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The map label for this gene is gltB [H]

Identifier: 23100553

GI number: 23100553

Start: 3222344

End: 3223828

Strand: Reverse

Name: gltB [H]

Synonym: OB3098

Alternate gene names: 23100553

Gene position: 3223828-3222344 (Counterclockwise)

Preceding gene: 23100554

Following gene: 23100552

Centisome position: 88.8

GC content: 41.01

Gene sequence:

>1485_bases
ATGGGAAAACAAACGGGTTTTATGGAGTATGAACGACAAACGCGTCCTGGTAGAAATCCACAAGAGCGTGTTAAAGACTG
GAACGATTATACGGCACCGATGTCAGATGAGGAAGTGCAAAAACAGGGTGCTCGTTGTATGGATTGTGGTGTTCCTACGT
GTCATCAAGGAATTGAGATAGCAGGGGTAACTTCAGGTTGCCCTGTCTATCACTTAATTCCAGAGTGGAATAACTTAGTA
TATGAGGGGAAATGGAGAGAAGCACTTGATAAAGAACATCAAATGAATAATTTCCCTGAATTTACAGGAATCGCATGTCC
GGCCCCTTGTGAAGGTGCATGTGTGTTAGGAATAAATGAGCCTGCGGTAGCTATCCGTACGGTGGAACGTTCGATTATTG
AAAAAGGATTTGAAGAAGGATGGGTTGTACCAAATCCACCAAAAATCCGAACTGGTAAACGTGTAGCAATAGTTGGATCT
GGTCCAGCCGGTTTAGCAGCAGCAGATCAGTTAAACAAAGCAGGACATTCTGTTACAGTTTATGAGCGAGATAAGCGCGT
TGGTGGGCTATTAACATATGGAATTCCAGAAATGAAACTATCTTATGATATTGTAATCAGACGTGTGTCCATCTTAGAAC
AAGAAGGCATTGAGTTTATTACGAATACAGAAGTTGGTAAAGATATTACAAATGAAGAGTTACAAGAAACATATGATTCC
GTTGTACTATGTGGCGGTGCAAGTTTACATCGTGACATGCCAGTAGAAGGTAGTCAGCTTAAAGGAGTTCATCCTGCTAT
GGACTTTTTACATGCCAATGTTGAAAGTCTATTAGATTCTAACTTAGAAGATGGAAGCTATATTTCAGCTAAAGATAAGA
ATGTTATCGTCATTGGTGGTGGTGATACAGGAACGGATTGCCTTGCTACCTCTGTTCGGCATAATTGTAAAAGCCTTACA
CAATTTGATATCTATGAAAAACGTAATCTTTTACGTGATGAAGAAGCAAATCCATGGCCCCAATATCCAAAAATTCATCG
AGTGGAGTATGGTCAAAAAGAAGCGGAAGCACAATTTGGTGAGGATCCACGAGCTTATGCAGTTCAGACTACAAAGTTTG
TAGGCGATGAAAACGGTCATGTGAAAGAAGTGCACACGATTAACGTAAAGACACGTTATGAAGGTGGTAAGAAAATTCGT
GAAGAAATACCAGGAACAGAAAAAGTGTGGCCAGCGGATCTAGTATTAGTTGCTATTGGGTTCCAAGGACCAGATCAAGA
TCTAATCGAATCATTAGATGTTGAAACAACAACACGTTCTACAGTGAAAGCAAAATACGGCGAATTTCAAACGAATATCG
ACGGTGTTTTTGCAGCTGGAGATATGCGACGAGGTCAGAGTCTAATCGTCTGGGCAATAAATGAAGGACGAGGAGCTGCA
CGTGAGTGTGATCGTTTCTTAATGGGAGACACGATACTTCCATAA

Upstream 100 bases:

>100_bases
GCAGAACTACAAGCTTTTGAGGAAACGAATCAAATTAAAGTTCAAAAAGATAAGGTGAACGTGTAAGTACGATTCATGTC
AGCAGAAAGGGAGGCTTTTG

Downstream 100 bases:

