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The map label for this gene is cls

Identifier: 23100500

GI number: 23100500

Start: 3157730

End: 3159169

Strand: Reverse

Name: cls

Synonym: OB3045

Alternate gene names: 23100500

Gene position: 3159169-3157730 (Counterclockwise)

Preceding gene: 23100501

Following gene: 23100499

Centisome position: 87.02

GC content: 32.85

Gene sequence:

>1440_bases
ATGGGAATAACCTCTTTGTTATTAGGACTAACATTTGTTTTAAATATTGCTTTAGCTATTTCAATTATTTTTCTTGAACG
TAAAGATCCAACGTCCTCTTGGGCATGGGTAATGGTCCTTTTATTTATACCCATACTTGGATTCTTTCTTTATTTAATAT
TTGGCAAACCAATCAGTAATAGAAAGATTTTTTCTTGGGATAAGAAGAGCCGTTTGGGAGTAAAGACAACAGTTCAATCT
CAATTGAGACTCTTAGAAGAAAATCAATTTGAATTTAATCAACCAGACCTTATCGAGCATAAGGATCTTGTATATTTACA
TTTGAAAAATGATGAAGCAATTTATACACAGAATAATGGTGTTGATATTTTTACAGATGGTCAAACGAAATTTGATGCTT
TGCTAGAAGATATAGAAAAAGCGAAAAAACATATACATATTCAGTACTATATTATGCGCAGTGATGGCTTAGGAAATAGG
CTTGCAGACATGCTAATAAAAAAAGTAAATGAAGGTGTAGAAGTTAGAGTTTTATATGATGATATGGGATCAAGATCGTT
AAAAAACAGTTATATAAAACGTTTAAAAAGAGCAGGAGTGATGGTCGAGGCATTCTTCCCATCTCGATTCATAGTCAATT
TCAAGATTAATTATCGAAATCACCGTAAGCTAGCGATTATTGATGGATATATTGGATATTTAGGTGGTTTTAATGTAGGC
GATGAATATTTAGGGATTAATAAAAAGTTTGGATATTGGAGAGACACCCATCTTCGTGTGATTGGAGATGCGGTACAAAG
TATGCAAACACGTTTTATCCTAGATTGGAACCAAGCATCGAGGGATACTATTTTATATAATGAAGATTATTATCAAACAG
TATCTGCTGGAAATGTCGGTATGCAAATTGTTACTAGTGGTCCTGATTCAGAATATGAACAAATCAAGAATGGTTATATA
AAGATGATCATGGAGGCAAACGATTATATTTGTATCCAAACTCCTTATTTTATTCCGGATGAAAGTTTAAGGGATGCATT
AAAAATTGCAGTATTATCTGGGGTACATGTTAAAATTATGATACCGAACAAACCTGATCATCCATTTGTATATTGGGCAA
CATTATCGTATTGTGGCGATTTAATTCAAGCTGGTGCTGAAATATTTATTTATCAGAATGGATTTTTACATGCTAAAACA
ATCATTGTGGATGGTAGGATAGCTTCAGTTGGAACAGCTAATATTGATGTACGTAGTTTCCGACTTAATTTCGAAGTAAA
TGGCTTCTTGTATGATTCTGAAGTAGTGAATCGGCTTCAAAATGAGTTCGACGCAGATTTGGAGAAATCTACGCAAATGA
CAAGGAAACTTTATGATCAAAGATCGATAGGAATCCGTTTTAAAGAATCTATATCCAGGTTGATTTCACCTGTTTTATAA

Upstream 100 bases:

>100_bases
TCGGGTACACTATTTAAGATAGAAATGGGTATTATCTAGAAATGCATTTCTGTGCTATTATTATAAAGTGTAATGTTTCT
TCAGTTAGGGGGCAATGACT

Downstream 100 bases:

