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The map label for this gene is mdeA [H]

Identifier: 23100501

GI number: 23100501

Start: 3159342

End: 3160520

Strand: Reverse

Name: mdeA [H]

Synonym: OB3046

Alternate gene names: 23100501

Gene position: 3160520-3159342 (Counterclockwise)

Preceding gene: 23100506

Following gene: 23100500

Centisome position: 87.05

GC content: 36.39

Gene sequence:

>1179_bases
ATGAAATATAAACATATGGAGACTTCAGTTATTCATGAAGGATACGACTCAACACAGATGCTTGGGAGCTTGACTCCTCC
AATATTTCAAACGTCAACATACACCTTTGACTCGGCAGAACAAGGGGAAGCACGATTTGCCGCAGAGGAAGAAGGATATA
TCTATTCTCGTTTAGGTAATCCAACTGTGCGCATTTTAGAAGAGAAAATTGCAGCATTAGAAGGCGGAGAGCGAGGGTTA
GCTTTTTCATCTGGAATGGCGGCGGTATCTGCTATTTTATTTGCATTAACAAAAGCTAATGATCATATTATTTGCTCTTC
AGGATTATATGGTTGTACTTATGGATTGTTATCAATGATGAAAGAAAAATATAATATTAAAACGGATTATTCCAATATGT
CATCCGAACAGGAATTGGAAAATCAGATAACGAAAGAAACAGTATTAATCTATGTGGAAACACCGATTAATCCTACCATG
CAAGTAATTGATTTAGAATTAGTTGTGAAAGTAGCTAAGAAGCATCAACTTTTAGTAGTGGTAGATAATACTTTTTCCTC
ACCTTACTTGCAACGTCCTTTAGAGATAGGATGTGATTTTGTTATTCATAGTGCAACGAAGTATATTAGTGGACATGGTG
ATGTGATTGCAGGACTTGCGGTTGGAAAAAAAGATATCATGGATACCATTGCTTTAACTACGCAAAAAGATATTGGCGGA
GTTTTGTCTCCTTTTGATGCTTGGTTATTGATTCGAGGATTAAAAACATTACCATTGCGAATGGATAGACATAGTAGTAA
CGCAGAAAAAATAGTTCAACAACTAAAGTTACACAAAGCGGTTAAAAATGTCTATTATCCTGGAGACCCTGATTTTTCTA
ATGTTGCTATCACCAATAAGCAAATGAAACTCGCAGGAGGCGTACTTGCTTTTGAAATCAATGGTGGAAAGAAAGAAGCG
CAAAAAGTGTTAAATCAATTACACTTTATCAAAATTGCTGTTAGCTTAGGGGATGCAGAATCATTAATGGAACATCCAGC
TTCCATGACACATGCTGTAATTCCTGTTGAAGAACGAGAGAAAATGGGCATAACGGAATCTTTATTAAGACTTTCAGTGG
GAATTGAAGCATGGGAGGATATTTGGGAAGATATCCAACAAGCACTTGATAACATCTAA

Upstream 100 bases:

>100_bases
AGTAATACATTAAAAGAACATAAATATCAGGGGTTAGGTGTTACGATCGATCAGTTAGGCTAGAAAGAAACAACCTATTC
TATATAAGAGGGGGATTTTA

Downstream 100 bases:

>100_bases
ATTAATTATAAAAATAAACGAAGTATTCTACAGCTCAATGTTTGTAGATACTTCTTTTTTATTCGTATTAATTCGGGTAC
ACTATTTAAGATAGAAATGG

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKYKHMETSVIHEGYDSTQMLGSLTPPIFQTSTYTFDSAEQGEARFAAEEEGYIYSRLGNPTVRILEEKIAALEGGERGL
AFSSGMAAVSAILFALTKANDHIICSSGLYGCTYGLLSMMKEKYNIKTDYSNMSSEQELENQITKETVLIYVETPINPTM
QVIDLELVVKVAKKHQLLVVVDNTFSSPYLQRPLEIGCDFVIHSATKYISGHGDVIAGLAVGKKDIMDTIALTTQKDIGG
VLSPFDAWLLIRGLKTLPLRMDRHSSNAEKIVQQLKLHKAVKNVYYPGDPDFSNVAITNKQMKLAGGVLAFEINGGKKEA
QKVLNQLHFIKIAVSLGDAESLMEHPASMTHAVIPVEEREKMGITESLLRLSVGIEAWEDIWEDIQQALDNI

Sequences:

