Click here to switch to the map view.

The map label for this gene is gntR [H]

Identifier: 23100645

GI number: 23100645

Start: 3320698

End: 3321420

Strand: Reverse

Name: gntR [H]

Synonym: OB3190

Alternate gene names: 23100645

Gene position: 3321420-3320698 (Counterclockwise)

Preceding gene: 23100651

Following gene: 23100644

Centisome position: 91.49

GC content: 35.82

Gene sequence:

>723_bases
ATGAAAGCCTTTTATCCAAGAAAACAAGTCATAAATAGTTCTAAGGGTGAGCAGGTCGTTGCTGAATTAAGAATGCGTAT
CATCTCTCAAGCGATTGAACCTGAAACCGTTTTATCTGAGAATCAATTAGCGAAAGAATTTCAAGTCAGTCGATCTCCAA
TTAGAGAGGCACTAAAAGTGCTTAGTAGTGAAAATATTGTTCGCCCTGAGCGAATGGGAGCAGTAGTAATTGGTATATCC
GAGAAAGACATCGAAGAAATATATGATGTGAGGCTCATGATGGAATCGTTTACTTTTCAACGGTTGTTAGATATGGACAA
TGCATCCTTGATTAATGATCTTGAAAAAGTGGTCGAGATGATGAAGATTGCTATTAAGTATAAAGATGTCGATCAATTTT
CGTTCTTAGATATGGAATTTCATGAAACGATTATCCGATCGATTAATCATCACTATATCGCTATGCTTTGGACAAACTTG
AAGCCAGTAATGGAATGTTTGGTTTTATTATCGATGCGTTATCGTATGCAAGAGGATGAGAATGACTTTGAGCGAATCAT
TGAAAATCATCGTTTAATTGTTGAATCGATCAAGAATAAAGATGCAAACTTGGTGAATAATGCATTTTATAAAAACTTTA
ATGATGTCCAAAATCGTGTAGAACGTCTCTGGAGTGACGATGAGCGAATGAAGATTGTGAGGGAGAGCATTGAATCAAAA
TAA

Upstream 100 bases:

>100_bases
TTCCTTTATTAAATCCAACTAATTCAAGCGTTTACAAACAAAAAAACAGATGTTAGTATACTTGTATACAAGTATTCATC
ATGTCGAGGACGGTGAATTT

Downstream 100 bases:

>100_bases
GTATATGCTTGGTGTCGATATCGGCACAACAAGTACAAAAGCAGTATTATACCGGGAAACGGGCGAAATCATTGCTCAAG
GAAATCACGGATATGATTTA

Product: gluconate operon transcriptional repressor

Products: NA

Alternate protein names: P28 protein [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKAFYPRKQVINSSKGEQVVAELRMRIISQAIEPETVLSENQLAKEFQVSRSPIREALKVLSSENIVRPERMGAVVIGIS
EKDIEEIYDVRLMMESFTFQRLLDMDNASLINDLEKVVEMMKIAIKYKDVDQFSFLDMEFHETIIRSINHHYIAMLWTNL
KPVMECLVLLSMRYRMQEDENDFERIIENHRLIVESIKNKDANLVNNAFYKNFNDVQNRVERLWSDDERMKIVRESIESK

Sequences:

>Translated_240_residues
MKAFYPRKQVINSSKGEQVVAELRMRIISQAIEPETVLSENQLAKEFQVSRSPIREALKVLSSENIVRPERMGAVVIGIS
EKDIEEIYDVRLMMESFTFQRLLDMDNASLINDLEKVVEMMKIAIKYKDVDQFSFLDMEFHETIIRSINHHYIAMLWTNL
KPVMECLVLLSMRYRMQEDENDFERIIENHRLIVESIKNKDANLVNNAFYKNFNDVQNRVERLWSDDERMKIVRESIESK
>Mature_240_residues
MKAFYPRKQVINSSKGEQVVAELRMRIISQAIEPETVLSENQLAKEFQVSRSPIREALKVLSSENIVRPERMGAVVIGIS
EKDIEEIYDVRLMMESFTFQRLLDMDNASLINDLEKVVEMMKIAIKYKDVDQFSFLDMEFHETIIRSINHHYIAMLWTNL
KPVMECLVLLSMRYRMQEDENDFERIIENHRLIVESIKNKDANLVNNAFYKNFNDVQNRVERLWSDDERMKIVRESIESK

Specific function: Transcriptional repressor of the gluconate operon (gntRKPZ), which encodes the proteins for gluconate utilization. Represses mRNA synthesis by binding to the gnt operator; the binding is suppressed by gluconate or glucono-delta-lactone [H]

COG id: COG1802

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787821, Length=209, Percent_Identity=26.3157894736842, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 28414; Mature: 28414

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFYPRKQVINSSKGEQVVAELRMRIISQAIEPETVLSENQLAKEFQVSRSPIREALKV
CCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSENIVRPERMGAVVIGISEKDIEEIYDVRLMMESFTFQRLLDMDNASLINDLEKVVEM
HCCCCCCCHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
MKIAIKYKDVDQFSFLDMEFHETIIRSINHHYIAMLWTNLKPVMECLVLLSMRYRMQEDE
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NDFERIIENHRLIVESIKNKDANLVNNAFYKNFNDVQNRVERLWSDDERMKIVRESIESK
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAFYPRKQVINSSKGEQVVAELRMRIISQAIEPETVLSENQLAKEFQVSRSPIREALKV
CCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSENIVRPERMGAVVIGISEKDIEEIYDVRLMMESFTFQRLLDMDNASLINDLEKVVEM
HCCCCCCCHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
MKIAIKYKDVDQFSFLDMEFHETIIRSINHHYIAMLWTNLKPVMECLVLLSMRYRMQEDE
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NDFERIIENHRLIVESIKNKDANLVNNAFYKNFNDVQNRVERLWSDDERMKIVRESIESK
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8535972 [H]