>100_bases
TTATTTAATATAAAATGTCTAAAAGCACTCCTGTTAGTCAAAATTAATAGGGAGTGCTTTTAAATTAGCTAAATTCTTAA
AAATCTATTTGATATACAAA

Product: glutamate synthase [NADPH] small subunit

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MGKQTGFMEYERQTRPGRNPQERVKDWNDYTAPMSDEEVQKQGARCMDCGVPTCHQGIEIAGVTSGCPVYHLIPEWNNLV
YEGKWREALDKEHQMNNFPEFTGIACPAPCEGACVLGINEPAVAIRTVERSIIEKGFEEGWVVPNPPKIRTGKRVAIVGS
GPAGLAAADQLNKAGHSVTVYERDKRVGGLLTYGIPEMKLSYDIVIRRVSILEQEGIEFITNTEVGKDITNEELQETYDS
VVLCGGASLHRDMPVEGSQLKGVHPAMDFLHANVESLLDSNLEDGSYISAKDKNVIVIGGGDTGTDCLATSVRHNCKSLT
QFDIYEKRNLLRDEEANPWPQYPKIHRVEYGQKEAEAQFGEDPRAYAVQTTKFVGDENGHVKEVHTINVKTRYEGGKKIR
EEIPGTEKVWPADLVLVAIGFQGPDQDLIESLDVETTTRSTVKAKYGEFQTNIDGVFAAGDMRRGQSLIVWAINEGRGAA
RECDRFLMGDTILP

Sequences:

>Translated_494_residues
MGKQTGFMEYERQTRPGRNPQERVKDWNDYTAPMSDEEVQKQGARCMDCGVPTCHQGIEIAGVTSGCPVYHLIPEWNNLV
YEGKWREALDKEHQMNNFPEFTGIACPAPCEGACVLGINEPAVAIRTVERSIIEKGFEEGWVVPNPPKIRTGKRVAIVGS
GPAGLAAADQLNKAGHSVTVYERDKRVGGLLTYGIPEMKLSYDIVIRRVSILEQEGIEFITNTEVGKDITNEELQETYDS
VVLCGGASLHRDMPVEGSQLKGVHPAMDFLHANVESLLDSNLEDGSYISAKDKNVIVIGGGDTGTDCLATSVRHNCKSLT
QFDIYEKRNLLRDEEANPWPQYPKIHRVEYGQKEAEAQFGEDPRAYAVQTTKFVGDENGHVKEVHTINVKTRYEGGKKIR
EEIPGTEKVWPADLVLVAIGFQGPDQDLIESLDVETTTRSTVKAKYGEFQTNIDGVFAAGDMRRGQSLIVWAINEGRGAA
RECDRFLMGDTILP
>Mature_493_residues
GKQTGFMEYERQTRPGRNPQERVKDWNDYTAPMSDEEVQKQGARCMDCGVPTCHQGIEIAGVTSGCPVYHLIPEWNNLVY
EGKWREALDKEHQMNNFPEFTGIACPAPCEGACVLGINEPAVAIRTVERSIIEKGFEEGWVVPNPPKIRTGKRVAIVGSG
PAGLAAADQLNKAGHSVTVYERDKRVGGLLTYGIPEMKLSYDIVIRRVSILEQEGIEFITNTEVGKDITNEELQETYDSV
VLCGGASLHRDMPVEGSQLKGVHPAMDFLHANVESLLDSNLEDGSYISAKDKNVIVIGGGDTGTDCLATSVRHNCKSLTQ
FDIYEKRNLLRDEEANPWPQYPKIHRVEYGQKEAEAQFGEDPRAYAVQTTKFVGDENGHVKEVHTINVKTRYEGGKKIRE
EIPGTEKVWPADLVLVAIGFQGPDQDLIESLDVETTTRSTVKAKYGEFQTNIDGVFAAGDMRRGQSLIVWAINEGRGAAR
ECDRFLMGDTILP