>Translated_392_residues
MKYKHMETSVIHEGYDSTQMLGSLTPPIFQTSTYTFDSAEQGEARFAAEEEGYIYSRLGNPTVRILEEKIAALEGGERGL
AFSSGMAAVSAILFALTKANDHIICSSGLYGCTYGLLSMMKEKYNIKTDYSNMSSEQELENQITKETVLIYVETPINPTM
QVIDLELVVKVAKKHQLLVVVDNTFSSPYLQRPLEIGCDFVIHSATKYISGHGDVIAGLAVGKKDIMDTIALTTQKDIGG
VLSPFDAWLLIRGLKTLPLRMDRHSSNAEKIVQQLKLHKAVKNVYYPGDPDFSNVAITNKQMKLAGGVLAFEINGGKKEA
QKVLNQLHFIKIAVSLGDAESLMEHPASMTHAVIPVEEREKMGITESLLRLSVGIEAWEDIWEDIQQALDNI
>Mature_392_residues
MKYKHMETSVIHEGYDSTQMLGSLTPPIFQTSTYTFDSAEQGEARFAAEEEGYIYSRLGNPTVRILEEKIAALEGGERGL
AFSSGMAAVSAILFALTKANDHIICSSGLYGCTYGLLSMMKEKYNIKTDYSNMSSEQELENQITKETVLIYVETPINPTM
QVIDLELVVKVAKKHQLLVVVDNTFSSPYLQRPLEIGCDFVIHSATKYISGHGDVIAGLAVGKKDIMDTIALTTQKDIGG
VLSPFDAWLLIRGLKTLPLRMDRHSSNAEKIVQQLKLHKAVKNVYYPGDPDFSNVAITNKQMKLAGGVLAFEINGGKKEA
QKVLNQLHFIKIAVSLGDAESLMEHPASMTHAVIPVEEREKMGITESLLRLSVGIEAWEDIWEDIQQALDNI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=389, Percent_Identity=40.1028277634961, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI299473759, Length=389, Percent_Identity=35.9897172236504, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI25453487, Length=388, Percent_Identity=34.2783505154639, Blast_Score=193, Evalue=2e-49,
Organism=Escherichia coli, GI1790375, Length=387, Percent_Identity=34.625322997416, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1789383, Length=397, Percent_Identity=23.9294710327456, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17560056, Length=391, Percent_Identity=39.6419437340153, Blast_Score=277, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI17538055, Length=391, Percent_Identity=39.6419437340153, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI71980708, Length=373, Percent_Identity=38.6058981233244, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6319307, Length=390, Percent_Identity=40.5128205128205, Blast_Score=256, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=32.183908045977, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321254, Length=416, Percent_Identity=31.25, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321170, Length=326, Percent_Identity=27.3006134969325, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6321904, Length=398, Percent_Identity=28.643216080402, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19922554, Length=387, Percent_Identity=37.2093023255814, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 43362; Mature: 43362

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKHMETSVIHEGYDSTQMLGSLTPPIFQTSTYTFDSAEQGEARFAAEEEGYIYSRLGN
CCCCHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEECCC
PTVRILEEKIAALEGGERGLAFSSGMAAVSAILFALTKANDHIICSSGLYGCTYGLLSMM
CHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHH
KEKYNIKTDYSNMSSEQELENQITKETVLIYVETPINPTMQVIDLELVVKVAKKHQLLVV
HHHCCCCCCCCCCCHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEE
VDNTFSSPYLQRPLEIGCDFVIHSATKYISGHGDVIAGLAVGKKDIMDTIALTTQKDIGG
EECCCCCHHHCCCHHHCCCCEEHHHHHHHCCCCCEEEEHHCCHHHHHHHHHHHCCHHHCC
VLSPFDAWLLIRGLKTLPLRMDRHSSNAEKIVQQLKLHKAVKNVYYPGDPDFSNVAITNK
CCCHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
QMKLAGGVLAFEINGGKKEAQKVLNQLHFIKIAVSLGDAESLMEHPASMTHAVIPVEERE
EEEEECCEEEEEECCCHHHHHHHHHHHHEEEEEEECCCHHHHHHCCHHHHEEEEECHHHH
KMGITESLLRLSVGIEAWEDIWEDIQQALDNI
HCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYKHMETSVIHEGYDSTQMLGSLTPPIFQTSTYTFDSAEQGEARFAAEEEGYIYSRLGN
CCCCHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEECCC
PTVRILEEKIAALEGGERGLAFSSGMAAVSAILFALTKANDHIICSSGLYGCTYGLLSMM
CHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHH
KEKYNIKTDYSNMSSEQELENQITKETVLIYVETPINPTMQVIDLELVVKVAKKHQLLVV
HHHCCCCCCCCCCCHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEE
VDNTFSSPYLQRPLEIGCDFVIHSATKYISGHGDVIAGLAVGKKDIMDTIALTTQKDIGG
EECCCCCHHHCCCHHHCCCCEEHHHHHHHCCCCCEEEEHHCCHHHHHHHHHHHCCHHHCC
VLSPFDAWLLIRGLKTLPLRMDRHSSNAEKIVQQLKLHKAVKNVYYPGDPDFSNVAITNK
CCCHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
QMKLAGGVLAFEINGGKKEAQKVLNQLHFIKIAVSLGDAESLMEHPASMTHAVIPVEERE
EEEEECCEEEEEECCCHHHHHHHHHHHHEEEEEEECCCHHHHHHCCHHHHEEEEECHHHH
KMGITESLLRLSVGIEAWEDIWEDIQQALDNI
HCCHHHHHHHHHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]