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The map label for this gene is rlmN

Identifier: 23100552

GI number: 23100552

Start: 3221125

End: 3222192

Strand: Reverse

Name: rlmN

Synonym: OB3097

Alternate gene names: 23100552

Gene position: 3222192-3221125 (Counterclockwise)

Preceding gene: 23100553

Following gene: 23100551

Centisome position: 88.75

GC content: 35.39

Gene sequence:

>1068_bases
ATGAGTAAATCATCTATATATGGCTTAACATATGAAAAGTTAAAAGATTGGTTGATCGAACATGGTGAGAAGCGTTTCCG
TGCCGAACAAGTATGGAATTGGCTTTATAAGAAGAGAATTAATAGCTTTGACGAAATGAATAACGTGAATCAATCAGCTA
TTCAGTTATTGAAAGATAATTTTGTGCTACACACAATGGGGGAGGAAATTCGCCAAGAGTCACAAGATGGTACTATAAAG
TTCTTATTTAAATTAGAAGATGGAAACTTAATTGAGACAGTATTGATGCGTTTTCATTACGGCTTATCGGTTTGTGTAAC
TACTCAGGTCGGTTGTAATATTGGTTGTACCTTCTGTGCTAGTGGTCTGTTAAGAAAGAGTAGGGACCTCTCTAGTGGGG
AAGTTGTAGAGCAAATTATGAATGTACAGAAACATCTAGACGAACGCGGTGAAAAGGATAGAGTAAGCCACATAGTTGTC
ATGGGTATTGGTGAACCATTCGATAATTATAATAATCTCATGGACTTCTTATACACAGTAAATGATGATCGTGGTCTAAA
TATTGGTGCAAGACATATTACGGTATCTACAAGTGGATTAGCGCATAAAATTTATGAGTTTGCTGATGATCCAATTCAAG
TAAACCTGGCTATTTCATTACATGCACCGAATGATGAGTTGCGTACGAAAATCATGAAAATTAATCGGGCTTTTCCAATA
GACAAACTGATGAAATCTGTTGATTATTACTTACAGAAGAAGAATCGACGAATTACGTATGAGTATATTATGTTAGATGA
TGTAAATGACCATAAGAAAGAAGCGATCGAACTTGCGAATTTAATTAAAAATCATCGTCATCTAGCATATGTTAATTTAA
TTCCATACAATACGGTAGATGAGCATATTGATTATCGTCGTAGTAAATCAGAAAACATCCAAGCGTTTTATGAGACATTA
ACGGAGCTTGGTATTAACTGTGGTGTTCGTTGGGAAAATGGAGCTGATATTGATGCAGCTTGCGGTCAGCTCAGAAGTAA
ACAAATTAAGAAATCAAAAGCGGTTTAA

Upstream 100 bases:

>100_bases
GAGTGCTTTTAAATTAGCTAAATTCTTAAAAATCTATTTGATATACAAATCTATATCAAGTAATATAGTGTAGGAGTAAA
AATGGAAATGAGGGTTATTG

Downstream 100 bases:

>100_bases
GTTTTATAAGAAAGCTTCCTTTATCAATTAAGGGAGCTTTTTGTATATGGAGGAGAGTTTGATCTATGTTCATTAGAGAG
ATTGAGGAAAAAGATAATCG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSKSSIYGLTYEKLKDWLIEHGEKRFRAEQVWNWLYKKRINSFDEMNNVNQSAIQLLKDNFVLHTMGEEIRQESQDGTIK
FLFKLEDGNLIETVLMRFHYGLSVCVTTQVGCNIGCTFCASGLLRKSRDLSSGEVVEQIMNVQKHLDERGEKDRVSHIVV
MGIGEPFDNYNNLMDFLYTVNDDRGLNIGARHITVSTSGLAHKIYEFADDPIQVNLAISLHAPNDELRTKIMKINRAFPI
DKLMKSVDYYLQKKNRRITYEYIMLDDVNDHKKEAIELANLIKNHRHLAYVNLIPYNTVDEHIDYRRSKSENIQAFYETL
TELGINCGVRWENGADIDAACGQLRSKQIKKSKAV

Sequences:

