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The map label for this gene is panB

Identifier: 23100729

GI number: 23100729

Start: 3410759

End: 3411571

Strand: Direct

Name: panB

Synonym: OB3274

Alternate gene names: 23100729

Gene position: 3410759-3411571 (Clockwise)

Preceding gene: 23100727

Following gene: 23100730

Centisome position: 93.95

GC content: 43.67

Gene sequence:

>813_bases
ATGAAGGCCTTGAAAGATTTCATGCGGATGAAGCATGAAGGAGAAAAAATTTCGATGTTGACTGCTTATGATTATCCGAG
TGCAAAGCAAGCGGAAGCTGCTGAAATAGATATGATTCTTGTCGGTGATTCCCTAGGGATGACGGTACTTGGCTACGAAA
GCACTGTCGATGTAACTCTCGACGATATGAAGCATCACGCGCGTGCAGTGCGCCGTGGCGCAAAGGATACGTATGTTGTC
GTTGATATGCCTTTTGGTACCATCGGCATTGATGCAAGTACCGATACAGCTTTTGCCATTGAATTATACCGAGACACTCA
TGCAAACGCTATAAAAATCGAAGGCGCACATGCCGCTCCTGTTATTAAAAAATGCCATGATATCGGTATTCCTGTCGTTG
CCCATCTTGGGTTGACGCCGCAGAGTTACGGCATCACAGGCTATCAACTACAAGCAACCTCTAAAGAAGCAGCCAAACAG
CTGATTGAGGATGCTAAACTTGTTGAAAAAAGTGGTGCGATTATGCTTGTCTTAGAAGCAATACCAAGTGACCTTGCCCA
TGTAATTACCGAGTCGCTTACGATTCCGGTTATCGGGATAGGCGCAGGTGTAGGAACGAATGGACAAGTCCTTGTGTATC
ATGACGTACTTAATTATGGTGTCGAGCATAAGCCAAAATTTGTAAAGCGTTATGGTGATTTTTCTATTGGTGTAGAATCC
ATAAAAAACTTTCATGACGAGGTGAAGCGACAAGCGTTTCCTACTGAAGAATACACATACAAGAAACAAATTATGAACGA
GGTAGACGAATGA

Upstream 100 bases:

>100_bases
TTTTTCATAGCTAAGCTCCTTTTCAACTATCAAACGCATTGTTAATCTATACAGATAATTCTATAATAAGTACAAGAATA
AAAATAGTAGGTGACGAAAA

Downstream 100 bases:

>100_bases
CAAAACGAATTACGACCATAAAAGAAATGCAACACCTTAGTCGAACACGGAAAGCACATAATAAACAAATTGGATTTGTA
CCAACGATGGGGGCATTGCA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKALKDFMRMKHEGEKISMLTAYDYPSAKQAEAAEIDMILVGDSLGMTVLGYESTVDVTLDDMKHHARAVRRGAKDTYVV
VDMPFGTIGIDASTDTAFAIELYRDTHANAIKIEGAHAAPVIKKCHDIGIPVVAHLGLTPQSYGITGYQLQATSKEAAKQ
LIEDAKLVEKSGAIMLVLEAIPSDLAHVITESLTIPVIGIGAGVGTNGQVLVYHDVLNYGVEHKPKFVKRYGDFSIGVES
IKNFHDEVKRQAFPTEEYTYKKQIMNEVDE

Sequences:

>Translated_270_residues
MKALKDFMRMKHEGEKISMLTAYDYPSAKQAEAAEIDMILVGDSLGMTVLGYESTVDVTLDDMKHHARAVRRGAKDTYVV
VDMPFGTIGIDASTDTAFAIELYRDTHANAIKIEGAHAAPVIKKCHDIGIPVVAHLGLTPQSYGITGYQLQATSKEAAKQ
LIEDAKLVEKSGAIMLVLEAIPSDLAHVITESLTIPVIGIGAGVGTNGQVLVYHDVLNYGVEHKPKFVKRYGDFSIGVES
IKNFHDEVKRQAFPTEEYTYKKQIMNEVDE
>Mature_270_residues
MKALKDFMRMKHEGEKISMLTAYDYPSAKQAEAAEIDMILVGDSLGMTVLGYESTVDVTLDDMKHHARAVRRGAKDTYVV
VDMPFGTIGIDASTDTAFAIELYRDTHANAIKIEGAHAAPVIKKCHDIGIPVVAHLGLTPQSYGITGYQLQATSKEAAKQ
LIEDAKLVEKSGAIMLVLEAIPSDLAHVITESLTIPVIGIGAGVGTNGQVLVYHDVLNYGVEHKPKFVKRYGDFSIGVES
IKNFHDEVKRQAFPTEEYTYKKQIMNEVDE

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=262, Percent_Identity=41.2213740458015, Blast_Score=182, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6319653, Length=277, Percent_Identity=36.8231046931408, Blast_Score=176, Evalue=4e-45,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_OCEIH (Q8ELF4)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_694196.1
- ProteinModelPortal:   Q8ELF4
- SMR:   Q8ELF4
- GeneID:   1018036
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3274
- NMPDR:   fig|221109.1.peg.3276
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   OIHE221109:OB3274-MONOMER
- BRENDA:   2.1.2.11
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29611; Mature: 29611

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: ACT_SITE 179-179 BINDING 82-82 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALKDFMRMKHEGEKISMLTAYDYPSAKQAEAAEIDMILVGDSLGMTVLGYESTVDVTL
CHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCEEEEEEEECCCCEEEEECCCEEEEEH
DDMKHHARAVRRGAKDTYVVVDMPFGTIGIDASTDTAFAIELYRDTHANAIKIEGAHAAP
HHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCCCEEEEEEEECCCCCEEEECCCCCCH
VIKKCHDIGIPVVAHLGLTPQSYGITGYQLQATSKEAAKQLIEDAKLVEKSGAIMLVLEA
HHHHHHHCCCCCEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEHH
IPSDLAHVITESLTIPVIGIGAGVGTNGQVLVYHDVLNYGVEHKPKFVKRYGDFSIGVES
CCHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEEHHHHCCCCCCCHHHHHHCCCCCCHHH
IKNFHDEVKRQAFPTEEYTYKKQIMNEVDE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKALKDFMRMKHEGEKISMLTAYDYPSAKQAEAAEIDMILVGDSLGMTVLGYESTVDVTL
CHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCEEEEEEEECCCCEEEEECCCEEEEEH
DDMKHHARAVRRGAKDTYVVVDMPFGTIGIDASTDTAFAIELYRDTHANAIKIEGAHAAP
HHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCCCCEEEEEEEECCCCCEEEECCCCCCH
VIKKCHDIGIPVVAHLGLTPQSYGITGYQLQATSKEAAKQLIEDAKLVEKSGAIMLVLEA
HHHHHHHCCCCCEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEHH
IPSDLAHVITESLTIPVIGIGAGVGTNGQVLVYHDVLNYGVEHKPKFVKRYGDFSIGVES
CCHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEEHHHHCCCCCCCHHHHHHCCCCCCHHH
IKNFHDEVKRQAFPTEEYTYKKQIMNEVDE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376