent_Identity=29.4117647058824, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24647881, Length=320, Percent_Identity=25.3125, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH_OCEIH (Q8ELF0)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_694201.1
- ProteinModelPortal:   Q8ELF0
- SMR:   Q8ELF0
- GeneID:   1016455
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3279
- NMPDR:   fig|221109.1.peg.3281
- HOGENOM:   HBG566126
- OMA:   MDLNHGV
- ProtClustDB:   CLSK249396
- BioCyc:   OIHE221109:OB3279-MONOMER
- BRENDA:   1.1.1.27
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 35253; Mature: 35253

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00064 L_LDH

Important sites: ACT_SITE 181-181 BINDING 94-94 BINDING 126-126 BINDING 157-157 BINDING 235-235

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQQAVNRVVLIGGGSVGVSYAFALMNQGVTEELAIIDLDADKALGDVMDLNHGKAFA
CCCCHHHHCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCCCC
PSLTNVWLGEYGDCKDADIVCICAGANQQSGETRLDLVEKNMKIFKEIVTDVMNSGFNGI
CCHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
FLIATNPVDILTQAVISFSGLPPHRVIGSGTTLDTARLRYELGEYFHLSPKNIHAYIIGE
EEEECCCHHHHHHHHHHHCCCCCHHEECCCCCCHHHHHHHHHCCEEECCCCCEEEEEEEC
HGDTELPLWSTATIGTVPLLTYLNRSESYTTEDLDDIFTNVRDAAYRIIQKKGATYYGIA
CCCCCCCEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MSLVRVTEAILKDEHSILTTSSFLQGEYGVDNVCIGVPTIINRNGVSEIIEVPMNEDEQK
HHHHHHHHHHHHCCCHHEEHHHHHCCCCCCCCEEECCCHHHCCCCHHHHHHCCCCCCHHH
QFNHSVQTLKGIYEPTMQMMK
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTSQQAVNRVVLIGGGSVGVSYAFALMNQGVTEELAIIDLDADKALGDVMDLNHGKAFA
CCCCHHHHCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCCCC
PSLTNVWLGEYGDCKDADIVCICAGANQQSGETRLDLVEKNMKIFKEIVTDVMNSGFNGI
CCHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
FLIATNPVDILTQAVISFSGLPPHRVIGSGTTLDTARLRYELGEYFHLSPKNIHAYIIGE
EEEECCCHHHHHHHHHHHCCCCCHHEECCCCCCHHHHHHHHHCCEEECCCCCEEEEEEEC
HGDTELPLWSTATIGTVPLLTYLNRSESYTTEDLDDIFTNVRDAAYRIIQKKGATYYGIA
CCCCCCCEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MSLVRVTEAILKDEHSILTTSSFLQGEYGVDNVCIGVPTIINRNGVSEIIEVPMNEDEQK
HHHHHHHHHHHHCCCHHEEHHHHHCCCCCCCCEEECCCHHHCCCCHHHHHHCCCCCCHHH
QFNHSVQTLKGIYEPTMQMMK
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376