>Translated_355_residues
MSKSSIYGLTYEKLKDWLIEHGEKRFRAEQVWNWLYKKRINSFDEMNNVNQSAIQLLKDNFVLHTMGEEIRQESQDGTIK
FLFKLEDGNLIETVLMRFHYGLSVCVTTQVGCNIGCTFCASGLLRKSRDLSSGEVVEQIMNVQKHLDERGEKDRVSHIVV
MGIGEPFDNYNNLMDFLYTVNDDRGLNIGARHITVSTSGLAHKIYEFADDPIQVNLAISLHAPNDELRTKIMKINRAFPI
DKLMKSVDYYLQKKNRRITYEYIMLDDVNDHKKEAIELANLIKNHRHLAYVNLIPYNTVDEHIDYRRSKSENIQAFYETL
TELGINCGVRWENGADIDAACGQLRSKQIKKSKAV
>Mature_354_residues
SKSSIYGLTYEKLKDWLIEHGEKRFRAEQVWNWLYKKRINSFDEMNNVNQSAIQLLKDNFVLHTMGEEIRQESQDGTIKF
LFKLEDGNLIETVLMRFHYGLSVCVTTQVGCNIGCTFCASGLLRKSRDLSSGEVVEQIMNVQKHLDERGEKDRVSHIVVM
GIGEPFDNYNNLMDFLYTVNDDRGLNIGARHITVSTSGLAHKIYEFADDPIQVNLAISLHAPNDELRTKIMKINRAFPID
KLMKSVDYYLQKKNRRITYEYIMLDDVNDHKKEAIELANLIKNHRHLAYVNLIPYNTVDEHIDYRRSKSENIQAFYETLT
ELGINCGVRWENGADIDAACGQLRSKQIKKSKAV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=360, Percent_Identity=36.1111111111111, Blast_Score=213, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_OCEIH (Q8ELW7)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_694019.1
- ProteinModelPortal:   Q8ELW7
- SMR:   Q8ELW7
- GeneID:   1017867
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3097
- NMPDR:   fig|221109.1.peg.3100
- HOGENOM:   HBG335345
- OMA:   VQKYFDE
- BioCyc:   OIHE221109:OB3097-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41050; Mature: 40919

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSSIYGLTYEKLKDWLIEHGEKRFRAEQVWNWLYKKRINSFDEMNNVNQSAIQLLKDN
CCCCCEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHCC
FVLHTMGEEIRQESQDGTIKFLFKLEDGNLIETVLMRFHYGLSVCVTTQVGCNIGCTFCA
CCEEHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHH
SGLLRKSRDLSSGEVVEQIMNVQKHLDERGEKDRVSHIVVMGIGEPFDNYNNLMDFLYTV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHCEEEEEECCCCCCHHHHHHHHHHEE
NDDRGLNIGARHITVSTSGLAHKIYEFADDPIQVNLAISLHAPNDELRTKIMKINRAFPI
CCCCCCCCCCEEEEEECCHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCH
DKLMKSVDYYLQKKNRRITYEYIMLDDVNDHKKEAIELANLIKNHRHLAYVNLIPYNTVD
HHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCHH
EHIDYRRSKSENIQAFYETLTELGINCGVRWENGADIDAACGQLRSKQIKKSKAV
HHHHHHHCCCHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKSSIYGLTYEKLKDWLIEHGEKRFRAEQVWNWLYKKRINSFDEMNNVNQSAIQLLKDN
CCCCEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHCC
FVLHTMGEEIRQESQDGTIKFLFKLEDGNLIETVLMRFHYGLSVCVTTQVGCNIGCTFCA
CCEEHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHH
SGLLRKSRDLSSGEVVEQIMNVQKHLDERGEKDRVSHIVVMGIGEPFDNYNNLMDFLYTV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHCEEEEEECCCCCCHHHHHHHHHHEE
NDDRGLNIGARHITVSTSGLAHKIYEFADDPIQVNLAISLHAPNDELRTKIMKINRAFPI
CCCCCCCCCCEEEEEECCHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCH
DKLMKSVDYYLQKKNRRITYEYIMLDDVNDHKKEAIELANLIKNHRHLAYVNLIPYNTVD
HHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCHH
EHIDYRRSKSENIQAFYETLTELGINCGVRWENGADIDAACGQLRSKQIKKSKAV
HHHHHHHCCCHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376