>100_bases
ATTATAGAGAATAGAATCATGTATACAACATAATGAAAAAGCATAATAGGAGTGAATTTGAATGGAAGCAAAACCTTGTT
CTGCATCACTAGCAATAAAG

Product: cardiolipin synthase

Products: NA

Alternate protein names: CL synthase

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MGITSLLLGLTFVLNIALAISIIFLERKDPTSSWAWVMVLLFIPILGFFLYLIFGKPISNRKIFSWDKKSRLGVKTTVQS
QLRLLEENQFEFNQPDLIEHKDLVYLHLKNDEAIYTQNNGVDIFTDGQTKFDALLEDIEKAKKHIHIQYYIMRSDGLGNR
LADMLIKKVNEGVEVRVLYDDMGSRSLKNSYIKRLKRAGVMVEAFFPSRFIVNFKINYRNHRKLAIIDGYIGYLGGFNVG
DEYLGINKKFGYWRDTHLRVIGDAVQSMQTRFILDWNQASRDTILYNEDYYQTVSAGNVGMQIVTSGPDSEYEQIKNGYI
KMIMEANDYICIQTPYFIPDESLRDALKIAVLSGVHVKIMIPNKPDHPFVYWATLSYCGDLIQAGAEIFIYQNGFLHAKT
IIVDGRIASVGTANIDVRSFRLNFEVNGFLYDSEVVNRLQNEFDADLEKSTQMTRKLYDQRSIGIRFKESISRLISPVL

Sequences:

>Translated_479_residues
MGITSLLLGLTFVLNIALAISIIFLERKDPTSSWAWVMVLLFIPILGFFLYLIFGKPISNRKIFSWDKKSRLGVKTTVQS
QLRLLEENQFEFNQPDLIEHKDLVYLHLKNDEAIYTQNNGVDIFTDGQTKFDALLEDIEKAKKHIHIQYYIMRSDGLGNR
LADMLIKKVNEGVEVRVLYDDMGSRSLKNSYIKRLKRAGVMVEAFFPSRFIVNFKINYRNHRKLAIIDGYIGYLGGFNVG
DEYLGINKKFGYWRDTHLRVIGDAVQSMQTRFILDWNQASRDTILYNEDYYQTVSAGNVGMQIVTSGPDSEYEQIKNGYI
KMIMEANDYICIQTPYFIPDESLRDALKIAVLSGVHVKIMIPNKPDHPFVYWATLSYCGDLIQAGAEIFIYQNGFLHAKT
IIVDGRIASVGTANIDVRSFRLNFEVNGFLYDSEVVNRLQNEFDADLEKSTQMTRKLYDQRSIGIRFKESISRLISPVL
>Mature_478_residues
GITSLLLGLTFVLNIALAISIIFLERKDPTSSWAWVMVLLFIPILGFFLYLIFGKPISNRKIFSWDKKSRLGVKTTVQSQ
LRLLEENQFEFNQPDLIEHKDLVYLHLKNDEAIYTQNNGVDIFTDGQTKFDALLEDIEKAKKHIHIQYYIMRSDGLGNRL
ADMLIKKVNEGVEVRVLYDDMGSRSLKNSYIKRLKRAGVMVEAFFPSRFIVNFKINYRNHRKLAIIDGYIGYLGGFNVGD
EYLGINKKFGYWRDTHLRVIGDAVQSMQTRFILDWNQASRDTILYNEDYYQTVSAGNVGMQIVTSGPDSEYEQIKNGYIK
MIMEANDYICIQTPYFIPDESLRDALKIAVLSGVHVKIMIPNKPDHPFVYWATLSYCGDLIQAGAEIFIYQNGFLHAKTI
IVDGRIASVGTANIDVRSFRLNFEVNGFLYDSEVVNRLQNEFDADLEKSTQMTRKLYDQRSIGIRFKESISRLISPVL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains

Homologues:

Organism=Escherichia coli, GI1787502, Length=487, Percent_Identity=26.2833675564682, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI1787007, Length=346, Percent_Identity=25.7225433526012, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLS_OCEIH (Q8EM16)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693967.1
- ProteinModelPortal:   Q8EM16
- GeneID:   1016694
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3045
- NMPDR:   fig|221109.1.peg.3047
- HOGENOM:   HBG694312
- OMA:   ANIILFV
- BioCyc:   OIHE221109:OB3045-MONOMER
- HAMAP:   MF_00190
- InterPro:   IPR022924
- InterPro:   IPR001736
- SMART:   SM00155