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=305, Percent_Identity=23.2786885245902, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI111118981, Length=119, Percent_Identity=36.1344537815126, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI111118983, Length=119, Percent_Identity=36.1344537815126, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1789606, Length=482, Percent_Identity=35.4771784232365, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1788811, Length=472, Percent_Identity=33.8983050847458, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI87082180, Length=483, Percent_Identity=32.0910973084886, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1788468, Length=473, Percent_Identity=24.5243128964059, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789243, Length=431, Percent_Identity=26.2180974477958, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17570289, Length=504, Percent_Identity=46.6269841269841, Blast_Score=450, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI71984108, Length=485, Percent_Identity=23.5051546391753, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17543860, Length=164, Percent_Identity=29.8780487804878, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=500, Percent_Identity=47.4, Blast_Score=471, Evalue=1e-134,
Organism=Drosophila melanogaster, GI28574881, Length=491, Percent_Identity=51.5274949083503, Blast_Score=516, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24665539, Length=491, Percent_Identity=51.5274949083503, Blast_Score=516, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24665547, Length=491, Percent_Identity=51.5274949083503, Blast_Score=514, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24665543, Length=491, Percent_Identity=51.5274949083503, Blast_Score=514, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24640763, Length=348, Percent_Identity=23.8505747126437, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI18858217, Length=348, Percent_Identity=23.8505747126437, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54747; Mature: 54616

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKQTGFMEYERQTRPGRNPQERVKDWNDYTAPMSDEEVQKQGARCMDCGVPTCHQGIEI
CCCCCCCHHHHHHCCCCCCHHHHHCCCCCCCCCCCHHHHHHCCCEEEECCCCCHHCCEEE
AGVTSGCPVYHLIPEWNNLVYEGKWREALDKEHQMNNFPEFTGIACPAPCEGACVLGINE
EEECCCCCEEEECCCCCCEEECCCHHHHHHHHHHHCCCCCCCCEECCCCCCCEEEEECCC
PAVAIRTVERSIIEKGFEEGWVVPNPPKIRTGKRVAIVGSGPAGLAAADQLNKAGHSVTV
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEE
YERDKRVGGLLTYGIPEMKLSYDIVIRRVSILEQEGIEFITNTEVGKDITNEELQETYDS
EECCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCC
VVLCGGASLHRDMPVEGSQLKGVHPAMDFLHANVESLLDSNLEDGSYISAKDKNVIVIGG
EEEECCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEC
GDTGTDCLATSVRHNCKSLTQFDIYEKRNLLRDEEANPWPQYPKIHRVEYGQKEAEAQFG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHCC
EDPRAYAVQTTKFVGDENGHVKEVHTINVKTRYEGGKKIREEIPGTEKVWPADLVLVAIG
CCCCEEEEEEEEEECCCCCCEEEEEEEEEEEECCCCHHHHHHCCCCCCCCCCEEEEEEEC
FQGPDQDLIESLDVETTTRSTVKAKYGEFQTNIDGVFAAGDMRRGQSLIVWAINEGRGAA
CCCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCH
RECDRFLMGDTILP
HHHHHHHCCCCCCC
>Mature Secondary Structure 
GKQTGFMEYERQTRPGRNPQERVKDWNDYTAPMSDEEVQKQGARCMDCGVPTCHQGIEI
CCCCCCHHHHHHCCCCCCHHHHHCCCCCCCCCCCHHHHHHCCCEEEECCCCCHHCCEEE
AGVTSGCPVYHLIPEWNNLVYEGKWREALDKEHQMNNFPEFTGIACPAPCEGACVLGINE
EEECCCCCEEEECCCCCCEEECCCHHHHHHHHHHHCCCCCCCCEECCCCCCCEEEEECCC
PAVAIRTVERSIIEKGFEEGWVVPNPPKIRTGKRVAIVGSGPAGLAAADQLNKAGHSVTV
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEE
YERDKRVGGLLTYGIPEMKLSYDIVIRRVSILEQEGIEFITNTEVGKDITNEELQETYDS
EECCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCC
VVLCGGASLHRDMPVEGSQLKGVHPAMDFLHANVESLLDSNLEDGSYISAKDKNVIVIGG
EEEECCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEC
GDTGTDCLATSVRHNCKSLTQFDIYEKRNLLRDEEANPWPQYPKIHRVEYGQKEAEAQFG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHCC
EDPRAYAVQTTKFVGDENGHVKEVHTINVKTRYEGGKKIREEIPGTEKVWPADLVLVAIG
CCCCEEEEEEEEEECCCCCCEEEEEEEEEEEECCCCHHHHHHCCCCCCCCCCEEEEEEEC
FQGPDQDLIESLDVETTTRSTVKAKYGEFQTNIDGVFAAGDMRRGQSLIVWAINEGRGAA
CCCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCH
RECDRFLMGDTILP
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]