Pfam domain/function: PF00614 PLDc

EC number: 2.7.8.-

Molecular weight: Translated: 55123; Mature: 54992

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS50035 PLD

Important sites: ACT_SITE 221-221 ACT_SITE 223-223 ACT_SITE 228-228 ACT_SITE 397-397 ACT_SITE 399-399 ACT_SITE 404-404

Signals:

None

Transmembrane regions:

HASH(0x1139ba68)-; HASH(0x11839000)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGITSLLLGLTFVLNIALAISIIFLERKDPTSSWAWVMVLLFIPILGFFLYLIFGKPISN
CCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RKIFSWDKKSRLGVKTTVQSQLRLLEENQFEFNQPDLIEHKDLVYLHLKNDEAIYTQNNG
CEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCC
VDIFTDGQTKFDALLEDIEKAKKHIHIQYYIMRSDGLGNRLADMLIKKVNEGVEVRVLYD
EEEEECCCHHHHHHHHHHHHHHHEEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEE
DMGSRSLKNSYIKRLKRAGVMVEAFFPSRFIVNFKINYRNHRKLAIIDGYIGYLGGFNVG
CCCCHHHHHHHHHHHHHCCEEEEEECCCEEEEEEEEECCCCCEEEEEECHHHHHCCCCCC
DEYLGINKKFGYWRDTHLRVIGDAVQSMQTRFILDWNQASRDTILYNEDYYQTVSAGNVG
CHHCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCEEEECCCHHEEECCCCCC
MQIVTSGPDSEYEQIKNGYIKMIMEANDYICIQTPYFIPDESLRDALKIAVLSGVHVKIM
EEEEECCCCHHHHHHCCCEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHEECCCEEEEE
IPNKPDHPFVYWATLSYCGDLIQAGAEIFIYQNGFLHAKTIIVDGRIASVGTANIDVRSF
ECCCCCCCEEEEEHHHHHHHHHHCCCEEEEEECCCEEEEEEEECCEEEECCCCCEEEEEE
RLNFEVNGFLYDSEVVNRLQNEFDADLEKSTQMTRKLYDQRSIGIRFKESISRLISPVL
EEEEEEEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCH
>Mature Secondary Structure 
GITSLLLGLTFVLNIALAISIIFLERKDPTSSWAWVMVLLFIPILGFFLYLIFGKPISN
CHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RKIFSWDKKSRLGVKTTVQSQLRLLEENQFEFNQPDLIEHKDLVYLHLKNDEAIYTQNNG
CEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCC
VDIFTDGQTKFDALLEDIEKAKKHIHIQYYIMRSDGLGNRLADMLIKKVNEGVEVRVLYD
EEEEECCCHHHHHHHHHHHHHHHEEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEE
DMGSRSLKNSYIKRLKRAGVMVEAFFPSRFIVNFKINYRNHRKLAIIDGYIGYLGGFNVG
CCCCHHHHHHHHHHHHHCCEEEEEECCCEEEEEEEEECCCCCEEEEEECHHHHHCCCCCC
DEYLGINKKFGYWRDTHLRVIGDAVQSMQTRFILDWNQASRDTILYNEDYYQTVSAGNVG
CHHCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCEEEECCCHHEEECCCCCC
MQIVTSGPDSEYEQIKNGYIKMIMEANDYICIQTPYFIPDESLRDALKIAVLSGVHVKIM
EEEEECCCCHHHHHHCCCEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHEECCCEEEEE
IPNKPDHPFVYWATLSYCGDLIQAGAEIFIYQNGFLHAKTIIVDGRIASVGTANIDVRSF
ECCCCCCCEEEEEHHHHHHHHHHCCCEEEEEECCCEEEEEEEECCEEEECCCCCEEEEEE
RLNFEVNGFLYDSEVVNRLQNEFDADLEKSTQMTRKLYDQRSIGIRFKESISRLISPVL
EEEEEEEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12235376